Global and local chromatin reorganisation in cellular response to DNA double-strand breaks, investigated with super-resolution fluorescence microscopy (dSTORM/SMLM) and fluorescence resonance energy transfer (FLIM/FRET)
2017/27/B/NZ3/01065
Keywords
- DNA
- DNA repair
- double-strand DNA breaks
- single-strand DNA breaks
- chromatin reorganisation
- superresolution microscopy
- single molecule localisation microscopy (SMLM)
- direct stochastic optical reconstruction microscopy (dSTORM)
- fluorescence lifetime microscopy (FLIM)
- Förster Resonance Energy Transfer (FRET)
- FLIM/FRET
Descriptors
- NZ3_1: Biologia komórki
- NZ1_3: Biofizyka
- NZ2_5: Cytogenetyka
Panel
NZ3 – Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology
Host institution
Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii
Voivodeship: małopolskie
Principal investigator (from the host institution)
Number of co-investigators in the project: 3
Call
OPUS 14 – announced on 2017-09-15
- Amount awarded
- 1 642 400 PLN
- Project start date
- 2018-07-26
- Project end date
- 2023-07-25
- Project duration
- 60 months (the same as in the proposal)
- Project status
- Project settled
Project description
Download the project description in a pdf file
Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.
Information in the final report
Publication in academic press/journals (2)
-
Translocation of chromatin proteins to nucleoli-The influence of protein dynamics on post-fixation localization
- Authors:
- Zarębski M, Bosire R, Wesołowska J, Szelest O, Eatmann A, Jasińska-Konior K, Kepp O, Kroemer G, Szabo G, Dobrucki JW.
- Academic press:
- Cytometry Part A (year: 2021, volume: 99(12), pages: 1230-1239), Publisher: Wiley
- Status:
- Published
- DOI:
- 10.1002/cyto.a.24464 – link to the publication
-
STRIDE-a fluorescence method for direct, specific in situ detection of individual single- or double-strand DNA breaks in fixed cells
- Authors:
- Kordon, MM; Zarebski, M; Solarczyk, K; Ma, H; Pederson, T; Dobrucki, J
- Academic press:
- Nucleic Acids Research (year: 2020, volume: 48(3), pages: e14), Publisher: Oxford Academic
- Status:
- Published
- DOI:
- 10.1093/nar/gkz1118 – link to the publication