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Correlation of dynamics of H1 linker histone with spatial architecture of chromatin in cell nuclei.

2011/01/B/NZ3/00609

Keywords

Descriptors

  • NZ3_2: Fizjologia komórki
  • ST6_4: Grafika i przetwarzanie obrazów

Panel

NZ3 – Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

Voivodeship: małopolskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Jerzy Dobrucki

Number of co-investigators in the project: 6

Call

OPUS 1 – announced on 2011-03-15

Amount awarded
590 900 PLN
Project start date
2011-12-07
Project end date
2015-06-06
Project duration
42 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Dysk twardy.
  2. Dyski do archiwizacji danych.
  3. Dyski zewnętrzne do archiwizacji danych. Cost: 2 992 PLN
  4. Filtry optyczne. Cost: 30 000 PLN
  5. Miernik światła. Cost: 11 549 PLN
  6. Miernik światła.
  7. Moniitor komputerowy. Cost: 6 686 PLN
  8. Monitor komputerowy.
  9. Program komputerowy do analizy obrazu Huygens (SVI).
  10. Program komputerowy do analizy obrazu Huygens (SVI) - II rata. Cost: 24 968 PLN
  11. Programy komputerowe. Cost: 80 000 PLN
  12. Serwer plikow NAS. Cost: 15 000 PLN
  13. Stacja robocza. Cost: 85 000 PLN
  14. butla CO2.
  15. statyw do butli ze sprezonym CO2.

Information in the final report

Publication in academic press/journals (15)

  1. Daunomycin, an antitumor DNA intercalator, influences histone-DNA interactions

    Authors:
    Wójcik K, Zarębski M, Cossarizza A, Dobrucki JW.
    Academic press:
    Cancer Biology and Therapy (year: 2013, volume: 14, pages: 823-832), Publisher: Landes Bioscience
    Status:
    Published
    DOI:
    10.4161/cbt.25328 – link to the publication

  2. Dynamics of the HP1β-PCNA-containing complexes in DNA replication and repair

    Authors:
    Trembecka-Lucas DO, Szczurek AT, Dobrucki JW.
    Academic press:
    Nucleus (year: 2013, volume: 4(1), pages: 74-82), Publisher: Landes Bioscience
    Status:
    Published
    DOI:
    10.4161/nucl.23683 – link to the publication

  3. Analysis of spatial correlations between patterns of DNA damage response and DNA replication in nuclei of cells subjected to replication stress or oxidative damage

    Authors:
    Bernas T, Berniak K, Rybak P, Zarębski M, Zhao H, Darzynkiewicz Z, Dobrucki JW.
    Academic press:
    Cytometry A (year: 2013, volume: 83(10), pages: 925-32), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22325 – link to the publication

  4. Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis

    Authors:
    Berniak K, Rybak P, Bernas T, Zarębski M, Biela E, Zhao H, Darzynkiewicz Z, Dobrucki JW.
    Academic press:
    Cytometry A (year: 2013, volume: 83(10), pages: 913-924), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22327 – link to the publication

  5. DNA damage signaling assessed in individual cells in relation to the cell cycle phase and induction of apoptosis.

    Authors:
    Darzynkiewicz Z, Zhao H, Halicka HD, Rybak P, Dobrucki J, Wlodkowic D.
    Academic press:
    Crit Rev Clin Lab Sci. (year: 2012, volume: Sep-Dec;49(5-6), pages: 199-217), Publisher: Informa Healthcare
    Status:
    Published
    DOI:
    10.3109/10408363.2012.738808 – link to the publication

  6. Real-time cell viability assays using a new anthracycline derivative DRAQ7®.

    Authors:
    Akagi J, Kordon M, Zhao H, Matuszek A, Dobrucki J, Errington R, Smith PJ, Takeda K, Darzynkiewicz Z, Wlodkowic D.
    Academic press:
    Cytometry A. 2013 Feb;83(2):227-34. (year: 2013, volume: 83(2), pages: 227-34), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22228 – link to the publication

  7. A heterochromatin protein 1 (HP1) dimer and a proliferating cell nuclear antigen (PCNA) protein interact in vivo and are parts of a multiprotein complex involved in DNA replication and DNA repair.

    Authors:
    Trembecka-Lucas DO, Dobrucki JW.
    Academic press:
    Cell Cycle (year: 2012, volume: 11(11), pages: 2170-5.), Publisher: Landes Bioscience
    Status:
    Published
    DOI:
    10.4161/cc.20673 – link to the publication

  8. Inducing local DNA damage by visible light to study chromatin repair

    Authors:
    Solarczyk KJ, Zarębski M, Dobrucki JW.
    Academic press:
    DNA Repair (Amst). (year: 2012, volume: 11(12), pages: 996-1002.), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.dnarep.2012.09.008 – link to the publication

  9. Photosensitized Damage Inflicted on Plasma Membranes of Live Cells by An Extracellular Generator of Singlet Oxygen-A Linear Dependence of A Lethal Dose on Light Intensity.

    Authors:
    Zarębski M, Kordon M, Dobrucki JW.
    Academic press:
    Photochemistry Photobiology (year: 2014, volume: 90, pages: 709-715), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1111/php.12216 – link to the publication

  10. UV-activated conversion of Hoechst 33258, DAPI, and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms

    Authors:
    Zurek-Biesiada D, Kędracka-Krok S, Dobrucki JW.
    Academic press:
    Cytometry A (year: 2013, volume: 83(5), pages: 441-51), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22260 – link to the publication

  11. Spatial heterogeneity of dynamics of H1 linker histone

    Authors:
    Bernas T, Brutkowski W, Zarębski M, Dobrucki J.
    Academic press:
    European Biophysical Journal (year: 2014, volume: 43(6-7), pages: 287-300), Publisher: Springer
    Status:
    Published

  12. UV-induced spectral shift and protonation of DNA fluorescent dye Hoechst 33258

    Authors:
    Żurek-Biesiada D, Waligórski P, Dobrucki JW.
    Academic press:
    Journal of Fluorescence (year: 2014, volume: 24(6), pages: 1791-801), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s10895-014-1468-y – link to the publication

  13. Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long-term tracking of acidic vesicles

    Authors:
    Pierzyńska-Mach A, Janowski PA, Dobrucki JW.
    Academic press:
    Cytometry Part A (year: 2014, volume: 85(8), pages: 729-37), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22495 – link to the publication

  14. Single molecule localization microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes

    Authors:
    Szczurek AT, Prakash K, Lee K-K, Żurek-Biesiada DJ, Best G, Hagmann M, Dobrucki JW, Cremer Ch, and Birk U
    Academic press:
    Nucleus (year: 2104, volume: 5(4), pages: 331-340), Publisher: Landes Bioscience
    Status:
    Published
    DOI:
    10.4161/nucl.29564 – link to the publication

  15. Activation of new replication foci under conditions of replication stress

    Authors:
    Rybak P, Waligórska A, Bujnowicz Ł, Hoang A, Dobrucki JW
    Status:
    Accepted for publication

Book publications / chapters in book publications (2)

  1. Kinetic viability assays using DRAQ7 probe

    Authors:
    Wlodkowic D, Akagi J, Dobrucki J, Errington R, Smith PJ, Takeda K, Darzynkiewicz Z
    Book:
    Curr Protoc Cytom. (year: 2013, volume: 2013, pages: 0-0), Publisher: Wiley
    Status:
    Published
    DOI:
    link to the publication

  2. Fluorescence Microscopy

    Authors:
    Dobrucki JW
    Book:
    Fluorescence Microscopy: From Principles to Biological Applications (year: 2013, volume: 1, pages: 97-142), Publisher: Wiley-Blackwell
    Status:
    Published
    DOI:
    link to the publication

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Graphic design and implementation: Jakub Piotrowski