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Molecular basis of amyloidogenesis - structure of conformational dynamics slected complexes of human prion protein PrPc with metal cations

2014/15/B/ST4/04839

Keywords

Descriptors

  • ST4_13: Chemia fizyczna układów biologicznych
  • ST4_3: Metody spektroskopowe i spektrometryczne
  • ST5_16: Chemia biologiczna

Panel

ST4 – Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution

Uniwersytet im. Adama Mickiewicza w Poznaniu, Wydział Fizyki

Voivodeship: wielkopolskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Maciej Leszek Kozak

Number of co-investigators in the project: 6

Call

OPUS 8 – announced on 2014-09-15

Amount awarded
919 820 PLN
Project start date
2015-09-21
Project end date
2019-09-20
Project duration
48 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Przystawka mikroskopowa AFM do mikroskopu optycznego. Cost: 255 000 PLN
  2. Zamrażarka laboratoryjna do próbek.. Cost: 6 485 PLN

Information in the final report

Publication in academic press/journals (6)

  1. Zn(II) binding causes interdomain changes in the structure and flexibility of the human prion protein

    Authors:
    Maciej Gielnik, Michal Taube, Lilia Zhukova , Igor Zhukov, Sebastian K.T.S. Wärmländer, Željko Svedružić, Wojciech M. Kwiatek, Astrid Graslund, Maciej Kozak
    Academic press:
    Scientific Reports (year: 2021, volume: 11, pages: Article number: 21703), Publisher: Springer - Nature Research
    Status:
    Published
    DOI:
    10.1038/s41598-021-00495-0 – link to the publication

  2. Preliminary results of human PrPC protein studied by spectroscopic techniques

    Authors:
    Michał Nowakowski, Joanna Czapla-Masztafiak, Maciej Kozak, Igor Zhukov, Lilia Zhukova, Jakub Szlachetko and Wojciech M. Kwiatek
    Academic press:
    Nuclear Instruments and Methods in Physics Research Section B (year: 2017, volume: 411, pages: 121-128), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.nimb.2017.06.022 – link to the publication

  3. Microchip Circulation Drastically Accelerates Amyloid Aggregation of 1−42 β‑amyloid Peptide from Felis catus

    Authors:
    Witold Gospodarczyk, Maciej Kozak
    Academic press:
    ACS Chememical Neuroscience (year: 2017, volume: 8, pages: 2558-2567), Publisher: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acschemneuro.7b00285 – link to the publication

  4. The engineered peptide construct NCAM1-Aβ inhibits aggregation of the human prion protein (PrP)

    Authors:
    Maciej Gielnik, Lilia Zhukova, Igor Zhukov, Astrid Gräslund, Maciej Kozak, Sebastian K.T.S. Wärmländer
    Academic press:
    Acta Biochimica Polonica (year: 2022, volume: 69, pages: 46027), Publisher: Polish Biochemical Society
    Status:
    Published
    DOI:
    10.18388/abp.2017_ – link to the publication

  5. Electronic properties of a PrPC–Cu(II) complex as a marker of 5-fold Cu(II) coordination

    Authors:
    Michał Nowakowski, Joann Czapla-Masztafiak, Igor Zhukov, Lilia Zhukova, Maciej Kozak and Wojciech M. Kwiatek
    Academic press:
    Metallomics (year: 2019, volume: 11, pages: art no. 632 (1-11)), Publisher: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C8MT00339D – link to the publication

  6. PrP (58–93) peptide from unstructured N-terminal domain of human prion protein forms amyloid-like fibrillar structures in the presence of Zn2+ ions

    Authors:
    Maciej Gielnik, Zuzanna Pietralik, Igor Zhukov, Aneta Szymańska, Wojciech M. Kwiatek , Maciej Kozak
    Academic press:
    RSC Advances (year: 2019, volume: 9, pages: 22211-22219), Publisher: Royal Socity of Chemistry
    Status:
    Published
    DOI:
    10.1039/C9RA01510H – link to the publication

Articles in post-conference publications (21)

  1. Structural studies of selected PrP peptides interactions of mutated octarepeat with zinc ions

    Authors:
    Joanna Wola, Michał Taube, Aneta Szymańska, Maciej Kozak
    Conference:
    The Joint Meeting of the Polish Synchrotron Radiation Society and SOLARIS Users (year: 2020, pages: 95), Publisher: Polish Synchrotron Radiation Society, Synchrotron Radiation in Natural Science 20 (2020)
    Date:
    conference 9 - 11 września 2020
    Status:
    Published

  2. Attempt to determine the structure of the huPrPC Cu( II)/Zn(II) complexes.

    Authors:
    Michał Nowakowski, Joanna Czapla- Masztafiak, Maciej Gielnik, Lilia Zhukova, Igor Zhukov, Maciej Kozak, Wojciech M. Kwiatek
    Conference:
    17th International Conference on X-ray Absorption Fine Structure (XAFS 2018) (year: 2018, pages: 46024), Publisher: Polish Synchrotron Radiation Society, International X-ray Absorption Society (IXAS)
    Date:
    conference 22-28 lipca
    Status:
    Published

  3. Influence of zinc ions on secondary structure of PrP (58-93) peptide variants containing octarepeat region

    Authors:
    Joanna Wolak, Aneta Szymańska, Maciej Kozak
    Conference:
    14th International School and Symposium on Synchrotron Radiation in Natural Science (ISSRNS'2019) (year: 2019, pages: 85), Publisher: Polskie Towarzystwo Promieniowania Synchrotronowego
    Date:
    conference 9-14 czerwca
    Status:
    Published

  4. Molecular basis of selected neurodegenerative disorders – SAXS, SANS and MD studies

    Authors:
    Maciej Kozak, Adriana Żyła, Joanna Wolak, Aneta Szymańska, Maciej Gielnik, Michał Taube, Igor Zhukov, Kosma Szutkowski, Michał Nowakowski, Wojciech M. Kwiatek, Alexander I. Kuklin
    Conference:
    The Fifth Korean-Polish Conference on Protein Folding: Theoretical and Experimental Approaches (year: 2019, pages: S04-1), Publisher: Korea Institute for Advanced Study
    Date:
    conference 16-18 września
    Status:
    Published

  5. Selected applications of small-angle X-ray and neutron scattering (SAXS/ SANS) in studies of complexes and flexible protein structures

    Authors:
    Maciej Kozak, Michał Taube, Maciej Gielnik, Zuzanna Pietralik
    Conference:
    3rd BIO Congress 3rd Congress of Polish Biosciences BIO2018 Through interdisciplinary approach into new solutions and 51st Meeting of the Polish Biochemical Society (year: 2018, pages: 12), Publisher: Polish Biochemical Society and of the Committee of Biochemistry and Biophysics Polish Academy of Sciences, Acta Biochimica Polonica Suppl 2/2018
    Date:
    conference 18-21 września
    Status:
    Published

  6. Radiation damage of proteins caused by synchrotron radiation – a case study based on bioSAXS experiments

    Authors:
    Augustyn Moliński, Michał Taube, Zuzanna Pietralik, Maciej Gielnik and Maciej Kozak
    Conference:
    14th International School and Symposium on Synchrotron Radiation in Natural Science (ISSRNS'2019) (year: 2019, pages: 84), Publisher: Polskie Towarzystwo Promieniowania Synchrotronowego
    Date:
    conference 9-14 czerwca
    Status:
    Published

  7. Small angle X-ray scattering studies of the full length human prion protein in different pH conditions

    Authors:
    Maciej Gielnik, Michał Taube, Igor Zhukov, Maciej Kozak
    Conference:
    12 Krajowe Sympozjum Użytkowników Promieniowania Synchrotronowego (year: 2017, pages: 37), Publisher: Polish Synchrotron Radiation Society, Synchrotron Radiation in Natural Science Vol. 16, No. 1-2 (2017)
    Date:
    conference 4-7 września
    Status:
    Published

  8. Phosphorylated prion peptide PrP40-49 unfolds WW domain of PIN1 peptidyl-prolyl cis-trans isomerase

    Authors:
    Maciej Gielnik, Igor Zhukov, Damian Neubauer, Wojciech Kamysz, Peter Bayer, Maciej Kozak
    Conference:
    12 Krajowe Sympozjum Użytkowników Promieniowania Synchrotronowego (year: 2017, pages: 53), Publisher: Polish Synchrotron Radiation Society, Synchrotron Radiation in Natural Science Vol. 16, No. 1-2 (2017)
    Date:
    conference 4-7 września
    Status:
    Published

  9. Low-resolution structure in solution of human prion protein PrP and the peptide designed on the N-terminal fragment of PrP protein.

    Authors:
    Maciej Kozak, Maciej Gielnik, Michał Taube, Igor Zhukov
    Conference:
    24th Polish Peptide Symposium (year: 2017, pages: 22), Publisher: Zakład Chemii Biomedycznej Uniwersytetu Gdańskiego
    Date:
    conference 3-7 września
    Status:
    Published

  10. Fibrillation of N-Terminal Prion Protein Fragment in Presence of Zinc Ions

    Authors:
    Maciej B. Gielnik, Michał Nowakowski, Aneta Szymańska, Igor Zhukov, Wojciech Maria Kwiatek, Maciej Leszek Kozak
    Conference:
    62nd Annual Meeting of the Biophysical Society, San Francisco (year: 2018, pages: 429a), Publisher: Elsevier, Biophysical Journal Biophysical Journal Volume 114, Issue 3, Supplement 1
    Date:
    conference 17-21 lutego
    Status:
    Published

  11. Preliminary results of human PrPC protein examination by spectroscopic techniques

    Authors:
    M. Nowakowski, J. Czapla-Masztafiak, M. Kozak, I.Zhukov and W. M. Kwiatek
    Conference:
    13th International School and Symposium on Synchrotron Radiation in Natural Science (year: 2016, pages: 90), Publisher: Polish Synchrotron Radiation Society, Synchrotron Radiation in Natural Science Vol. 15, No. 1-2 (2016)
    Date:
    conference 13-18 June 2016, Ustroń, Poland
    Status:
    Published
    DOI:
    link to the publication

  12. Wpływ surfaktantów dikationowych na strukturę drugorzędową wybranych peptydów: N-terminalnego fragmentu ludzkiego białka prionowego i peptydu Ac-PEPTIDES- NH 2

    Authors:
    Julia Ludwiczak, Igor Zhukov, Sylwia Rodziewicz Motowidło, Maciej Kozak
    Conference:
    Konferencja Polskiego Towarzystwa Fizyki Medycznej (year: 2017, pages: 47-48), Publisher: WCO,
    Date:
    conference 1-3 czerwca
    Status:
    Published

  13. Badania SAXS oraz dynamika konformacyjna amyloidogennej, nieustrukturyzowanej N-terminalnej domeny ludzkiego białka prionowego

    Authors:
    Maciej Gielnik, Igor Zhukov, Wojciech Kwiatek, Maciej Kozak
    Conference:
    Konfrencja Polskiego Towarzystwa Fizyki Medycznej (year: 2017, pages: 44-45), Publisher: WCO
    Date:
    conference 1-3 czerwca
    Status:
    Published

  14. Synchrotron - unikalne narzędzie w rękach biofizyka

    Authors:
    Maciej Kozak, Zuzanna Pietralik, Michał Taube, Maciej Gielnik, Witold Gospodarczyk, Żaneta Kołodziejska
    Conference:
    V Polska Konferencja Optyczna (year: 2017, pages: 6), Publisher: Sekcja Optyki Polskiego Towarzystwa Fizycznego
    Date:
    conference 2-6 lipca
    Status:
    Published

  15. Conformational Dynamics of Human Prion Protein and Binding Sites of Zn Cations

    Authors:
    Maciej Gielnik, Michał Nowakowski, Michał Taube, Igor Zhukov, Wojciech M. Kwiatek, Dmitry M. Lesovoy, Maciej Kozak
    Conference:
    62nd Annual Meeting of the Biophysical Society, San Francisco (year: 2018, pages: 387a), Publisher: Elsevier, Biophysical Journal Biophysical Journal Volume 114, Issue 3, Supplement 1
    Date:
    conference 17-21 lutego
    Status:
    Published

  16. Human prion protein at physiological pH: structural plasticity and dynamic processes

    Authors:
    Igor Zhukov, Maciej Gielnik, Lilia Zhukova, Dmitry M. Lesovoy, Maciej Kozak
    Conference:
    EUROMAR Warsaw 2017 - European Conference on Magnetic Resonance (year: 2017, pages: 76), Publisher: EUROMAR
    Date:
    conference 2-6 lipca
    Status:
    Published

  17. BioSAXS – a tool for structural studies of complex or disordered biomacromolecules

    Authors:
    Maciej Kozak, Michał Taube, Zuzanna Pietralik, Maciej Gielnik, Julia Ludwiczak, Aneta Szymańska, Igor Zhukov
    Conference:
    BIO 2016 Congress - 2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics (year: 2016, pages: 100), Publisher: Polish Biochemical Society and of the Committee of Biochemistry and Biophysics Polish Academy of Sciences, Acta Biochimica Polonica Suppl 2/2016
    Date:
    conference September 13th–16th
    Status:
    Published
    DOI:
    link to the publication

  18. Preliminary spectroscopic and thermodynamic studies of binding Zn2+ ions to human PrPC M.

    Authors:
    M. Gielnik, I. Zhukov and M. Kozak
    Conference:
    13th International School and Symposium on Synchrotron Radiation in Natural Science (year: 2016, pages: 103), Publisher: Polish Synchrotron Radiation Society, Synchrotron Radiation in Natural Science Vol. 15, No. 1-2 (2016)
    Date:
    conference 13-18 June 2016, Ustroń, Poland
    Status:
    Published
    DOI:
    link to the publication

  19. Dynamic Aspects of N- and C- Domain Interactions in Human Prion Protein PrPC (23-231) under Zn(II) Binding by Means of 15N Relaxation Data and Diffusion Measurements

    Authors:
    Igor Zhukov, Kosma Szutkowski, Lilia Zhukova, Wojciech Bal, Maciej Kozak
    Conference:
    XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS) (year: 2016, pages: post294), Publisher: ICMRBS
    Date:
    conference 21-26 sierpnia 2016
    Status:
    Published

  20. Coordination of Cu(II) in complex with human PrPC studied by XAS and AFM techniques

    Authors:
    Michał Nowakowski, Joanna Czapla- Masztafiak, Maciej Kozak, Igor Zhukov, Lilianna Zhukova, Jakub Szlachetko, Wojciech M. Kwiatek
    Conference:
    52nd Zakopane School of Physics (year: 2017, pages: 21-22), Publisher: IFJ PAN
    Date:
    conference 22-27 maja 2017
    Status:
    Published

  21. Spectroscopic and SAXS Studies of Human Prion Protein Variants Complexed with Divalent Cations

    Authors:
    Maciej Kozak Maciej Gielnik Michał Taube Igor Zhukov
    Conference:
    61st Annual Meeting of the Biophysical Society, New Orleans (USA) (year: 2017, pages: Biophysical Journal 112 (3), Supplement 1, p190a), Publisher: Elsevier Inc; Biophysical Journal 112 (3), Supplement 1,
    Date:
    conference 11-15 lutego
    Status:
    Published
    DOI:
    link to the publication

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Graphic design and implementation: Jakub Piotrowski