Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Silicon biosensing surfaces functionalized with many molecules: direct analysis of antibody orientation and antigens binding efficiency with secondary ion mass spectrometry

2016/21/N/ST5/00880

Keywords:

antibody orientation immunoassay self-assambly monolayers biosensing surfaces TOF-SIMS

Descriptors:

  • ST5_3: Surface modification

Panel:

ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution :

Uniwersytet Jagielloński, Wydział Fizyki, Astronomii i Informatyki Stosowanej

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

Katarzyna Gajos 

Number of co-investigators in the project: 3

Call: PRELUDIUM 11 - announced on 2016-03-15

Amount awarded: 149 600 PLN

Project start date (Y-m-d): 2017-03-20

Project end date (Y-m-d): 2021-01-19

Project duration:: 46 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Moduł umożliwiający przesuw i skanowanie w wybranym kierunku do spektrometru odbiciowego światła białego FRBasics (44 000 PLN)
  2. Wytrząsarka typu Vortex (2 575 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  1. Surface density dependent orientation and immunological recognition of antibody on silicon: TOF-SIMS and surface analysis of two covalent immobilization methods
    Authors:
    Katarzyna Gajos, Klaudia Szafraniec, Panagiota Petrou, Andrzej Budkowski
    Academic press:
    Applied Surface Science (rok: 2020, tom: 518, strony: article number 146269), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.apsusc.2020.146269 - link to the publication
  2. Using a lactadherin-immobilized silicon surface for capturing and monitoring plasma microvesicles as a foundation for diagnostic device development
    Authors:
    Agnieszka Kamińska, Katarzyna Gajos, Olga Woźnicka, Anna Dłubacz, Magdalena E. Marzec, Andrzej Budkowski, Ewa Ł. Stępień
    Academic press:
    Analytical and Bioanalytical Chemistry (rok: 2020, tom: 412, strony: 8093-8106), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-020-02938-5 - link to the publication
  3. Protein adsorption/desorption and antibody binding stoichiometry on silicon interferometric biosensors examined with TOF-SIMS
    Authors:
    Katarzyna Gajos, Andrzej Budkowski, Panagiota Petrou, Varvara Pagkali, Kamil Awsiuk, Jakub Rysz, Andrzej Bernasik, Konstantinos Misiakos, Ioannis Raptis, Sotirios Kakabakos
    Academic press:
    Applied Surface Science (rok: 2018, tom: 444, strony: 187-196), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.apsusc.2018.03.029 - link to the publication
  4. Controlling orientation, conformation, and biorecognition of proteins on silane monolayers, conjugate polymers, and thermo-responsive polymer brushes: investigations using TOF-SIMS and principal component analysis
    Authors:
    Katarzyna Gajos, Kamil Awsiuk, Andrzej Budkowski
    Academic press:
    Colloid and Polymer Science (rok: 2021, tom: 299, strony: 385-405), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00396-020-04711-7 - link to the publication

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