Projects funded by the NCN
Essential features of miRNA precursors required for release of homogenous siRNAs from shRNA-miR vectors.
2013/11/N/NZ1/02429
Keywords:
RNA interference miRNA biogenesis shRNA artificial miRNA shRNA-miR
Descriptors:
- NZ1_1: Molecular biology
- NZ1_4: Structural biology
- NZ1_2: Biochemistry
Panel:
NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology
Host institution :
Instytut Chemii Bioorganicznej PAN
woj. wielkopolskie
Principal investigator (from the host institution):
Number of co-investigators in the project: 2
Call: PRELUDIUM 6 - announced on 2013-09-16
Amount awarded: 98 800 PLN
Project start date (Y-m-d): 2014-07-04
Project end date (Y-m-d): 2016-07-03
Project duration:: 24 months (the same as in the proposal)
Project status: Project settled
Information in the final report
- Publication in academic press/journals (3)
- siRNA release from pri-miRNA scaffolds is controlled by the sequence and structure of RNA.Authors:Galka-Marciniak P, Olejniczak M, Starega-Roslan J, Szczesniak MW, Makalowska I, Krzyzosiak WJ.Academic press:Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms (rok: 2016, tom: 1859(4), strony: 639-649), Wydawca: ELSEVIERStatus:PublishedDOI:10.1016/j.bbagrm.2016.02.014 - link to the publication
- Nucleotide sequence of miRNA precursor contributes to cleavage site selection by DicerAuthors:Starega-Roslan J, Galka-Marciniak P, Krzyzosiak WJAcademic press:Nucleic Acids Research (rok: 2015, tom: 43(22), strony: 10939-10951), Wydawca: OXFORD UNIV PRESSStatus:PublishedDOI:10.1093/nar/gkv968 - link to the publication
- Sequence features of Drosha and Dicer cleavage sites affect the complexity of isomiRs.Authors:Starega-Roslan J, Witkos TM, Galka-Marciniak P, Krzyzosiak WJAcademic press:International Journal of Molecular Sciences (rok: 2015, tom: 16(4), strony: 8110-27), Wydawca: MDPI AGStatus:PublishedDOI:10.3390/ijms16048110 - link to the publication