Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

Gasdermin as a potential permeabilizing agent of outer cell membrane of Pseudomonas aeruginosa in order to enhanced bactericidal effect of recombinant phage endolysins

2017/27/B/NZ6/00199

Keywords:

endolysin pyroptosis gasdermin Pseudomonas aeruginosa

Descriptors:

  • NZ6_5: Virology
  • NZ6_4: Bacteriology
  • NZ6_1: Adaptive and innate immunity

Panel:

NZ6 - Human and animal immunology and infection: immunity, immune disorders, immunotherapy, infectious and invasive diseases, microbiology, transplantology, allergology

Host institution :

Uniwersytet Jana Kochanowskiego w Kielcach, Wydział Matematyczno-Przyrodniczy

woj. świętokrzyskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Michał Arabski 

Number of co-investigators in the project: 8

Call: OPUS 14 - announced on 2017-09-15

Amount awarded: 1 466 200 PLN

Project start date (Y-m-d): 2018-08-14

Project end date (Y-m-d): 2023-08-13

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. oprogramowania GeneScript GX.
  2. autoklaw (35 000 PLN)
  3. inkubator CO2 (13 000 PLN)
  4. wielofunkcyjny czytnik płytek titracyjnych Varioskan LUX (170 000 PLN)
  5. cytometr przepływowy Millipore Guava Easycyte (180 000 PLN)

Information in the final report

  • Publication in academic press/journals (8)
  1. PEGylation of dendronized silver nanoparticles increases the binding affinity of antimicrobial proteins
    Authors:
    Karol Ciepluch, Kinga Skrzyniarz, Justyna Zdańska, Andrea Barrios-Gumiel, Javier Sánchez-Nieves, F. Javier de la Mata, Barbara Maciejewska, Zuzanna Drulis-Kawa, Maria Bryszewska, Michał Arabski
    Academic press:
    Journal of Molecular Liquids (rok: 2020, tom: 319, strony: 45304), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.molliq.2020.114339 - link to the publication
  2. The influence of cationic dendrimers on antibacterial activity of phage endolysin against P.aeruginosa cells
    Authors:
    Karol Ciepluch, Barbara Maciejewska,Katarzyna Gałczyńska, Dorota Kuc-Ciepluch, Maria Bryszewska,Dietmar Appelhans, Zuzanna Drulis-Kawa, Michał Arabski
    Academic press:
    Bioorganic Chemistry (rok: 2019, tom: 91, strony: 45297), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bioorg.2019.103121 - link to the publication
  3. Chitosan-based matrixes for recombinant protein purification from LPS
    Authors:
    Monika Sikora, Barbara Maciejewska, Zuzanna Drulis-Kawa, Samuel M. Hudson, Jacek Semaniak, Maria Wiśniewska-Wrona, Michał Arabski
    Academic press:
    Biochemical Engineering Journal (rok: 2024, tom: 109212, strony: 45308), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bej.2023.109212 - link to the publication
  4. Dendritic systems for bacterial outer membrane disruption as a method of overcoming bacterial multidrug resistance
    Authors:
    Kinga Skrzyniarz, Dorota Kuc-Ciepluch, Magdalena Lasak, Michał Arabski, Javier Sanchez-Nieves, Karol Ciepluch
    Academic press:
    Biomaterials Science (rok: 2023, tom: 11, strony: 6421-6435), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/D3BM01255G - link to the publication
  5. Dendronized silver nanoparticles as bacterial membrane permeabilizers and their interactions with P. aeruginosa lipopolysaccharides, lysozymes, and phage-derived endolysins
    Authors:
    Karol Ciepluch, Kinga Skrzyniarz, Andrea Barrios-Gumiel, Sara Quintana, Javier Sánchez-Nieves, F. Javier de la Mata, Barbara Maciejewska, Zuzanna Drulis-Kawa, Michał Arabski
    Academic press:
    Frontiers in Microbiology (rok: 2019, tom: 10, strony: 45302), Wydawca: Frontiers Media S.A.
    Status:
    Published
    DOI:
    10.3389/fmicb.2019.02771 - link to the publication
  6. The antibacterial effect of PEGylated carbosilane dendrimers on P. aeruginosa alone and in combination with phage-derived endolysin
    Authors:
    Sara Quintana-Sanchez, Natalia Gómez-Casanova, Javier Sánchez-Nieves, Rafael Gómez, Jarosław Rachuna, Sławomir Wąsik, Jacek Semaniak, Barbara Maciejewska, Zuzanna Drulis-Kawa, Karol Ciepluch, F. Javier de la Mata, Michał Arabski
    Academic press:
    International Journal of Molecular Sciences (rok: 2022, tom: 23, strony: 45309), Wydawca: MDPI AG, St. Alban-Anlage 66 , 4052 Basel, Switzerland
    Status:
    Published
    DOI:
    10.3390/ijms23031873 - link to the publication
  7. The effect of surface modification of dendronized gold nanoparticles on activation and release of pyroptosis-inducing pro-inflammatory cytokines in presence of bacterial lipopolysaccharide in monocytes
    Authors:
    Dorota Kuc-Ciepluch, Michał Gorzkiewicz, Elżzbieta Pędziwiatr-Werbicka, Andrea Barrios-Gumiel, Magdalena Lasak, Aleksandra Mariak, Krzysztof Sztandera, Rafael Gomez, F. Javier de la Mata, Anna Lankoff, Barbara Klajnert-Maculewicz, Maria Bryszewska, Michał Arabski, Karol Ciepluch
    Academic press:
    Colloids and Surfaces B: Biointerfaces (rok: 2022, tom: 217, strony: 45300), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.colsurfb.2022.112652 - link to the publication
  8. Rodzina białek gazdermin jako czynnik permeabilizujący błonę komórkową w procesie pyroptozy
    Authors:
    Dorota Kuc-Ciepluch, Karol Ciepluch, Michał Arabski
    Academic press:
    Postępy Higieny i Medycyny Doświadczalnej (rok: 2021, tom: 75, strony: 337-334), Wydawca: Index Copernicus International
    Status:
    Published
    DOI:
    10.5604/01.3001.0014.8985 - link to the publication

O Narodowym Centrum Nauki

Finansowanie nauki

Współpraca zagraniczna

Centrum prasowe

Deklaracja dostępności
Przetwarzanie danych osobowych
Polityka cookies

Partnerzy