Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

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Structural and functional characterization of SlpA the main S-layer protein of Deinococcus radiodurans

2017/26/E/NZ1/00344

Keywords:

Deinoxanthin Bacterial surface layer SlpA Deinococcus radiodurans Photoprotection

Descriptors:

  • NZ1_1: Molecular biology
  • NZ1_4: Structural biology
  • NZ1_3: Biophysics

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Szkoła Główna Gospodarstwa Wiejskiego w Warszawie, Instytut Biologii

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Dario Piano 

Number of co-investigators in the project: 3

Call: SONATA BIS 7 - announced on 2017-06-14

Amount awarded: 1 485 300 PLN

Project start date (Y-m-d): 2018-07-02

Project end date (Y-m-d): 2022-01-01

Project duration:: 42 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. FPLC (Fast Protein Liquid Chromatography) (30 000 PLN)
  2. Huśtawki laboratoryjnej (3 900 PLN)
  3. Inkubator do krystalizacji białka (15 000 PLN)
  4. Millipore 8200 stirred cell (7 500 PLN)
  5. Zasilacza (2 400 PLN)
  6. pH-meter (4 428 PLN)
  7. Inkubator (30 000 PLN)
  8. Kolumna do sączenia molekularnego: Superose 6 10/300 GL (8 500 PLN)
  9. Wirówka laboratoryjna (100 000 PLN)
  10. Bench centrifuge.
  11. Wagi analitycznej (7 200 PLN)
  12. Mikroskop stereoskopowy (4 000 PLN)
  13. Planar Lipid Bilayer Work station.

Information in the final report

  • Publication in academic press/journals (4)
  1. Photophysics of deinoxanthin, the keto-carotenoid bound to the main S-layer unit of Deinococcus radiodurans
    Authors:
    František Adamec, Domenica Farci, David Bína, Radek Litvín, Tuhin Khan, Marcel Fuciman, Dario Piano, Tomáš Polívka
    Academic press:
    Photochemical & Photobiological Sciences (rok: 2020, tom: 19, strony: 495-503), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/d0pp00031k - link to the publication
  2. The Role of Iron and Copper on the Oligomerization Dynamics of DR_2577, the Main S-Layer Protein of Deinococcus radiodurans
    Authors:
    Domenica Farci, Giulia Guadalupi, Katarzyna Bierła, Ryszard Lobinski and Dario Piano
    Academic press:
    Frontiers in Microbiology (rok: 2019, tom: 10, 1450, strony: 45299), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fmicb.2019.01450 - link to the publication
  3. Structural analysis of the architecture and in situ localization of the main S-layer complex in Deinococcus radiodurans
    Authors:
    Farci D, Kereïche S, Pangeni S, Haniewicz P, Bodrenko IV, Ceccarelli M, Winterhalter M, Piano D.
    Academic press:
    Structure (rok: 2021, tom: 29, strony: 1279-1285.e3.), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.str.2021.06.014. - link to the publication
  4. Structural insights into the main S-layer unit of Deinococcus radiodurans reveal a massive protein complex with porin-like features.
    Authors:
    Farci D, Aksoyoglu MA, Farci SF, Bafna JA, Bodrenko I, Ceccarelli M, Kirkpatrick J, Winterhalter M, Kereïche S, Piano D
    Academic press:
    Journal of Biological Chemistry (rok: 2020, tom: 295, strony: 4224-4236), Wydawca: ASBMB Publications
    Status:
    Published
    DOI:
    10.1074/jbc.RA119.012174 - link to the publication

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