Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Identification of Erwinia amylovora genes induced during infection and disease (fire blight) developement process

2012/05/B/NZ9/03455

Keywords:

bacteria apple RNA gene expression NextGen sequencing pyrosequencing transcriptomics virulence factors pathogenicity

Descriptors:

  • NZ9_4: Horticulture

Panel:

NZ9 - Fundamentals of applied life sciences and biotechnology: agricultural, forestry, horticulture, animal production and fishery, food and nutrition sciences, industrial biosciences, environmental biotechnology and remediation

Host institution :

Instytut Ogrodnictwa

woj. łódzkie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Joanna Puławska 

Number of co-investigators in the project: 6

Call: OPUS 3 - announced on 2012-03-15

Amount awarded: 570 534 PLN

Project start date (Y-m-d): 2013-02-21

Project end date (Y-m-d): 2017-02-20

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. rotor do wirówki SIGMA 3K30 (5 400 PLN)

Information in the final report

  • Publication in academic press/journals (3)
  1. Validation of reference genes for the normalization of the RT-qPCR gene expression of virulence genes of Erwinia amylovora in apple shoots
    Authors:
    Monika Kałużna , Anita Kuras & Joanna Puławska
    Academic press:
    Scientific Reports (rok: 2017, tom: 1,70416666666667, strony: 45300), Wydawca: NATURE PUBLISHING GROUP
    Status:
    Published
    DOI:
    10.1038/s41598-017-02078-4 - link to the publication
  2. Evaluation of different RNA extraction methods for high-quality total RNA and mRNA from Erwinia amylovora in planta
    Authors:
    Monika Kałużna & Anita Kuras & Artur Mikiciński & Joanna Puławska
    Academic press:
    European Journal of Plant Pathology (rok: 2016, tom: 146, strony: 893-899), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10658-016-0967-x - link to the publication
  3. Comparative transcriptome analysis of a lowly virulent strain of Erwinia amylovora in shoots of two apple cultivars – susceptible and resistant to fire blight
    Authors:
    Joanna Puławska, Monika Kałużna, Wojciech Warabieda, Artur Mikiciński
    Academic press:
    BMC Genomics (rok: 2017, tom: 1,35277777777778, strony: 45306), Wydawca: BioMed Central Ltd
    Status:
    Published
    DOI:
    10.1186/s12864-017-4251-z - link to the publication

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