Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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3D superresolution microscopy for investigation of chromatin architecture changes of neuronal nuclei in epileptic stress

2012/05/E/ST2/02180

Keywords:

synaptic plasticity chromatin architecture 3D microscopy superresolution image processing

Descriptors:

  • ST6_8: Computer graphics, image processing, computer vision, multimedia, computer games
  • NZ3_6: Cell neurobiology
  • ST2_8: Ultracold atoms and molecules

Panel:

ST2 - Fundamental constituents of matter: particle, nuclear, plasma, atomic, molecular, gas and optical physics

Host institution :

Instytut Biologii Doświadczalnej im. M. Nenckiego PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Tytus Bernas 

Number of co-investigators in the project: 3

Call: SONATA BIS 1 - announced on 2012-03-15

Amount awarded: 1 496 000 PLN

Project start date (Y-m-d): 2013-03-07

Project end date (Y-m-d): 2018-03-06

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Mikroskop wyposażony w: obiektywy, soczewki tubusa, macierze mikrosoczewek, filtry fluorescencyjne, stół antywibracyjny. (180 000 PLN)
  2. Komora laminarna i cieplarka. (50 000 PLN)
  3. Detektor mikroskopowy (kamera) (5 198 PLN)
  4. Lasery. (60 343 PLN)
  5. Stacja robocza PC. (30 000 PLN)

Information in the final report

  • Publication in academic press/journals (14)
  1. Substitution of Metallocenes with [2.2]Paracyclophane to Enable Confocal Microscopy Imaging in Living Cells.
    Authors:
    J. Skiba, C. Schmidt, P. Lipmann, P. Ensllen, A. Wagenknecht, R. Czerwieniec, F. Brandl, I. Ott, T. Bernas, B. Krawczyk, D. Szczukocki, K. Kowalski
    Academic press:
    Eur. J. Inorg. Chemistry (rok: 2016, tom: 2017(2), strony: 297-305), Wydawca: Wiley Periodicals, Inc.
    Status:
    Published
    DOI:
    10.1002/ejic.201600281 - link to the publication
  2. Bafilomycin A1 triggers proliferative potential of senescent cancer cells in vitro and in NOD/SCID mice.
    Authors:
    H. Was, K. Barszcz, J. Czarnecka, A. Kowalczyk, T. Bernas, E. Uzarowska, P. Koza, A. Klejman, K. Piwocka, B. Kaminska, E. Sikora.
    Academic press:
    Oncotarget (rok: 2016, tom: 8(6), strony: 9303-9322), Wydawca: Impact Journals
    Status:
    Published
    DOI:
    10.18632/oncotarget.14066 - link to the publication
  3. Increasing microscopy resolution with photobleaching and intensity cumulant analysis.
    Authors:
    W. Brutkowski, D. Dziob, T. Bernas
    Academic press:
    Micr. Res. Tech. (rok: 2015, tom: 78(11), strony: 958-68), Wydawca: Wiley Periodicals, Inc.
    Status:
    Published
    DOI:
    10.1002/jemt.22559 - link to the publication
  4. Inhibition of stearoyl-CoA desaturase impairs palmitate-derived autophagy at the step of autophagosome-lysosome fusion in pancreatic beta-cells
    Authors:
    J. Janikiewicz, K. Hanzelka, A. Dziewulska, K. Kozinski, P. Dobrzyn, T. Bernas, A. Dobrzyn
    Academic press:
    J. Lipid. Res. (rok: 2015, tom: 56(10), strony: 1901-1911), Wydawca: ASBMB
    Status:
    Published
    DOI:
    10.1194/jlr.M059980 - link to the publication
  5. Pyrene–nucleobase conjugates: synthesis, oligonucleotide binding and confocal bioimaging studies.
    Authors:
    A. Jablonski, Y. Fritz, A. Wagenknecht, R. Czerwieniec, T. Bernas, D. Trzybinski, K. Wozniak, K. Kowalski
    Academic press:
    Beilstein J. Org. Chem. (rok: 2017, tom: 13, strony: 2521–2534), Wydawca: Beilstein
    Status:
    Published
    DOI:
    10.3762/bjoc.13.249 - link to the publication
  6. Some chemotherapeutics-treated colon cancer cells display a specific phenotype being a combination of stem-like and senescent cell features
    Authors:
    H. Was; J. Czarnecka; A. Kowalczyk; K. Barszcz; T. Bernas; K. Piwocka, B. Kaminska
    Academic press:
    Cancer. Biol. Therap. (rok: 2018, tom: 19(1), strony: 63-75), Wydawca: Taylor & Francis
    Status:
    Published
    DOI:
    10.1080/15384047.2017.1385675. - link to the publication
  7. Spatial heterogeneity of dynamics of H1 linker histone.
    Authors:
    T. Bernas, W. Brutkowski, M. Zarebski, J. Dobrucki
    Academic press:
    European Journal of Biophysics (rok: 2014, tom: 43, strony: 287-300), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00249-014-0962-0 - link to the publication
  8. Application of detector precision characteristics for the denoising of biological micrographs in the wavelet domain.
    Authors:
    T. Bernas, R. Starosolski, R. Wójcicki
    Academic press:
    Biomedical Signal Processing and Control (rok: 2015, tom: 19, strony: 45304), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bspc.2015.02.010 - link to the publication
  9. Analysis of spatial correlations between patterns of DNA Damage Response and DNA replication in nuclei of cells subjected to replication stress or oxidative damage.
    Authors:
    T. Bernas, K. Berniak, P. Rybak, M. Zarębski, H. Zhao, Z. Darzynkiewicz, J. W. Dobrucki.
    Academic press:
    Cytometry (rok: 2013, tom: 83 (10), strony: 925 - 932), Wydawca: Willey
    Status:
    Published
    DOI:
    10.1002/cyto.a.22325 - link to the publication
  10. Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis.
    Authors:
    2.K. Berniak, P. Rybak, T. Bernas, M. Zarębski, E. Biela, H. Zhao, Z. Darzynkiewicz, J. W. Dobrucki.
    Academic press:
    Cytometry (rok: 2013, tom: 83 (10), strony: 913 - 924), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22327 - link to the publication
  11. Automatic image alignment and distortion corrections in Light Field Microscopy
    Authors:
    B. Ruszczycki, T. Bernas
    Academic press:
    Microscopy research and Technique (rok: 2018, tom: a/a, strony: ), Wydawca: Willey
    Status:
    Submitted
    DOI:
    brak - link to the publication
  12. Luminescent rhenium(I)–chromone bioconjugate: Synthesis, photophysical properties, and confocal luminescence microscopy investigation.
    Authors:
    K. Kowalski, L. Szczupak, T. Bernas, R. Czerwieniec
    Academic press:
    J. Organometallic. Chemistry (rok: 2015, tom: 782 (15), strony: 124 - 130), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jorganchem.2015.01.017 - link to the publication
  13. Quality of biological images, reconstructed using localization microscopy data.
    Authors:
    B. Ruszczycki, T. Bernas
    Academic press:
    Bioinformatics (rok: 2018, tom: 34(5), strony: 845-852), Wydawca: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/bioinformatics/btx597 - link to the publication
  14. Low level phosphorylation of histone H2AX on serine 139 (γH2AX) is not associated with DNA double-strand breaks.
    Authors:
    P. Rybak, A. Hoang, L. Bujnowicz, T. Bernas, K. Berniak, M. Zarebski, Z. Darzynkiewicz, J. Dobrucki
    Academic press:
    Oncotarget (rok: 2016, tom: 7(31), strony: 49574-49587), Wydawca: Impact Journals
    Status:
    Published
    DOI:
    10.18632/oncotarget.10411 - link to the publication

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