Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Determinants of carotenoid biosynthesis in carrot: a model system approach

2013/09/B/NZ9/02379

Keywords:

carotenoids biosynthesis gene expression silencing metabolite content Raman spectroscopy carrot Daucus carota

Descriptors:

  • NZ9_4: Horticulture
  • NZ9_13: Other related subjects

Panel:

NZ9 - Fundamentals of applied life sciences and biotechnology: agricultural, forestry, horticulture, animal production and fishery, food and nutrition sciences, industrial biosciences, environmental biotechnology and remediation

Host institution :

Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie, Wydział Biotechnologii i Ogrodnictwa

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Rafał Barański 

Number of co-investigators in the project: 10

Call: OPUS 5 - announced on 2013-03-15

Amount awarded: 689 580 PLN

Project start date (Y-m-d): 2014-02-24

Project end date (Y-m-d): 2017-12-23

Project duration:: 46 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Inkubator z wytrząsaniem (42 000 PLN)
  2. Laptop (3 000 PLN)
  3. Zestaw komputerowy z oprogramowaniem (4 500 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  • Articles in post-conference publications (15)
  • Book publications / chapters in book publications (2)
  1. Efficient CRISPR/Cas9-based genome editing in carrot cells
    Authors:
    Klimek-Chodacka M., Oleszkiewicz T., Lowder L.G., Qi Y., Baranski R.
    Academic press:
    Plant Cell Reports (rok: 2018, tom: 37, strony: 575-586), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00299-018-2252-2 - link to the publication
  2. Unique chromoplast organization and carotenoid gene expression in a model carotenoid-rich carrot callus
    Authors:
    Oleszkiewicz T., Klimek-Chodacka M., Milewska-Hendel A., Zubko M., Stroz D., Kurczynska E., Boba A., Szopa J., Baranski R.
    Academic press:
    Planta (rok: 2018, tom: 248, strony: 1455-1471), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00425-018-2988-5 - link to the publication
  3. Raman, AFM and SNOM high resolution imaging of carotene crystals in a model carrot cell system
    Authors:
    Rygula A., Oleszkiewicz T., Grzebelus E., Pacia M. Z., Baranska M, Baranski R.
    Academic press:
    Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (rok: 2018, tom: 197, strony: 47-55), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.saa.2018.01.054 - link to the publication
  4. Composition and (in)homogeneity of carotenoid crystals in carrot cells revealed by high resolution Raman imaging
    Authors:
    Maciej Roman, Katarzyna M. Marzec, Ewa Grzebelus, Philipp W. Simon, Malgorzata Baranska, Rafal Baranski
    Academic press:
    Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (rok: 2015, tom: 136, strony: 1395-1400), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.026 - link to the publication
  1. Carotenoids in carrot: Raman spectroscopic analysis at organ, tissue and cell levels
    Authors:
    Baranski R.
    Conference:
    AREA project Conference State-of-the-art technologies: challenge for the research in Agricultural and Food Sciences" (rok: 2016, ), Wydawca: University of Belgrade
    Data:
    konferencja 18-20.04
    Status:
    Published
  2. Carrot genome editing using CRISPR based systems
    Authors:
    Baranski R. Klimek-Chodacka M., Oleszkiewicz T.
    Conference:
    2nd International Symposium: Carrot and other Apiaceae (rok: 2018, ), Wydawca: International Society for Horticultural Science, Targi w Krakowie
    Data:
    konferencja 19-22.09
    Status:
    Published
  3. Complex composition of carrot carotenoid crystals
    Authors:
    Roman M., Marzec K.M., Grzebelus E., Simon, P.W., Barańska M., Barański R.
    Conference:
    III National Conference, Genetics and Genomics in Improving Plants - from Model Plant to New Variety (rok: 2014, ), Wydawca: Instytut Genetyki Roślin PAN
    Data:
    konferencja 5-7 listopada
    Status:
    Published
  4. Callus tissue as a new model system for carrot carotenoid research
    Authors:
    Oleszkiewicz T., Klimek-Chodacka M., Milewska-Hendel A., Zubko M., Kurczynska E., Boba A., Szopa J., Baranski R.
    Conference:
    18th International Symposium on Carotenoids (rok: 2017, ), Wydawca: International Carotenoid Society
    Data:
    konferencja 9-14.07
    Status:
    Published
  5. Carrot carotenoids studied in-situ by Raman spectroscopy
    Authors:
    Rafal Baranski
    Conference:
    The 6-th International Conference on Optical Spectroscopy, Laser and their Applications (rok: 2015, ), Wydawca: National Research Centre, Kair, Egipt
    Data:
    konferencja 7-9.04.2015
    Status:
    Published
  6. Impact of exogeneous gibberellic acid on carotenoid composition in carrot cells in vitro
    Authors:
    Oleszkiewicz T., Kostyn A., Klimek-Chodacka M., Szopa J., Barański R.
    Conference:
    XIV Overall Polish in vitro Culture and Plant Biotechnology Conference (rok: 2015, ), Wydawca: Polska Akademia Nauk
    Data:
    konferencja 14-17 września 2015
    Status:
    Published
  7. The impact of exogenous abscisic acid on carotenoid composition and carotenoid pathway gene expression in carrot callus in vitro
    Authors:
    Oleszkiewicz T., Klimek-Chodacka M., Kostyn A., Boba A., Szopa J., Baranski R.
    Conference:
    European Congress on Biotechnology (rok: 2016, ), Wydawca: European Federation of Biotechnology
    Data:
    konferencja 3-6.07
    Status:
    Published
  8. CRISPR/Cas9 induced knockouts of carotenoid gene paralogs in carrot
    Authors:
    Oleszkiewicz T., Klimek-Chodacka M., Lowder L. G., Qi Y., Baranski R.
    Conference:
    International Conference Plant Genome Editing and Genome Engineering (rok: 2017, ), Wydawca: VISCEA
    Data:
    konferencja 3-4.07
    Status:
    Published
  9. Development of zeta-carotene desaturase edited paralogs in carrot using a CRISPR/Cas9 multiplex editing toolbox
    Authors:
    Oleszkiewicz T., Klimek-Chodacka M., Lowder L. G., Qi Y., Baranski R.
    Conference:
    International Conference "Green for Good IV- Biotechnology of Plant Products" (rok: 2017, ), Wydawca: Phytochemical Society of Europe
    Data:
    konferencja 19-22.06
    Status:
    Published
  10. Differential expression of paralog genes in carotenoid biosynthetic pathway in carrot
    Authors:
    Klimek-Chodacka M., Oleszkiewicz T., Baranski R.
    Conference:
    Polski Kongres Genetyki (rok: 2016, ), Wydawca: Polskie Towarzystwo Genetyczne
    Data:
    konferencja 19-22.09
    Status:
    Published
  11. Genetyczne uwarunkowania biosyntezy karotenoidów u marchwi
    Authors:
    Klimek-Chodacka M., Oleszkiewicz T., Barański R.
    Conference:
    Nowe osiągnięcia polskich zespołów badawczych w dziedzinie genetyki, hodowli i biotechnologii roślin (rok: 2016, ), Wydawca: Zachodniopomoorski Uniwersytet Technologiczny w Szczecinie
    Data:
    konferencja 8-10.06
    Status:
    Published
  12. Changes in carotenoid rich carrot callus at transcriptional, histological and ultrastructural levels
    Authors:
    Oleszkiewicz T., Klimek-Chodacka M., Milewska-Hendel A., Zubko M., Stróż D., Kurczynska E., Baranski R.
    Conference:
    X National Conference "In vitro cultures in plant physiology" (rok: 2016, ), Wydawca: Polish Academy of Sciences
    Data:
    konferencja 7-9.12
    Status:
    Published
  13. Development of carotenoid gene knockouts in a model callus system using CRISPR/Cas9 vectors
    Authors:
    Oleszkewicz T., Klimek-Chodacka M., Lowder L. G., Qi Y., Baranski R.
    Conference:
    6th Central European Congress of Life Science EUROBIOTECH (rok: 2017, ), Wydawca: Polska Federacja Biotechnologii, Targi w Krakowie
    Data:
    konferencja 11-14.09
    Status:
    Published
  14. The impact of exogenous plant growth regulators on carotenoid composition and carotenoid pathway gene expression in carrot cells in vitro
    Authors:
    Oleszkiewicz T., Klimek-Chodacka M., Kostyn A., Boba A., Szopa J., Baranski R.
    Conference:
    6th Central European Congress of Life Science EUROBIOTECH (rok: 2017, ), Wydawca: Polska Federacja Biotechnologii, Targi w Krakowie
    Data:
    konferencja 11-14.09
    Status:
    Published
  15. Tissue specific expression of carotenoid pathway genes in carrot leaf, root, and root derived callus cultured in vitro
    Authors:
    Klimek-Chodacka M., Oleszkiewicz T., Kostyn A., Boba A., Szopa J., Barański R.
    Conference:
    European Congress on Biotechnology (rok: 2016, ), Wydawca: European Federation of Biotechnology
    Data:
    konferencja 3-6.07
    Status:
    Published
  1. Carotenoid biosynthesis and regulation in plants
    Authors:
    Baranski R., Cazzonelli C.I.
    Book:
    Carotenoids: Nutrition, Analysis and Technology (rok: 2016, tom: 1, strony: 161-189), Wydawca: John Wiley & Sons Ltd.
    Status:
    Published
    DOI:
    link to the publication
  2. Visual assay for gene editing using a CRISPR/Cas9 system in carrot cells
    Authors:
    Klimek-Chodacka, M., Oleszkiewicz, T., Baranski, R.
    Book:
    Plant Genome Editing with CRISPR Systems: Methods and Protocols, Methods in Molecular Biology (rok: 2019, tom: 1917, strony: 203-215), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    link to the publication

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