Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Members of eIF4E protein family, their affinities towards 5' mRNA cap and protein binding partners: an interdisciplinary approach to determine structural and functional correlations

2012/07/B/NZ1/00118

Keywords:

eukaryotic initiation translation factor eIF4E 4E-binding proteins translation initiation 5' mRNA cap cap-4 capped oligonucleotide trypanosomatides nematodes fluorescence titration TST isothermal titration calorimetry ITC circular dichroism CD stopped-flow thermodynamic parameters molecular modeling

Descriptors:

  • NZ1_3: Biophysics
  • NZ1_4: Structural biology
  • NZ1_6: Synthetic biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Warszawski, Wydział Fizyki

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Edward Darżynkiewicz 

Number of co-investigators in the project: 11

Call: OPUS 4 - announced on 2012-09-15

Amount awarded: 995 000 PLN

Project start date (Y-m-d): 2013-07-04

Project end date (Y-m-d): 2017-07-03

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Chromatograf cieczowy AKTA Purifier z szafa chłodniczą (176 000 PLN)
  2. Inkubator z wytrząsaniem.

Information in the final report

  • Publication in academic press/journals (8)
  • Articles in post-conference publications (1)
  • Book publications / chapters in book publications (1)
  1. Five eIF4E isoforms from Arabidopsis thaliana are characterized by distinct features of cap analogs binding
    Authors:
    Kropiwnicka A, Kuchta K, Lukaszewicz M, Kowalska J, Jemielity J, Ginalski K, Darzynkiewicz E, Zuberek J
    Academic press:
    Biochemical and Biophysical Research Communications (rok: 2015, tom: 456, strony: 47-52), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbrc.2014.11.032. - link to the publication
  2. Phosphate-modified analogues of m(7)GTP and m(7)Gppppm(7)G-Synthesis and biochemical properties
    Authors:
    Ziemniak M., Kowalska J., Lukaszewicz M., Zuberek J., Wnek K., Darzynkiewcz E, Jemielity J
    Academic press:
    Bioorganic & Medicinal Chemistry (rok: 2015, tom: 23, strony: 5369-5381), Wydawca: ELSEVIER
    Status:
    Published
    DOI:
    10.1016/j.bmc.2015.07.052 - link to the publication
  3. Tryptophan Residues from Cap Binding Slot in eIF4E Family Members:Their Contributions to Near-UV Circular Dichroism Spectra
    Authors:
    Zuberek J., Stelmachowska A.
    Academic press:
    Journal Physical Chemistry and Biophysics (rok: 2017, tom: 7, strony: 250), Wydawca: OMICS International
    Status:
    Published
    DOI:
    10.4172/2161-0398.1000250 - link to the publication
  4. Distinct Features of Cap Binding by eIF4E1b Proteins
    Authors:
    Kubacka D, Miguel RN, Minshall N, Darzynkiewicz E, Standart N, Zuberek J
    Academic press:
    Journal Molecular of Biology (rok: 2015, tom: 427, strony: 387-405), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jmb.2014.11.009 - link to the publication
  5. Trypanosoma brucei translation initiation factor homolog EIF4E6 forms a tripartite cytosolic complex with EIF4G5 and a capping enzyme homolog
    Authors:
    Freire ER, Malvezzi AM, Vashisht AA, Zuberek J, Saada EA, Langousis G, Nascimento JD, Moura D, Darzynkiewicz E, Hill K, de Melo Neto OP, Wohlschlegel JA, Sturm NR, Campbell DA
    Academic press:
    Eukaryotic Cell (rok: 2014, tom: 13, strony: 896-908), Wydawca: American Society for Microbiology
    Status:
    Published
    DOI:
    10.1128/EC.00071-14 - link to the publication
  6. Diverse cap-binding properties of Drosophila eIF4E isoforms
    Authors:
    Zuberek J., Kuchta K., Hernandez G., Sonenberg N., Ginalski K.
    Academic press:
    Biochimica et Biophysica Acta, Proteins and Proteomics (rok: 2016, tom: 1864, strony: 1292-1303), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbapap.2016.06.015 - link to the publication
  7. Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes
    Authors:
    Kowalska J, Wypijewska del Nogal A, Darzynkiewicz ZM, Buck J, Nicola C, Kuhn AN, Lukaszewicz M, Zuberek J, Strenkowska M, Ziemniak M, Maciejczyk M, Bojarska E, Rhoads RE, Darzynkiewicz E, Sahin U, Jemielity J
    Academic press:
    Nuclei Acids Research (rok: 2014, tom: 42, strony: 10245-10264), Wydawca: OXFORD University Press
    Status:
    Published
    DOI:
    10.1093/nar/gku757 - link to the publication
  8. eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei
    Authors:
    Freire ER, Vashisht AA, Malvezzi AM, Zuberek J, Langousis G, Saada EA, Nascimento Jde F, Stepinski J, Darzynkiewicz E, Hill K, De Melo Neto OP, Wohlschlegel JA, Sturm NR, Campbell DA
    Academic press:
    RNA (rok: 2014, tom: 20, strony: 1272-1286), Wydawca: RNA Society
    Status:
    Published
    DOI:
    10.1261/rna.045534.114 - link to the publication
  1. 5'cap-binding site mutations in eIF4E abolish positive effect of the cap
    Authors:
    Modrak-Wojcik A., Zuberek J., Stelmachowska A., Zdanowski K., Stolarski R.,
    Conference:
    XVI Conference of the Polish Biophysical Society (rok: 2016, ), Wydawca: Uniwersytet im. Adama Mickiewicza
    Data:
    konferencja 2016-06-28 - 2016-07-01
    Status:
    Published
  1. mRNA and snRNA Cap Analogs: Synthesis and Applications
    Authors:
    Stepinski J., Darzynkiewicz E.
    Book:
    Chemical Biology of Nucleic Acids. Fundamentals and Clinical Applications (rok: 2014, tom: brak, strony: 511-561), Wydawca: Springer Heidelberg New York Dordrecht London
    Status:
    Published
    DOI:
    link to the publication

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