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Information on the principal investigator and host institution

Information of the project and the call

Keywords

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tRNA transcription: novel layers of regulation

2012/04/A/NZ1/00052

Keywords:

transcription RNA polymerase tRNA yeast

Descriptors:

  • NZ1_1: Molecular biology
  • NZ2_1: Molecular genetics

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Biochemii i Biofizyki PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Magdalena Rakowska-Boguta 

Number of co-investigators in the project: 4

Call: MAESTRO 2 - announced on 2011-12-15

Amount awarded: 2 931 000 PLN

Project start date (Y-m-d): 2012-09-17

Project end date (Y-m-d): 2017-11-16

Project duration:: 62 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. inkubator mikrobiologiczny z wytrząsaniem i chłodzeniem Innova 42 R wraz z platformą i kompletem uchwytów (53 000 PLN)
  2. wirówka laboratoryjna z chłodzeniem, rotor na probówki 1,5/2 ml min.14000 rpm, rotor na probówki 15/50 ml min. 4000 rpm (25 000 PLN)
  3. wirówka stołowa Eppendorf MiniSpin z rotorem (4 500 PLN)
  4. cieplarka mikrobiologiczna z chłodzeniem Memmert (11 000 PLN)
  5. thermomixer Comfort (8 500 PLN)
  6. zestaw do elektroforezy wertykalnej: aparat Mini-PROTEAN Tetra Cell system z modułem do transferu białek Mini Trans-Blot Module i zasilaczem PowerPac Basic Power Supply (9 800 PLN)
  7. termocykler z gradientem temperatur BioRad C1000 z blokiem grzejnym Dual 48/48 do prowadzenia reakcji PCR (43 000 PLN)
  8. piec do hybrydyzacji membran z RNA z sondami oligonukleotydowymi znakowanymi radioizotopowo (15 000 PLN)

Information in the final report

  • Publication in academic press/journals (9)
  • Articles in post-conference publications (10)
  1. Function of TFIIIC, RNA polymerase III initiation factor, in acivation and repression of tRNA gene transcription
    Authors:
    Cieśla M, Skowronek E & Boguta M
    Academic press:
    Nucleic Acids Research (rok: 2018, tom: 46, strony: 9444-9455), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gky656. - link to the publication
  2. Rbs1, a new protein implicated in RNA polymerase III biogenesis in yeast Saccharomyces cerevisiae
    Authors:
    Cieśla M, Ewa Makała E, Płonkaa M, Bazan R, Gewartowski K, Dziembowski A & Boguta M
    Academic press:
    Molecular and Cellular Biology (rok: 2015, tom: 35, strony: 1169-1181), Wydawca: American Society for Microbiology
    Status:
    Published
    DOI:
    10.1128/MCB.01230-14 - link to the publication
  3. Regulation of tRNA synthesis by the general transcription factors of RNA polymerase III - TFIIIB and TFIIIC, and by the MAF1 protein
    Authors:
    Graczyk D, Ciesla M & Boguta M
    Academic press:
    BBA-Gene Regulatory Mechanisms (rok: 2018, tom: 1861, strony: 320-329), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagrm.2018.01.011 - link to the publication
  4. Control of S.cerevisiae pre-tRNA processing by environmental conditions
    Authors:
    Foretek D, Wu J, Hopper AK & Boguta M
    Academic press:
    RNA Journal (rok: 2016, tom: 22, strony: 449-359), Wydawca: RNA Society
    Status:
    Published
    DOI:
    10.1261/rna.054973.115. - link to the publication
  5. Why Are tRNAs Overproduced in the Absence of Maf1, a Negative Regulator of RNAP III, Not Fully Functional
    Authors:
    Boguta M
    Academic press:
    PLOS Genetics (rok: 2015, tom: 11, strony: 11(12):e1005743), Wydawca: The Public Library of Science (PLOS)
    Status:
    Published
    DOI:
    10.1371/journal.pgen.1005743 - link to the publication
  6. Global analysis of transcriptionally engaged yeast RNA polymerase III reveals extended tRNA transcripts
    Authors:
    Turowski T, Leśniewska E, Delan-Forino C, Boguta M & Tollervey D
    Academic press:
    Genome Research (rok: 2016, tom: 7, strony: 933-944), Wydawca: Cold Spring Harbor Laboratory Press
    Status:
    Published
    DOI:
    10.1101/gr.205492.116. - link to the publication
  7. Repression of yeast RNA polymerase III by stress leads to ubiquitylation and proteasomal degradation of its largest subunit, C160
    Authors:
    Leśniewska E, Cieśla M, Boguta M
    Academic press:
    BBA-Gene Regulatory Mechanisms (rok: 2019, tom: 1862, strony: 25-34), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.007 - link to the publication
  8. Novel layers of RNA polymerase III control affecting tRNA gene transcription in eukaryotes
    Authors:
    Leśniewska E & Boguta M
    Academic press:
    Open Biology (rok: 2017, tom: pii: 170001, strony: .), Wydawca: The Royal Society
    Status:
    Published
    DOI:
    10.1098/rsob.170001 - link to the publication
  9. An interplay between transcription, processing, and degradation determines tRNA levels in yeast
    Authors:
    Wichtowska D, Turowski T & Boguta M
    Academic press:
    Wiley Interdisciplinary Reviews: RNA (rok: 2013, tom: 4(6), strony: 709-722), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/wrna.1190 - link to the publication
  1. Function of TFIIIC, RNA polymerase III initiation factor, in activation and repression of tRNA gene transcription in yeast
    Authors:
    Cieśla M, Skowronek E & Boguta M
    Conference:
    OddPols 2018 International Conference on Transcription by RNA polymerases I, II, IV and V (rok: 2018, ), Wydawca: CBI (Toulouse Centre for Biologie Integrative)
    Data:
    konferencja 26-29 czerwca
    Status:
    Published
    DOI:
    link to the publication
  2. tRNA transcription: novel layers of regulation
    Authors:
    Makała E, Turowski T, Tollervey D & Boguta M
    Conference:
    Gene Transcription in Yeast: From Chromatin to RNA and back (rok: 2016, ), Wydawca: EMBO Excellence in Life Sciences
    Data:
    konferencja 11-16 czerwca
    Status:
    Published
  3. Coupling of RNA polymerase III assembly and cell-cycle control in Saccharomyces cerevisiae
    Authors:
    Cieśla M, Płonka M, Wawrzycka D, Wysocki R & Boguta M
    Conference:
    27th International Conference on Yeast Genetics and Molecular Biology (ICYGMB), (rok: 2015, ), Wydawca: Willey Blackwell
    Data:
    konferencja 6-12 września
    Status:
    Published
  4. tRNA transcription: novel layers of regulation
    Authors:
    Makała E, Turowski T, Tollervey D & Boguta M
    Conference:
    Bio2016 (rok: 2016, ), Wydawca: Acta Biochimica Polonica
    Data:
    konferencja 13-16 września
    Status:
    Published
  5. A subunit of transcription factor TFIIIC is controlled by PKA signaling pathway and thereby mediates regulation of RNA polymerase III transcription by carbon source
    Authors:
    Ciesla M, Skowronek E, Kepka M & Boguta M
    Conference:
    42-th FEBS Congeress: From Molecules to Cells and Back (rok: 2017, ), Wydawca: FEBS
    Data:
    konferencja 10-14 września
    Status:
    Published
  6. Global analysis of transcriptionally engaged yeast Pol III reveals unequal distribution of polymerase on transcription units and extended tRNA transcripts
    Authors:
    Makała E,, Turowski T, Tollervey D & Boguta M
    Conference:
    OddPols 2016 International Conference on Transcription by RNA polymerases I, II, IV and V (rok: 2016, ), Wydawca: The University of Michigan
    Data:
    konferencja 24-27 czerwca
    Status:
    Published
  7. Proteasome-mediated degradation of Rpc160, the largest subunit of RNA polymerase III, in the response to stress
    Authors:
    Makała E, Rudzińska I & Boguta M
    Conference:
    7th Proteasomes & Autophagy Workshop (rok: 2016, ), Wydawca: Manifestation & Organisation, Ceyrat, France
    Data:
    konferencja 6-8 kwietnia
    Status:
    Published
  8. Exploring the effects of the immunosuppressant drug, mycophenolic acid, on transcription by RNA polymerase III
    Authors:
    Leśniewska E, Jurkiewicz A, Cieśla M, Graczyk D & and Boguta M
    Conference:
    OddPols 2018 International Conference on Transcription by RNA polymerases I, II, IV and V (rok: 2018, ), Wydawca: CBI (Toulouse Centre for Biologie Integrative)
    Data:
    konferencja 26-29 czerwca
    Status:
    Published
    DOI:
    link to the publication
  9. RAS/PKA signaling pathway is involved in regulation of RNA polymerase IIItranscription mediated by TFIIIC factor
    Authors:
    Kępka M, Cieśla M & Boguta M
    Conference:
    27th International Conference on Yeast Genetics and Molecular Biology (ICYGMB) (rok: 2015, ), Wydawca: Willey Blackwell
    Data:
    konferencja 6-12 września
    Status:
    Published
  10. Stress conditions result in down-regulation of RNA polymerase III activity and degradation of its largest subunit by ubiquitine/proteasome system
    Authors:
    Leśniewska E & Boguta M
    Conference:
    42-th FEBS Congress: From Molecules to Cells and Back (rok: 2017, ), Wydawca: FEBS
    Data:
    konferencja 10-14 września
    Status:
    Published

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