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Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Mesenchymal stem cells of pigs, their characteristic and utility in bovine in vitro embryo production and porcine somatic cell nuclear transfer

2011/03/D/NZ9/05537

Keywords:

mesenchymal stem cells oocyte embryo IVF SCNT interspecies SCNT

Descriptors:

  • NZ9_2: Animal production
  • NZ9_12: Conservation of genetic resources

Panel:

NZ9 - Fundamentals of applied life sciences and biotechnology: agricultural, forestry, horticulture, animal production and fishery, food and nutrition sciences, industrial biosciences, environmental biotechnology and remediation

Host institution :

Instytut Zootechniki - Państwowy Instytut Badawczy

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Jolanta Opiela 

Number of co-investigators in the project: 8

Call: SONATA 2 - announced on 2011-09-15

Amount awarded: 669 750 PLN

Project start date (Y-m-d): 2012-09-12

Project end date (Y-m-d): 2016-02-11

Project duration:: 41 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. odwrócony mikroskop epifluorescencyjny firmy Nicon (270 000 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  • Articles in post-conference publications (8)
  1. DNA Aneuploidy in Porcine Bone Marrow–Derived Mesenchymal Stem Cells Undergoing Osteogenic and Adipogenic In Vitro Differentiation
    Authors:
    Opiela J, Samiec M., Bochenek M, Lipinski D, Romanek J, Wilczek P
    Academic press:
    Cellular Reprogramming (rok: 2013, tom: 15 n5, strony: 425-34), Wydawca: Mary Ann Liebert Inc.
    Status:
    Published
    DOI:
    10.1089/cell.2012.0099 - link to the publication
  2. Characterization of mesenchymal stem cells and their application in experimental embryology.
    Authors:
    Opiela J, Samiec M
    Academic press:
    Pol. J Vet Sci (rok: 2013, tom: v. 16 n.3., strony: 593-599), Wydawca: Polish Academy of Sciences Committee of Veterinary Sciences and University of Warmia and Mazury in Olsztyn
    Status:
    Published
    DOI:
    http://www.ncbi.nlm.nih.gov/pubmed/24195301?report=abstract - link to the publication
  3. In vitro development and cytological quality of inter-species (porcine/bovine) cloned embryos are affected by trichostatin A-dependent epigenomic modulation of adult mesenchymal stem cells
    Authors:
    Opiela J, Samiec M, Romanek J
    Academic press:
    Theriogenology (rok: 2017, tom: 97, strony: 27-33), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.theriogenology.2017.04.022 - link to the publication
  4. Trichostatin A-mediated epigenetic transformation of adult bone marrow-derived mesenchymal stem cells biases the in vitro developmental capability, quality and pluripotency extent of porcine cloned embryos
    Authors:
    Samiec M, Opiela J, Lipiński D, Romanek J
    Academic press:
    BioMed Research International (rok: 2015, tom: volume 2015, strony: 13 pages), Wydawca: Hindawi
    Status:
    Published
    DOI:
    10.1155/2015/814686. - link to the publication
  1. Different culture media affect growth rate and morphology of porcine bone marrow-derived mesenchymal stromal cells- the preliminary results of media screening suitable for both porcine MSCs and bovine embryo in vitro culture
    Authors:
    Opiela J, Bochenek M, Romanek J, Smorąg Z.
    Conference:
    5th Central European Congress of Life Sciences, EUROBIOTECH (rok: 2013, ), Wydawca: EUROBIOTECH
    Data:
    konferencja 8-11 X
    Status:
    Published
  2. The increased pluripotency of adult bone marrow-derived Mesenchymal Stem Cells undergoing Trichostatin A-dependent epigenomic transformation
    Authors:
    Opiela J, Lipiński D, Romanek J, Wieczorek J
    Conference:
    VII Zjazd Towarzystwa Biologii Rozrodu Program I streszczenia. s. 150 (rok: 2014, ), Wydawca: TBR
    Data:
    konferencja wrzesien
    Status:
    Published
  3. Abundance of blastocysts developed in vitro from porcine cloned embryos reconstructed with cell nuclei of adult bone marrow-descended mesenchynal stem cells.
    Authors:
    Samiec M, Opiela J, Koseniuk J.
    Conference:
    5th Central European Congress of Life Sciences, EUROBIOTECH (rok: 2013, ), Wydawca: Eurobiotech
    Data:
    konferencja 8-11 X
    Status:
    Published
  4. Kompetencje jąder szpikopochodnych komórek macierzystych poddanych modulacji epigenetycznej za pośrednictwem trichostatyny A, do pokierowania pozaustrojowym rozwojem klonalnych zarodków świni
    Authors:
    Samiec M, Opiela J
    Conference:
    VII Zjazd Towarzystwa Biologii Rozrodu (rok: 2014, ), Wydawca: TBR
    Data:
    konferencja wrzesien
    Status:
    Published
  5. The increased quality of cloned pig embryos reconstructed with cell nuclei of adult bone marrow-derived mesenchymal stem cells undergoing trichostatin a-dependent epigenomic transformation
    Authors:
    Opiela J, Samiec M, Romanek J
    Conference:
    30th Annual Meeting AETE (European Embryo Transfer Association) (rok: 2014, ), Wydawca: AETE
    Data:
    konferencja 12-13 wrzesnia
    Status:
    Published
  6. Highly efficient in vitro production of nuclear-transferred pig embryos using adult bone marrow-derived mesenchymal stem cells
    Authors:
    Samiec M, Opiela J
    Conference:
    29th AETE Conference in Istanbul, Turkey, (rok: 2013, ), Wydawca: AETE
    Data:
    konferencja 6-7 IX
    Status:
    Published
  7. High hydrostatic pressure impact on cryopreservated porcine mesenchymal stem cells quality- preliminary results
    Authors:
    Romanek J, Opiela J, Smorąg Z
    Conference:
    30th Annual Meeting AETE (European Embryo Transfer Association)- Dresden Germany (rok: 2014, ), Wydawca: AETE
    Data:
    konferencja 11-12 wrzesnia
    Status:
    Published
  8. Trichostatin A affects the competencies of adult mesenchymal stem cell nuclei to direct extracorporeal development of cloned pig embryos
    Authors:
    Samiec M, Opiela J
    Conference:
    30th Annual Meeting AETE (European Embryo Transfer Association)- Dresden Germany (rok: 2014, ), Wydawca: AETE
    Data:
    konferencja 11-12 wrzesnia
    Status:
    Published

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