Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Molecular mechanisms of selective RNA loading into exosomes

2016/23/B/NZ1/02975

Keywords:

RNA miRNA exosomes membranes membrane rafts

Descriptors:

  • NZ1_3: Biophysics
  • NZ1_1: Molecular biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Opolski, Wydział Przyrodniczo-Techniczny

woj. opolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Tadeusz Janas 

Number of co-investigators in the project: 5

Call: OPUS 12 - announced on 2016-09-15

Amount awarded: 791 400 PLN

Project start date (Y-m-d): 2017-08-04

Project end date (Y-m-d): 2022-02-03

Project duration:: 54 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. System oczyszczania wody.
  2. cyrkulator grzewczo-chłodzący.
  3. Mikrowirówka z chłodzeniem.
  4. Termocykler do Real-Time PCR wraz z komputerem i oprogramowaniem (150 000 PLN)
  5. Termomikser z chłodzeniem i wkładem na probówki.
  6. Spektrofluorymetr UV+VIS z przystawkami i komputerem z drukarka/oprogramowaniem (150 000 PLN)

Information in the final report

  • Publication in academic press/journals (7)
  • Articles in post-conference publications (5)
  1. Role of RNA Motifs in RNA Interaction with Membrane Lipid Rafts: Implications for Therapeutic Applications of Exosomal RNAs
    Authors:
    Mańka R., Janas P., Sapoń K., Janas T., Janas T.
    Academic press:
    International Journal of Molecular Sciences (rok: 2021, tom: 22, strony: article no. 9416, pp. 1-11), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms22179416 - link to the publication
  2. Specific binding of VegT mRNA localization signal to membranes in Xenopus oocytes
    Authors:
    Tadeusz Janas, Karolina Sapoń, Teresa Janas, Michael Yarus
    Academic press:
    BBA-Mol Cell Res (rok: 2021, tom: 1868, strony: article no. 118952, pp. 1-11), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbamcr.2021.118952 - link to the publication
  3. Binding of RNA aptamers to membrane lipid rafts: implications for exosomal miRNAs transfer from cancer to immune cell
    Authors:
    Janas T, Janas P, Sapoń K, Janas T
    Academic press:
    Int J Mol Sci (rok: 2020, tom: 21, strony: article no. 8503, pp. 1-13), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21228503 - link to the publication
  4. Exosome-associated polysialic acid modulates membrane potentials, membrane thermotropic properties, and raft-dependent interactions between vesicles
    Authors:
    Sapoń K., Gawrońska I., Janas T., Sikorski A.F., Janas T.
    Academic press:
    FEBS Lett. (rok: 2020, tom: 594, strony: 1685-1697), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/1873-3468.13785 - link to the publication
  5. Cholera toxin subunit B for sensitive and rapid determination of exosomes by gel filtration
    Authors:
    Sapoń K., Maziarz D., Janas T., Sikorski A.F., Janas T.
    Academic press:
    Membranes (rok: 2020, tom: 10, strony: article no. 172, pp. 1-9), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/membranes10080172 - link to the publication
  6. The role of lipid rafts in vesicle formation
    Authors:
    Karolina Sapoń , Rafał Mańka, Teresa Janas and Tadeusz Janas
    Academic press:
    Journal of Cell Science (rok: 2023, tom: 136, strony: ), Wydawca: The Company of Biologists, UK
    Status:
    Accepted for publication
    DOI:
    10.1242/jcs.260887 - link to the publication
  7. Selection of Membrane RNA Aptamers to Amyloid Beta Peptide: Implications for Exosome-Based Antioxidant Strategies
    Authors:
    Janas Teresa, Sapoń Karolina, Stowell Michael H.B., Janas Tadeusz
    Academic press:
    International Journal of Molecular Sciences (rok: 2019, tom: 20, strony: 45307), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms20020299 - link to the publication
  1. Modulation of exosome membrane potential after treatment with endo-neuraminidase
    Authors:
    K. Sapoń, I. Gawrońska, T. Janas, T. Janas
    Conference:
    The 5th Warsaw Conference on Perspectives of Molecular Oncology: Molecular Immunology of Cancer (rok: 2020, ), Wydawca: Narodowy Instytut Onkologii
    Data:
    konferencja 23-24.09.2020
    Status:
    Published
  2. Binding of Cholera toxin subunit B to exosomal liposomes
    Authors:
    K. Sapoń, D. Maziarz, T. Janas, T. Janas
    Conference:
    The 5th Warsaw Conference on Perspectives of Molecular Oncology: Molecular Immunology of Cancer (rok: 2020, ), Wydawca: Narodowy Instytut Onkologii
    Data:
    konferencja 23-24.09.2020
    Status:
    Published
  3. The role of hydrophobic amino acids in binding of RNA to RBPs
    Authors:
    T. Janas, K. Sapoń, K.M. Drab, T. Janas
    Conference:
    The 5th Warsaw Conference on Perspectives of Molecular Oncology: Molecular Immunology of Cancer (rok: 2020, ), Wydawca: Narodowy Instytut Onkologii
    Data:
    konferencja 23-24-09.2020
    Status:
    Published
  4. Mechanisms of selective RNA loading into exosomes
    Authors:
    Teresa Janas, Pawel Janas, Karolina Sapoń, Tadeusz Janas
    Conference:
    The 5th Warsaw Conference on Perspectives of Molecular Oncology: Molecular Immunology of Cancer (rok: 2020, ), Wydawca: Narodowu Instytut Onkologii
    Data:
    konferencja 23-24.09.2020
    Status:
    Published
  5. RNA aptamers containing EXO motifs exhibit enhanced affinity for membrane rafts
    Authors:
    T. Janas, K. Sapoń, R. Mańka, T. Janas
    Conference:
    The 5th Warsaw Conference on Perspectives of Molecular Oncology: Molecular Immunology of Cancer (rok: 2020, ), Wydawca: Narodowy Instytut Onkologii
    Data:
    konferencja 23-24.09.2020
    Status:
    Published

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