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The mechanism of regioselective oxidative dehydrogenation of 3-ketosteroids catalyzed by D1-cholest-4-en-3-one dehydrogenase from Sterolibacterium denitrificans

2016/21/B/ST4/03798

Keywords

Descriptors

  • ST4_15: Chemia teoretyczna i obliczeniowa
  • ST4_14: Reakcje chemiczne: mechanizmy, termodynamika, kinetyka i kataliza
  • NZ9_9: Biotechnologia środowiskowa

Panel

ST4 – Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution

Instytut Katalizy i Fizykochemii Powierzchni im. Jerzego Habera PAN

Voivodeship: małopolskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Maciej Szaleniec

Number of co-investigators in the project: 5

Call

OPUS 11 – announced on 2016-03-15

Amount awarded
970 840 PLN
Project start date
2017-02-23
Project end date
2021-05-22
Project duration
51 months (the same as in the proposal)
Project status
Project settled

Project description

Download the project description in a pdf file

Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. 4-kanałowy kontroler do fermentora. Cost: 25 000 PLN
  2. Chromatograf FPLC do preparatywnego oczyszczania białek. Cost: 280 000 PLN
  3. Komputer PC do modelowania molekularnego. Cost: 4 500 PLN
  4. Komputer sterujący PC.
  5. Spektrofotometr UV-vis z przystawką temperaturową. Cost: 100 000 PLN
  6. licencja Gaussian View W6.

Information in the final report

Publication in academic press/journals (5)

  1. Universal Capability of 3-Ketosteroid Δ1- Dehydrogenases to Catalyze Δ1-Dehydrogenation of C17- Substituted Steroids

    Authors:
    P. Wójcik, M. Glanowski, A. M. Wojtkiewicz, A. Rohman, M. Szaleniec
    Academic press:
    Microbial Cell Factories (year: 2021, volume: 20, pages: 119), Publisher: BMC
    Status:
    Published
    DOI:
    10.1186/s12934-021-01611-5 – link to the publication

  2. Investigation of quaternary structure of aggregating 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans: In the pursuit of consensus of various biophysical techniques

    Authors:
    Kamila Sofińska, Agnieszka M. Wojtkiewicz, Patrycja Wójcik, Olga Zastawny, Maciej Guzik, Agnieszka Winiarska, Piotr Waligórski, Michał Cieśla, Jakub Barbasz, Maciej Szaleniec
    Academic press:
    BBA - General Subjects (year: 2019, volume: 1863, pages: 1027-1039), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagen.2019.03.009 – link to the publication

  3. Application of Immobilized Cholest-4-En-3-One D1- Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids

    Authors:
    M. Tataruch, P. Wójcik, A. M. Wojtkiewicz, K. Zaczyk, K. Szymańska, M. Szaleniec
    Academic press:
    Catalysts (year: 2020, volume: 10(12), pages: 1460), Publisher: MDPI
    Status:
    Published
    DOI:
    10.3390/catal10121460 – link to the publication

  4. The efficient Δ1-dehydrogenation of a wide spectrum of 3-ketosteroids in a broad pH range by 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans

    Authors:
    Agnieszka M. Wojtkiewicz, Patrycja Wójcik, Magdalena Procner, Monika Flejszar, Maria Oszajca, Mateusz Hochołowski, Mateusz Tataruch, Beata Mrugała, Tomasz Janeczko, Maciej Szaleniec
    Academic press:
    The Journal of Steroid Biochemistry and Molecular Biology (year: 2020, volume: 202, pages: 105731), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jsbmb.2020.105731 – link to the publication

  5. Enzymatic Δ1-dehydrogenation of 3-ketosteroids – reconciliation of kinetic isotope effects with the reaction mechanism

    Authors:
    Michał Glanowski, Patrycja Wójcik, Magdalena Procner, Maria Oszajca, Katarzyna Świderek, Vicent Moliner, Andrzej J. Bojarski, Maciej Szaleniec
    Academic press:
    ACS Catalysis (year: 2021, volume: 11, pages: 8211−8225), Publisher: ACS
    Status:
    Published
    DOI:
    10.1021/acscatal.1c01479 – link to the publication

Book publications / chapters in book publications (1)

  1. Model setup and procedures for prediction of enzyme reaction kinetics with QM-only and QM:MM approaches

    Authors:
    M. Glanowski, S. Kachhap, T. Borowski, M. Szaleniec
    Book:
    'Computational Methods for Estimating the Kinetics Parameters of Biological Systems' in 'Methods in Molecular Biology' (year: 2022, volume: 2385, pages: 175-236), Publisher: Springer US, Humana, New York, NY.
    Status:
    Published

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Graphic design and implementation: Jakub Piotrowski