Projects funded by the NCN


Information on the principal investigator and host institution

Information of the project and the call

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Heterologous expression of an alpha-amylase gene in recombinant Yarrowia lipolytica yeast cells.

2012/05/N/NZ1/00002

Keywords:

yarrowia lipolytica alpha-amylase expression vector heterologous expression extracellular transport

Descriptors:

  • NZ1_5: Genetic engineering
  • NZ1_9: Biotechnology
  • NZ1_2: Biochemistry

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Przyrodniczy w Poznaniu, Wydział Nauk o Żywności i Żywieniu

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Ewelina Celińska 

Number of co-investigators in the project: 3

Call: PRELUDIUM 3 - announced on 2012-03-15

Amount awarded: 97 500 PLN

Project start date (Y-m-d): 2013-02-11

Project end date (Y-m-d): 2016-02-10

Project duration:: 36 months (the same as in the proposal)

Project status: Project settled

Information in the final report

  • Publication in academic press/journals (3)
  • Articles in post-conference publications (1)
  1. Cloning, expression, and purification of insect (Sitophilus oryzae) alpha-amylase, able to digest granular starch, in Yarrowia lipolytica host
    Authors:
    Celińska E, Białas W, Borkowska M, Grajek W.
    Academic press:
    Applied Microbiology and Biotechnology (rok: 2015, tom: 99, strony: 2727–2739), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00253-014-6314-2 - link to the publication
  2. Evaluation of heterologous alpha-amylase production in two expression platforms dedicated for Yarrowia lipolytica - commercial Po1g-pYLSC (php4d) and custom-made A18-pYLTEF (pTEF)
    Authors:
    Celińska E, Borkowska M, Białas W.
    Academic press:
    YEAST (rok: 2016, tom: XXX, strony: XXX), Wydawca: WILEY-BLACKWELL
    Status:
    Published
    DOI:
    10.1002/yea.3149 - link to the publication
  3. Evaluation of a recombinant insect-derived amylase performance in simultaneous saccharification and fermentation process with industrial yeasts.
    Authors:
    Celińska E, Borkowska M, Białas W.
    Academic press:
    Applied Microbiology and Biotechnology (rok: 2016, tom: XXX, strony: XXX), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00253-015-7098-8 - link to the publication
  1. "Bug-O-Yeast" - Expression of an insect amylolytic gene in Yarrowia lipolytica
    Authors:
    Celińska E, Borkowska M, Białas W.
    Conference:
    27th International Conference on Yeast Genetics and Molecular Biology (ICYGMB) (rok: 2015, ), Wydawca: WILEY-BLACKWELL
    Data:
    konferencja 06-12/09/2015
    Status:
    Published