Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Unique secretion system of periodontopathogens

2012/04/A/NZ1/00051

Keywords:

P. gingivalis secretion system PerioGate virulence

Descriptors:

  • NZ6_4: Bacteriology
  • NZ1_2: Biochemistry
  • NZ1_1: Molecular biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jan Potempa 

Number of co-investigators in the project: 8

Call: MAESTRO 2 - announced on 2011-12-15

Amount awarded: 2 981 498 PLN

Project start date (Y-m-d): 2012-09-04

Project end date (Y-m-d): 2017-09-03

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Constans System (150 000 PLN)
  2. Komora beztlenowa Whitley A 85 Anaerobic Workstation.
  3. System do analizy ekspresji genów (450 000 PLN)
  4. Termocykler PCR wieloblokowy (35 000 PLN)
  5. Wytrząsarka ze stabilizacją temperatury.
  6. Termocykler 96 dołkowy.
  7. Termocykler PCR gradientowy (35 000 PLN)
  8. Wirówka laboratoryjna z chłodzeniem (52 572 PLN)
  9. Wytwornica lodu (20 000 PLN)
  10. Wytrząsarka hodowlana wraz z kompletem uchwytów (2 szt.) (12 000 PLN)

Information in the final report

  • Publication in academic press/journals (22)
  1. A novel mechanism of latency in matrix metalloproteinases.
    Authors:
    López-Pelegrín M, Ksiazek M, Karim AY, Guevara T, Arolas JL, Potempa J, Gomis-Rüth FX
    Academic press:
    J Biol Chem (rok: 2015, tom: 290, strony: 4728-4740), Wydawca: ASBMB
    Status:
    Published
    DOI:
    10.1074/jbc.M114.605956 - link to the publication
  2. Mirolase, a novel subtilisin-like serine protease from the periodontopathogen Tannerella forsythia
    Authors:
    Ksiazek M, Karim AY, Bryzek D, Enghild JJ, Thøgersen IB, Koziel J, Potempa J.
    Academic press:
    Biol Chem. (rok: 2015, tom: 396, strony: 261-275), Wydawca: degruyter
    Status:
    Published
    DOI:
    10.1515/hsz-2014-0256 - link to the publication
  3. Structure and mechanism of cysteine peptidase Kgp, a major virulence factor of Porphyromonas gingivalis in periodontitis.
    Authors:
    de Diego I, Veillard F, Sztukowska MN, Guevara T, Potempa B, Pomowski A, Huntington JA, Potempa J, Gomis-Rüth FX.
    Academic press:
    J Biol Chem (rok: 2014, tom: 289, strony: 32291-32302), Wydawca: ASMBM
    Status:
    Published
    DOI:
    10.1074/jbc.M114.602052 - link to the publication
  4. Zebrafish as a new model to study effects of periodontal pathogens on cardiovascular diseases.
    Authors:
    Widziolek M, Prajsnar TK, Tazzyman S, Stafford GP, Potempa J, Murdoch C.
    Academic press:
    Scientific Reports (rok: 2016, tom: 6, strony: 36023), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/srep36023 - link to the publication
  5. Genome sequence of Porphyromonas gingivalis strain HG66 (DSM 28984)
    Authors:
    Siddiqui, H., Yoder-Himes, D., Mizgalska, D., Nguyen, K.A., Potempa, J., and Olsen, I.
    Academic press:
    Genome Announcement (GenomeA) (rok: 2014, tom: 2, strony: e00947-14), Wydawca: ASM
    Status:
    Published
    DOI:
    10.1128/genomeA.00947-14 - link to the publication
  6. Inhibition of gingipains by their profragments as the mechanism protecting Porphyromonas gingivalis against premature activation of secreted proteases.
    Authors:
    Veillard F, Sztukowska M, Mizgalska D, Ksiazek M, Houston J, Potempa B, Enghild JJ, Thogersen IB, Gomis-Rüth FX, Nguyen KA, Potempa J.
    Academic press:
    Biochim Biophys Acta (rok: 2013, tom: 1830, strony: 4218-4228), Wydawca: Elsvier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbagen.2013.04.005 - link to the publication
  7. Porphyromonas gingivalis virulence factor gingipain RgpB shows a unique zymogenic mechanism for cysteine peptidases.
    Authors:
    de Diego I, Veillard FT, Guevara T, Potempa B, Sztukowska M, Potempa J, Gomis-Rüth FX.
    Academic press:
    J. Biol. Chem. (rok: 2013, tom: 288, strony: 14287-14296), Wydawca: ASBMB
    Status:
    Published
    DOI:
    10.1074/jbc.M112.444927 - link to the publication
  8. Purification and characterisation of recombinant His-tagged RgpB gingipain from Porphymonas gingivalis.
    Authors:
    Veillard F, Potempa B, Guo Y, Ksiazek M, Sztukowska MN, Houston JA, Koneru L, Nguyen KA, Potempa J
    Academic press:
    Biological Chemistry (rok: 2015, tom: 396, strony: 377-384), Wydawca: De Gruyter
    Status:
    Published
    DOI:
    10.1515/hsz-2014-0304. - link to the publication
  9. Structure and mechanism of a bacterial host-protein citrullinating virulence factor, Porphyromonas gingivalis peptidylarginine deiminase.
    Authors:
    Goulas T, Mizgalska D, Garcia-Ferrer I, Kantyka T, Guevara T, Szmigielski B, Sroka A, Millán C, Usón I, Veillard F, Potempa B, Mydel P, Solà M, Potempa J, Gomis-Rüth FX
    Academic press:
    Scientific Reports (rok: 2015, tom: 5, strony: 11969), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/srep11969 - link to the publication
  10. The S-layer proteins of Tannerella forsythia are secreted via a type IX secretion system that is decoupled from protein O-glycosylation.
    Authors:
    Tomek MB, Neumann L, Nimeth I, Koerdt A, Andesner P, Messner P, Mach L, Potempa JS, Schäffer C.
    Academic press:
    Mol Oral Biol (rok: 2014, tom: 29, strony: 307-320), Wydawca: John Wiley & Sons Ltd.
    Status:
    Published
    DOI:
    10.1111/omi.12062 - link to the publication
  11. The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function.
    Authors:
    Lasica AM, Ksiazek M, Madej M, Potempa J.
    Academic press:
    Front Cell Infect Microbiol. (rok: 2017, tom: 7, strony: 215), Wydawca: www.frointierson.org
    Status:
    Published
    DOI:
    10.3389/fcimb.2017.00215 - link to the publication
  12. The outer-membrane export signal of Porphyromonas gingivalis type IX secretion system (T9SS) is a conserved C-terminal β-sandwich domain.
    Authors:
    de Diego I, Ksiazek M, Mizgalska D, Koneru L, Golik P, Szmigielski B, Nowak M, Nowakowska Z, Potempa B, Houston JA, Enghild JJ, Thøgersen IB, Gao J, Kwan AH, Trewhella J, Dubin G, Gomis-Rüth FX, Nguyen KA, Potempa J.
    Academic press:
    Scientific Reports (rok: 2016, tom: 6, strony: 23123), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/srep23123 - link to the publication
  13. Sequence-independent processing site of the C-terminal domain (CTD) influences maturation of the RgpB protease from Porphyromonas gingivalis
    Authors:
    Zhou XY, Gao JL, Hunter N, Potempa J, Nguyen KA.
    Academic press:
    Mol Microbiol. 2013 Sep;89(5):. doi: (rok: 2013, tom: 89, strony: 903-917), Wydawca: John Wiley & Sons Ltd
    Status:
    Published
    DOI:
    10.1111/mmi.12319 - link to the publication
  14. Structural and functional probing of PorZ, an essential bacterial surface component of the type-IX secretion system of human oral-microbiomic Porphyromonas gingivalis.
    Authors:
    Lasica AM, Goulas T, Mizgalska D, Zhou X, de Diego I, Ksiazek M, Madej M, Guo Y, Guevara T, Nowak M, Potempa B, Goel A, Sztukowska M, Prabhakar AT, Bzowska M, Widziolek M, Thøgersen IB, Enghild JJ, Simonian M, Kulczyk AW, Nguyen KA, Potempa J, Gomis-Rüth FX.
    Academic press:
    Scientific Reports (rok: 2016, tom: 6, strony: 37708), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/srep37708 - link to the publication
  15. Structure of RagB, a major immunodominant outer-membrane surface receptor antigen of Porphyromonas gingivalis.
    Authors:
    Goulas T, Garcia-Ferrer I, Hutcherson JA, Potempa BA, Potempa J, Scott DA, Xavier Gomis-Rüth F.
    Academic press:
    Molecular Oral Microbiology (rok: 2016, tom: 31, strony: 472-485), Wydawca: John Wiley & Sons
    Status:
    Published
    DOI:
    10.1111/omi.12140 - link to the publication
  16. Structure of the catalytic domain of the Tannerella forsythia matrix metallopeptidase karilysin in complex with a tetrapeptidic inhibitor.
    Authors:
    Guevara T, Ksiazek M, Skottrup PD, Cerdà-Costa N, Trillo-Muyo S, de Diego I, Riise E, Potempa J, Gomis-Rüth FX
    Academic press:
    Acta Crystallogr Sect F Struct Biol Cryst Commun (rok: 2013, tom: 69, strony: 472-476), Wydawca: International Union of Crystallography
    Status:
    Published
    DOI:
    10.1107/S1744309113007392 - link to the publication
  17. Role of OmpA2 surface regions of Porphyromonas gingivalis in host-pathogen interactions with oral epithelial cells.
    Authors:
    Naylor KL, Widziolek M, Hunt S, Conolly M, Hicks M, Stafford P, Potempa J, Murdoch C, Douglas CW, Stafford GP.
    Academic press:
    Microbiologyopen (rok: 2017, tom: 6, strony: e00401), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/mbo3.401 - link to the publication
  18. A Metalloproteinase Mirolysin of Tannerella forsythia Inhibits All Pathways of the Complement System.
    Authors:
    Jusko M, Potempa J, Mizgalska D, Bielecka E, Ksiazek M, Riesbeck K, Garred P, Eick S, Blom AM
    Academic press:
    Journal of Immunology (rok: 2015, tom: 195, strony: 2231-2240), Wydawca: The American Association of Immunologists, Inc.
    Status:
    Published
    DOI:
    10.4049/jimmunol.1402892 - link to the publication
  19. A structure-derived snap-trap mechanism of a multispecific serpin from the dysbiotic human oral microbiome.
    Authors:
    Goulas T, Ksiazek M, Garcia-Ferrer I, Sochaj-Gregorczyk AM, Waligorska I, Wasylewski M, Potempa J, Gomis-Rüth FX.
    Academic press:
    J. Biol. Chem. (rok: 2017, tom: 292, strony: 10883-10898), Wydawca: ASBMB
    Status:
    Published
    DOI:
    10.1074/jbc.M117.786533 - link to the publication
  20. Calcium Regulates the Activity and Structural Stability of Tpr, a Bacterial Calpain-like Peptidase
    Authors:
    Staniec D, Ksiazek M, Thøgersen IB, Enghild JJ, Sroka A, Bryzek D, Bogyo M, Abrahamson M, Potempa J
    Academic press:
    Journal of Biological Chemistry (rok: 2015, tom: 290, strony: 27248-27260), Wydawca: The American Society for Biochemistry and Molecular Biology, Inc.
    Status:
    Published
    DOI:
    10.1074/jbc.M115.648782 - link to the publication
  21. KLIKK proteases of Tannerella forsythia: putative virulence factors with a unique domain structure.
    Authors:
    Ksiazek M, Mizgalska D, Eick S, Thøgersen IB, Enghild JJ, Potempa J
    Academic press:
    Frontiers in Microbiology (rok: 2015, tom: 6, strony: 312), Wydawca: Open Access Frontiers
    Status:
    Published
    DOI:
    10.3389/fmicb.2015.00312 - link to the publication
  22. The essential Porphyromonas gingivalis type IX secretion system component PorZ delivers anionic-lipopolysaccharide to the PorU sortase for transpeptidase processing of cargos
    Authors:
    Mariusz Madej, Zuzanna Nowakowska, Miroslaw Ksiazek, Anna M. Lasica, Danuta Mizgalska, Magdalena Nowak, Anna Jacula, Carsten Scaveniusd, Jan J. Enghild, Joseph Aduse-Opoku, Michael A. Curtis, F. Xavier Gomis-Rüth, and Jan Potempa
    Academic press:
    mBio , Wydawca: ASM
    Status:
    Submitted
    DOI:
    brak - link to the publication

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