Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Impact of metallothionein polymorphism and metamorphism on cellular zinc homeostasis - the role of sequential, structural and thermodynamic diversity

2018/31/B/NZ1/00567

Keywords:

metallothionein

Descriptors:

  • NZ1_2: Biochemistry
  • NZ1_3: Biophysics
  • ST4_3: Molecular architecture and structure

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Wrocławski, Wydział Biotechnologii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Artur Krężel 

Number of co-investigators in the project: 8

Call: OPUS 16 - announced on 2018-09-14

Amount awarded: 2 200 400 PLN

Project start date (Y-m-d): 2019-07-24

Project end date (Y-m-d): 2023-03-23

Project duration:: 44 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. chromatograf FPLC.
  2. pH-metr z elektrodą.
  3. łaźnia wodna.
  4. dysku zewnętrznego SSD.
  5. oprogramowanie ProteinScape i Mascot.
  6. filtry optyczne.
  7. Zestaw komputerowy typu laptop z drukarką (7 000 PLN)
  8. waga analityczna.
  9. Spektrometr mas (498 000 PLN)

Information in the final report

  • Publication in academic press/journals (13)
  • Articles in post-conference publications (17)
  1. Metal- and Affinity-Specific Dual Labeling of Cysteine-Rich Proteins for Identification of Metal-Binding Sites
    Authors:
    Manuel David Peris-Díaz, Roman Guran, Ondrej Zitka, Vojtech Adam, and Artur Krężel
    Academic press:
    Anal. Chem (rok: 2020, tom: 92, strony: 12950−12958), Wydawca: ACS Publications
    Status:
    Published
    DOI:
    10.1021/acs.analchem.0c01604 - link to the publication
  2. Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn
    Authors:
    Karolina Szaro, Kinga Jurczak, Artur Krężel
    Academic press:
    Metallomics (rok: 2023, ), Wydawca: Oxford University Press
    Status:
    Submitted
    DOI:
    brak - link to the publication
  3. An Integrated Mass Spectrometry and Molecular Dynamics Simulations Approach Reveals the Spatial Organization Impact of Metal-Binding Sites on the Stability of Metal-Depleted Metallothionein‑2 Species
    Authors:
    Manuel David Peris-Díaz, Roman Guran, Carmen Domene, Vivian de los Rios, Ondrej Zitka, Vojtech Adam, Artur Krężel
    Academic press:
    Journal of the American Chemical Society (rok: 2021, tom: 143, strony: 16486–16501), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/jacs.1c05495 - link to the publication
  4. From methodological limitations to the function of metallothioneins – A guide to approaches for determining weak, moderate, and tight affinity zinc sites
    Authors:
    Adam Pomorski, Agnieszka Drozd, Anna Kocyła, Artur Krężel
    Academic press:
    Metallomics (rok: 2023, tom: 15, strony: mfad027), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/mtomcs/mfad027 - link to the publication
  5. Non-Conserved Amino Acid Residues Modulate the Thermodynamics of Zn(II) Binding to Classical ββα Zinc Finger Domains
    Authors:
    Katarzyna Kluska, Aleksandra Chorążewska, Manuel D. Peris-Diaz, Justyna Adamczyk, Artur Krężel
    Academic press:
    International Journal of Molecular Sciences (rok: 2022, tom: 23, strony: 14602), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms232314602 - link to the publication
  6. Phytochelatins as a dynamic system for Cd(II) buffering from the micro- to femtomolar range
    Authors:
    Joanna Wątły, Marek Łuczkowski, Michał Padjasek, Artur Krężel
    Academic press:
    Inorganic Chemistry (rok: 2021, tom: 60, strony: 4657-4675), Wydawca: American Chemical Society (ACS)
    Status:
    Published
    DOI:
    10.1021/acs.inorgchem.0c03639 - link to the publication
  7. OaAEP1 Ligase-Assisted Chemoenzymatic Synthesis of Full Cysteine-Rich Metal-Binding Cyanobacterial Metallothionein SmtA
    Authors:
    Anastasiia Antonenko, Avinash Kumar Singh, Karolina Mosna, Artur Krężel
    Academic press:
    Bioconjugate Chemistry (rok: 2023, tom: 34, strony: 719–727), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.bioconjchem.3c00037 - link to the publication
  8. The connection of α- and β-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties
    Authors:
    Avinash Kumar Singh, Adam Pomorski, Sylwia Wu, Manuel D. Peris-Diaz, Hanna Czepczyńska-Krężel, Artur Krężel
    Academic press:
    Metallomics (rok: 2023, tom: 15, strony: mfad029), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/mtomcs/mfad029 - link to the publication
  9. A chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 states
    Authors:
    Manuel David Peris-Díaz, Lukas Richtera, Ondrej Zitka, Artur Krężel, Vojtech Adam
    Academic press:
    Bioelectrochemistry (rok: 2020, tom: 134, strony: 107501), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bioelechem.2020.107501 - link to the publication
  10. Drug Conjugation via Maleimide–Thiol Chemistry Does Not Affect Targeting Properties of Cysteine-Containing Anti-FGFR1 Peptibodies
    Authors:
    Karolina Jendryczko, Jakub Rzeszotko, Mateusz A. Krzyścik, Anna Kocyła, Jakub Szymczyk, Jacek Otlewski, Anna Szlachcic
    Academic press:
    Molecular Pharmaceutics (rok: 2022, tom: 19, strony: 1422-1433), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.molpharmaceut.1c00946 - link to the publication
  11. Ion mobility mass spectrometry and molecular dynamics simulations unravel the conformational stability of zinc metallothionein-2 species
    Authors:
    Manuel D. Peris-Diaz, Alexey Barkhanskiy, Ellen Liggett, Perdita Barran, Artur Krężel
    Academic press:
    Chemical Communication (Camb.) (rok: 2023, tom: 59, strony: 4471-4474), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/d2cc06559b - link to the publication
  12. The Bioinorganic Chemistry of Mammalian Metallothioneins
    Authors:
    Artur Krężel, Wolfgang Maret
    Academic press:
    Chemical Reviews (rok: 2021, tom: 121, strony: 14594–14648), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.chemrev.1c00371 - link to the publication
  13. The Glutathione/Metallothionein System Challenges the Design of Efficient O2-Activating Copper Complexes
    Authors:
    Alice Santoro, Jenifer S. Calvo, Manuel David Peris-Diaz, Artur Krężel, Gabriele Meloni, Peter Faller
    Academic press:
    Angewandte Chemie-International Edition (rok: 2020, tom: 59, strony: 7830-7835), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/anie.201916316 - link to the publication
  1. Highly efficient sortase-A and Ulp1-mediated purification method for the expression and purification of recombinant human MT3 in bacterial system
    Authors:
    Avinash Kumar Singh, Artur Krężel
    Conference:
    EMBO (European Molecular Biology Organisation) Workshop: Chemical Biology 2022 (rok: 2022, ), Wydawca: EMBL, European Molecular Biology Laboratory
    Data:
    konferencja 05-08.
    Status:
    Published
  2. Metal-coupled folding as the driving force for zinc proteins function – insight into sequence-structure-stability relationship om zinc finger, hook and clasp domains
    Authors:
    Olga Kerber, Michał Padjasek, Józef Tran, Katarzyna Kluska, Anna Kocyła, Artur Krężel
    Conference:
    17th Naples Workshop on Bioactive Peptides (rok: 2022, ), Wydawca: The European Peptide Society
    Data:
    konferencja 16-18.06.
    Status:
    Published
  3. Native chemical ligation as a way for obtaining fully synthetic metallothioneins
    Authors:
    Józef Tran, Artur Krężel
    Conference:
    17th Naples Workshop on Bioactive Peptides (rok: 2022, ), Wydawca: The European Peptide Society
    Data:
    konferencja 16-18.06.
    Status:
    Published
  4. Regulacja i deregulacja funkcji białek cynkowych – walka dobrego z dobrym i złym
    Authors:
    Artur Krężel
    Conference:
    64. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2022, ), Wydawca: Uniwersytet Marii Curie-Skłodowskiej
    Data:
    konferencja 11-16.09.
    Status:
    Published
  5. Składanie białek z wykorzystaniem peptydowych motywów dimeryzujących zależnych od jonów Zn(II)
    Authors:
    Anna Kocyła, Artur Krężel
    Conference:
    62. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2 – 6 września
    Status:
    Published
  6. Zn(II) binding properties of human metallothioneins – Investigation of three classes of Zn(II) affinities
    Authors:
    Sylwia Siadul, Karolina Szaro, Józef Ba Tran, Agnieszka Drozd, Artur Krężel
    Conference:
    The Protein Society - 35th Anniversary Symposium (rok: 2021, ), Wydawca: The Protein Society
    Data:
    konferencja 7-14 lipca
    Status:
    Published
  7. Relation between fluctuations of free ZN(II) concentrations and formation of Zn(II)-binding sites in cysteine-rich motif of MTF-1
    Authors:
    Katarzyna Kluska, Karolina Szaro, Artur Krężel
    Conference:
    17th Naples Workshop on Bioactive Peptides (rok: 2022, ), Wydawca: The European Peptide Society
    Data:
    konferencja 16-18.06.
    Status:
    Published
  8. Zinc to silver swap in zinc finger domains disrupts their native structures and DNA recognition
    Authors:
    Artur Krężel
    Conference:
    International Conference of Trace Elements and Minerals (rok: 2022, ), Wydawca: RWTH Aachen University
    Data:
    konferencja 5-10.06
    Status:
    Published
  9. Differences in Zn2+ binding properties of human metallothioneins natural variants
    Authors:
    Sylwia Wu, Adam Pomorski, Artur Krężel
    Conference:
    16th European Biological Inorganic Chemistry Conference (rok: 2022, ), Wydawca: University Grenoble Alpes
    Data:
    konferencja 17-21.07.
    Status:
    Published
  10. Modification of zinc-sulfur clusters by allicin leads to Zn(II) dissociation from human metallothionein-2
    Authors:
    Karolina Szaro, Adam Pomorski, Sylwia Wu, Olga Kerber, Artur Krężel
    Conference:
    International Conference of Trace Elements and Minerals 2022 (ICTEM) (rok: 2022, ), Wydawca: RWTH Aachen University
    Data:
    konferencja 5-10.06
    Status:
    Published
  11. Wpływ sposobu koordynacji oraz powinowactwa jonów Zn(II) na komórkową funkcję metalobiałek
    Authors:
    Katarzyna Kluska, Anna Kocyła, Olga Kerber, Józef Tran, Michał Padjasek, Manuel Peris-Díaz, Artur Krężel
    Conference:
    62. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2-6.09.
    Status:
    Published
  12. Zn(II)-binding properties of metallothioneins fromvarious domains of life
    Authors:
    Karolina Szaro, Sylwia Siadul, Kinga Jurczak, Artur Krężel
    Conference:
    The Protein Society - 35th Anniversary Symposium (rok: 2021, ), Wydawca: The Protein Society
    Data:
    konferencja 7-14.07
    Status:
    Published
  13. Human metallothionein isoforms differ in their Zn(II) binding properties - Stability and reactivity studies
    Authors:
    Sylwia Wu, Karolina Szaro, Agnieszka Drozd, Artur Krężel
    Conference:
    International Conference of Trace Elements and Minerals - ICTEM 2022 (rok: 2022, ), Wydawca: RWTH Aachen University
    Data:
    konferencja 5-10.06.
    Status:
    Published
  14. Plant metallothioneins bind Zn(II) with different affinities
    Authors:
    Karolina. Szaro, Kinga Jurczak, Artur Krężel
    Conference:
    16th European Biological Inorganic Chemistry Conference (rok: 2022, ), Wydawca: University Grenoble Alpes
    Data:
    konferencja 17-21.07.
    Status:
    Published
  15. Irreversible silver binding to zinc finger peptides causes the formation of multinuclear AgnSn clusters and loss of their function
    Authors:
    Artur Krężel
    Conference:
    International Conference on Metal-Binding Peptides (rok: 2022, ), Wydawca: MBP Nancy
    Data:
    konferencja 5-8.07
    Status:
    Published
  16. Relationships of the structural and zinc buffering properties of human metallothioneins
    Authors:
    Manuel D. Peris-Diaz, Agnieszka Drozd, Dominka Wojewska, Roman Guran, Adam Pomorski, Ondrej Zitka, Vojtech Adam, Artur Krężel
    Conference:
    The 6th Meeting of International Society for Zinc Biology (rok: 2019, ), Wydawca: International Society for Zinc Biology
    Data:
    konferencja 9-13.09.
    Status:
    Published
  17. Silver binding to a peptide model of Rad50 interprotein hook domain
    Authors:
    Olga Kerber, Józef Tran, Alicja Misiaszek, Aleksandra Chorążewska, Artur Krężel
    Conference:
    International Conference on Metal-Binding Peptides (rok: 2022, ), Wydawca: MBP Nancy
    Data:
    konferencja 5-8.07.
    Status:
    Published

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