Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

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Neurotoxic oligomers of human cystatin C - identification and structural studies

2012/06/M/ST4/00036

Keywords:

human cystatin C structural studies neurotoxic oligomers TEM AFM SAXS SANS

Descriptors:

  • ST4_4: Theoretical and computational chemistry
  • ST4_13: Solid-state chemistry, surface physicochemistry
  • ST4_3: Molecular architecture and structure

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet im. Adama Mickiewicza w Poznaniu, Wydział Fizyki

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Maciej Kozak 

Number of co-investigators in the project: 9

Call: HARMONIA 3 - announced on 2012-06-15

Amount awarded: 630 500 PLN

Project start date (Y-m-d): 2013-04-09

Project end date (Y-m-d): 2017-04-08

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Information in the final report

  • Publication in academic press/journals (8)
  • Articles in post-conference publications (25)
  1. Bioengineering the spider silk sequence to modify its affinity for drugs
    Authors:
    Kamil Kucharczyk, Marek Weiss, Katarzyna Jastrzebska, Magdalena Luczak, Arkadiusz Ptak, Maciej Kozak, Andrzej Mackiewicz, Hanna Dams-Kozlowska
    Academic press:
    International Journal of Nanomedicine (rok: 2018, tom: 13, strony: 4247-4261), Wydawca: Dovepress
    Status:
    Published
    DOI:
    10.2147/IJN.S168081 - link to the publication
  2. Effect of calcium ions on structure and stability of the C1q-like domain of otolin-1 from human and zebrafish
    Authors:
    Rafał Hołubowicz, Magdalena Wojtas, Michał Taube, Maciej Kozak, Andrzej Ożyhar, Piotr Dobryszycki
    Academic press:
    The FEBS Journal (rok: 2017, tom: 284, strony: 4278–4297), Wydawca: Federation of European Biochemical Societies
    Status:
    Published
    DOI:
    10.1111/febs.14308 - link to the publication
  3. The method of purifying bioengineered spider silk determines the secondary structure of soluble silk and silk sphere properties
    Authors:
    Katarzyna Jastrzebska, Edyta Felcyn, Maciej Kozak, Miroslaw Szybowicz, Tomasz Buchwald, Zuzanna Pietralik, Teofil Jesionowski, Andrzej Mackiewicz, Hanna Dams- Kozlowska
    Academic press:
    Scientific Reports (rok: 2016, tom: 6, strony: 28106), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/srep28106 - link to the publication
  4. Structural characterization of covalently stabilized human cystatin C oligomers
    Authors:
    M. Chrabąszczewska, S. Rodziewicz-Motowidło, A. Grubb, C.M. Dobson, J.R. Kumita and M. Kozak
    Academic press:
    Biophysical Journal (rok: 2019, tom: -, strony: 45314), Wydawca: Cell Press
    Status:
    Submitted
    DOI:
    brak - link to the publication
  5. The domain swapping of human cystatin C induced by synchrotron radiation
    Authors:
    Michał Taube, Zuzanna Pietralik, Aneta Szymańnska, Kosma Szutkowski, Daniel Clemens, Anders Grubb and Maciej Kozak
    Academic press:
    Scientific Reports (rok: 2019, tom: -, strony: 12420), Wydawca: Nature Publishing Group
    Status:
    Accepted for publication
    DOI:
    10.1038/s41598-019-44811-1 - link to the publication
  6. Cyclic trimer of human cystatin C, an amyloidogenic protein - molecular dynamics and experimental studies
    Authors:
    Magdalena Murawska, Martyna Maszota-Zieleniak, Zuzanna Pietralik, Michał Taube, Sylwia Rodziewicz-Motowidło, Aneta Szymańska, Kosma Szutkowski, Daniel Clemens, Anders Grubb, Maciej Kozak
    Academic press:
    Journal of Applied Physics (rok: 2018, tom: 123, strony: 174701 (pages 1-10)), Wydawca: American Institute of Physics
    Status:
    Published
    DOI:
    10.1063/1.5023807 - link to the publication
  7. Overall conformation of covalently stabilized domain-swapped dimer of human cystatin C in solution
    Authors:
    Magdalena Murawska, Aneta Szymańska, Anders Grubb, Maciej Kozak
    Academic press:
    Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms (rok: 2017, tom: 411, strony: 136-140), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.nimb.2017.02.090 - link to the publication
  8. Stabilization, Characterization and Selective Removal of Cystatin C Amyloid Oligomers
    Authors:
    Gustav Ranheimer Östner, Veronica Lindström, Per Hjort Christensen, Maciej Kozak, Magnus Abrahamson and Anders Grubb
    Academic press:
    The Journal of Biological Chemistry (rok: 2013, tom: 288, strony: 16438-16450), Wydawca: American Society of Biochemistry and Molecular Biology
    Status:
    Published
    DOI:
    10.1074/jbc.M113.469593 - link to the publication
  1. Molecular dynamics simulation studies of HCC oligomeric forms
    Authors:
    Magdalena Murawska, Anders Grubb, Martyna Maszota, Sylwia Rodziewicz- Motowidło, Maciej Kozak
    Conference:
    International Meeting CYSTATINS 2014, (rok: 2014, ), Wydawca: Fundacja Centrum Transferu Technologii Synergy
    Data:
    konferencja 12-14.06.2014
    Status:
    Published
  2. Proces oligomeryzacji i jego znaczenie w chorobach neurodegeneracyjnych na przykładzie ludzkiej cystatyny C
    Authors:
    Zuzanna Pietralik, Aneta Szymańska, Janet R. Kumita, Christopher M. Dobson, Maciej Kozak
    Conference:
    Konferencja Polskiego Towarzystwa Fizyki Medycznej (rok: 2017, ), Wydawca: PTFM
    Data:
    konferencja 1-3 czerwca
    Status:
    Published
  3. SAXS studies of selected flexible proteins or proteins of modular structure
    Authors:
    Maciej Kozak, Zuzanna Pietralik, Michał Taube
    Conference:
    KSUPS 2015 (rok: 2015, ), Wydawca: PTPS - Synchrotron Radiation in Natural Science Vol. 14, No. 1 – 2 (2015)
    Data:
    konferencja 1-4/09/2015
    Status:
    Published
  4. Time-resolved SAXS studies of human cystatin C – first observation of radiation induced domain swapping
    Authors:
    Maciej Kozak, Michał Taube, Magdalena Murawska, Aneta Szymanska, Anders Grubb
    Conference:
    International Meeting CYSTATINS 2014 (rok: 2014, ), Wydawca: Fundacja Centrum Transferu Technologii Synergy
    Data:
    konferencja 12-14.06.2014
    Status:
    Published
  5. Circular dichroism studies of possible changes in protein secondary structure induced by uranyl formate
    Authors:
    Julia Ludwiczak, Maciej Gielnik, Maciej Kozak
    Conference:
    50th Zakopane School of Physics (rok: 2015, ), Wydawca: IFJ PAN
    Data:
    konferencja 18-23/05/2015
    Status:
    Published
  6. Human cystatin C oligomers and radiation induced domain swapping
    Authors:
    Maciej Kozak, Michał Taube, Aneta Szymańska, Anders Grubb
    Conference:
    Protein Folding Conference 2014 (rok: 2014, ), Wydawca: Zing conferences
    Data:
    konferencja 16-19/07/2014
    Status:
    Published
  7. Molecular dynamic of donut-like form of human cystatin C in solution
    Authors:
    Magdalena Murawska, Andres Grubb, Sylwia Rodziewicz-Motowidło, Marta Maszota, Maciej Kozak.
    Conference:
    XV Zjazd Polskiego Towarzystwa Biofizycznego, (rok: 2013, ), Wydawca: PTBiofiz
    Data:
    konferencja Nałęczów 26-28 czerwca 2013,
    Status:
    Published
  8. Molecular dynamics simulation studies of human cystatin C oligomerization
    Authors:
    Magdalena Murawska, Sylwia Rodziewicz Motowidło, Maciej Kozak
    Conference:
    BIO Congress Warsaw, Poland September 9th–12th 2014 (rok: 2014, ), Wydawca: Polish Biochemical Society and of the Committee of Biochemistry and Biophysics Polish Academy of Sciences
    Data:
    konferencja 9-12/09/2014
    Status:
    Published
    DOI:
    link to the publication
  9. Oligomerization and structural characterization of native human cystatin C and its single-point mutants
    Authors:
    Zuzanna Pietralik, Aneta Szymanska, Janet R. Kumita, Christopher M. Dobson, Maciej Kozak
    Conference:
    13th International School and Symposium on Synchrotron Radiation in Natural Science (rok: 2016, ), Wydawca: PTPS - Synchrotron Radiation in Natural Science Vol. 15, No. 1 – 2 (2016)
    Data:
    konferencja 13 - 18/06/2016
    Status:
    Published
  10. Radiation damages in BioSAXS - comparison of changes induced by synchrotron radiation and lab source
    Authors:
    Maciej Kozak, Michał Taube, Zuzanna Pietralik, Maciej Gielnik, Aneta Szymańska, Igor Zhukov
    Conference:
    10th International Conference on X-Ray Investigations of Polymer Structure (rok: 2016, ), Wydawca: ATH
    Data:
    konferencja 6 - 9/12/2016
    Status:
    Published
  11. Self-association of HCC oligomers - AFM studies
    Authors:
    Zuzanna Pietralik, Janet R. Kumita, Christopher M. Dobson, Anders Grubb and Maciej Kozak
    Conference:
    Cystatins 2016 (rok: 2016, ), Wydawca: Lund University
    Data:
    konferencja 2 - 4/06/2016
    Status:
    Published
  12. Spectroscopic characterization of human cystatin C and its mutants
    Authors:
    Zuzanna Pieralik, Aneta Szymańska, Maciej Kozak
    Conference:
    KSUPS 2015 (rok: 2015, ), Wydawca: PTPS - Synchrotron Radiation in Natural Science Vol. 14, No. 1 – 2 (2015)
    Data:
    konferencja 1-4/09/2015
    Status:
    Published
  13. Oligomeric states of Human Cystatin C variants – atomic force microscopy and spectroscopic studies
    Authors:
    Zuzanna Pietralik, Żaneta Kołodziejska, Aneta Szymanska, Janet R. Kumita, Christopher M. Dobson, Maciej Kozak
    Conference:
    Alzheimer's disease and neurodegenerative disorders: what challenges for tomorrow (rok: 2016, ), Wydawca: Folia Neuropathologica 54/4
    Data:
    konferencja 42471
    Status:
    Published
  14. Spectroscopic and SAXS Studies of Human Cystatin C Mutants - Early Stages of Amyloid Formation Process
    Authors:
    Maciej Kozak, Zuzanna Pietralik, Aneta Szymanska, Michał Taube
    Conference:
    59BPS Meeting (rok: 2015, ), Wydawca: Biophysical Society
    Data:
    konferencja 7-11/02/2015
    Status:
    Published
    DOI:
    link to the publication
  15. Structural Studies of the Oligomerization Process of Human Cystatin C Variants
    Authors:
    Zuzanna Pietralik, Magdalena Murawska, Aneta Szymanska, Janet R. Kumita, Christopher M. Dobson, Maciej Kozak
    Conference:
    60th Annual Meeting Biophysical Society (rok: 2016, ), Wydawca: Biophysical Society. Published by Elsevier Inc.
    Data:
    konferencja 27/02-2/03/2016
    Status:
    Published
    DOI:
    link to the publication
  16. The order-disorder processes in solution SAXS studies of the protein folding
    Authors:
    Maciej Kozak, Michał Taube, Zuzanna Pietralik, Fabian Giska, Magdalena Krzymowska, Jacek Hennig, Andrzej Ożyhar, Aneta Szymańska
    Conference:
    Second Polish-Korean Conference on Protein Folding Theoretical and Experimental Approaches (rok: 2016, ), Wydawca: Uniwersytet Gdański
    Data:
    konferencja 28/05 – 01/06/2016
    Status:
    Published
  17. BioSAXS – a tool for structural studies of complex or disordered biomacromolecules
    Authors:
    Maciej Kozak, Michał Taube, Zuzanna Pietralik, Maciej Gielnik, Julia Ludwiczak, Aneta Szymańska, Igor Zhukov
    Conference:
    Bio2016 – Expanding beyond the limit - 2nd Congress of Polish Biochemistry, Cell Biology, Biotechnology and Bioinformatics (rok: 2016, ), Wydawca: Polskie Towarzystwo Biochemiczne - Acta Biochimica Polonica, Supplement 2, 2016
    Data:
    konferencja 13 - 16/09/2016
    Status:
    Published
  18. Interactions of synchrotron radiation with biological samples - radiation induced domain swapping
    Authors:
    Maciej Kozak, Michał Taube, Zuzanna Pietralik, Anders Grubb, Aneta Szymańska
    Conference:
    50th Zakopane School of Physics (rok: 2015, ), Wydawca: IFJ PAN
    Data:
    konferencja 18-23/05/2015
    Status:
    Published
  19. Methylamine vanadate can change overall secondary structure of proteins
    Authors:
    Maciej Gielnik, Julia Ludwiczak, Maciej Kozak
    Conference:
    50th Zakopane School of Physics (rok: 2015, ), Wydawca: IFJ PAN
    Data:
    konferencja 18-23/05/2015
    Status:
    Published
  20. Otrzymywanie oraz charakterystyka oligomerów ludzkiej cystatyny C oraz jej punktowych mutantów
    Authors:
    Zuzanna Pietralik, Aneta Szymańska, Janet R. Kumita, Christopher M. Dobson
    Conference:
    IX Seminarium STM/AFM 2016 (rok: 2016, ), Wydawca: Instytut Fizyki, Uniwersytet Jagielloński
    Data:
    konferencja 30/11 - 4/12/2016
    Status:
    Published
  21. Characterization of human cystain C variants - structural and spectroscopic studies
    Authors:
    Zuzanna Pietralik, Michał Taube, Aneta Szymańska, Janet R. Kumita, Christopher M. Dobson, Maciej Kozak
    Conference:
    10th International Conference on X-Ray Investigations of Polymer Structure (rok: 2016, ), Wydawca: ATH
    Data:
    konferencja 6 - 9/12/2016
    Status:
    Published
  22. Conformational changes of peptides based on human Cystatin C steric zipper region in the presence of gemini surfactants
    Authors:
    Weronika Andrzejewska, Julia Ludwiczak, Emilia Iłowska, Aneta Szymańska and Maciej Kozak
    Conference:
    13th International School and Symposium on Synchrotron Radiation in Natural Science (rok: 2016, ), Wydawca: PTPS - Synchrotron Radiation in Natural Science Vol. 15, No. 1 – 2 (2016)
    Data:
    konferencja 13 - 18/06/2016
    Status:
    Published
  23. Oligomerization process of HCC and its mutants - electrophoretic and fluorescent studies
    Authors:
    Zuzanna Pietralik, Aneta Szymanska, Janet R. Kumita, Christopher M. Dobson and Maciej Kozak
    Conference:
    Cystatins 2016 (rok: 2016, ), Wydawca: Lund University
    Data:
    konferencja 2 - 4/06/2016
    Status:
    Published
  24. Small angle X-ray scattering (SAXS) studies of human cystatin C in solution
    Authors:
    Magdalena Murawska, Anders Grubb, Maciej Kozak.
    Conference:
    XV Zjazd Polskiego Towarzystwa Biofizycznego, (rok: 2013, ), Wydawca: PTBiofiz
    Data:
    konferencja Nałęczów 26-28 czerwca 2013,
    Status:
    Published
  25. Structure of HCC oligomers – SAXS and MD studies
    Authors:
    Magdalena Murawska, Anders Grubb, Martyna Maszota, Sylwia Rodziewicz-Motowidło, Maciej Kozak
    Conference:
    KSUPS2012 (rok: 2013, ), Wydawca: PTPS - Synchrotron Radiation in Natural Science Vol. 12, No. 1 – 2 (2013)
    Data:
    konferencja 9-11/09/2013
    Status:
    Published
    DOI:
    link to the publication

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