Projects funded by the NCN


Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Studies of efficacy of microvesicles derived from genetically modified stem cells as carriers for proangiogenic and cardiomyogenic miRNA.

2013/10/E/NZ3/00750

Keywords:

stem cells MSC iPS microvesicles miRNA zinc finger nucleases (ZFN) angiogenesis cardiomyogenesis regeneration

Descriptors:

  • NZ3_8: Stem cell biology
  • NZ2_1: Molecular genetics

Panel:

NZ3 - Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution :

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Ewa Zuba-Surma 

Number of co-investigators in the project: 5

Call: SONATA BIS 3 - announced on 2013-06-14

Amount awarded: 1 496 570 PLN

Project start date (Y-m-d): 2014-06-10

Project end date (Y-m-d): 2018-06-09

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Inny drobny sprzet laboratoryjny (9 000 PLN)
  2. Mikroskop odwrócony z fluorescencją z kamerą (60 000 PLN)
  3. Pompy próżniowe do laminarów (2 szt.) (5 000 PLN)
  4. System do dokumentacji żeli (45 000 PLN)
  5. Termomikser (8 000 PLN)
  6. System do analiz Western blot (5 000 PLN)
  7. Zestawy standardowych pipet laboratoryjnych (4 szt.) (12 000 PLN)
  8. Standardowa wielofunkcyjna wirówka laboratoryjna (41 000 PLN)
  9. Stołowa wirówka na małe probówki typu eppendorf (8 000 PLN)
  10. Termoblok (3 000 PLN)
  11. Termocykler z opcją gradientu (37 000 PLN)
  12. Standardowy system do elektroforezy (9 000 PLN)
  13. Automatyczna wielofunkcyjna pipeta strzykawkowa (4 000 PLN)
  14. Pipeta wielokanałowa (3 000 PLN)
  15. Przyrząd pipetujący do pipet serologicznych (3 szt.) (6 000 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  • Articles in post-conference publications (1)
  • Book publications / chapters in book publications (1)
  1. Human Induced Pluripotent Stem Cell-derived Microvesicles Transmit RNAs and Proteins to Recipient Mature Heart Cells Modulating Cell Fate and Behavior
    Authors:
    Bobis-Wozowicz Sylwia, Kmiotek Katarzyna, Sekula Malgorzata, Kedracka-Krok Sylwia, Kamycka E, Adamiak M, Jankowska U, Madetko-Talowska A, Sarna Michal, Bik-Multanowski M, Kolcz J, Boruczkowski D, Madeja Zbigniew, Dawn B, Zuba-Surma Ewa K.
    Academic press:
    Stem Cells (rok: 2015, tom: 33 (9), strony: 2748-61), Wydawca: Wiley AlphaMed Press
    Status:
    Published
    DOI:
    10.1002/stem.2078 - link to the publication
  2. Polylactide- and polycaprolactone- based substrates enhance angiogenic potential of human umbilical cord- derived mesenchymal stem cells in vitro - implications for cardiovascular repair
    Authors:
    Sekuła M., Domalik-Pyzik P., Morawska-Chochół A., Bobis-Wozowicz S., Karnas E., Noga S., Boruczkowski D.,Adamiak M., Madeja Z., Chłopek J., Zuba-Surma EK.
    Academic press:
    Mater Sci Eng C (rok: 2017, tom: 77, strony: 521-533), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.msec.2017.03.281 - link to the publication
  3. Induced Pluripotent Stem Cell (iPSC)-Derived Extracellular Vesicles Are Safer and More Effective for Cardiac Repair Than iPSCs.
    Authors:
    Adamiak M, Cheng G, Bobis-Wozowicz S, Zhao L, Kedracka-Krok S, Samanta A, Karnas E, Xuan YT, Skupien-Rabian B, Chen X, Jankowska U, Girgis M, Sekula M, Davani A, Lasota S, Vincent RJ, Sarna M, Newell KL, Wang OL, Dudley N, Madeja Z, Dawn B, Zuba-Surma EK.
    Academic press:
    Circulation Research (rok: 2018, tom: 122, strony: 296-309), Wydawca: American Heart Association
    Status:
    Published
    DOI:
    10.1161/CIRCRESAHA.117.311769 - link to the publication
  4. Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles
    Authors:
    Bobis-Wozowicz S, Kmiotek K, Kania K, Karnas E, Labedz-Maslowska A, Sekula M, Kedracka-Krok S, Kolcz J, Boruczkowski D, Madeja Z, Zuba-Surma EK.
    Academic press:
    J Mol Med (Berl). (rok: 2017, tom: 95, strony: 205–220), Wydawca: Springer International Publishing AG. Part of Springer Nature.
    Status:
    Published
    DOI:
    10.1007/s00109-016-1471-7 - link to the publication
  1. Impact of extracellular vesicles from genetically modified human iPS cells on cardiac cells differentiation in vitro.
    Authors:
    K. Kmiotek, S. Bobis-Wozowicz, E. Karnas, Z. Madeja, E. K. Zuba-Surma
    Conference:
    42nd FEBS Congress (rok: 2017, ), Wydawca: FEBS PRESS (The FEBS Journal)
    Data:
    konferencja 10-14 September
    Status:
    Published
  1. Biomaterials and Stem Cells: Promising Tools in Tissue Engineering and Biomedical Applications
    Authors:
    Sekula M., Zuba-Surma EK.
    Book:
    Biomaterials in Regenerative Medicine (rok: 2017, tom: 1, strony: 361-378), Wydawca: InTech - open science (http://www.intechopen.com)
    Status:
    Published