Application of LCMS to quantitative analysis of composition of in vitro transcribed RNAs containing chemically modified adenine nucleotides
2016/23/N/ST4/03186
Keywords
Descriptors
- ST4_3: Metody spektroskopowe i spektrometryczne
- ST4_6: Chemia analityczna
- NZ1_1: Biologia molekularna
Panel
ST4 – Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development
Host institution
Uniwersytet Warszawski, Wydział Fizyki
Voivodeship: mazowieckie
Principal investigator (from the host institution)
Number of co-investigators in the project: 2
Call
PRELUDIUM 12 – announced on 2016-09-15
- Amount awarded
- 99 960 PLN
- Project start date
- 2017-07-27
- Project end date
- 2019-07-26
- Project duration
- 24 months (the same as in the proposal)
- Project status
- Project settled
Project description
Download the project description in a pdf file
Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.
Information in the final report
Publication in academic press/journals (2)
-
ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions
- Authors:
- Przemysław Wanat, Renata Kasprzyk, Michał Kopciał, Paweł J. Sikorski, Dominika Strzelecka, Jacek Jemielity, Joanna Kowalska
- Academic press:
- Chemical Communications (year: 2018, volume: 54, pages: 9773-9776), Publisher: The Royal Society of Chemistry
- Status:
- Published
- DOI:
- 10.1039/c8cc04968h – link to the publication
-
Phosphodiester modifications in mRNA polyA tail prevent deadenylation without compromising protein expression
- Authors:
- Dominika Strzelecka, Miroslaw Smietanski, Pawel J. Sikorski, Marcin Warminski, Joanna Kowalska, Jacek Jemielity
- Academic press:
- RNA (year: 2020, volume: in press, pages: in press), Publisher: Cold Spring Harbor Laboratory Press
- Status:
- Published
- DOI:
- 10.1261/rna.077099.120 – link to the publication
Articles in post-conference publications (4)
-
Analysis of thiophosphate RNA modification using LC-MS
- Authors:
- D. Strzelecka, M. Smietanski, M. Warminski, P. Sikorski, J. Jemielity, J.Kowalska
- Conference:
- 6th Conference of Polish Mass Spectrometry Society (year: 2018, pages: 52), Publisher: Institute of Organic Chemistry, Polish Academy of Sciences
- Date:
- conference 23-26.04.2018
- Status:
- Published
-
DEVELOPMENT OF THE LC-MS METHOD FOR THE ANALYSIS AND CHARACTERIZATION CHEMICALLY MODIFIED RNA
- Authors:
- D. Strzelecka, M. Smietanski, M. Warminski, P. Sikorski, J. Jemielity, J.Kowalska
- Conference:
- 22nd INTERNATIONAL MASS SPECTROMETRY CONFERENCE (IMSC) (year: 2018, pages: 245), Publisher: International Mass Spectrometry Foundation
- Date:
- conference 26-31.08.2018
- Status:
- Published
-
LC-MS/MS-based analysis of mRNA 5' cap metabolism and its analogs in cytoplasmic extracts of mammalian cells
- Authors:
- D. Strzelecka, M. Bednarczyk, K. Kaczmarska, P. Sikorski, J. Jemielity, J. Kowalska
- Conference:
- Young Scientist Symposium 12th Edition (year: 2019, pages: 79), Publisher: Institut Europeen de Chimie et Biologie
- Date:
- conference 23-24.05.2019
- Status:
- Published
-
Development of the LC-MS method for the analysis and characterization chemically modified RNA with therapeutic potential
- Authors:
- D. Strzelecka, M. Smietanski, M. Warminski, P. Sikorski, J. Jemielity, J.Kowalska
- Conference:
- Young Scientist Symposium 11th Edition (year: 2018, pages: 30), Publisher: Institut Europeen de Chimie et Biologie
- Date:
- conference 24-25.05.2018
- Status:
- Published