Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Toxicity of amphotericin B in the context of the plasma membrane lipid reorganization by the activity of ABC transporters in mammalian cells.

2016/21/B/NZ3/00343

Keywords:

ABC transporters plasma membrane lateral organization amphotericin B fluorescence spectroscopy molecular spectroscopy

Descriptors:

  • NZ3_1: Cell biology
  • NZ1_3: Biophysics
  • NZ1_2: Biochemistry

Panel:

NZ3 - Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution :

Uniwersytet Wrocławski, Wydział Biotechnologii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Tomasz Trombik 

Number of co-investigators in the project: 6

Call: OPUS 11 - announced on 2016-03-15

Amount awarded: 635 340 PLN

Project start date (Y-m-d): 2017-01-12

Project end date (Y-m-d): 2020-07-11

Project duration:: 42 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Dewar do przechowywania komórek LS750.
  2. System do elektrotransferu białek typu "semi-dry" (10 000 PLN)
  3. Kontroler temperatury CL-100.
  4. Wytrząsarka kołyskowa (7 000 PLN)
  5. Łażnia wodna (8 000 PLN)
  6. System do elektroforezy żelowej (SDS-PAGE) białek wraz z zasilaniem (10 000 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  1. ABCA1 transporter reduces amphotericin B cytotoxicity in mammalian cells
    Authors:
    Wu A, Grela E, Wójtowicz K, Filipczak N, Hamon Y, Luchowski R, Grudziński W, Raducka-Jaszul O, Gagoś M, Szczepaniak A, Chimini G, Gruszecki WI, Trombik T
    Academic press:
    Cellular and Molecular Life Sciences (rok: 2019, tom: 76(24), strony: 4979-4994), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00018-019-03154-w - link to the publication
  2. Do ABC transporters regulate plasma membrane organization?
    Authors:
    Ambroise Wu, Karolina Wojtowicz, Stephane Savary, Yannick Hamon, Tomasz Trombik
    Academic press:
    Cellular and Molecular Biology Letters (rok: 2020, tom: 25, strony: 0), Wydawca: BMC Springer Nature
    Status:
    Published
    DOI:
    10.1007/s00018-019-03154-w. - link to the publication
  3. Spot variation Fluorescence Correlation Spectroscopy for analysis the diffusion of molecules at the plasma membrane of living cell
    Authors:
    Sébastien Mailfert, Karolina Wojtowicz, Sophie Brustlein, Ewa Blaszczak, Marcin Łukaszewicz, Didier Marguet, Tomasz Trombik
    Academic press:
    Journal of Visualized Experiments (rok: 2020, tom: -, strony: -), Wydawca: JoVE
    Status:
    Published
    DOI:
    10.3791/61823 - link to the publication
  4. Molecular Diffusion of ABCA1 at the Cell Surface of Living Cells Assessed by svFCS
    Authors:
    Olga Raducka-Jaszul, Karolina Wójtowicz, Aleksander F. Sikorski, Giovanna Chimini, Yannick Hamon, Tomasz Trombik
    Academic press:
    Membranes (rok: 2021, tom: 11(7) 498, strony: -), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/membranes11070498 - link to the publication

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