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tRNA transcription: novel layers of regulation

2012/04/A/NZ1/00052

Keywords

Descriptors

  • NZ1_1: Biologia molekularna
  • NZ2_1: Genetyka molekularna

Panel

NZ1 – Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution

Instytut Biochemii i Biofizyki PAN

Voivodeship: mazowieckie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Magdalena Rakowska-Boguta

Number of co-investigators in the project: 4

Call

MAESTRO 2 – announced on 2011-12-15

Amount awarded
2 931 000 PLN
Project start date
2012-09-17
Project end date
2017-11-16
Project duration
62 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. cieplarka mikrobiologiczna z chłodzeniem Memmert. Cost: 11 000 PLN
  2. inkubator mikrobiologiczny z wytrząsaniem i chłodzeniem Innova 42 R wraz z platformą i kompletem uchwytów. Cost: 53 000 PLN
  3. piec do hybrydyzacji membran z RNA z sondami oligonukleotydowymi znakowanymi radioizotopowo. Cost: 15 000 PLN
  4. termocykler z gradientem temperatur BioRad C1000 z blokiem grzejnym Dual 48/48 do prowadzenia reakcji PCR. Cost: 43 000 PLN
  5. thermomixer Comfort. Cost: 8 500 PLN
  6. wirówka laboratoryjna z chłodzeniem, rotor na probówki 1,5/2 ml min.14000 rpm, rotor na probówki 15/50 ml min. 4000 rpm. Cost: 25 000 PLN
  7. wirówka stołowa Eppendorf MiniSpin z rotorem. Cost: 4 500 PLN
  8. zestaw do elektroforezy wertykalnej: aparat Mini-PROTEAN Tetra Cell system z modułem do transferu białek Mini Trans-Blot Module i zasilaczem PowerPac Basic Power Supply. Cost: 9 800 PLN

Information in the final report

Publication in academic press/journals (9)

  1. Rbs1, a new protein implicated in RNA polymerase III biogenesis in yeast Saccharomyces cerevisiae

    Authors:
    Cieśla M, Ewa Makała E, Płonkaa M, Bazan R, Gewartowski K, Dziembowski A & Boguta M
    Academic press:
    Molecular and Cellular Biology (year: 2015, volume: 35, pages: 1169-1181), Publisher: American Society for Microbiology
    Status:
    Published
    DOI:
    10.1128/MCB.01230-14 – link to the publication

  2. Why Are tRNAs Overproduced in the Absence of Maf1, a Negative Regulator of RNAP III, Not Fully Functional

    Authors:
    Boguta M
    Academic press:
    PLOS Genetics (year: 2015, volume: 11, pages: 11(12):e1005743), Publisher: The Public Library of Science (PLOS)
    Status:
    Published
    DOI:
    10.1371/journal.pgen.1005743 – link to the publication

  3. Control of S.cerevisiae pre-tRNA processing by environmental conditions

    Authors:
    Foretek D, Wu J, Hopper AK & Boguta M
    Academic press:
    RNA Journal (year: 2016, volume: 22, pages: 449-359), Publisher: RNA Society
    Status:
    Published
    DOI:
    10.1261/rna.054973.115. – link to the publication

  4. Global analysis of transcriptionally engaged yeast RNA polymerase III reveals extended tRNA transcripts

    Authors:
    Turowski T, Leśniewska E, Delan-Forino C, Boguta M & Tollervey D
    Academic press:
    Genome Research (year: 2016, volume: 7, pages: 933-944), Publisher: Cold Spring Harbor Laboratory Press
    Status:
    Published
    DOI:
    10.1101/gr.205492.116. – link to the publication

  5. Function of TFIIIC, RNA polymerase III initiation factor, in acivation and repression of tRNA gene transcription

    Authors:
    Cieśla M, Skowronek E & Boguta M
    Academic press:
    Nucleic Acids Research (year: 2018, volume: 46, pages: 9444-9455), Publisher: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gky656. – link to the publication

  6. Regulation of tRNA synthesis by the general transcription factors of RNA polymerase III - TFIIIB and TFIIIC, and by the MAF1 protein

    Authors:
    Graczyk D, Ciesla M & Boguta M
    Academic press:
    BBA-Gene Regulatory Mechanisms (year: 2018, volume: 1861, pages: 320-329), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagrm.2018.01.011 – link to the publication

  7. Repression of yeast RNA polymerase III by stress leads to ubiquitylation and proteasomal degradation of its largest subunit, C160

    Authors:
    Leśniewska E, Cieśla M, Boguta M
    Academic press:
    BBA-Gene Regulatory Mechanisms (year: 2019, volume: 1862, pages: 25-34), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagrm.2018.10.007 – link to the publication

  8. Novel layers of RNA polymerase III control affecting tRNA gene transcription in eukaryotes

    Authors:
    Leśniewska E & Boguta M
    Academic press:
    Open Biology (year: 2017, volume: pii: 170001, pages: .), Publisher: The Royal Society
    Status:
    Published
    DOI:
    10.1098/rsob.170001 – link to the publication

  9. An interplay between transcription, processing, and degradation determines tRNA levels in yeast

    Authors:
    Wichtowska D, Turowski T & Boguta M
    Academic press:
    Wiley Interdisciplinary Reviews: RNA (year: 2013, volume: 4(6), pages: 709-722), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/wrna.1190 – link to the publication

Articles in post-conference publications (10)

  1. tRNA transcription: novel layers of regulation

    Authors:
    Makała E, Turowski T, Tollervey D & Boguta M
    Conference:
    Bio2016 (year: 2016, pages: 25), Publisher: Acta Biochimica Polonica
    Date:
    conference 13-16 września
    Status:
    Published

  2. RAS/PKA signaling pathway is involved in regulation of RNA polymerase IIItranscription mediated by TFIIIC factor

    Authors:
    Kępka M, Cieśla M & Boguta M
    Conference:
    27th International Conference on Yeast Genetics and Molecular Biology (ICYGMB) (year: 2015, pages: S105-106), Publisher: Willey Blackwell
    Date:
    conference 6-12 września
    Status:
    Published

  3. Proteasome-mediated degradation of Rpc160, the largest subunit of RNA polymerase III, in the response to stress

    Authors:
    Makała E, Rudzińska I & Boguta M
    Conference:
    7th Proteasomes & Autophagy Workshop (year: 2016, pages: 190), Publisher: Manifestation & Organisation, Ceyrat, France
    Date:
    conference 6-8 kwietnia
    Status:
    Published

  4. Coupling of RNA polymerase III assembly and cell-cycle control in Saccharomyces cerevisiae

    Authors:
    Cieśla M, Płonka M, Wawrzycka D, Wysocki R & Boguta M
    Conference:
    27th International Conference on Yeast Genetics and Molecular Biology (ICYGMB), (year: 2015, pages: S95), Publisher: Willey Blackwell
    Date:
    conference 6-12 września
    Status:
    Published

  5. Function of TFIIIC, RNA polymerase III initiation factor, in activation and repression of tRNA gene transcription in yeast

    Authors:
    Cieśla M, Skowronek E & Boguta M
    Conference:
    OddPols 2018 International Conference on Transcription by RNA polymerases I, II, IV and V (year: 2018, pages: 43), Publisher: CBI (Toulouse Centre for Biologie Integrative)
    Date:
    conference 26-29 czerwca
    Status:
    Published

  6. Stress conditions result in down-regulation of RNA polymerase III activity and degradation of its largest subunit by ubiquitine/proteasome system

    Authors:
    Leśniewska E & Boguta M
    Conference:
    42-th FEBS Congress: From Molecules to Cells and Back (year: 2017, pages: 16234), Publisher: FEBS
    Date:
    conference 10-14 września
    Status:
    Published

  7. A subunit of transcription factor TFIIIC is controlled by PKA signaling pathway and thereby mediates regulation of RNA polymerase III transcription by carbon source

    Authors:
    Ciesla M, Skowronek E, Kepka M & Boguta M
    Conference:
    42-th FEBS Congeress: From Molecules to Cells and Back (year: 2017, pages: 24305), Publisher: FEBS
    Date:
    conference 10-14 września
    Status:
    Published

  8. Exploring the effects of the immunosuppressant drug, mycophenolic acid, on transcription by RNA polymerase III

    Authors:
    Leśniewska E, Jurkiewicz A, Cieśla M, Graczyk D & and Boguta M
    Conference:
    OddPols 2018 International Conference on Transcription by RNA polymerases I, II, IV and V (year: 2018, pages: 57), Publisher: CBI (Toulouse Centre for Biologie Integrative)
    Date:
    conference 26-29 czerwca
    Status:
    Published

  9. tRNA transcription: novel layers of regulation

    Authors:
    Makała E, Turowski T, Tollervey D & Boguta M
    Conference:
    Gene Transcription in Yeast: From Chromatin to RNA and back (year: 2016, pages: 52), Publisher: EMBO Excellence in Life Sciences
    Date:
    conference 11-16 czerwca
    Status:
    Published

  10. Global analysis of transcriptionally engaged yeast Pol III reveals unequal distribution of polymerase on transcription units and extended tRNA transcripts

    Authors:
    Makała E,, Turowski T, Tollervey D & Boguta M
    Conference:
    OddPols 2016 International Conference on Transcription by RNA polymerases I, II, IV and V (year: 2016, pages: 23), Publisher: The University of Michigan
    Date:
    conference 24-27 czerwca
    Status:
    Published

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