Plant nuclear genome - its structure, dynamics and evolution from the cytomolecular perspective

2012/04/A/NZ3/00572

Keywords

Descriptors

  • NZ2_5: Cytogenetyka
  • NZ3_1: Biologia komórki
  • NZ1_1: Biologia molekularna

Panel

NZ3 – Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution

UNIWERSYTET ŚLĄSKI, Wydział Biologii i Ochrony Środowiska

Voivodeship: śląskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Robert Hasterok

Number of co-investigators in the project: 13

Call

MAESTRO 2 – announced on 2011-12-15

Amount awarded
2 691 135 PLN
Project start date
2012-08-21
Project end date
2018-05-20
Project duration
69 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Homogenizator ultradźwiękowy przystosowany do próbek o małej objętości w zamkniętych probówkach. Cost: 85 000 PLN
  2. Laptop Asus G750JH-T4100H.
  3. Mikroskop epifluorescencyjny szerokiego pola wyposażony w ośmiopozycyjny uchwyt filtrów wraz z zestawami wąskopasmowych filtrów fluorescencyjnych oraz kamerą cyfrową wysokiej czułości i oprogramowaniem do akwizycji i cyfrowej obróbki obrazu. Cost: 495 000 PLN
  4. Specjalistyczne oprogramowanie do rekonstrukcji obrazu mikroskopowego 3-D i 4-D. Cost: 160 000 PLN
  5. Tablet Aspire P3-171.
  6. Termocykler do PCR z blokiem na 96 probówek 0,2 ml / płytkę PCR z 96 studzienkami i gradientem temperatury. Cost: 35 000 PLN
  7. Termocykler do PCR z trzema niezależnymi blokami.
  8. Wirówka z rotorem na probówki 5 ml.

Information in the final report

Publication in academic press/journals (19)

  1. Ribosomal DNA loci derived from Brachypodium stacei are switched off for major parts of the life cycle of Brachypodium hybridum

    Authors:
    Natalia Borowska-Zuchowska, Ewa Robaszkiewicz, Elzbieta Wolny, Alexander Betekhtin, Robert Hasterok
    Academic press:
    Journal of Experimental Botany (year: 2019, volume: 70, pages: 805–815), Publisher: Oxford University Press on behalf of the Society for Experimental Biology
    Status:
    Published
    DOI:
    10.1093/jxb/ery425 – link to the publication
  2. Dissecting the chromosomal composition of mutagen-induced micronuclei in Brachypodium distachyon using multicolour FISH

    Authors:
    Arita Kus, Jolanta Kwasniewska, Joanna Szymanowska-Pulka, Robert Hasterok
    Academic press:
    Annals of Botany (year: 2018, volume: 122, pages: 1161–1171), Publisher: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/aob/mcy115 – link to the publication
  3. In situ analysis of epigenetic modifications in the chromatin of Brachypodium distachyon embryos

    Authors:
    Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Daria Kroczek, Robert Hasterok
    Academic press:
    Plant Signaling & Behavior (year: 2015, volume: 10, pages: e1011948 (1-2)), Publisher: Taylor & Francis Group
    Status:
    Published
    DOI:
    10.1080/15592324.2015.1011948 – link to the publication
  4. Reconstructing the evolution of Brachypodium genomes using comparative chromosome painting

    Authors:
    Alexander Betekhtin, Glyn Jenkins, Robert Hasterok
    Academic press:
    PLOS ONE (year: 2014, volume: 9, pages: e115108 (1-26)), Publisher: Public Library od Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0115108 – link to the publication
  5. Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination

    Authors:
    Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Robert Hasterok
    Academic press:
    PLOS ONE (year: 2014, volume: 9, pages: e101246 (1-10)), Publisher: Public Library of Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0101246 – link to the publication
  6. Cytomolecular analysis of ribosomal DNA evolution in a natural allotetraploid Brachypodium hybridum and its putative ancestors — dissecting complex repetitive structure of intergenic spacers

    Authors:
    Natalia Borowska-Zuchowska, Miroslaw Kwasniewski, Robert Hasterok
    Academic press:
    Frontiers in Plant Science (year: 2016, volume: 7, pages: 1499 (1-15)), Publisher: Frontiers
    Status:
    Published
    DOI:
    10.3389/fpls.2016.01499 – link to the publication
  7. Chromosome territory modeller and viewer

    Authors:
    Magdalena A. Tkacz, Kornel Chrominski, Dominika Idziak-Helmcke, Ewa Robaszkiewicz, Robert Hasterok
    Academic press:
    PLOS ONE (year: 2016, volume: 11, pages: e0160303 (1-20)), Publisher: Public Library od Science
    Status:
    Published
    DOI:
    10.1371/journal. – link to the publication
  8. Spatial distribution of centromeres and telomeres at interphase varies among Brachypodium species

    Authors:
    Dominika Idziak, Ewa Robaszkiewicz, Robert Hasterok
    Academic press:
    Journal of Experimental Botany (year: 2015, volume: 66, pages: 6623-6634), Publisher: Oxford Journals
    Status:
    Published
  9. Insight into the karyotype evolution of Brachypodium species using comparative chromosome barcoding

    Authors:
    Dominika Idziak, Iwona Hazuka, Beata Poliwczak, Anna Wiszynska, Elzbieta Wolny, Robert Hasterok
    Academic press:
    PLOS ONE (year: 2014, volume: 9, pages: e93503 (1-10)), Publisher: Public Library od Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0093503 – link to the publication
  10. Epigenetics of the preferential silencing of Brachypodium stacei-originated 35S rDNA loci in the allotetraploid grass Brachypodium hybridum

    Authors:
    Natalia Borowska-Zuchowska, Robert Hasterok
    Academic press:
    Scientific Reports (year: 2017, volume: 7, pages: 5260 (1-12)), Publisher: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-017-05413-x – link to the publication
  11. Chromosome identification and reconstruction of evolutionary rearrangements in Brachypodium distachyon, B. stacei and B. hybridum

    Authors:
    Joanna Lusinska, Joanna Majka, Alexander Betekhtin, Karolina Susek, Elzbieta Wolny, Robert Hasterok
    Academic press:
    Annals of Botany (year: 2018, volume: 122, pages: 445–459), Publisher: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/aob/mcy086 – link to the publication
  12. The arrangement of Brachypodium distachyon chromosomes in interphase nuclei

    Authors:
    Ewa Robaszkiewicz, Dominika Idziak-Helmcke, Magdalena A. Tkacz, Kornel Chrominski, Robert Hasterok
    Academic press:
    Journal of Experimental Botany (year: 2016, volume: 67, pages: 5571–5583), Publisher: Oxford Journals
    Status:
    Published
    DOI:
    10.1093/jxb/erw325 – link to the publication
  13. Karyotyping of Brachypodium pinnatum (2n = 18) chromosomes using cross-species BAC–FISH

    Authors:
    Elzbieta Wolny, Wojciech Fidyk, Robert Hasterok
    Academic press:
    Genome (year: 2013, volume: 56, pages: 239-243), Publisher: NRC Research Press Journal
    Status:
    Published
    DOI:
    10.1139/gen-2013-001 – link to the publication
  14. Brachypodium distachyon - a useful model in the qualification of mutagen-induced micronuclei using multicolor FISH

    Authors:
    Arita Kus, Jolanta Kwasniewska, Robert Hasterok
    Academic press:
    PLOS ONE (year: 2017, volume: 12, pages: e0170618-1-e0170618-13), Publisher: Public Library od Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0170618 – link to the publication
  15. Histone H3 and H4 acetylation patterns are more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination

    Authors:
    Elzbieta Wolny, Agnieszka Braszewska-Zalewska, Daria Kroczek, Robert Hasterok
    Academic press:
    Protoplasma (year: 2017, volume: 254, pages: 2045–2052), Publisher: Springer Wien
    Status:
    Published
    DOI:
    10.1007/s00709-017-1088-x – link to the publication
  16. Detecting Brachypodium distachyon chromosomes Bd4 and Bd5 in MH- and X-ray-induced micronuclei using mcFISH

    Authors:
    Arita Kus, Joanna Szymanowska-Pułka, Jolanta Kwasniewska, Robert Hasterok
    Academic press:
    International Journal of Molecular Sciences (year: 2019, volume: 20, pages: 2848 (1-13)), Publisher: Multidisciplinary Digital Publishing Institute
    Status:
    Published
    DOI:
    10.3390/ijms20112848 – link to the publication
  17. Update on genomics and basic biology of Brachypodium

    Authors:
    International Brachypodium Initiative (IBI): Pilar Catalan, Boulos Chalhoub, Vincent Chochois, David F. Garvin, Robert Hasterok, Antonio J. Manzaneda, Luis A.J. Mur, Nicola Pecchioni, Søren K. Rasmussen, John P. Vogel, Aline Voxeur
    Academic press:
    Trends in Plant Science (year: 2014, volume: 19, pages: 414-418), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.tplants.2014.05.002 – link to the publication
  18. Comparatively barcoded chromosomes of Brachypodium perennials tell the story of their karyotype structure and evolution

    Authors:
    Joanna Lusinska, Alexander Betekhtin, Diana Lopez-Alvarez, Pilar Catalan, Glyn Jenkins, Elzbieta Wolny, Robert Hasterok
    Academic press:
    International Journal of Molecular Sciences (year: 2019, volume: 20, pages: 5557 (1-19)), Publisher: Multidisciplinary Digital Publishing Institute
    Status:
    Published
    DOI:
    10.3390/ijms20225557 – link to the publication
  19. Gradual polyploid genome evolution revealed by a pan-genomic analysis of Brachypodium hybridum and its diploid progenitors

    Authors:
    Sean P. Gordon, Bruno Contreras-Moreira, Joshua J. Levy, Armin Djamei, Angelika Czedik-Eysenberg, Virginia S. Tartaglio, Adam Session, Joel Martin, Amy Cartwright, Andrew Katz, Vasanth R. Singan, Eugene Goltsman, Kerrie Barry, Vinh Ha Dinh-Thi, Boulos Chalhoub, Antonio Diaz-Perez, Ruben Sancho, Joanna Lusinska, Elzbieta Wolny, Candida Nibau, John H. Doonan, Luis Mur, Chris Plott, Jerry Jenkins, Samuel P. Hazen, Scott J. Lee, Shenquaing Shu, David Goodstein, Daniel Rokhsar, Jeremy Schmutz, Robert Hasterok, Pilar Catalan, John P. Vogel
    Status:
    Accepted for publication

Book publications / chapters in book publications (2)

  1. Molecular cytogenetics in the genus Brachypodium

    Authors:
    Robert Hasterok, Alexander Betekhtin, Natalia Borowska-Zuchowska, Agnieszka Braszewska-Zalewska, Dominika Idziak-Helmcke, Ewa Robaszkiewicz, Elzbieta Wolny
    Book:
    Genetics and Genomics of Brachypodium (Plant Genetics and Genomics: Crops Models) (year: 2015, volume: 18, pages: 39-54), Publisher: Springer International Publishing Switzerland
    Status:
    Published
  2. Methods for cytogenetic chromosome barcoding and chromosome iainting in Brachypodium distachyon and its relative species

    Authors:
    Dominika Idziak-Helmcke, Alexander Betekhtin
    Book:
    Brachypodium Genomics (Methods and Protocols: Methods in Molecular Biology) (year: 2018, volume: 1667, pages: 46041), Publisher: Springer Science+Business Media LLC
    Status:
    Published

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Graphic design and implementation: Jakub Piotrowski