Projects funded by the NCN


Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The effect of ppGpp analogs on RNA polymerase in the aspect of prokariotic and eukariotic transcriptional factors.

2013/10/E/NZ1/00657

Keywords:

RNA polymerase ppGpp transcription

Descriptors:

  • NZ1_1: Molecular biology
  • NZ1_10: Microbiology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Gdański, Wydział Biologii

woj. pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Katarzyna Potrykus 

Number of co-investigators in the project: 5

Call: SONATA BIS 3 - announced on 2013-06-14

Amount awarded: 1 463 820 PLN

Project start date (Y-m-d): 2014-06-05

Project end date (Y-m-d): 2019-06-04

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Spektrofotometr (22 000 PLN)
  2. Wirówka stołowa z chłodzeniem o dużej mocy przerobowej (42 000 PLN)
  3. Zestaw komputerowy: komputer stacjonarny z kolorową drukarką i skanerem (6 500 PLN)
  4. mini-autoklaw (19 500 PLN)
  5. Aparat do elektroforezy poliakrylamidowej białek (3 200 PLN)
  6. Termocykler (44 000 PLN)
  7. aparat do horyzontalnej elektroforezy agarozowej (6 000 PLN)
  8. mikrowirówka (15 000 PLN)
  9. termoblok (9 000 PLN)
  10. termoblok z opcją wytrząsania (12 000 PLN)
  11. mikrowirówka z chłodzeniem (22 000 PLN)
  12. zasilacz (15 000 PLN)
  13. system do dokumentacji płytek TLC (61 500 PLN)
  14. wytrząsarka wodna (40 000 PLN)
  15. Łaźnia wodna (2 900 PLN)

Information in the final report

  • Publication in academic press/journals (5)
  1. Estimates of RelSeq, Mesh1, and SAHMex Hydrolysis of (p)ppGpp and (p)ppApp by Thin Layer Chromatography and NADP/NADH Coupled Assays
    Authors:
    Katarzyna Potrykus, Nathan E. Thomas, Bożena Bruhn-Olszewska, Michał Sobala, Maciej Dylewski, Tamara James and Michael Cashel
    Academic press:
    Frontiers in Microbiology (rok: 2020, tom: 11, strony: 45303), Wydawca: Frontiers Media S.A.
    Status:
    Published
    DOI:
    10.3389/fmicb.2020.581271 - link to the publication
  2. From (p)ppGpp to (pp)pGpp: Characterization of Regulatory Effects of pGpp Synthesized by the Small Alarmone Synthetase of Enterococcus faecalis.
    Authors:
    Gaca AO, Kudrin P, Colomer-Winter C, Beljantseva J, Liu K, Anderson B, Wang JD, Rejman D, Potrykus K, Cashel M, Hauryliuk V, Lemos JA.
    Academic press:
    Journal of Bacteriology (rok: 2015, tom: 197, strony: 2908-19), Wydawca: American Society for Microbiology
    Status:
    Published
    DOI:
    10.1128/JB.00324-15 - link to the publication
  3. Methylobacterium extorquens RSH enzyme synthesizes (p)ppGpp and pppApp in vitro and in vivo, and leads to discovery of pppApp synthesis in Escherichia coli.
    Authors:
    Sobala M, Bruhn-Olszewska B, Cashel M, Potrykus K
    Academic press:
    Frontiers in Microbiology (rok: 2019, tom: 10, strony: 45304), Wydawca: Frontiers Media S.A.
    Status:
    Published
    DOI:
    10.3389/fmicb.2019.00859 - link to the publication
  4. 50th anniversary of discovering "magic spots" – the latest advances in (p)ppGpp research
    Authors:
    Dylewski M, Sobala M, Bruhn-Olszewska B, Potrykus K
    Academic press:
    Postępy Biochemii (rok: 2018, tom: 64 (1), strony: 45299), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
  5. Structure-function comparisons of (p)ppApp vs (p)ppGpp for Escherichia coli RNA polymerase binding sites and for rrnB P1 promoter regulatory responses in vitro.
    Authors:
    Bruhn-Olszewska B, Molodtsov V, Sobala M, Dylewski M, Murakami KS, Cashel M, Potrykus K.
    Academic press:
    Biochimica Biophysica Acta - Gene Regulatory Mechanisms (rok: 2018, tom: 1861(8), strony: 731-742), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagrm.2018.07.005 - link to the publication