Establishing of a pyrosequencing pipeline for DNA methylation analysis.
2011/01/D/NZ2/00095
Keywords
Descriptors
- NZ2_2: Genomika, transkryptomika i epigenomika
- NZ2_1: Genetyka molekularna
Panel
NZ2 – Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology
Host institution
Instytut Genetyki Człowieka PAN
Voivodeship: wielkopolskie
Principal investigator (from the host institution)
Number of co-investigators in the project: 3
Call
SONATA 1 – announced on 2011-03-15
- Amount awarded
- 890 500 PLN
- Project start date
- 2011-12-06
- Project end date
- 2014-12-05
- Project duration
- 36 months (the same as in the proposal)
- Project status
- Project settled
Equipment purchased [PL]
- Komora PCR, POLGEN. Cost: 8 000 PLN
- Lodówko-zamrażarka, EURO-NET. Cost: 2 000 PLN
- Miniwirówka, BIONOVO. Cost: 1 000 PLN
- System do sekwencjonowania, SYNGEN. Cost: 344 000 PLN
- Termocykler, BIO-RAD. Cost: 40 000 PLN
- Urządzenie do ciągłego monitoringu hodowli komórkowych. Cost: 69 467 PLN
- Vortex. Cost: 1 000 PLN
- Zestaw pipet oraz drobny sprzęt laboratoryjny. Cost: 14 000 PLN
Information in the final report
Publication in academic press/journals (2)
-
The PRDX2 gene is transcriptionally silenced and de novo methylated in Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma.
- Authors:
- Schneider M, Szaumkessel M, Richter J, Ammerpohl O, Hansmann ML, Küppers R, Siebert R, Giefing M.
- Academic press:
- Blood (year: 2014, volume: 123, pages: 3672-3674), Publisher: American Society of Hematology (United States)
- Status:
- Published
- DOI:
- 10.1182/blood-2014-02-553263 – link to the publication
-
Recurrent epigenetic silencing of the PTPRD tumor suppressor in laryngeal squamous cell carcinoma
- Authors:
- Szaumkessel M, Wojciechowska S, Janiszewska J, Zemke N, Byzia E, Kiwerska K, Kostrzewska-Poczekaj M, Ustaszewski A, Jarmuż-Szymczak M, Grenman R, Wierzbicka M, Bartochowska A, Szyfter K, and Giefing M.
- Status:
- Accepted for publication
Book publications / chapters in book publications (1)
-
Metoda pirosekwencjonowania w badaniu nowotworów.
- Authors:
- Byzia E, Giefing M.
- Book:
- Na Pograniczu Chemii i Biologii (year: 2014, volume: XXXII, pages: 205-223), Publisher: Wydawnictwo Naukowe Uniwersytetu Adama Mickiewicza w Poznaniu.
- Status:
- Published