Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Multiparameter imaging of cells by using vibrational spectroscopy, AFM and SNOM: analysis of biochemical changes in single endothelium cells induced by stress, pathology and drug therapy

2013/08/A/ST4/00308

Keywords:

Raman IR imaging biochemical analysis chemometrics

Descriptors:

  • ST5_16:
  • ST4_3: Molecular architecture and structure
  • ST4_13: Solid-state chemistry, surface physicochemistry

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Jagielloński, Wydział Chemii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Małgorzata Barańska 

Number of co-investigators in the project: 9

Call: MAESTRO 4 - announced on 2012-12-15

Amount awarded: 2 224 400 PLN

Project start date (Y-m-d): 2013-09-17

Project end date (Y-m-d): 2017-09-16

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Lasery (2 szt.) (140 000 PLN)
  2. Objektyw imersyjny (20 000 PLN)
  3. Oprogramowanie chemometryczne i spektroskopowe, np. WitecPlus, The Unscrambler, Neurodeveloper, Origin (2 szt.) (13 000 PLN)
  4. Program do pomiarów i analizy danych AFM w trybie PFM i/lub DPFM (90 000 PLN)
  5. Przystawka pomiarowa do spektrometru.
  6. Urządzenie wielofunkcyjne (1 968 PLN)
  7. pH-metr.
  8. 3 komputery stacjonarne (3 szt.) (7 500 PLN)
  9. Elementy optyki do detektora FPA (20 970 PLN)
  10. Mieszadło mechaniczne (5 000 PLN)
  11. System oczyszczania wody Millipore z lampą UV (12 000 PLN)
  12. Miniwytrząsarka typu Vertex (1 500 PLN)
  13. elementy optyczne, elektroniczne i mechaniczne do spektrometrów (24 000 PLN)
  14. 3 laptopy (3 szt.) (10 388 PLN)
  15. Ramanowski spektrometr dyspersyjny ze wzbudzeniem w zakresie widzialnym/NIR (1 laser) (10 436 PLN)
  16. Wirówka mikrolitrowa z przyspieszeniem do 18 000 obrótów i regulacją temperatury (6 000 PLN)
  17. komputer stacjonarny z 2 monitorami o wysokiej mocy obliczeniowej (5 000 PLN)
  18. licencja oprogramowania office.

Information in the final report

  • Publication in academic press/journals (24)
  1. A new insight into the interaction of cisplatin with DNA: ROA spectroscopic studies on the therapeutic effect of the drug
    Authors:
    M. Gąsior-Głogowska, K. Malek ,G. Zajac, M. Baranska
    Academic press:
    Analyst (rok: 2016, tom: 141, strony: 291-296), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C5AN02140E - link to the publication
  2. Impact of cell cycle dynamics on pathology recognition: Raman imaging study
    Authors:
    K. Czamara, E. Karnas, Z. Majka, T. Wojcik, E. Zuba-Surma, M. Baranska, A. Kaczor
    Academic press:
    Journal of Biophotonics (rok: 2018, tom: e201800152, strony: 45301), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/jbio.201800152 - link to the publication
  3. Raman microscopy as a novel tool to detect endothelial dysfunction
    Authors:
    M. Baranska, A. Kaczor, K. Malek, A. Jaworska, K. Majzner, E. Staniszewska-Slezak, M.Z. Pacia, G. Zajac, J. Dybas, E. Wiercigroch
    Academic press:
    Pharmacological Reports (rok: 2015, tom: 67, strony: 736-743), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.pharep.2015.03.015 - link to the publication
  4. Raman spectroscopy as a sensitive probe of soft tissue composition – Imaging of cross-sections of various organs vs. single spectra of tissue homogenates
    Authors:
    J. Dybas, K. M. Marzec, M. Z. Pacia, K. Kochan, K. Czamara, K. Chrabaszcz, E. Staniszewska-Slezak, K. Malek, M. Baranska, A. Kaczor
    Academic press:
    Trends in Analytical Chemistry (rok: 2016, tom: 85, strony: 117-127), Wydawca: ELSEVIER
    Status:
    Published
    DOI:
    10.1016/j.trac.2016.08.014 - link to the publication
  5. A novel approach to investigate vascular wall in 3D: Combined Raman spectroscopy and atomic force microscopy for aorta en face imaging
    Authors:
    Marta Pilarczyk, b, Anna Rygula, Agnieszka Kaczora, Lukasz Mateuszuk, Edyta Maślak, Stefan Chlopicki, Małgorzata Barańska
    Academic press:
    Vibrational Spectroscopy (rok: 2014, tom: 75, strony: 39-44), Wydawca: ELSEVIER
    Status:
    Published
    DOI:
    10.1016/j.vibspec.2014.09.004 - link to the publication
  6. A possible Fourier transform infrared-based plasma fingerprint of angiotensin-converting enzyme inhibitor-induced reversal of endothelial dysfunction in diabetic mice
    Authors:
    E.Staniszewska-Slezak, E. Wiercigroch, A. Fedorowicz, E. Buczek, L.Mateuszuk, M. Baranska, S. Chlopicki, K. Malek
    Academic press:
    J. Biophotonics (rok: 2017, tom: e201700044., strony: 45302), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/jbio.201700044 - link to the publication
  7. Identification of a biochemical marker for endothelial dysfunction using Raman spectroscopy
    Authors:
    A. Rygula, M. Z. Pacia, L. Mateuszuk, A. Kaczor, R. B. Kostogrys, S. Chlopicki and M. Baranska
    Academic press:
    Analyst (rok: 2015, tom: 140, strony: 2185-2189), Wydawca: RSC
    Status:
    Published
    DOI:
    10.1039/C4AN01998A - link to the publication
  8. Resonance Raman in Vitro Detection and Differentiation of the Nitrite-Induced Hemoglobin Adducts in Functional Human Red Blood Cells
    Authors:
    K. M. Marzec, J. Dybas, S. Chlopicki, M. Baranska
    Academic press:
    The Journal of Physical Chemistry B (rok: 2016, tom: 120, strony: 12249-12260), Wydawca: ACS Publications
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.6b08359 - link to the publication
  9. Transmission versus transflection mode in FTIR analysis of blood plasma: is the electric field standing wave effect the only reason for observed spectral distortions?
    Authors:
    Staniszewska-Slezak , Rygula A, Malek K, Baranska M
    Academic press:
    Analyst (rok: 2015, tom: 140, strony: 2412-2421), Wydawca: RSC
    Status:
    Published
    DOI:
    10.1039/C4AN01842G - link to the publication
  10. Unsaturated lipid bodies as a hallmark of inflammation studied by Raman 2D and 3D microscopy
    Authors:
    K. Czamara, K. Majzner, A. Selmi, M. Baranska, Y. Ozaki, A. Kaczor
    Academic press:
    Scientific Reports (rok: 2017, tom: 7, strony: 45301), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/srep40889 - link to the publication
  11. Live endothelial cells imaged by Scanning Near-field Optical Microscopy (SNOM): capabilities and challenges
    Authors:
    K. Bulat, A. Rygula, E. Szafraniec, Y. Ozaki, M. Baranska
    Academic press:
    Journal of Biophotonics (rok: 2078, tom: 10, strony: 928-937), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/jbio.201600081 - link to the publication
  12. Nuclear accumulation of anthracyclines in the endothelium studied by bimodal imaging: fluorescence and Raman microscopy
    Authors:
    K. Majzner, T. Wojcik, E. Szafraniec, M. Lukawska, I. Oszczapowicz, S. Chlopicki and M. Baranska
    Academic press:
    Analyst (rok: 2015, tom: 140, strony: 2302-2310), Wydawca: RSC
    Status:
    Published
    DOI:
    10.1039/C4AN01882F - link to the publication
  13. Raman microscopy at the subcellular level: study on early apoptosis in endothelial cells induced by Fas ligand and cycloheximide
    Authors:
    K.Czamara, F. Petko, M. Baranska, A. Kaczor
    Academic press:
    Analyst (rok: 2016, tom: 141, strony: 1390-1397), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C5AN02202A - link to the publication
  14. Rhodamine 6G conjugated to gold nanoparticles as labels for both SERS and fluorescence studies on live endothelial cells
    Authors:
    Aleksandra Jaworska & Tomasz Wojcik & Kamilla Malek & Urszula Kwolek & Mariusz Kepczynski & Abu A. Ansary & Stefan Chlopicki & Malgorzata Baranska
    Academic press:
    Microchim Acta (rok: 2015, tom: 182, strony: 119-127), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00604-014-1307-5 - link to the publication
  15. Vascular diseases investigated ex vivo by using Raman, FT-IR and other methods
    Authors:
    K.M. Marzec, A. Rygula, M. Gasior-Glogowska, K. Kochan, K. Czamara, K. Bulat, K. Malek, A. Kaczor, M. Baranska
    Academic press:
    Pharmacological Reports (rok: 2015, tom: 67, strony: 744-750), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.pharep.2015.05.001 - link to the publication
  16. Raman microspectroscopy of human aortic valves: investigation of the local and global biochemical changes associated with calcification in aortic stenosis
    Authors:
    Krzysztof Czamara, Joanna Natorska, Przemysław Kapusta, Malgorzata Baranska and Agnieszka Kaczor
    Academic press:
    Analyst (rok: 2015, tom: 46, strony: 45402), Wydawca: RSC
    Status:
    Published
    DOI:
    10.1039/C4AN01856G - link to the publication
  17. 3D Raman imaging of systemic endothelial dysfunction in the murine model of metastatic breast cancer
    Authors:
    M. Z. Pacia, E. Buczek, A. Blazejczyk, A. Gregorius, J. Wietrzyk, S. Chlopicki, M. Baranska, A. Kaczor
    Academic press:
    Anal. Bioanal. Chem. (rok: 2016, tom: 408, strony: 3381–3387), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-016-9436-9 - link to the publication
  18. Biochemical changes of the endothelium in the murine model of NO-deficient hypertension
    Authors:
    M. Z. Pacia , L. Mateuszukb, S. Chlopicki , M. Baranska , A. Kaczor
    Academic press:
    Analyst (rok: 2015, tom: 140, strony: 2178-2184), Wydawca: RCS
    Status:
    Published
    DOI:
    10.1039/C4AN01870B - link to the publication
  19. Polypyridyl Substituted BODIPY Derivatives; Water Switchable Probes for Imaging Non-Aqueous Domains in Cells that Exhibit Halogen Substituent Dependent Localisation
    Authors:
    C. Dolan, A. Byrne, C. Long, K. Czamara, A. Kaczor, M. Baranska, T.E. Keyes
    Academic press:
    RSC Advances (rok: 2017, tom: 7, strony: 43743–43754), Wydawca: RSC
    Status:
    Published
    DOI:
    10.1039/c7ra07493j - link to the publication
  20. Raman spectroscopy of lipids: a review
    Authors:
    K. Czamara, K. Majzner, M. Z. Pacia, K. Kochan, A. Kaczor, M. Baranska
    Academic press:
    Journal of Raman Spectroscopy (rok: 2015, tom: 46, strony: 45402), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/jrs.4607 - link to the publication
  21. Spectroscopic studies of anthracyclines: Structural characterization and in vitro tracking
    Authors:
    E. Szafraniec, K. Majzner, Z. Farhane, H. J. Byrne, M. Lukawska, I. Oszczapowicz, S. Chlopicki, Malgorzata Baranska
    Academic press:
    Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (rok: 2016, tom: 169, strony: 152-160), Wydawca: ELSEVIER
    Status:
    Published
    DOI:
    10.1016/j.saa.2016.06.035 - link to the publication
  22. Complementary analysis of tissue homogenates composition obtained by Vis and NIR laser excitations and Raman spectroscopy
    Authors:
    E. Staniszewska-Slezak, K. Malek, M. Baranska
    Academic press:
    Spectrochimica Acta A (rok: 2015, tom: 147, strony: 245-256), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.saa.2015.03.086 - link to the publication
  23. Raman Optical Activity and Raman spectroscopy of carbohydrates in solution
    Authors:
    M. Dudek, G. Zajac, E. Szafraniec, E. Wiercigroch, S. Tott, K. Malek, A. Kaczor, M. Baranska
    Academic press:
    Spectrochimica Acta A (rok: 2019, tom: 206, strony: 597-612), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.saa.2018.08.017 - link to the publication
  24. Raman imaging providing insights into chemical composition of lipid droplets of different size and orgin: in hepatocytes and endothelium
    Authors:
    Katarzyna Majzner , Kamila Kochan , Neli Kachamakova-Trojanowska , Edyta Maslak , Stefan Chlopicki , Malgorzata Baranska
    Academic press:
    Analyticall chemistry (rok: 2014, tom: 86, strony: 6666-6674), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/ac501395g - link to the publication

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