Oligosaccharide structures in human seminal plasma glycoproteins: application of mass spectrometry and lectin-binding for evaluation of the impact of aberrant glycans on male infertility

2011/03/B/NZ5/05652

Keywords

Descriptors

  • NZ5_1: Etiologia chorób człowieka
  • NZ1_2: Biochemia

Panel

NZ5 – Human and animal noninfectious diseases: etiology, mechanisms, diagnosis and treatment of diseases, poisonings and injuries (without neurological diseases)

Host institution

Uniwersytet Medyczny im. Piastów Śląskich we Wrocławiu, Wydział Farmaceutyczny z Oddziałem Analiutyki Medycznej

Voivodeship: dolnośląskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Andrzej Wincenty Gamian

Number of co-investigators in the project: 8

Call

OPUS 2 – announced on 2011-09-15

Amount awarded
322 400 PLN
Project start date
2012-08-22
Project end date
2015-08-21
Project duration
36 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Aparat do elektrotransferu Hoefer.
  2. Koncentrator próżniowy Eppendorf.
  3. System do elektroforezy pionowek Hoefer SE 600. Cost: 10 200 PLN
  4. System dokumentacji żeli i blotów G Box Syngene. Cost: 72 000 PLN
  5. Zestaw kolumn HPLC do analizy glikanów. Cost: 35 000 PLN
  6. komora laminarna Alpina.

Information in the final report

Publication in academic press/journals (3)

  1. Terminal mannose residues in seminal plasma glycoproteins of infertile men

    Authors:
    Olejnik B, Jarząb A, Kratz EM, Zimmer M, Gamian A, Ferens-Sieczkowska M
    Academic press:
    International Journal of Molecular Sciences (year: 2015, volume: 16(7), pages: 14933-14950), Publisher: MDPI AG
    Status:
    Published
    DOI:
    10.3390/ijms160714933 – link to the publication
  2. Preliminary MALDI-TOF-MS analysis of seminal plasma N-glycome of infertile men

    Authors:
    Anna Kałuża; Anna Jarząb; Andrzej Gamian; Ewa M. Kratz; Mariusz Zimmer; Mirosława Ferens-Sieczkowska
    Academic press:
    Carbohydrate Research (year: 2016, volume: 435, pages: 19-25), Publisher: Elsevier BV
    Status:
    Published
    DOI:
    10.1016/j.carres.2016.09.009 – link to the publication
  3. The Analysis of Sialylation, N-Glycan Branching, and Expression of O-Glycans in Seminal Plasma of Infertile Men

    Authors:
    Ewa M. Kratz, Anna Kałuża, Mariusz Zimmer, and Mirosława Ferens-Sieczkowska
    Academic press:
    Disease Markers (year: 2015, volume: 2015, pages: ID 941871), Publisher: Hindawi Publishing Corporation
    Status:
    Published
    DOI:
    10.1155/2015/941871 – link to the publication

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