Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Cellular proteases during coronaviral infection

2017/27/B/NZ6/02488

Keywords:

coronavirus protease TMPRSS2 cathepsin fusion

Descriptors:

  • NZ6_5: Virology
  • NZ3_1: Cell biology
  • NZ1_8: Tissue engineering

Panel:

NZ6 - Human and animal immunology and infection: immunity, immune disorders, immunotherapy, infectious and invasive diseases, microbiology, transplantology, allergology

Host institution :

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Krzysztof Pyrć 

Number of co-investigators in the project: 3

Call: OPUS 14 - announced on 2017-09-15

Amount awarded: 1 969 600 PLN

Project start date (Y-m-d): 2018-07-31

Project end date (Y-m-d): 2024-12-30

Project duration:: 36 months (the same as in the proposal)

Project status: Pending project

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Information in the final report

  • Publication in academic press/journals (23)
  1. Ceragenins exhibit antiviral activity against SARS-CoV-2 by increasing the expression and release of type I interferons upon activation of the host's immune response
    Authors:
    Łukasz Suprewicz, Artur Szczepański, Marzena Lenart, Ewelina Piktel, Krzysztof Fiedoruk, Emilia Barreto-Duran, Anna Kula-Pacurar, Paul B. Savage, Aleksandra Milewska, Robert Bucki, Krzysztof Pyrć
    Academic press:
    Antiviral Research (rok: 2023, tom: 217, strony: 105676), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.antiviral.2023.105676 - link to the publication
  2. SARS-CoV-2 infection impairs NK cell functions via activation of the LLT1-CD161 axis
    Authors:
    Marzena Lenart, Magdalena Gorecka, Michal Bochenek, Emilia Barreto-Duran, Artur Szczepanski, Adrianna Gałuszka-Bulaga, Natalia Mazur-Panasiuk, Kazimierz Weglarczyk, Andzelika Siwiec-Kozlik, Mariusz Korkosz, Paweł P. Łabaj, Monika Baj-Krzyworzeka, Maciej Siedlar, Krzysztof Pyrc
    Academic press:
    Frontiers in Immunology (rok: 2023, tom: 14, strony: 1123155), Wydawca: FRONTIERS MEDIA SA
    Status:
    Published
    DOI:
    10.3389/fimmu.2023.1123155 - link to the publication
  3. SARS-CoV-2 infects an in vitro model of the human developing pancreas through endocytosis
    Authors:
    Szlachcic WJ, Dabrowska A, Milewska A, Ziojla N, Blaszczyk K, Barreto-Duran E, Sanak M, Surmiak M, Owczarek K, Grzanka D, Durzynska J, Pyrc K, Borowiak M.
    Academic press:
    iScience (rok: 2022, tom: 25, strony: 104594), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.isci.2022.104594 - link to the publication
  4. The interplay between the airway epithelium and tissue macrophages during the SARS-CoV-2 infection
    Authors:
    Barreto-Duran E, Szczepański A, Gałuszka-Bulaga A, Surmiak M, Siedlar M, Sanak M, Rajfur Z, Milewska A, Lenart M, Pyrć K.
    Academic press:
    Frontiers in Immunology (rok: 2022, tom: 13, strony: 991991), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fimmu.2022.991991 - link to the publication
  5. Battle at the entrance gate: CIITA as a weapon to prevent the internalization of SARS-CoV-2 and Ebola viruses
    Authors:
    Butowt R, Pyrc K, von Bartheld CS.
    Academic press:
    Signal transduction and targeted therapy. (rok: 2020, tom: 5, strony: 278), Wydawca: NATURE PUBLISHING GROUP
    Status:
    Published
    DOI:
    10.1038/s41392-020-00405-2 - link to the publication
  6. Canine Respiratory Coronavirus, Bovine Coronavirus, and Human Coronavirus OC43: Receptors and Attachment Facto
    Authors:
    Artur Szczepanski, Katarzyna Owczarek, Monika Bzowska, Katarzyna Gula, Inga Drebot, Marek Ochman, Beata Maksym, Zenon Rajfur, Judy A Mitchell, Krzysztof Pyrc
    Academic press:
    Viruses (rok: 2019, tom: 11, strony: E328), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/v11040328 - link to the publication
  7. Identification of Cellular Factors Required for SARS- CoV-2 Replication.
    Authors:
    Synowiec A, Jedrysik M, Branicki W, Klajmon A, Lei J, Owczarek K, Suo C, Szczepanski A, Wang J, Zhang P, Labaj PP, Pyrc K.
    Academic press:
    Cells (rok: 2021, tom: 10(11), strony: 3159), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/cells10113159 - link to the publication
  8. Kallikrein 13 serves as a priming protease during infection by the human coronavirus HKU1
    Authors:
    Milewska A, Falkowski K, Kulczycka M, Bielecka E, Naskalska A, Mak P, Lesner A, Ochman M, Urlik M, Diamandis E, Prassas I, Potempa J, Kantyka T, Pyrc K.
    Academic press:
    Science Signaling (rok: 2020, tom: 13, strony: 659), Wydawca: AMER ASSOC ADVANCEMENT SCIENCE
    Status:
    Published
    DOI:
    10.1126/scisignal.aba9902 - link to the publication
  9. Replication of Severe Acute Respiratory Syndrome Coronavirus 2 in Human Respiratory Epithelium
    Authors:
    Aleksandra Milewska, Anna Kula-Pacurar, Jakub Wadas, Agnieszka Suder, Artur Szczepanski, Agnieszka Dabrowska, Katarzyna Owczarek, Alessandro Marcello, Marek Ochman, Tomasz Stacel, Zenon Rajfur, Marek Sanak, Pawel Labaj, Wojciech Branicki, Krzysztof Pyrc
    Academic press:
    Journal of Virology (rok: 2020, tom: 94, strony: e00957-20), Wydawca: AMER SOC MICROBIOLOGY
    Status:
    Published
    DOI:
    10.1128/JVI.00957-20 - link to the publication
  10. Seleno-Functionalization of Quercetin Improves the Non-Covalent Inhibition of Mpro and Its Antiviral Activity in Cells against SARS-CoV-2.
    Authors:
    Mangiavacchi F, Botwina P, Menichetti E, Bagnoli L, Rosati O, Marini F, Fonseca SF, Abenante L, Alves D, Dabrowska A, Kula-Pacurar A, Ortega-Alarcon D, Jimenez-Alesanco A, Ceballos-Laita L, Vega S, Rizzuti B, Abian O, Lenardão EJ, Velazquez- Campoy A, Pyrc K, Sancineto L, Santi C.
    Academic press:
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (rok: 2021, tom: 22(13), strony: 7048), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms22137048 - link to the publication
  11. The SARS-CoV-2 ORF10 is not essential in vitro or in vivo in humans
    Authors:
    Pancer K, Milewska A, Owczarek K, Dabrowska A, Kowalski M, Łabaj PP, Branicki W, Sanak M, Pyrc K.
    Academic press:
    Plos Pathogens (rok: 2020, tom: 16, strony: e1008959), Wydawca: PUBLIC LIBRARY SCIENCE
    Status:
    Published
    DOI:
    10.1371/journal.ppat.1008959 - link to the publication
  12. Vaccination versus SARS-CoV-2 Omicron: three vaccine doses win the battle
    Authors:
    Popowicz GM, Pyrc K, Hadian K.
    Academic press:
    Signal Transduction and Targeted Therapy (rok: 2022, tom: 7, strony: 140), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41392-022-01000-3 - link to the publication
  13. Zoonotic spill-over of SARS-CoV-2: mink-adapted virus in humans
    Authors:
    Lukasz Rabalski, Maciej Kosinski, Natalia Mazur-Panasiuk, Boguslaw Szewczyk, Krystyna Bienkowska-Szewczyk, Ravi Kant, Tarja Sironen, Krzysztof Pyrc, Maciej Grzybek
    Academic press:
    Clinical Microbiology and Infection (rok: 2022, tom: 28, strony: 451.e1-451.e4), Wydawca: European Society of Clinical Microbiology and Infectious Diseases / Elsevier
    Status:
    Published
    DOI:
    10.1016/j.cmi.2021.12.001 - link to the publication
  14. Berberine Hampers Influenza A Replication through Inhibition of MAPK/ERK Pathway
    Authors:
    Paweł Botwina, Katarzyna Owczarek, Zenon Rajfur, Marek Ochman, Maciej Urlik, Maria Nowakowska, Krzysztof Szczubiałka, Krzysztof Pyrc
    Academic press:
    Viruses-Basel (rok: 2020, tom: 12, strony: 344), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/v12030344 - link to the publication
  15. Functional Severe Acute Respiratory Syndrome Coronavirus 2 Virus-Like Particles From Insect Cells.
    Authors:
    Naskalska A, Dabrowska A, Szczepanski A, Jasik KP, Gromadzka B, Pyrc K.
    Academic press:
    Frontiers in Microbiology (rok: 2021, tom: 12, strony: 732998), Wydawca: FRONTIERS MEDIA SA
    Status:
    Published
    DOI:
    10.3389/fmicb.2021.732998 - link to the publication
  16. HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV
    Authors:
    Milewska A, Chi Y, Szczepanski A, Barreto-Duran E, Dabrowska A, Botwina P, Obloza M, Liu K, Liu D, Guo X, Ge Y, Li J, Cui L, Ochman M, Urlik M, Rodziewicz-Motowidlo S, Zhu F, Szczubialka K, Nowakowska M, Pyrc K.
    Academic press:
    Journal of Virology (rok: 2021, tom: 95, strony: e01622-20), Wydawca: AMER SOC MICROBIOLOGY
    Status:
    Published
    DOI:
    10.1128/JVI.01622-20 - link to the publication
  17. Acriflavine, a clinically approved drug, inhibits SARS-CoV-2 and other betacoronaviruses
    Authors:
    Napolitano V, Dabrowska A, Schorpp K, Mourão A, Barreto-Duran E, Benedyk M, Botwina P, Brandner S, Bostock M, Chykunova Y, Czarna A, Dubin G, Fröhlich T, Hölscher M, Jedrysik M, Matsuda A, Owczarek K, Pachota M, Plettenburg O, Potempa J, Rothenaigner I, Schlauderer F, Slysz K, Szczepanski A, Greve-Isdahl Mohn K, Blomberg B, Sattler M, Hadian K, Popowicz GM, Pyrc K.
    Academic press:
    Cell Chemical Biology (rok: 2022, tom: 29, strony: 774-784.e8), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.chembiol.2021.11.006 - link to the publication
  18. Ozone Treatment Is Insufficient to Inactivate SARS- CoV-2 Surrogate under Field Conditions.
    Authors:
    Mazur-Panasiuk N, Botwina P, Kutaj A, Woszczyna D, Pyrc K.
    Academic press:
    Antioxidants (Basel). (rok: 2021, tom: 10(9):1480., strony: 1480), Wydawca: MDPI
    Status:
    Published
    DOI:
    0.3390/antiox10091480 - link to the publication
  19. Refolding of lid subdomain of SARS-CoV-2 nsp14 upon nsp10 interaction releases exonuclease activity
    Authors:
    Czarna A, Plewka J, Kresik L, Matsuda A, Karim A, Robinson C, O'Byrne S, Cunningham F, Georgiou I, Wilk P, Pachota M, Popowicz G, Wyatt PG, Dubin G, Pyrć K.
    Academic press:
    Structure (rok: 2022, tom: 30, strony: 1050-1054.e2), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.str.2022.04.014 - link to the publication
  20. Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
    Authors:
    Kula-Pacurar A, Wadas J, Suder A, Szczepanski A, Milewska A, Ochman M, Stacel T, Pyrc K.
    Academic press:
    Journal of visualized experiments : JoVE. (rok: 2020, tom: 166, strony: -), Wydawca: JOURNAL OF VISUALIZED EXPERIMENTS
    Status:
    Published
    DOI:
    10.3791/62067 - link to the publication
  21. Zika virus: mapping and reprogramming the entry
    Authors:
    Katarzyna Owczarek, Yuliya Chykunova, Christian Jassoy, Beata Maksym, Zenon Rajfur, Krzysztof Pyrc
    Academic press:
    Cell Communication and Signaling (rok: 2019, tom: 17, strony: 41), Wydawca: BioMed Central
    Status:
    Published
    DOI:
    10.1186/s12964-019-0349-z - link to the publication
  22. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): a Systemic Infection
    Authors:
    Synowiec A, Szczepański A, Barreto-Duran E, Lie LK, Pyrc K.
    Academic press:
    Clinical Microbiology Reviews (rok: 2021, tom: 34, strony: e00133-20), Wydawca: AMER SOC MICROBIOLOGY
    Status:
    Published
    DOI:
    10.1128/CMR.00133-20 - link to the publication
  23. l-Arginine Improves Solubility and ANTI SARS- CoV-2 Mpro Activity of Rutin but Not the Antiviral Activity in Cells
    Authors:
    Sancineto L, Ostacolo C, Ortega-Alarcon D, Jimenez-Alesanco A, Ceballos-Laita L, Vega S, Abian O, Velazquez-Campoy A, Moretti S, Dabrowska A, Botwina P, Synowiec A, Kula- Pacurar A, Pyrc K, Iraci N, Santi C.
    Academic press:
    Molecules (rok: 2021, tom: 26(19), strony: 6062), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules26196062 - link to the publication

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