Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

Impact of metallothionein polymorphism and metamorphism on cellular zinc homeostasis - the role of sequential, structural and thermodynamic diversity

2018/31/B/NZ1/00567

Keywords:

metallothionein

Descriptors:

  • NZ1_2: Biochemistry
  • NZ1_3: Biophysics
  • ST4_3: Molecular architecture and structure

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Wrocławski, Wydział Biotechnologii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Artur Krężel 

Number of co-investigators in the project: 8

Call: OPUS 16 - announced on 2018-09-14

Amount awarded: 2 200 400 PLN

Project start date (Y-m-d): 2019-07-24

Project end date (Y-m-d): 2023-03-23

Project duration:: 44 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Zestaw komputerowy typu laptop z drukarką (7 000 PLN)
  2. chromatograf FPLC.
  3. pH-metr z elektrodą.
  4. waga analityczna.
  5. filtry optyczne.
  6. łaźnia wodna.
  7. dysku zewnętrznego SSD.
  8. oprogramowanie ProteinScape i Mascot.
  9. Spektrometr mas (498 000 PLN)

Information in the final report

  • Publication in academic press/journals (13)
  • Articles in post-conference publications (17)
  1. Ion mobility mass spectrometry and molecular dynamics simulations unravel the conformational stability of zinc metallothionein-2 species
    Authors:
    Manuel D. Peris-Diaz, Alexey Barkhanskiy, Ellen Liggett, Perdita Barran, Artur Krężel
    Academic press:
    Chemical Communication (Camb.) (rok: 2023, tom: 59, strony: 4471-4474), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/d2cc06559b - link to the publication
  2. Non-Conserved Amino Acid Residues Modulate the Thermodynamics of Zn(II) Binding to Classical ββα Zinc Finger Domains
    Authors:
    Katarzyna Kluska, Aleksandra Chorążewska, Manuel D. Peris-Diaz, Justyna Adamczyk, Artur Krężel
    Academic press:
    International Journal of Molecular Sciences (rok: 2022, tom: 23, strony: 14602), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms232314602 - link to the publication
  3. The Bioinorganic Chemistry of Mammalian Metallothioneins
    Authors:
    Artur Krężel, Wolfgang Maret
    Academic press:
    Chemical Reviews (rok: 2021, tom: 121, strony: 14594–14648), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.chemrev.1c00371 - link to the publication
  4. An Integrated Mass Spectrometry and Molecular Dynamics Simulations Approach Reveals the Spatial Organization Impact of Metal-Binding Sites on the Stability of Metal-Depleted Metallothionein‑2 Species
    Authors:
    Manuel David Peris-Díaz, Roman Guran, Carmen Domene, Vivian de los Rios, Ondrej Zitka, Vojtech Adam, Artur Krężel
    Academic press:
    Journal of the American Chemical Society (rok: 2021, tom: 143, strony: 16486–16501), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/jacs.1c05495 - link to the publication
  5. Metal- and Affinity-Specific Dual Labeling of Cysteine-Rich Proteins for Identification of Metal-Binding Sites
    Authors:
    Manuel David Peris-Díaz, Roman Guran, Ondrej Zitka, Vojtech Adam, and Artur Krężel
    Academic press:
    Anal. Chem (rok: 2020, tom: 92, strony: 12950−12958), Wydawca: ACS Publications
    Status:
    Published
    DOI:
    10.1021/acs.analchem.0c01604 - link to the publication
  6. Phytochelatins as a dynamic system for Cd(II) buffering from the micro- to femtomolar range
    Authors:
    Joanna Wątły, Marek Łuczkowski, Michał Padjasek, Artur Krężel
    Academic press:
    Inorganic Chemistry (rok: 2021, tom: 60, strony: 4657-4675), Wydawca: American Chemical Society (ACS)
    Status:
    Published
    DOI:
    10.1021/acs.inorgchem.0c03639 - link to the publication
  7. A chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 states
    Authors:
    Manuel David Peris-Díaz, Lukas Richtera, Ondrej Zitka, Artur Krężel, Vojtech Adam
    Academic press:
    Bioelectrochemistry (rok: 2020, tom: 134, strony: 107501), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bioelechem.2020.107501 - link to the publication
  8. From methodological limitations to the function of metallothioneins – A guide to approaches for determining weak, moderate, and tight affinity zinc sites
    Authors:
    Adam Pomorski, Agnieszka Drozd, Anna Kocyła, Artur Krężel
    Academic press:
    Metallomics (rok: 2023, tom: 15, strony: mfad027), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/mtomcs/mfad027 - link to the publication
  9. OaAEP1 Ligase-Assisted Chemoenzymatic Synthesis of Full Cysteine-Rich Metal-Binding Cyanobacterial Metallothionein SmtA
    Authors:
    Anastasiia Antonenko, Avinash Kumar Singh, Karolina Mosna, Artur Krężel
    Academic press:
    Bioconjugate Chemistry (rok: 2023, tom: 34, strony: 719–727), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.bioconjchem.3c00037 - link to the publication
  10. The Glutathione/Metallothionein System Challenges the Design of Efficient O2-Activating Copper Complexes
    Authors:
    Alice Santoro, Jenifer S. Calvo, Manuel David Peris-Diaz, Artur Krężel, Gabriele Meloni, Peter Faller
    Academic press:
    Angewandte Chemie-International Edition (rok: 2020, tom: 59, strony: 7830-7835), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/anie.201916316 - link to the publication
  11. The connection of α- and β-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties
    Authors:
    Avinash Kumar Singh, Adam Pomorski, Sylwia Wu, Manuel D. Peris-Diaz, Hanna Czepczyńska-Krężel, Artur Krężel
    Academic press:
    Metallomics (rok: 2023, tom: 15, strony: mfad029), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/mtomcs/mfad029 - link to the publication
  12. Drug Conjugation via Maleimide–Thiol Chemistry Does Not Affect Targeting Properties of Cysteine-Containing Anti-FGFR1 Peptibodies
    Authors:
    Karolina Jendryczko, Jakub Rzeszotko, Mateusz A. Krzyścik, Anna Kocyła, Jakub Szymczyk, Jacek Otlewski, Anna Szlachcic
    Academic press:
    Molecular Pharmaceutics (rok: 2022, tom: 19, strony: 1422-1433), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.molpharmaceut.1c00946 - link to the publication
  13. Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn
    Authors:
    Karolina Szaro, Kinga Jurczak, Artur Krężel
    Academic press:
    Metallomics (rok: 2023, ), Wydawca: Oxford University Press
    Status:
    Submitted
    DOI:
    brak - link to the publication
  1. Differences in Zn2+ binding properties of human metallothioneins natural variants
    Authors:
    Sylwia Wu, Adam Pomorski, Artur Krężel
    Conference:
    16th European Biological Inorganic Chemistry Conference (rok: 2022, ), Wydawca: University Grenoble Alpes
    Data:
    konferencja 17-21.07.
    Status:
    Published
  2. Metal-coupled folding as the driving force for zinc proteins function – insight into sequence-structure-stability relationship om zinc finger, hook and clasp domains
    Authors:
    Olga Kerber, Michał Padjasek, Józef Tran, Katarzyna Kluska, Anna Kocyła, Artur Krężel
    Conference:
    17th Naples Workshop on Bioactive Peptides (rok: 2022, ), Wydawca: The European Peptide Society
    Data:
    konferencja 16-18.06.
    Status:
    Published
  3. Modification of zinc-sulfur clusters by allicin leads to Zn(II) dissociation from human metallothionein-2
    Authors:
    Karolina Szaro, Adam Pomorski, Sylwia Wu, Olga Kerber, Artur Krężel
    Conference:
    International Conference of Trace Elements and Minerals 2022 (ICTEM) (rok: 2022, ), Wydawca: RWTH Aachen University
    Data:
    konferencja 5-10.06
    Status:
    Published
  4. Relation between fluctuations of free ZN(II) concentrations and formation of Zn(II)-binding sites in cysteine-rich motif of MTF-1
    Authors:
    Katarzyna Kluska, Karolina Szaro, Artur Krężel
    Conference:
    17th Naples Workshop on Bioactive Peptides (rok: 2022, ), Wydawca: The European Peptide Society
    Data:
    konferencja 16-18.06.
    Status:
    Published
  5. Zn(II) binding properties of human metallothioneins – Investigation of three classes of Zn(II) affinities
    Authors:
    Sylwia Siadul, Karolina Szaro, Józef Ba Tran, Agnieszka Drozd, Artur Krężel
    Conference:
    The Protein Society - 35th Anniversary Symposium (rok: 2021, ), Wydawca: The Protein Society
    Data:
    konferencja 7-14 lipca
    Status:
    Published
  6. Native chemical ligation as a way for obtaining fully synthetic metallothioneins
    Authors:
    Józef Tran, Artur Krężel
    Conference:
    17th Naples Workshop on Bioactive Peptides (rok: 2022, ), Wydawca: The European Peptide Society
    Data:
    konferencja 16-18.06.
    Status:
    Published
  7. Regulacja i deregulacja funkcji białek cynkowych – walka dobrego z dobrym i złym
    Authors:
    Artur Krężel
    Conference:
    64. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2022, ), Wydawca: Uniwersytet Marii Curie-Skłodowskiej
    Data:
    konferencja 11-16.09.
    Status:
    Published
  8. Zinc to silver swap in zinc finger domains disrupts their native structures and DNA recognition
    Authors:
    Artur Krężel
    Conference:
    International Conference of Trace Elements and Minerals (rok: 2022, ), Wydawca: RWTH Aachen University
    Data:
    konferencja 5-10.06
    Status:
    Published
  9. Human metallothionein isoforms differ in their Zn(II) binding properties - Stability and reactivity studies
    Authors:
    Sylwia Wu, Karolina Szaro, Agnieszka Drozd, Artur Krężel
    Conference:
    International Conference of Trace Elements and Minerals - ICTEM 2022 (rok: 2022, ), Wydawca: RWTH Aachen University
    Data:
    konferencja 5-10.06.
    Status:
    Published
  10. Plant metallothioneins bind Zn(II) with different affinities
    Authors:
    Karolina. Szaro, Kinga Jurczak, Artur Krężel
    Conference:
    16th European Biological Inorganic Chemistry Conference (rok: 2022, ), Wydawca: University Grenoble Alpes
    Data:
    konferencja 17-21.07.
    Status:
    Published
  11. Relationships of the structural and zinc buffering properties of human metallothioneins
    Authors:
    Manuel D. Peris-Diaz, Agnieszka Drozd, Dominka Wojewska, Roman Guran, Adam Pomorski, Ondrej Zitka, Vojtech Adam, Artur Krężel
    Conference:
    The 6th Meeting of International Society for Zinc Biology (rok: 2019, ), Wydawca: International Society for Zinc Biology
    Data:
    konferencja 9-13.09.
    Status:
    Published
  12. Silver binding to a peptide model of Rad50 interprotein hook domain
    Authors:
    Olga Kerber, Józef Tran, Alicja Misiaszek, Aleksandra Chorążewska, Artur Krężel
    Conference:
    International Conference on Metal-Binding Peptides (rok: 2022, ), Wydawca: MBP Nancy
    Data:
    konferencja 5-8.07.
    Status:
    Published
  13. Składanie białek z wykorzystaniem peptydowych motywów dimeryzujących zależnych od jonów Zn(II)
    Authors:
    Anna Kocyła, Artur Krężel
    Conference:
    62. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2 – 6 września
    Status:
    Published
  14. Wpływ sposobu koordynacji oraz powinowactwa jonów Zn(II) na komórkową funkcję metalobiałek
    Authors:
    Katarzyna Kluska, Anna Kocyła, Olga Kerber, Józef Tran, Michał Padjasek, Manuel Peris-Díaz, Artur Krężel
    Conference:
    62. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2-6.09.
    Status:
    Published
  15. Highly efficient sortase-A and Ulp1-mediated purification method for the expression and purification of recombinant human MT3 in bacterial system
    Authors:
    Avinash Kumar Singh, Artur Krężel
    Conference:
    EMBO (European Molecular Biology Organisation) Workshop: Chemical Biology 2022 (rok: 2022, ), Wydawca: EMBL, European Molecular Biology Laboratory
    Data:
    konferencja 05-08.
    Status:
    Published
  16. Irreversible silver binding to zinc finger peptides causes the formation of multinuclear AgnSn clusters and loss of their function
    Authors:
    Artur Krężel
    Conference:
    International Conference on Metal-Binding Peptides (rok: 2022, ), Wydawca: MBP Nancy
    Data:
    konferencja 5-8.07
    Status:
    Published
  17. Zn(II)-binding properties of metallothioneins fromvarious domains of life
    Authors:
    Karolina Szaro, Sylwia Siadul, Kinga Jurczak, Artur Krężel
    Conference:
    The Protein Society - 35th Anniversary Symposium (rok: 2021, ), Wydawca: The Protein Society
    Data:
    konferencja 7-14.07
    Status:
    Published

O Narodowym Centrum Nauki

Finansowanie nauki

Współpraca zagraniczna

Centrum prasowe

Deklaracja dostępności
Przetwarzanie danych osobowych
Polityka cookies

Partnerzy