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Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Combinatorial library of fluorogenic substrates containing natural / unnatural amino acids for substrate specificity profiling of caspases.

2011/03/B/ST5/01048

Keywords:

caspase substrate combinatorial chemistry

Descriptors:

  • ST5_16:

Panel:

ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution :

Politechnika Wrocławska, Wydział Chemiczny

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Marcin Drąg 

Number of co-investigators in the project: 3

Call: OPUS 2 - announced on 2011-09-15

Amount awarded: 830 000 PLN

Project start date (Y-m-d): 2012-08-29

Project end date (Y-m-d): 2015-08-28

Project duration:: 36 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Wyparka rotacyjna z pompą mebranową (19 980 PLN)
  2. Zestaw do chromatografii HPLC i komputer sterujący.
  3. Liofilizator (50 000 PLN)
  4. Spektorfluorymetr płytkowy.
  5. Robot do syntezy peptydów na nośniku stałym (280 000 PLN)
  6. Zamrażarka.

Information in the final report

  • Publication in academic press/journals (5)
  • Articles in post-conference publications (3)
  • Book publications / chapters in book publications (1)
  1. Unnatural amino acids increase sensitivity and provide for the design of highly selective caspase substrates
    Authors:
    Marcin Poręba, Paulina Kasperkiewicz, Scott J. Snipas, Domenico Fasci, Guy S. Salvesen, Marcin Drąg
    Academic press:
    Cell Death and Differentiation (rok: 2014, tom: 9, strony: 1482-92), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/cdd.2014.64. - link to the publication
  2. Caspase substrates and inhibitors
    Authors:
    Marcin Poręba, Aleksandra Stróżyk, Guy S. Salvesen, Marcin Drąg
    Academic press:
    Cold Spring Harbor Perspectives in Biology (rok: 2013, tom: 45505, strony: 45311), Wydawca: Cold Spring Harbor Publications
    Status:
    Published
    DOI:
    10.1101/cshperspect.a008680 - link to the publication
  3. Substrate Specificity and Possible Heterologous Targets of Phytaspase, a Plant Cell Death Protease
    Authors:
    Raisa A. Galiullina, Paulina Kasperkiewicz, Nina V. Chichkova, Aleksandra Szalek, Marina V. Serebryakova, Marcin Poreba, Marcin Drag, and Andrey B. Vartapetian
    Academic press:
    The Journal of Biological Chemistry (rok: 2015, tom: 290, strony: 24806–24815), Wydawca: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
    Status:
    Published
    DOI:
    10.1074/jbc.M115.675819 - link to the publication
  4. Small Molecule Active Site Directed Tools for Studying Human Caspases
    Authors:
    Marcin Poręba, Aleksandra Szalek, Paulina Kasperkiewicz, Wioletta Rut, Guy S. Salvesen, Marcin Drąg
    Academic press:
    Chemical Reviews (rok: 2015, tom: 115 (22), strony: 12546–12629), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.chemrev.5b00434 - link to the publication
  5. Recent advances and concepts in substrate specificity determination of proteases using tailored libraries of fluorogenic substrates with unnatural amino acids.
    Authors:
    Wioletta Rut , Paulina Kasperkiewicz, Anna Byzia, Marcin Poręba, Katarzyna Groborz, Marcin Drag
    Academic press:
    Biological Chemistry (rok: 2015, tom: 396, strony: 329–337), Wydawca: De Gruyter
    Status:
    Published
    DOI:
    10.1515/hsz-2014-0315 - link to the publication
  1. New and Sensitive Donor/Acceptor Pair for Internally Quenched Fluorescent Peptide Substrates of Proteases
    Authors:
    Aleksandra Stróżyk, Marcin Poręba, Wioletta Rut, Paulina Kasperkiewicz, Scott Snipas, Guy S. Salvesen, Christopher Overall, Marko Mihelic, Dusan Turk, Marcin Drąg
    Conference:
    22nd Polish Peptide Symposium (rok: 2013, ), Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
    Data:
    konferencja 1-5 września
    Status:
    Published
  2. Unnatural amino acids provide for the design of highly selective substrates for caspases and legumain
    Authors:
    23.Marcin Poręba, Paulina Kasperkiewicz, Wioletta Rut, Scott J. Snipas, Dusan Turk, Guy S. Salvesen, Marcin Drąg
    Conference:
    Pacific Coast Protease Spring School (rok: 2014, ), Wydawca: książka abstraktowa z konferencji
    Data:
    konferencja 29.04.-02.05.2014
    Status:
    Published
  3. The use of unnatural amino acids in peptide fluorogenic substrates reveals unexpected active site preferences of human caspases and legumain"
    Authors:
    Marcin Poręba, Paulina Kasperkiewicz, Wioletta Rut, Scott Snipas, Marko Mihelic, Dusan Turk, Guy S. Salvesen, Marcin Drąg
    Conference:
    THE 8TH GENERAL MEETING OF THE INTERNATIONAL PROTEOLYSIS SOCIETY (rok: 2013, ), Wydawca: INTERNATIONAL PROTEOLYSIS SOCIETY
    Data:
    konferencja 20 - 24 października 2013
    Status:
    Published
  1. Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity
    Authors:
    Marcin Poręba, Aleksandra Szalek, Paulina Kasperkiewicz, Marcin Drąg
    Book:
    Methods in Molecular Biology (rok: 2014, tom: 1133, strony: 41-59), Wydawca: Clifton, N.J. : Humana Press
    Status:
    Published
    DOI:
    link to the publication

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