Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Molecular mechanisms behind functional diversification of mitochondrial Hsp70 chaperones

2012/06/A/NZ1/00002

Keywords:

Hsp70 J-domain proteins iron sulfur clusters mitochondria yeast mitochondrial DNA

Descriptors:

  • NZ1_1: Molecular biology
  • NZ1_2: Biochemistry
  • NZ1_4: Structural biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Gdański, Międzyuczelniany Wydział Biotechnologii Uniwersytetu Gdańskiego i Gdańskiego Uniwersytetu Medycznego

woj. pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jarosław Marszałek 

Number of co-investigators in the project: 7

Call: MAESTRO 3 - announced on 2012-06-15

Amount awarded: 2 653 260 PLN

Project start date (Y-m-d): 2013-03-26

Project end date (Y-m-d): 2019-03-25

Project duration:: 72 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Komputerowa stacja robocza.
  2. Pozostałe wyposażenie komory beztlenowej: Wirówka z chłodzeniem, Mikser, Inkubator do hodowli mikroorganizmów, Termo-mikser (45 000 PLN)
  3. monitory wysokiej rozdzielczości.
  4. Spektrofluorymetr (165 000 PLN)
  5. System chromatografii niskociśnieniowej do czyszczenia białek w warunkach beztlenowych. (60 000 PLN)
  6. komputer osobisty pracujący w systemie OS i Windows.
  7. Komora prasy French'a (30 000 PLN)
  8. Rotor Assembly (44 999 PLN)
  9. termomikser.

Information in the final report

  • Publication in academic press/journals (8)
  • Book publications / chapters in book publications (1)
  1. Structure and evolution of 1 the 4-helix bundle domain of Zuotin, 2 a J-domain protein co-chaperone of Hsp70
    Authors:
    Om Kumar Shrestha, Ruchika Sharma, Bartlomiej Tomiczek, Woonghee Lee, Marco Tonelli, Gabriel Cornilescu, Milena Stolarska, Lukasz Nierzwicki, Jacek Czub, John L. Markley, Jaroslaw Marszalek, Szymon J. Ciesielski, Elizabeth A. Craig
    Academic press:
    PlosOne (rok: 2019, tom: 14(5), strony: 45312), Wydawca: Public Library of Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0217098 - link to the publication
  2. Function, evolution, and structure of J-domain proteins
    Authors:
    Kampinga, HH; Andreasson, C; Barducci, A; Cheetham, ME; Cyr, D; Emanuelsson, C; Genevaux, P; Gestwicki, JE; Goloubinoff, P; Huerta-Cepas, J; Kirstein, J; Liberek, K; Mayer, MP; Nagata, K; Nillegoda, NB; Pulido, P; Ramos, C; De Los Rios, P; Rospert, S; Rosenzweig, R; Sahi, C; Taipale, M; Tomiczek, B; Ushioda, R; Young, JC; Zimmermann, R; Zylicz, A; Zylicz, M; Craig, EA; Marszalek, J
    Academic press:
    CELL STRESS & CHAPERONES (rok: 2019, tom: 24, strony: 45488), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s12192-018-0948-4 - link to the publication
  3. Protection of scaffold protein Isu from degradation by the Lon protease Pim1 as a component of Fe–S cluster biogenesis regulation
    Authors:
    Szymon J. Ciesielski, Brenda Schilke, Jaroslaw Marszalek, and Elizabeth A. Craig
    Academic press:
    Molecular Biology of the Cell (rok: 2016, tom: 27(7), strony: 1060-1068), Wydawca: The American Society for cell Biology
    Status:
    Published
    DOI:
    10.1093/molbev/msv254 - link to the publication
  4. Fe–S Cluster Hsp70 Chaperones: The ATPase Cycle and Protein Interactions
    Authors:
    Rafal Dutkiewicz, Malgorzata Nowak, Elizabeth A. Craig, Jaroslaw Marszalek
    Academic press:
    Methods in Enzymology (rok: 2017, tom: Volume 595, strony: 161-184), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/bs.mie.2017.07.004 - link to the publication
  5. How Do J-Proteins Get Hsp70 to Do So Many Different Things?
    Authors:
    Elizabeth A. Craig, Jaroslaw Marszalek
    Academic press:
    Trends in Biochemical Sciences (rok: 2017, tom: Vol. 42, No. 5, strony: 355-368), Wydawca: Esevier
    Status:
    Published
    DOI:
    10.1016/j.tibs.2017.02.007 - link to the publication
  6. Nucleoid localization of Hsp40 Mdj1 is important for its function in maintenance of mitochondrial DNA.
    Authors:
    Grzegorz L. Ciesielski, Magdalena Plotka, Mateusz Manicki, Brenda A. Schilke, Rafał Dutkiewicz, Chandan Sahi, Jarosław Marszalek, Elizabeth A. Craig
    Academic press:
    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (rok: 2013, tom: 1833, strony: 2233 - 2243), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j/bbamcr.2013.05.012 - link to the publication
  7. Overlapping binding sites of frataxin homologue assembly factor and the heat shock protein 70 transfer factor on the Isu iron-sulfur cluster scaffold protein
    Authors:
    Mateusz Manicki, Julia Majewska, Szymon Ciesielski, Branda Schilke, Anna Blenska, Jacek Kominek, Jarosław Marszałek, Elizabeth A. Craig, and Rafał Dutkiewicz
    Academic press:
    The Journal of Biological Chemistry (rok: 2014, tom: vol. 289 no 40, strony: 30268-30278), Wydawca: The American Society of Biochemistry and Molecular Biology, Inc.
    Status:
    Published
    DOI:
    10.1074/jbc.M114.596726 - link to the publication
  8. Iron–Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein–Protein Interaction
    Authors:
    Wojciech Delewski, Bogumiła Paterkiewicz, Mateusz Manicki,Brenda Schilke, Bartłomiej Tomiczek, Szymon J. Ciesielski, Łukasz Nierzwicki, Jacek Czub, Rafal Dutkiewicz, Elizabeth A. Craig, and Jaroslaw Marszałek
    Academic press:
    Molecular Biology and Evolution (rok: 2015, tom: 33(3), strony: 643-656), Wydawca: Molecular Biology and Evolution
    Status:
    Published
    DOI:
    10.1093/molbev/msv254 - link to the publication
  1. Yeast Hsp70 and J-protein Chaperones: Function and Interaction Network
    Authors:
    Elizabeth A. Craig and Jaroslaw Marszalek
    Book:
    The Molecular Chaperones Interaction Networks in Protein Folding and Degradation (rok: 2014, tom: 3, strony: 53-82), Wydawca: Springer Science+Business Media New York 2014
    Status:
    Published
    DOI:
    link to the publication

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