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Information on the principal investigator and host institution

Information of the project and the call

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Synthesis and fragmentation of peptides conjugated with betaine derivatives - a search for isobaric markers for quantitative proteomics

2013/09/B/ST4/00277

Keywords:

proteins and peptides mass spectrometry design and synthesis of ionization tags quantitative analysis of proteome iTRAQ

Descriptors:

  • ST4_3: Molecular architecture and structure
  • NZ2_3: Proteomics
  • ST5_23:

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Wrocławski, Wydział Chemii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Zbigniew Szewczuk 

Number of co-investigators in the project: 10

Call: OPUS 5 - announced on 2013-03-15

Amount awarded: 940 000 PLN

Project start date (Y-m-d): 2014-02-10

Project end date (Y-m-d): 2017-07-09

Project duration:: 41 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Wirówka (35 000 PLN)
  2. Zestaw do chromatografii typu "flash" (110 000 PLN)
  3. Mała stołowa wirówka laboratoryjna.
  4. Zestaw do odparowywania rozpuszczalników (wyparka rotacyjna, system próżniowy, cyrkulator chłodzący) (38 000 PLN)
  5. Cyrkulator chłodzący do wyparki.
  6. Generator wodoru (11 008 PLN)
  7. Dwustopniowa olejowa pompa próżniowa odporna chemicznie (15 000 PLN)
  8. Zestaw komputerowy.

Information in the final report

  • Publication in academic press/journals (11)
  • Articles in post-conference publications (5)
  • Book publications / chapters in book publications (1)
  1. Quaternary ammonium isobaric tag for a relative and absolute quantification of peptides
    Authors:
    Setner B., Stefanowicz P., Szewczuk Z.
    Academic press:
    Journal of Mass Spectrometry (rok: 2018, tom: 53, strony: 115-123), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/jms.4040 - link to the publication
  2. The unexpected racemization and hydrogen– deuterium exchange of the hydrogen at the α-carbon of proline analogs containing the 5-azoniaspiro[4.4]nonyl-group
    Authors:
    Setner B., Wierzbicka M., Jerzykiewicz L., Lisowski M., Szewczuk Z.
    Academic press:
    Organic & Biomolecular Chemistry (rok: 2018, tom: 16, strony: 825-831), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c7ob02926h - link to the publication
  3. Preparation of novel deuterated cyclosporin A standards for quantitative LC‐MS analysis
    Authors:
    Bąchor R., Kluczyk A., Stefanowicz P., Szewczuk Z.
    Academic press:
    Journal of Mass Spectrometry (rok: 2017, tom: 52, strony: 817-822), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/jms.4025 - link to the publication
  4. Sensitive detection of charge derivatized peptides at the attomole level using nano-LC-ESI-MRM analysis
    Authors:
    Bąchor R., Mielczarek P., Rudowska M., Silberring J., Szewczuk Z.
    Academic press:
    International Journal of Mass Spectrometry (rok: 2014, tom: 362, strony: 32-38), Wydawca: Elsevier Science
    Status:
    Published
    DOI:
    10.1016/j.ijms.2014.02.018 - link to the publication
  5. Convenient preparation of deuteriumlabeled analogs of peptides containing N-substituted glycines for a stable isotope dilution LC-MS quantitative analysis
    Authors:
    Bąchor R., Dębowski D., Łęgowska A., Stefanowicz P., Rolka K., Szewczuk Z.
    Academic press:
    Journal of Peptide Science (rok: 2015, tom: 21, strony: 819-825), Wydawca: WILEY-BLACKWELL
    Status:
    Published
    DOI:
    10.1002/psc.2823 - link to the publication
  6. Facile synthesis of deuterium-labeled denatonium cation and its application in the quantitative analysis of Bitrex by liquid chromatography-mass spectrometry
    Authors:
    Bąchor, R., Kluczyk A., Stefanowicz P., Szewczuk Z.
    Academic press:
    Analytical and Bioanalytical Chemistry (rok: 2015, tom: 407, strony: 6557-6561), Wydawca: SPRINGER
    Status:
    Published
    DOI:
    10.1007/s00216-015-8815-y - link to the publication
  7. Hydrogen-deuterium exchange of α-carbon protons and fragmentation pathways in N-methylated glycine and alanine-containing peptides derivatized by quaternary ammonium salts.
    Authors:
    Bąchor R., Rudowska M., Kluczyk A., Stefanowicz P., Szewczuk Z.
    Academic press:
    Journal of Mass Spectrometry (rok: 2014, tom: 49, strony: 529-536), Wydawca: John Wiley & Sons
    Status:
    Published
    DOI:
    10.1002/jms.3371 - link to the publication
  8. Peptides Labeled with Pyridinium Salts for Sensitive Detection and Sequencing by Electrospray Tandem Mass Spectrometry
    Authors:
    Waliczek M., Kijewska M., Rudowska M., Setner B., Stefanowicz P., Szewczuk Z.
    Academic press:
    Scientific Reports (rok: 2016, tom: 6, strony: 37720:1-12), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1038/srep37720 - link to the publication
  9. The 5-azoniaspiro[4.4]nonyl group for improved MS peptide analysis: A novel non-fragmenting ionization tag for mass spectrometric sensitive sequencing of peptides*
    Authors:
    Setner B., Rudowska M., Kluczyk A., Stefanowicz P., Szewczuk Z.
    Academic press:
    Analytica Chimica Acta (rok: 2017, tom: 986, strony: 71-81), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.aca.2017.07.029 - link to the publication
  10. Analysis for trace amounts of analytes by electrospray mass spectrometry
    Authors:
    Wierzbicka M., Setner B., Szewczuk Z.
    Academic press:
    Acta Physica Polonica B Proceedings Supplement (rok: 2016, tom: 9, strony: 345-350), Wydawca: Uniwersytet Jagielloński i Polska Akademia Umiejętności
    Status:
    Published
    DOI:
    10.5506/APhysPolBSupp.9.345 - link to the publication
  11. Peptides derivatized with bicyclic quaternary ammonium ionization tags. Sequencing via tandem mass spectrometry.
    Authors:
    Setner B., Rudowska M., Klem E., Cebrat M., Szewczuk Z.
    Academic press:
    Journal of Mass Spectrometry (rok: 2014, tom: 49, strony: 995-1001), Wydawca: John Wiley & Sons
    Status:
    Published
    DOI:
    10.1002/jms.3410 - link to the publication
  1. Deuteration in methylene group of N,N,N-trialkylglycines: markers based on co-elution of isotopomers in chromatographic and mass spectrometric studies
    Authors:
    Kluczyk A., Bąchor R., Setner B., Szewczuk Z.
    Conference:
    33rd European Peptide Symposium (rok: 2015, ), Wydawca: Bulgarian Peptide Society, European Peptide Society
    Data:
    konferencja 31.08.2014-5.09.2014
    Status:
    Published
    DOI:
    link to the publication
  2. Preparation Of Deuterated Analogs Of Peptides With The 5-Azoniaspiro[4.4]Nonyl-Carbonyl Ionization Tag
    Authors:
    Setner B., Wierzbicka M., Lisowski M., Szewczuk Z.
    Conference:
    34th European Peptide Symposium and the 8th International Peptide Symposium (rok: 2017, ), Wydawca: European Peptide Society
    Data:
    konferencja 04 – 09.09.2016
    Status:
    Published
  3. The Consequences Of Hydrogen-Deuterium Exchange In Phosphoniumacetyl-Modified Peptides
    Authors:
    Kluczyk A., Dambinova A., Bachor R., Setner B., Kijewska M., Biernat M., Stefanowicz P., Szewczuk Z.
    Conference:
    34th European Peptide Symposium and the 8th International Peptide Symposium (rok: 2017, ), Wydawca: European Peptide Society
    Data:
    konferencja 04 – 09.09.2016
    Status:
    Published
  4. Application Of Pyrylium Salts For Sensitive Sequencing Of Peptides By Electrospray Tandem Mass Spectrometry
    Authors:
    Waliczek M., Kijewska M., Rudowska M., Setner B.
    Conference:
    34th European Peptide Symposium and the 8th International Peptide Symposium (rok: 2017, ), Wydawca: European Peptide Society
    Data:
    konferencja 04 – 09.09.2016
    Status:
    Published
  5. Peptides labeled by 5-azoniaspiro[4.4]nonyl group for sensitive sequencing by electrospray tandem mass spectrometry
    Authors:
    Setner B., Rudowska M., Wojewska D., Kluczyk A., Stefanowicz P., Szewczuk Z.
    Conference:
    33rd European Peptide Symposium (rok: 2015, ), Wydawca: Bulgarian Peptide Society, European Peptide Society
    Data:
    konferencja 31.08.2014-5.09.2014
    Status:
    Published
  1. Derivatization of peptides for improved detection by mass spectrometry
    Authors:
    Stefanowicz P., Kluczyk A., Szewczuk Z.
    Book:
    Amino Acids, Peptides and Proteins (rok: 2016, tom: 40, strony: 36-74), Wydawca: The Royal Society of Chemistry
    Status:
    Published

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