Plant nuclear genome - its structure, dynamics and evolution from the cytomolecular perspective
2012/04/A/NZ3/00572
Keywords
Descriptors
- NZ2_5: Cytogenetyka
- NZ3_1: Biologia komórki
- NZ1_1: Biologia molekularna
Panel
NZ3 – Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology
Host institution
UNIWERSYTET ŚLĄSKI, Wydział Biologii i Ochrony Środowiska
Voivodeship: śląskie
Principal investigator (from the host institution)
Number of co-investigators in the project: 13
Call
MAESTRO 2 – announced on 2011-12-15
- Amount awarded
- 2 691 135 PLN
- Project start date
- 2012-08-21
- Project end date
- 2018-05-20
- Project duration
- 69 months (the same as in the proposal)
- Project status
- Project settled
Equipment purchased [PL]
- Homogenizator ultradźwiękowy przystosowany do próbek o małej objętości w zamkniętych probówkach. Cost: 85 000 PLN
- Laptop Asus G750JH-T4100H.
- Mikroskop epifluorescencyjny szerokiego pola wyposażony w ośmiopozycyjny uchwyt filtrów wraz z zestawami wąskopasmowych filtrów fluorescencyjnych oraz kamerą cyfrową wysokiej czułości i oprogramowaniem do akwizycji i cyfrowej obróbki obrazu. Cost: 495 000 PLN
- Specjalistyczne oprogramowanie do rekonstrukcji obrazu mikroskopowego 3-D i 4-D. Cost: 160 000 PLN
- Tablet Aspire P3-171.
- Termocykler do PCR z blokiem na 96 probówek 0,2 ml / płytkę PCR z 96 studzienkami i gradientem temperatury. Cost: 35 000 PLN
- Termocykler do PCR z trzema niezależnymi blokami.
- Wirówka z rotorem na probówki 5 ml.
Information in the final report
Publication in academic press/journals (19)
-
Ribosomal DNA loci derived from Brachypodium stacei are switched off for major parts of the life cycle of Brachypodium hybridum
- Authors:
- Natalia Borowska-Zuchowska, Ewa Robaszkiewicz, Elzbieta Wolny, Alexander Betekhtin, Robert Hasterok
- Academic press:
- Journal of Experimental Botany (year: 2019, volume: 70, pages: 805–815), Publisher: Oxford University Press on behalf of the Society for Experimental Biology
- Status:
- Published
- DOI:
- 10.1093/jxb/ery425 – link to the publication
-
Dissecting the chromosomal composition of mutagen-induced micronuclei in Brachypodium distachyon using multicolour FISH
- Authors:
- Arita Kus, Jolanta Kwasniewska, Joanna Szymanowska-Pulka, Robert Hasterok
- Academic press:
- Annals of Botany (year: 2018, volume: 122, pages: 1161–1171), Publisher: Oxford University Press
- Status:
- Published
- DOI:
- 10.1093/aob/mcy115 – link to the publication
-
In situ analysis of epigenetic modifications in the chromatin of Brachypodium distachyon embryos
- Authors:
- Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Daria Kroczek, Robert Hasterok
- Academic press:
- Plant Signaling & Behavior (year: 2015, volume: 10, pages: e1011948 (1-2)), Publisher: Taylor & Francis Group
- Status:
- Published
- DOI:
- 10.1080/15592324.2015.1011948 – link to the publication
-
Reconstructing the evolution of Brachypodium genomes using comparative chromosome painting
- Authors:
- Alexander Betekhtin, Glyn Jenkins, Robert Hasterok
- Academic press:
- PLOS ONE (year: 2014, volume: 9, pages: e115108 (1-26)), Publisher: Public Library od Science
- Status:
- Published
- DOI:
- 10.1371/journal.pone.0115108 – link to the publication
-
Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination
- Authors:
- Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Robert Hasterok
- Academic press:
- PLOS ONE (year: 2014, volume: 9, pages: e101246 (1-10)), Publisher: Public Library of Science
- Status:
- Published
- DOI:
- 10.1371/journal.pone.0101246 – link to the publication
-
Cytomolecular analysis of ribosomal DNA evolution in a natural allotetraploid Brachypodium hybridum and its putative ancestors — dissecting complex repetitive structure of intergenic spacers
- Authors:
- Natalia Borowska-Zuchowska, Miroslaw Kwasniewski, Robert Hasterok
- Academic press:
- Frontiers in Plant Science (year: 2016, volume: 7, pages: 1499 (1-15)), Publisher: Frontiers
- Status:
- Published
- DOI:
- 10.3389/fpls.2016.01499 – link to the publication
-
Chromosome territory modeller and viewer
- Authors:
- Magdalena A. Tkacz, Kornel Chrominski, Dominika Idziak-Helmcke, Ewa Robaszkiewicz, Robert Hasterok
- Academic press:
- PLOS ONE (year: 2016, volume: 11, pages: e0160303 (1-20)), Publisher: Public Library od Science
- Status:
- Published
- DOI:
- 10.1371/journal. – link to the publication
-
Spatial distribution of centromeres and telomeres at interphase varies among Brachypodium species
- Authors:
- Dominika Idziak, Ewa Robaszkiewicz, Robert Hasterok
- Academic press:
- Journal of Experimental Botany (year: 2015, volume: 66, pages: 6623-6634), Publisher: Oxford Journals
- Status:
- Published
-
Insight into the karyotype evolution of Brachypodium species using comparative chromosome barcoding
- Authors:
- Dominika Idziak, Iwona Hazuka, Beata Poliwczak, Anna Wiszynska, Elzbieta Wolny, Robert Hasterok
- Academic press:
- PLOS ONE (year: 2014, volume: 9, pages: e93503 (1-10)), Publisher: Public Library od Science
- Status:
- Published
- DOI:
- 10.1371/journal.pone.0093503 – link to the publication
-
Epigenetics of the preferential silencing of Brachypodium stacei-originated 35S rDNA loci in the allotetraploid grass Brachypodium hybridum
- Authors:
- Natalia Borowska-Zuchowska, Robert Hasterok
- Academic press:
- Scientific Reports (year: 2017, volume: 7, pages: 5260 (1-12)), Publisher: Nature Publishing Group
- Status:
- Published
- DOI:
- 10.1038/s41598-017-05413-x – link to the publication
-
Chromosome identification and reconstruction of evolutionary rearrangements in Brachypodium distachyon, B. stacei and B. hybridum
- Authors:
- Joanna Lusinska, Joanna Majka, Alexander Betekhtin, Karolina Susek, Elzbieta Wolny, Robert Hasterok
- Academic press:
- Annals of Botany (year: 2018, volume: 122, pages: 445–459), Publisher: Oxford University Press
- Status:
- Published
- DOI:
- 10.1093/aob/mcy086 – link to the publication
-
The arrangement of Brachypodium distachyon chromosomes in interphase nuclei
- Authors:
- Ewa Robaszkiewicz, Dominika Idziak-Helmcke, Magdalena A. Tkacz, Kornel Chrominski, Robert Hasterok
- Academic press:
- Journal of Experimental Botany (year: 2016, volume: 67, pages: 5571–5583), Publisher: Oxford Journals
- Status:
- Published
- DOI:
- 10.1093/jxb/erw325 – link to the publication
-
Karyotyping of Brachypodium pinnatum (2n = 18) chromosomes using cross-species BAC–FISH
- Authors:
- Elzbieta Wolny, Wojciech Fidyk, Robert Hasterok
- Academic press:
- Genome (year: 2013, volume: 56, pages: 239-243), Publisher: NRC Research Press Journal
- Status:
- Published
- DOI:
- 10.1139/gen-2013-001 – link to the publication
-
Brachypodium distachyon - a useful model in the qualification of mutagen-induced micronuclei using multicolor FISH
- Authors:
- Arita Kus, Jolanta Kwasniewska, Robert Hasterok
- Academic press:
- PLOS ONE (year: 2017, volume: 12, pages: e0170618-1-e0170618-13), Publisher: Public Library od Science
- Status:
- Published
- DOI:
- 10.1371/journal.pone.0170618 – link to the publication
-
Histone H3 and H4 acetylation patterns are more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination
- Authors:
- Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Daria Kroczek, Robert Hasterok
- Academic press:
- Protoplasma (year: 2017, volume: 254, pages: 2045–2052), Publisher: Springer Wien
- Status:
- Published
- DOI:
- 10.1007/s00709-017-1088-x – link to the publication
-
Detecting Brachypodium distachyon chromosomes Bd4 and Bd5 in MH- and X-ray-induced micronuclei using mcFISH
- Authors:
- Arita Kus, Joanna Szymanowska-Pułka, Jolanta Kwasniewska, Robert Hasterok
- Academic press:
- International Journal of Molecular Sciences (year: 2019, volume: 20, pages: 2848 (1-13)), Publisher: Multidisciplinary Digital Publishing Institute
- Status:
- Published
- DOI:
- 10.3390/ijms20112848 – link to the publication
-
Update on genomics and basic biology of Brachypodium
- Authors:
- International Brachypodium Initiative (IBI): Pilar Catalan, Boulos Chalhoub, Vincent Chochois, David F. Garvin, Robert Hasterok, Antonio J. Manzaneda, Luis A.J. Mur, Nicola Pecchioni, Søren K. Rasmussen, John P. Vogel, Aline Voxeur
- Academic press:
- Trends in Plant Science (year: 2014, volume: 19, pages: 414-418), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.tplants.2014.05.002 – link to the publication
-
Comparatively barcoded chromosomes of Brachypodium perennials tell the story of their karyotype structure and evolution
- Authors:
- Joanna Lusinska, Alexander Betekhtin, Diana Lopez-Alvarez, Pilar Catalan, Glyn Jenkins, Elzbieta Wolny, Robert Hasterok
- Academic press:
- International Journal of Molecular Sciences (year: 2019, volume: 20, pages: 5557 (1-19)), Publisher: Multidisciplinary Digital Publishing Institute
- Status:
- Published
- DOI:
- 10.3390/ijms20225557 – link to the publication
-
Gradual polyploid genome evolution revealed by a pan-genomic analysis of Brachypodium hybridum and its diploid progenitors
- Authors:
- Sean P. Gordon, Bruno Contreras-Moreira, Joshua J. Levy, Armin Djamei, Angelika Czedik-Eysenberg, Virginia S. Tartaglio, Adam Session, Joel Martin, Amy Cartwright, Andrew Katz, Vasanth R. Singan, Eugene Goltsman, Kerrie Barry, Vinh Ha Dinh-Thi, Boulos Chalhoub, Antonio Diaz-Perez, Ruben Sancho, Joanna Lusinska, Elzbieta Wolny, Candida Nibau, John H. Doonan, Luis Mur, Chris Plott, Jerry Jenkins, Samuel P. Hazen, Scott J. Lee, Shenquaing Shu, David Goodstein, Daniel Rokhsar, Jeremy Schmutz, Robert Hasterok, Pilar Catalan, John P. Vogel
- Status:
- Accepted for publication
Book publications / chapters in book publications (2)
-
Molecular cytogenetics in the genus Brachypodium
- Authors:
- Robert Hasterok, Alexander Betekhtin, Natalia Borowska-Zuchowska, Agnieszka Braszewska-Zalewska, Dominika Idziak-Helmcke, Ewa Robaszkiewicz, Elzbieta Wolny
- Book:
- Genetics and Genomics of Brachypodium (Plant Genetics and Genomics: Crops Models) (year: 2015, volume: 18, pages: 39-54), Publisher: Springer International Publishing Switzerland
- Status:
- Published
-
Methods for cytogenetic chromosome barcoding and chromosome iainting in Brachypodium distachyon and its relative species
- Authors:
- Dominika Idziak-Helmcke, Alexander Betekhtin
- Book:
- Brachypodium Genomics (Methods and Protocols: Methods in Molecular Biology) (year: 2018, volume: 1667, pages: 46041), Publisher: Springer Science+Business Media LLC
- Status:
- Published