Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Role of homocysteine in pathogenesis of thrombosis

2013/09/B/NZ5/02794

Keywords:

thrombosis hyperhomocysteinemia protein modification mouse models

Descriptors:

  • NZ5_3: Pathogenesis of human diseases
  • NZ1_1: Molecular biology
  • NZ1_2: Biochemistry

Panel:

NZ5 - Human and animal noninfectious diseases: etiology, mechanisms, diagnosis and treatment of diseases, poisonings and injuries (without neurological diseases)

Host institution :

Uniwersytet Przyrodniczy w Poznaniu

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Hieronim Jakubowski 

Number of co-investigators in the project: 6

Call: OPUS 5 - announced on 2013-03-15

Amount awarded: 999 800 PLN

Project start date (Y-m-d): 2014-02-13

Project end date (Y-m-d): 2017-12-12

Project duration:: 46 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Czytnik wielodetekcyjny Infinite M200Pro firmy Tecan.
  2. Sprzet komputerowy (2 szt.) (10 000 PLN)
  3. Młynek miksujący MM400 firmy Retsch.
  4. Modularny miernik przepływu firmy Transonic (71 000 PLN)
  5. Pionowy regał z 12 klatkami metabolicznymi. (25 000 PLN)
  6. Respirator dla myszy: MouseVent G500 Automatyczny Wentylator, firmy Kent Scientific Corporation (30 000 PLN)
  7. Homogenizator FastPrep-24 z adaptorem na probówki 4,5 mL. (50 000 PLN)

Information in the final report

  • Publication in academic press/journals (15)
  • Articles in post-conference publications (26)
  1. Paraoxonase 1 and dietary hyperhomocysteinemia modulate the expression of mouse proteins involved in liver homeostasis
    Authors:
    Suszyńska-Zajczyk J, Jakubowski H.
    Academic press:
    Acta Biochim Pol. (rok: 2014, tom: 61(4), strony: 815-23), Wydawca: Acta Biochimica Polonica
    Status:
    Published
    DOI:
    brak - link to the publication
  2. The amino acid metabolite homocysteine activates mTORC1 to inhibit autophagy and form abnormal proteins in human neurons and mice
    Authors:
    Khoosheh Khayati, Henri Antikainen, Edward M. Bonder, Gregory F. Weber, Warren D. Kruger, Hieronim Jakubowsk and Radek Dobrowolski
    Academic press:
    FASEB Journal (rok: 2017, tom: 31, strony: 598-609), Wydawca: FASEB
    Status:
    Published
    DOI:
    10.1096/fj.201600915R - link to the publication
  3. l-Arginine and vitamin C attenuate pro-atherogenic effects of high-fat diet on biomarkers of endothelial dysfunction in rats
    Authors:
    Bogdański P, Suliburska J, Szulińska M, Sikora M, Walkowiak J, Jakubowski H.
    Academic press:
    Biomedicine Pharmacotherapy (rok: 2015, tom: 76, strony: 100-106), Wydawca: Elsevier Science
    Status:
    Published
    DOI:
    10.001 - link to the publication
  4. Bleomycin hydrolase and hyperhomocysteinemia modulate the expression of mouse proteins involved in liver homeostasis.
    Authors:
    Suszyńska-Zajczyk J, Wróblewski J, Utyro O, Luczak M, Marczak L, Jakubowski H.
    Academic press:
    Amino Acids (rok: 2014, tom: 46(6), strony: 1471-80), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00726-014-1712-4. - link to the publication
  5. Paraoxonase 1 deficiency and hyperhomocysteinemia alter the expression of mouse kidney proteins involved in renal disease.
    Authors:
    Suszyńska-Zajczyk J, Sikora M, Jakubowski H.
    Academic press:
    Mol Genet Metab. (rok: 2014, tom: 113, strony: 200-206), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ymgme.2014.07.011 - link to the publication
  6. Quantification of urinary S- and N-homocysteinylated protein and homocysteine-thiolactone in mice
    Authors:
    Hieronim Jakubowski
    Academic press:
    Analytical Biochemistry (rok: 2016, tom: 508, strony: 118-123), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ab.2016.06.002 - link to the publication
  7. Aminoacyl-tRNA synthetases and the evolution of coded protein synthesis: the Thioester World.
    Authors:
    Jakubowski H.
    Academic press:
    FEBS Lett. (rok: 2016, tom: 590, strony: 469-81), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/1873-3468.12085 - link to the publication
  8. Inactivation of the Paraoxonase 1 Gene Affects the Expression of Mouse Brain Proteins Involved in Neurodegeneration
    Authors:
    Suszyńska-Zajczyk J, Luczak M, Marczak L, Jakubowski H.
    Academic press:
    Alzheimers Dis. (rok: 2014, tom: 42, strony: 247-260), Wydawca: IOS Press
    Status:
    Published
    DOI:
    10.3233/JAD-132714 - link to the publication
  9. Methionine-induced hyperhomocysteinemia and bleomycin hydrolase deficiency alter the expression of mouse kidney proteins involved in renal disease.
    Authors:
    Suszyńska-Zajczyk J, Utyro O, Jakubowski H.
    Academic press:
    Mol Genet Metab (rok: 2014, tom: 112, strony: 339-46), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ymgme.2014.05.010 - link to the publication
  10. Quantification of homocysteine and cysteine by derivatization with pyridoxal 5'-phosphate and hydrophilic interaction liquid chromatography.
    Authors:
    Glowacki R, Stachniuk J, Borowczyk K, Jakubowski H.
    Academic press:
    Analytical Bioanalytical Chemistry (rok: 2016, tom: 408, strony: 1935-41), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-016-9308-3 - link to the publication
  11. N-Homocysteinylation impairs collagen cross-linking in cystathionine β-synthase-deficient mice: a novel mechanism of connective tissue abnormalities.
    Authors:
    Perła-Kajan J, Utyro O, Rusek M, Malinowska A, Sitkiewicz E, Jakubowski H
    Academic press:
    Faseb Journal (rok: 2016, tom: 30(11), strony: 3810-3821), Wydawca: FASEB
    Status:
    Published
    DOI:
    10.1096/fj.201600539 - link to the publication
  12. Homocysteine Modfication in Protein Structure/Function and Human Disease
    Authors:
    Hieronim Jakubowski
    Academic press:
    Physiological Reviews , Wydawca: American Physioological Society
    Status:
    Submitted
    DOI:
    brak - link to the publication
  13. Effects of endurance and endurance-strenght exercise on biochemical parameters of liver function in women with abdominal obesity
    Authors:
    D. Skrypnik, M.Ratajczak.,l. Karolkiewicz, E. Madry, D. Pupek- Musialik, R. Hansdorfer-Korzon, l.Walkowiak, H. Jakubowski, P. Bogdanski
    Academic press:
    Biomedicine & Pharmacotherapy (rok: 2016, tom: 80, strony: 45298), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.biopha.2016.02.017 - link to the publication
  14. Homocysteine Editing, Thioester Chemistry, Coenzyme A, and the Origin of Coded Peptide Synthesis
    Authors:
    Hieronim Jakubowski
    Academic press:
    Life (rok: 2017, tom: 7, strony: 45316), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/life7010006 - link to the publication
  15. Homocysteine-Thiolactone and N-Homocysteinylated Protein Induce Pro-atherogenic Changes in Gene Expression in Human Vascular Endothelial Cells
    Authors:
    Dorota Gurda, Luisa Handschuh, Weronika Kotkowiak, Hieronim Jakubowski
    Academic press:
    Amino Acids (rok: 2015, tom: 47 (7), strony: 1319-39), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00726-015-1956-7 - link to the publication
  1. Cystathionine ß-synthase deficiency induces changes in the mouse brain proteome associated with neurodegenerative disease
    Authors:
    Izabela Bielińska, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One Carbon Metabolism, Aarhus , Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18. 05
    Status:
    Published
    DOI:
    link to the publication
  2. Cystathionine-β synthase deficiency changes expression of major urinary proteins in mice
    Authors:
    Jacek Wróblewski, Joanna Perła-Kaján, Monika Wyszczelska-Rokiel, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  3. Histones are targeted for N-homocysteinylation in human endothelial cells
    Authors:
    Dorota Gurda, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  4. Homocysteine-thiolactone and cardiovascular disease in B-vitamin trials
    Authors:
    Borowczyk K, Głowacki R, Nygård O, Jakubowski H.
    Conference:
    10th Intl Conference on One Carbon Metabolism, Vitamins B and Homocysteine", Nancy, France (rok: 2015, ), Wydawca: Medical School, University of Lorraine
    Data:
    konferencja 7-11.07
    Status:
    Published
  5. Identification of specific N-homocysteinylated lysine residues in human plasma albumin and fibrinogen from cystathionine β-synthase-deficient patient
    Authors:
    Marta Sikora, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  6. Paraoxonase 1 protects against homocysteine-thiolactone accumulation in humans
    Authors:
    Perła-Kajan J, Borowczyk K, Głowacki R, Nygård O, Ueland PM, Jakubowski H.
    Conference:
    11th International Conference on Homocysteine and One-Carbon Metabolism, Aarhus, Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  7. Cystathionine β-synthase deficiency elevates N-homocysteinylation at specific lysine residues in fibrinogen and serum albumin in mice
    Authors:
    Marta Sikora, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2016" Wrocław 2016 (rok: 2016, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 13-16.09
    Status:
    Published
    DOI:
    link to the publication
  8. Identification of 2-pyridoxyl-1,3-thiazolidine-4-carboxylic acid, novel metabolite of vitamin B6 and cysteine in human plasma
    Authors:
    Rafał Głowacki, Monika Wyszczelska-Rokiel, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochimica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  9. N-homocysteinylation impairs collagen cross-linking in cystathionine β-synthasedeficient mice: a novel mechanism of connective tissue abnormalities
    Authors:
    Joanna Perla-Kajan, Marta Rusek, Agata Malinowska, Ewa Sitkiewicz, Olga Utyro, Hieronim Jakubowski
    Conference:
    2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2016" Wrocław 2016 (rok: 2016, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 13-16.09.
    Status:
    Published
    DOI:
    link to the publication
  10. Telomere Length Is Not Affected by the Cystathionine - β Synthase ( Cbs ) Genotype, Age, and Life Span In Mice
    Authors:
    Olga Utyro and Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One-Carbon Metabolism, Aarhus, Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  11. Tissue-specific effect of hyperhomocysteinemia on collagen and elastin cross-links in a cystathionine β-synthase-deficient mouse.
    Authors:
    Perła-Kajan J, Ogrodna K, Jakubowski H.
    Conference:
    14th Intl Congress on Amino Acids, Peptides and Proteins. Vienna, Austria (rok: 2015, ), Wydawca: Amino Acids 2016
    Data:
    konferencja 3-7.08
    Status:
    Published
  12. Inactivation of the bleomycin hydrolase (Blmh) gene affects the expression of glyoxalase domain-containing protein 4 (Glod4) gene in mice
    Authors:
    Olga Utyro, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochimica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  13. Label-free proteomic analysis of plasma of cystathionine β-synthase deficient mice and humans reveals changes in blood coagulation, hemostasis and antioxidant activity
    Authors:
    Marta Sikora,Izabela Bielińska ,Łukasz Marczak, Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One Carbon Metabolism, Aarhus , Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  14. Label-free proteomic analysis reveals changes in red-ox and lipid homeostasis in the liver of cystathionine β-synthase deficient mice
    Authors:
    Izabela Bielińska, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2016" Wrocław 2016 (rok: 2016, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 13-16.09
    Status:
    Published
    DOI:
    link to the publication
  15. N-homocysteinylation as a mechanism of connective tissue impairment in hyperhomocysteinemia due to cystathionine β-synthase deficiency
    Authors:
    Joanna Perła-Kajan, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  16. Properties of fibrin clots formed from plasma of cystathionine β-synthase-deficient patients and their unaffected family members
    Authors:
    Izabela Bielińska, Marta Sikora, Joanna Perła-Kajan, Michał Ząbczyk, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochimica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  17. Analysis of liver proteome in cystathionine beta-synthase deficient mice using 2D IEF/SDS-PAGE gel electrophoresis, MALDI-TOF mass spectrometry, and label-free based relative quantitative proteomics.
    Authors:
    Bielinska I, Marczak L, Jakubowski H.
    Conference:
    29th Annual Symposium of the Protein-Society, Barcelona (rok: 2015, ), Wydawca: Protein Science
    Data:
    konferencja 22-25.07
    Status:
    Published
  18. Correlations between sulfur-containing amino acids and albumin in human plasma
    Authors:
    Borowczyk K, Jakubowski H, Głowacki R.
    Conference:
    10th Intl Conference on One Carbon Metabolism, Vitamins B and Homocysteine, Nancy, France (rok: 2015, ), Wydawca: Medical School, University of Lorraine
    Data:
    konferencja 7-11.07
    Status:
    Published
  19. Identification of N-homocysteinylation sites in mouse plasma albumin and hemoglobin from cystathionine β-synthase deficient mice.
    Authors:
    Sikora M, Marczak L, Jakubowski H.
    Conference:
    10th Intl Conference on One Carbon Metabolism, Vitamins B and Homocysteine", Nancy, France (rok: 2015, ), Wydawca: Medical School, University of Lorraine
    Data:
    konferencja 7-11.07
    Status:
    Published
  20. Dietary hyperhomocysteinemia upregulates mTOR signaling and downregulates autophagy in the mouse brain
    Authors:
    Joanna Suszyńska-Zajczyk, Hieronim Jakubowski
    Conference:
    2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2016" Wrocław 2016 (rok: 2016, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 13-16.09
    Status:
    Published
    DOI:
    link to the publication
  21. Does hyperhomocysteinemia affect collagen and elastin cross-links in a mouse model of cystathionine β-synthase deficiency
    Authors:
    Perła-Kajan J, Jakubowski H.
    Conference:
    10th Intl Conference on One Carbon Metabolism, Vitamins B and Homocysteine", Nancy, France (rok: 2015, ), Wydawca: Medical School, University of Lorraine
    Data:
    konferencja 7-11.07
    Status:
    Published
  22. Hyperhomocysteinemia and bleomycin hydrolase modulate the expression of mouse brain proteins involved in neurodegeneration
    Authors:
    Joanna Suszyńska-Zajczyk, Magdalena Łuczak, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochimica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  23. Inactivation of the paraoxonase 1 gene affects the expression of mouse brain proteins involved in neurodegeneration
    Authors:
    Joanna Suszyńska-Zajczyk, Magdalena Łuczak, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochimica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  24. Methionine demethylation and protein damage: time - dependent accumulation of homocysteine in human hair keratin
    Authors:
    Kamila Borowczyk, Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One-Carbon Metabolism, Aarhus, Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  25. N - homocysteinylation impairs collagen cross - linking in cystathionine β -synthase -deficient mice: a novel mechanism of connective tissue abnormalities
    Authors:
    Joanna Perła - Kajan, Marta Rusek, Agata Malinowska, Ewa Sitkiewicz, Olga Utyro , and Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One-Carbon Metabolism, Aarhus, Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  26. Paraoxonase 1 (Pon1) regulates water homeostasis by controlling the expression of Fxr and Aqp2 proteins in mice.
    Authors:
    Wieloch M, Jakubowski H.
    Conference:
    29th Annual Symposium of the Protein-Society, Barcelona (rok: 2015, ), Wydawca: Protein Science
    Data:
    konferencja 22-25.07
    Status:
    Published

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