Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Influence of nucleotide sequence on the yield of radio- and photodamage to double stranded DNA fragments labelled with the halogen derivatives of nucleobases

2012/05/B/ST5/00368

Keywords:

radio- and photodamage to DNA anions of halogenated nucleobases long-range electron transfer DHPLC MS spectrometry real-time PCR

Descriptors:

  • ST5_11: Smart materials – self assembled materials, external stimuli-responsive materials
  • ST5_16:
  • ST5_19:

Panel:

ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution :

Uniwersytet Gdański, Wydział Chemii

woj. pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Janusz Rak 

Number of co-investigators in the project: 6

Call: OPUS 3 - announced on 2012-03-15

Amount awarded: 597 400 PLN

Project start date (Y-m-d): 2013-03-05

Project end date (Y-m-d): 2016-03-04

Project duration:: 36 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Termocykler do real-time PCR-u wraz z oprogramowaniem do analizy temperatur topnienia (Biorad CFX96 plus program HRM – www.biorad.com wraz z oprogramowaniem Precision Melt Analysis Software) (175 000 PLN)
  2. Modernizacja stanowiska do naświetlań UV (46 999 PLN)
  3. Wirówka (Spectrafuge Mini, Labnet International).
  4. Wysokosprawny chromatograf cieczowy firmy Shimadzu (używany).
  5. Zestaw do elektroforezy pozioomej (Bio-Rad Polska Sp. z o.o).

Information in the final report

  • Publication in academic press/journals (8)
  1. Quantitative assay of photoinduced DNA strand breaks by real-time PCR
    Authors:
    Justyna Wiczk, Kinga Westphal, Janusz Rak
    Academic press:
    Journal of Pharmaceutical and Biomedical Analysis (rok: 2016, tom: 128, strony: 1480–484), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jpba.2016.06.023 - link to the publication
  2. Reactivity Pattern of Bromonucleosides Induced by 2-Hydroxypropyl Radicals. Photo-, Radiation-chemical and Computational Studies
    Authors:
    Magdalena Zdrowowicz, Lidia Chomicz, Justyna Miloch, Justyna Wiczk, Janusz Rak, Gabriel Kciuk, Krzysztof Bobrowski
    Academic press:
    The Journal of Physical Chemistry B (rok: 2015, tom: 119, strony: 6545−6554), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.5b01904 - link to the publication
  3. UV-Induced Strand Breaks in Double-Stranded DNA Labeled with 5-Bromouracil: Frank or Secondary?
    Authors:
    Justyna Wiczk, Justyna Miloch, Janusz Rak
    Academic press:
    The Journal of Physical Chemistry Letters (rok: 2013, tom: 4, strony: 4014-4018), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/jz402208z - link to the publication
  4. 5-Bromo-2'-deoxycytidine — a Potential DNA Photosensitizer
    Authors:
    Magdalena Zdrowowicz, Paweł Wityk, Barbara Michalska, Janusz Rak
    Academic press:
    Organic & Biomolecular Chemistry (rok: 2016, tom: 14, strony: 9312-9321), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C6OB01446A - link to the publication
  5. Irreversible electron attachment – a key to DNA damage by solvated electrons in aqueous solution
    Authors:
    Kinga Westphal, Justyna Wiczk, Justyna Miloch, Gabriel Kciuk, Krzysztof Bobrowski, Janusz Rak
    Academic press:
    Organic & Biomolecular Chemistry (rok: 2015, tom: 13, strony: 10362–10369), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c5ob01542a - link to the publication
  6. Mechanisms of Damage to DNA Labeled with Electrophilic Nucleobases Induced by Ionizing or UV Radiation
    Authors:
    Janusz Rak, Lidia Chomicz, Justyna Wiczk, Kinga Westphal, Magdalena Zdrowowicz, Paweł Wityk, Michał Żyndul, Samanta Makurat, Łukasz Golon
    Academic press:
    The Journal of Physical Chemistry B (rok: 2015, tom: 119, strony: 8227−8238), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.5b03948 - link to the publication
  7. Chemically–enzymatic synthesis of photosensitive DNA
    Authors:
    Kinga Westphal, Magdalena Zdrowowicz, Agnieszka Zylicz-Stachula, Janusz Rak
    Academic press:
    Journal of Photochemistry & Photobiology, B: Biology (rok: 2017, tom: 167, strony: 228–235), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jphotobiol.2017.01.005 - link to the publication
  8. Radiation damage to single stranded oligonucleotide trimers labelled with 5-iodopyrimidines
    Authors:
    Kinga Westphal, Konrad Skotnicki, Krzysztof Bobrowski, Janusz Rak
    Academic press:
    Organic & Biomolecular Chemistry (rok: 2016, tom: 14, strony: 9331-9337), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C6OB01713D - link to the publication

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