Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Plant nuclear genome - its structure, dynamics and evolution from the cytomolecular perspective

2012/04/A/NZ3/00572

Keywords:

Brachypodium B. distachyon chromosome painting chromosome territories epigenetics FISH meiosis mutagenesis nuclear genome evolution plant molecular cytogenetics

Descriptors:

  • NZ2_5: Cell genetics
  • NZ3_1: Cell biology
  • NZ1_1: Molecular biology

Panel:

NZ3 - Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution :

Uniwersytet Śląski w Katowicach, Wydział Biologii i Ochrony Środowiska

woj. śląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Robert Hasterok 

Number of co-investigators in the project: 13

Call: MAESTRO 2 - announced on 2011-12-15

Amount awarded: 2 691 135 PLN

Project start date (Y-m-d): 2012-08-21

Project end date (Y-m-d): 2018-05-20

Project duration:: 69 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Homogenizator ultradźwiękowy przystosowany do próbek o małej objętości w zamkniętych probówkach (85 000 PLN)
  2. Laptop Asus G750JH-T4100H.
  3. Tablet Aspire P3-171.
  4. Termocykler do PCR z trzema niezależnymi blokami.
  5. Wirówka z rotorem na probówki 5 ml.
  6. Mikroskop epifluorescencyjny szerokiego pola wyposażony w ośmiopozycyjny uchwyt filtrów wraz z zestawami wąskopasmowych filtrów fluorescencyjnych oraz kamerą cyfrową wysokiej czułości i oprogramowaniem do akwizycji i cyfrowej obróbki obrazu (495 000 PLN)
  7. Termocykler do PCR z blokiem na 96 probówek 0,2 ml / płytkę PCR z 96 studzienkami i gradientem temperatury (35 000 PLN)
  8. Specjalistyczne oprogramowanie do rekonstrukcji obrazu mikroskopowego 3-D i 4-D (160 000 PLN)

Information in the final report

  • Publication in academic press/journals (19)
  • Book publications / chapters in book publications (2)
  1. Comparatively barcoded chromosomes of Brachypodium perennials tell the story of their karyotype structure and evolution
    Authors:
    Joanna Lusinska, Alexander Betekhtin, Diana Lopez-Alvarez, Pilar Catalan, Glyn Jenkins, Elzbieta Wolny, Robert Hasterok
    Academic press:
    International Journal of Molecular Sciences (rok: 2019, tom: 20, strony: 5557 (1-19)), Wydawca: Multidisciplinary Digital Publishing Institute
    Status:
    Published
    DOI:
    10.3390/ijms20225557 - link to the publication
  2. Detecting Brachypodium distachyon chromosomes Bd4 and Bd5 in MH- and X-ray-induced micronuclei using mcFISH
    Authors:
    Arita Kus, Joanna Szymanowska-Pułka, Jolanta Kwasniewska, Robert Hasterok
    Academic press:
    International Journal of Molecular Sciences (rok: 2019, tom: 20, strony: 2848 (1-13)), Wydawca: Multidisciplinary Digital Publishing Institute
    Status:
    Published
    DOI:
    10.3390/ijms20112848 - link to the publication
  3. Epigenetics of the preferential silencing of Brachypodium stacei-originated 35S rDNA loci in the allotetraploid grass Brachypodium hybridum
    Authors:
    Natalia Borowska-Zuchowska, Robert Hasterok
    Academic press:
    Scientific Reports (rok: 2017, tom: 7, strony: 5260 (1-12)), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-017-05413-x - link to the publication
  4. Gradual polyploid genome evolution revealed by a pan-genomic analysis of Brachypodium hybridum and its diploid progenitors
    Authors:
    Sean P. Gordon, Bruno Contreras-Moreira, Joshua J. Levy, Armin Djamei, Angelika Czedik-Eysenberg, Virginia S. Tartaglio, Adam Session, Joel Martin, Amy Cartwright, Andrew Katz, Vasanth R. Singan, Eugene Goltsman, Kerrie Barry, Vinh Ha Dinh-Thi, Boulos Chalhoub, Antonio Diaz-Perez, Ruben Sancho, Joanna Lusinska, Elzbieta Wolny, Candida Nibau, John H. Doonan, Luis Mur, Chris Plott, Jerry Jenkins, Samuel P. Hazen, Scott J. Lee, Shenquaing Shu, David Goodstein, Daniel Rokhsar, Jeremy Schmutz, Robert Hasterok, Pilar Catalan, John P. Vogel
    Academic press:
    Nature Communications (rok: 2020, ), Wydawca: Nature Publishing Group
    Status:
    Accepted for publication
    DOI:
    Brak - link to the publication
  5. Cytomolecular analysis of ribosomal DNA evolution in a natural allotetraploid Brachypodium hybridum and its putative ancestors — dissecting complex repetitive structure of intergenic spacers
    Authors:
    Natalia Borowska-Zuchowska, Miroslaw Kwasniewski, Robert Hasterok
    Academic press:
    Frontiers in Plant Science (rok: 2016, tom: 7, strony: 1499 (1-15)), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fpls.2016.01499 - link to the publication
  6. Ribosomal DNA loci derived from Brachypodium stacei are switched off for major parts of the life cycle of Brachypodium hybridum
    Authors:
    Natalia Borowska-Zuchowska, Ewa Robaszkiewicz, Elzbieta Wolny, Alexander Betekhtin, Robert Hasterok
    Academic press:
    Journal of Experimental Botany (rok: 2019, tom: 70, strony: 805–815), Wydawca: Oxford University Press on behalf of the Society for Experimental Biology
    Status:
    Published
    DOI:
    10.1093/jxb/ery425 - link to the publication
  7. The arrangement of Brachypodium distachyon chromosomes in interphase nuclei
    Authors:
    Ewa Robaszkiewicz, Dominika Idziak-Helmcke, Magdalena A. Tkacz, Kornel Chrominski, Robert Hasterok
    Academic press:
    Journal of Experimental Botany (rok: 2016, tom: 67, strony: 5571–5583), Wydawca: Oxford Journals
    Status:
    Published
    DOI:
    10.1093/jxb/erw325 - link to the publication
  8. In situ analysis of epigenetic modifications in the chromatin of Brachypodium distachyon embryos
    Authors:
    Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Daria Kroczek, Robert Hasterok
    Academic press:
    Plant Signaling & Behavior (rok: 2015, tom: 10, strony: e1011948 (1-2)), Wydawca: Taylor & Francis Group
    Status:
    Published
    DOI:
    10.1080/15592324.2015.1011948 - link to the publication
  9. Insight into the karyotype evolution of Brachypodium species using comparative chromosome barcoding
    Authors:
    Dominika Idziak, Iwona Hazuka, Beata Poliwczak, Anna Wiszynska, Elzbieta Wolny, Robert Hasterok
    Academic press:
    PLOS ONE (rok: 2014, tom: 9, strony: e93503 (1-10)), Wydawca: Public Library od Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0093503 - link to the publication
  10. Karyotyping of Brachypodium pinnatum (2n = 18) chromosomes using cross-species BAC–FISH
    Authors:
    Elzbieta Wolny, Wojciech Fidyk, Robert Hasterok
    Academic press:
    Genome (rok: 2013, tom: 56, strony: 239-243), Wydawca: NRC Research Press Journal
    Status:
    Published
    DOI:
    10.1139/gen-2013-001 - link to the publication
  11. Reconstructing the evolution of Brachypodium genomes using comparative chromosome painting
    Authors:
    Alexander Betekhtin, Glyn Jenkins, Robert Hasterok
    Academic press:
    PLOS ONE (rok: 2014, tom: 9, strony: e115108 (1-26)), Wydawca: Public Library od Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0115108 - link to the publication
  12. Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination
    Authors:
    Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Robert Hasterok
    Academic press:
    PLOS ONE (rok: 2014, tom: 9, strony: e101246 (1-10)), Wydawca: Public Library of Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0101246 - link to the publication
  13. Update on genomics and basic biology of Brachypodium
    Authors:
    International Brachypodium Initiative (IBI): Pilar Catalan, Boulos Chalhoub, Vincent Chochois, David F. Garvin, Robert Hasterok, Antonio J. Manzaneda, Luis A.J. Mur, Nicola Pecchioni, Søren K. Rasmussen, John P. Vogel, Aline Voxeur
    Academic press:
    Trends in Plant Science (rok: 2014, tom: 19, strony: 414-418), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.tplants.2014.05.002 - link to the publication
  14. Chromosome identification and reconstruction of evolutionary rearrangements in Brachypodium distachyon, B. stacei and B. hybridum
    Authors:
    Joanna Lusinska, Joanna Majka, Alexander Betekhtin, Karolina Susek, Elzbieta Wolny, Robert Hasterok
    Academic press:
    Annals of Botany (rok: 2018, tom: 122, strony: 445–459), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/aob/mcy086 - link to the publication
  15. Chromosome territory modeller and viewer
    Authors:
    Magdalena A. Tkacz, Kornel Chrominski, Dominika Idziak-Helmcke, Ewa Robaszkiewicz, Robert Hasterok
    Academic press:
    PLOS ONE (rok: 2016, tom: 11, strony: e0160303 (1-20)), Wydawca: Public Library od Science
    Status:
    Published
    DOI:
    10.1371/journal. - link to the publication
  16. Dissecting the chromosomal composition of mutagen-induced micronuclei in Brachypodium distachyon using multicolour FISH
    Authors:
    Arita Kus, Jolanta Kwasniewska, Joanna Szymanowska-Pulka, Robert Hasterok
    Academic press:
    Annals of Botany (rok: 2018, tom: 122, strony: 1161–1171), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/aob/mcy115 - link to the publication
  17. Spatial distribution of centromeres and telomeres at interphase varies among Brachypodium species
    Authors:
    Dominika Idziak, Ewa Robaszkiewicz, Robert Hasterok
    Academic press:
    Journal of Experimental Botany (rok: 2015, tom: 66, strony: 6623-6634), Wydawca: Oxford Journals
    Status:
    Published
    DOI:
    0.1093/jxb/erv369 - link to the publication
  18. Brachypodium distachyon - a useful model in the qualification of mutagen-induced micronuclei using multicolor FISH
    Authors:
    Arita Kus, Jolanta Kwasniewska, Robert Hasterok
    Academic press:
    PLOS ONE (rok: 2017, tom: 12, strony: e0170618-1-e0170618-13), Wydawca: Public Library od Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0170618 - link to the publication
  19. Histone H3 and H4 acetylation patterns are more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination
    Authors:
    Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Daria Kroczek, Robert Hasterok
    Academic press:
    Protoplasma (rok: 2017, tom: 254, strony: 2045–2052), Wydawca: Springer Wien
    Status:
    Published
    DOI:
    10.1007/s00709-017-1088-x - link to the publication
  1. Methods for cytogenetic chromosome barcoding and chromosome iainting in Brachypodium distachyon and its relative species
    Authors:
    Dominika Idziak-Helmcke, Alexander Betekhtin
    Book:
    Brachypodium Genomics (Methods and Protocols: Methods in Molecular Biology) (rok: 2018, tom: 1667, strony: 45310), Wydawca: Springer Science+Business Media LLC
    Status:
    Published
  2. Molecular cytogenetics in the genus Brachypodium
    Authors:
    Robert Hasterok, Alexander Betekhtin, Natalia Borowska-Zuchowska, Agnieszka Braszewska-Zalewska, Dominika Idziak-Helmcke, Ewa Robaszkiewicz, Elzbieta Wolny
    Book:
    Genetics and Genomics of Brachypodium (Plant Genetics and Genomics: Crops Models) (rok: 2015, tom: 18, strony: 39-54), Wydawca: Springer International Publishing Switzerland
    Status:
    Published

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