Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Correlation of dynamics of H1 linker histone with spatial architecture of chromatin in cell nuclei.

2011/01/B/NZ3/00609

Keywords:

Histone H1 dynamics chromatin spatial architecture

Descriptors:

  • NZ3_2: Cell physiology
  • ST6_4: Formal methods, foundations of computer science, including theoretical computer science, quantum algorithms

Panel:

NZ3 - Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution :

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jerzy Dobrucki 

Number of co-investigators in the project: 6

Call: OPUS 1 - announced on 2011-03-15

Amount awarded: 590 900 PLN

Project start date (Y-m-d): 2011-12-07

Project end date (Y-m-d): 2015-06-06

Project duration:: 42 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Dyski do archiwizacji danych.
  2. Miernik światła (11 549 PLN)
  3. Program komputerowy do analizy obrazu Huygens (SVI).
  4. Program komputerowy do analizy obrazu Huygens (SVI) - II rata (24 968 PLN)
  5. statyw do butli ze sprezonym CO2.
  6. Dyski zewnętrzne do archiwizacji danych (2 992 PLN)
  7. Moniitor komputerowy (6 686 PLN)
  8. Monitor komputerowy.
  9. Programy komputerowe (80 000 PLN)
  10. Filtry optyczne (30 000 PLN)
  11. butla CO2.
  12. Dysk twardy.
  13. Miernik światła.
  14. Serwer plikow NAS (15 000 PLN)
  15. Stacja robocza (85 000 PLN)

Information in the final report

  • Publication in academic press/journals (15)
  • Book publications / chapters in book publications (2)
  1. DNA damage signaling assessed in individual cells in relation to the cell cycle phase and induction of apoptosis.
    Authors:
    Darzynkiewicz Z, Zhao H, Halicka HD, Rybak P, Dobrucki J, Wlodkowic D.
    Academic press:
    Crit Rev Clin Lab Sci. (rok: 2012, tom: Sep-Dec;49(5-6), strony: 199-217), Wydawca: Informa Healthcare
    Status:
    Published
    DOI:
    10.3109/10408363.2012.738808 - link to the publication
  2. Dynamics of the HP1β-PCNA-containing complexes in DNA replication and repair
    Authors:
    Trembecka-Lucas DO, Szczurek AT, Dobrucki JW.
    Academic press:
    Nucleus (rok: 2013, tom: 4(1), strony: 74-82), Wydawca: Landes Bioscience
    Status:
    Published
    DOI:
    10.4161/nucl.23683 - link to the publication
  3. Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long-term tracking of acidic vesicles
    Authors:
    Pierzyńska-Mach A, Janowski PA, Dobrucki JW.
    Academic press:
    Cytometry Part A (rok: 2014, tom: 85(8), strony: 729-37), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22495 - link to the publication
  4. Inducing local DNA damage by visible light to study chromatin repair
    Authors:
    Solarczyk KJ, Zarębski M, Dobrucki JW.
    Academic press:
    DNA Repair (Amst). (rok: 2012, tom: 11(12), strony: 996-1002.), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.dnarep.2012.09.008 - link to the publication
  5. Real-time cell viability assays using a new anthracycline derivative DRAQ7®.
    Authors:
    Akagi J, Kordon M, Zhao H, Matuszek A, Dobrucki J, Errington R, Smith PJ, Takeda K, Darzynkiewicz Z, Wlodkowic D.
    Academic press:
    Cytometry A. 2013 Feb;83(2):227-34. (rok: 2013, tom: 83(2), strony: 227-34), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22228 - link to the publication
  6. UV-induced spectral shift and protonation of DNA fluorescent dye Hoechst 33258
    Authors:
    Żurek-Biesiada D, Waligórski P, Dobrucki JW.
    Academic press:
    Journal of Fluorescence (rok: 2014, tom: 24(6), strony: 1791-801), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10895-014-1468-y - link to the publication
  7. Activation of new replication foci under conditions of replication stress
    Authors:
    Rybak P, Waligórska A, Bujnowicz Ł, Hoang A, Dobrucki JW
    Academic press:
    Cell Cycle (rok: 2015, ), Wydawca: Taylor and Francis
    Status:
    Accepted for publication
    DOI:
    brak - link to the publication
  8. Spatial heterogeneity of dynamics of H1 linker histone
    Authors:
    Bernas T, Brutkowski W, Zarębski M, Dobrucki J.
    Academic press:
    European Biophysical Journal (rok: 2014, tom: 43(6-7), strony: 287-300), Wydawca: Springer
    Status:
    Published
    DOI:
    0.1007/s00249-014-0962-0 - link to the publication
  9. Photosensitized Damage Inflicted on Plasma Membranes of Live Cells by An Extracellular Generator of Singlet Oxygen-A Linear Dependence of A Lethal Dose on Light Intensity.
    Authors:
    Zarębski M, Kordon M, Dobrucki JW.
    Academic press:
    Photochemistry Photobiology (rok: 2014, tom: 90, strony: 709-715), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1111/php.12216 - link to the publication
  10. A heterochromatin protein 1 (HP1) dimer and a proliferating cell nuclear antigen (PCNA) protein interact in vivo and are parts of a multiprotein complex involved in DNA replication and DNA repair.
    Authors:
    Trembecka-Lucas DO, Dobrucki JW.
    Academic press:
    Cell Cycle (rok: 2012, tom: 11(11), strony: 2170-5.), Wydawca: Landes Bioscience
    Status:
    Published
    DOI:
    10.4161/cc.20673 - link to the publication
  11. Analysis of spatial correlations between patterns of DNA damage response and DNA replication in nuclei of cells subjected to replication stress or oxidative damage
    Authors:
    Bernas T, Berniak K, Rybak P, Zarębski M, Zhao H, Darzynkiewicz Z, Dobrucki JW.
    Academic press:
    Cytometry A (rok: 2013, tom: 83(10), strony: 925-32), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22325 - link to the publication
  12. UV-activated conversion of Hoechst 33258, DAPI, and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms
    Authors:
    Zurek-Biesiada D, Kędracka-Krok S, Dobrucki JW.
    Academic press:
    Cytometry A (rok: 2013, tom: 83(5), strony: 441-51), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22260 - link to the publication
  13. Daunomycin, an antitumor DNA intercalator, influences histone-DNA interactions
    Authors:
    Wójcik K, Zarębski M, Cossarizza A, Dobrucki JW.
    Academic press:
    Cancer Biology and Therapy (rok: 2013, tom: 14, strony: 823-832), Wydawca: Landes Bioscience
    Status:
    Published
    DOI:
    10.4161/cbt.25328 - link to the publication
  14. Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis
    Authors:
    Berniak K, Rybak P, Bernas T, Zarębski M, Biela E, Zhao H, Darzynkiewicz Z, Dobrucki JW.
    Academic press:
    Cytometry A (rok: 2013, tom: 83(10), strony: 913-924), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.22327 - link to the publication
  15. Single molecule localization microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes
    Authors:
    Szczurek AT, Prakash K, Lee K-K, Żurek-Biesiada DJ, Best G, Hagmann M, Dobrucki JW, Cremer Ch, and Birk U
    Academic press:
    Nucleus (rok: 2104, tom: 5(4), strony: 331-340), Wydawca: Landes Bioscience
    Status:
    Published
    DOI:
    10.4161/nucl.29564 - link to the publication
  1. Fluorescence Microscopy
    Authors:
    Dobrucki JW
    Book:
    Fluorescence Microscopy: From Principles to Biological Applications (rok: 2013, tom: 1, strony: 97-142), Wydawca: Wiley-Blackwell
    Status:
    Published
    DOI:
    link to the publication
  2. Kinetic viability assays using DRAQ7 probe
    Authors:
    Wlodkowic D, Akagi J, Dobrucki J, Errington R, Smith PJ, Takeda K, Darzynkiewicz Z
    Book:
    Curr Protoc Cytom. (rok: 2013, tom: 2013, strony: 0-0), Wydawca: Wiley
    Status:
    Published
    DOI:
    link to the publication

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