Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The mechanism of regioselective oxidative dehydrogenation of 3-ketosteroids catalyzed by D1-cholest-4-en-3-one dehydrogenase from Sterolibacterium denitrificans

2016/21/B/ST4/03798

Keywords:

flavin enzymes oxidative dehydrogenastion molecular modeling kinetic test point mutation

Descriptors:

  • ST4_15: Colloid chemistry
  • ST4_14: Catalysis
  • NZ9_9: Environmental biotechnology

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Instytut Katalizy i Fizykochemii Powierzchni im. Jerzego Habera Polskiej Akademii Nauk

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Maciej Szaleniec 

Number of co-investigators in the project: 5

Call: OPUS 11 - announced on 2016-03-15

Amount awarded: 970 840 PLN

Project start date (Y-m-d): 2017-02-23

Project end date (Y-m-d): 2021-05-22

Project duration:: 51 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Chromatograf FPLC do preparatywnego oczyszczania białek (280 000 PLN)
  2. 4-kanałowy kontroler do fermentora (25 000 PLN)
  3. Komputer sterujący PC.
  4. Komputer PC do modelowania molekularnego (4 500 PLN)
  5. Spektrofotometr UV-vis z przystawką temperaturową (100 000 PLN)
  6. licencja Gaussian View W6.

Information in the final report

  • Publication in academic press/journals (6)
  • Book publications / chapters in book publications (1)
  1. The efficient Δ1-dehydrogenation of a wide spectrum of 3-ketosteroids in a broad pH range by 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans
    Authors:
    Agnieszka M. Wojtkiewicz, Patrycja Wójcik, Magdalena Procner, Monika Flejszar, Maria Oszajca, Mateusz Hochołowski, Mateusz Tataruch, Beata Mrugała, Tomasz Janeczko, Maciej Szaleniec
    Academic press:
    The Journal of Steroid Biochemistry and Molecular Biology (rok: 2020, tom: 202, strony: 105731), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jsbmb.2020.105731 - link to the publication
  2. Application of Immobilized Cholest-4-En-3-One D1- Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids
    Authors:
    M. Tataruch, P. Wójcik, A. M. Wojtkiewicz, K. Zaczyk, K. Szymańska, M. Szaleniec
    Academic press:
    Catalysts (rok: 2020, tom: 10(12), strony: 1460), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/catal10121460 - link to the publication
  3. Enzymatic Δ1-dehydrogenation of 3-ketosteroids – reconciliation of kinetic isotope effects with the reaction mechanism
    Authors:
    Michał Glanowski, Patrycja Wójcik, Magdalena Procner, Maria Oszajca, Katarzyna Świderek, Vicent Moliner, Andrzej J. Bojarski, Maciej Szaleniec
    Academic press:
    ACS Catalysis (rok: 2021, tom: 11, strony: 8211−8225), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acscatal.1c01479 - link to the publication
  4. Structure and reaction mechanism of 3-ketosteroid Δ1-dehydrogenase from Sterolibacterium denitrificans
    Authors:
    Patrycja Wójcik, Michał Glanowski, Beata Mrugała, Magdalena Procner, Olga Zastawny, Monika Flejszar, Katarzyna Kurpiewska, Ewa Niedziałkowska, Wladek Minor, Maria Oszajca, Andrzej J. Bojarski, Agnieszka M. Wojtkiewicz, Maciej Szaleniec
    Academic press:
    ACS Catalysis (rok: 2022, ), Wydawca: ACS
    Status:
    Submitted
    DOI:
    brak - link to the publication
  5. Universal Capability of 3-Ketosteroid Δ1- Dehydrogenases to Catalyze Δ1-Dehydrogenation of C17- Substituted Steroids
    Authors:
    P. Wójcik, M. Glanowski, A. M. Wojtkiewicz, A. Rohman, M. Szaleniec
    Academic press:
    Microbial Cell Factories (rok: 2021, tom: 20, strony: 119), Wydawca: BMC
    Status:
    Published
    DOI:
    10.1186/s12934-021-01611-5 - link to the publication
  6. Investigation of quaternary structure of aggregating 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans: In the pursuit of consensus of various biophysical techniques
    Authors:
    Kamila Sofińska, Agnieszka M. Wojtkiewicz, Patrycja Wójcik, Olga Zastawny, Maciej Guzik, Agnieszka Winiarska, Piotr Waligórski, Michał Cieśla, Jakub Barbasz, Maciej Szaleniec
    Academic press:
    BBA - General Subjects (rok: 2019, tom: 1863, strony: 1027-1039), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagen.2019.03.009 - link to the publication
  1. Model setup and procedures for prediction of enzyme reaction kinetics with QM-only and QM:MM approaches
    Authors:
    M. Glanowski, S. Kachhap, T. Borowski, M. Szaleniec
    Book:
    'Computational Methods for Estimating the Kinetics Parameters of Biological Systems' in 'Methods in Molecular Biology' (rok: 2022, tom: 2385, strony: 175-236), Wydawca: Springer US, Humana, New York, NY.
    Status:
    Published

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