Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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A new approach to the identification of pathogens using hyphenated separation techniques (CE-LIF-MALDI-TOF-MS, LCxLC-MS/MS, GCxGC-Q-TOF) for personalized medical diagnostics

2016/21/B/ST4/02130

Keywords:

capillary electrophoresis chromatography surgical wound infections microorganisms mass spectrometry

Descriptors:

  • ST4_6: Instrumental methods in chemistry
  • ST4_3: Molecular architecture and structure

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Mikołaja Kopernika, Wydział Chemii

woj. kujawsko-pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Małgorzata Szultka-Młyńska 

Number of co-investigators in the project: 6

Call: OPUS 11 - announced on 2016-03-15

Amount awarded: 1 041 600 PLN

Project start date (Y-m-d): 2017-02-23

Project end date (Y-m-d): 2020-10-22

Project duration:: 44 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Komputer (3 000 PLN)
  2. Drukarka (1 000 PLN)
  3. Urządzenie do automatycznej ekstrakcji lotnych związków oorganicznych i przygotowania próbek.
  4. Detektor LIF - wzbudzona laserowo fluorescencja (230 000 PLN)
  5. Oprogramowanie do identyfikacji bakterii z biblioteką widm IC MALDI (intact cells MALDI) z możliwością jej rozbudowy (Biotyper). Producent: firma Bruker, Niemcy (220 000 PLN)

Information in the final report

  • Publication in academic press/journals (14)
  • Articles in post-conference publications (20)
  1. Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples
    Authors:
    Katarzyna Pauter, Małgorzata Szultka-Młyńska, Bogusław Buszewski
    Academic press:
    Molecules (rok: 2020, tom: 25, strony: 15342), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules25112556 - link to the publication
  2. Problems with identification and distinguishing salivary bacteria - multi-instrumental approach
    Authors:
    M. Złoch, A. Rodzik, O. Pryshchepa, K. Pauter, M. Szultka-Młyńska, A. Rogowska, W. Kupczyk, P. Pomastowski, B. Buszewski
    Academic press:
    Future Microbiology (rok: 2020, tom: 15, strony: 1157-1171), Wydawca: future science group
    Status:
    Published
    DOI:
    10.2217/fmb-2020-0036 - link to the publication
  3. Application of solid phase microextraction followed by liquid chromatography-mass spectrometry in the determination of antibiotic drugs and their metabolites in human whole blood and tissue samples
    Authors:
    M. Szultka-Młyńska, P. Pomastowski, B. Buszewski
    Academic press:
    Journal of Chromatography B (rok: 2018, tom: 1086, strony: 153-165), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jchromb.2018.04.013 - link to the publication
  4. The study of the molecular mechanism of Lactobacillus paracasei clumping via divalent metal ions by electrophoretic separation
    Authors:
    A. Król-Górniak, P. Pomastowski, V. Railean-Plugaru, Petar Žuvela, Ming Wah Wong, K. Pauter, M. Szultka-Młyńska, B. Buszewski
    Academic press:
    Journal of Chromatography A (rok: 2021, tom: 1652, strony: 45303), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.chroma.2021.462127 - link to the publication
  5. Identification of Microorganisms by Modern Analytical Techniques
    Authors:
    BOGUSŁAW BUSZEWSKI, AGNIESZKA ROGOWSKA, PAWEŁ POMASTOWSKI, MICHAŁ ZŁOCH, and VIORICA RAILEAN-PLUGARU
    Academic press:
    JOURNAL OF AOAC INTERNATIONAL (rok: 2017, tom: VOL. 100, No. 6, strony: 1607-1623), Wydawca: AOAC International
    Status:
    Published
    DOI:
    10.5740/jaoacint.17-0207 - link to the publication
  6. The effect of growth medium on an Escherichia coli pathway mirrored into GC/MS profiles
    Authors:
    Ileana-Andreea Ratiu, Tomasz Ligor, Victor Bocos-Bintintan, Hossam Al-Suod, Tomasz Kowalkowski, Katarzyna Rafińska and Bogusław Buszewski
    Academic press:
    Journal of Breath Research (rok: 2017, tom: 11 (3), strony: 45303), Wydawca: Institute of Physics Publishing
    Status:
    Published
    DOI:
    10.1088/1752-7163/aa7ba2 - link to the publication
  7. Capillary electrophoresis/mass spectrometry for determination and identification of selected antibiotic drugs and their metabolites in human urine
    Authors:
    Katarzyna Pauter, Małgorzata Szultka-Młyńska, Michał Szumski, Anna Król-Górniak, Paweł Pomastowski, Bogusław Buszewski
    Academic press:
    Electrophoresis (rok: 2021, tom: -, strony: -), Wydawca: Wiley-VCH
    Status:
    Submitted
    DOI:
    brak - link to the publication
  8. Electrophoretic Determination of Lactococcus lactis Modified by Zinc Ions
    Authors:
    B. Buszewski, A. Król, P. Pomastowski, V. Railean‑Plugaru, M. Szultka‑Młyńska
    Academic press:
    Chromatographia (rok: 2019, tom: 82, strony: 347–355), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10337-018-3665-3 - link to the publication
  9. The influence of different pH on the electrophoretic behaviour of Saccharomyces cerevisiae modified by calcium ions
    Authors:
    A. Rogowska, P. Pomastowski, M. Złoch, V. Railean-Plugaru, A. Król, K. Rafińska, M. Szultka-Młyńska, B. Buszewski
    Academic press:
    Scientific Reports (rok: 2018, tom: 5,37569444444444, strony: 45301), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-018-25024-4 - link to the publication
  10. Mass spectrometric techniques for the analysis of volatile organic compounds emitted from bacteria
    Authors:
    Ileana-Andreea Ratiu, Tomasz Ligor, Victor Bocos- Bintintan & Bogusław Buszewski
    Academic press:
    Bioanalysis (rok: 2017, tom: 9(14), strony: 1069-1092), Wydawca: Future Medicine Ltd.
    Status:
    Published
    DOI:
    10.4155/bio-2017-0051 - link to the publication
  11. Microbiology neutralization of zearalenone using Lactococcus lactis and Bifidobacterium sp.
    Authors:
    A. Król, P. Pomastowski, K. Rafińska, V. Railean-Plugaru, J. Walczak, B. Buszewski
    Academic press:
    Analytical and Bioanalytical Chemistry (rok: 2018, tom: 410, strony: 943-952), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-017-0555-8 - link to the publication
  12. Structural characterization of electrochemically and in vivo generated potential metabolites of selected cardiovascular drugs by EC-UHPLC/ESIMS using an experimental design approach
    Authors:
    M. Szultka-Młyńska, S. Bajkacz, I. Baranowska, B. Buszewski
    Academic press:
    Talanta (rok: 2018, tom: 176, strony: 262-276), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.talanta.2017.08.041 - link to the publication
  13. Temporal influence of different antibiotics onto the inhibition of Escherichia coli bacterium grown in different media
    Authors:
    Ileana Andreea Ratiu, Viorica Railean Plugaru, Pawel Pomastowski, Maciej Milanowski, Radik Mametov, Victor Bocos-Bintintan, Boguslaw Buszewski
    Academic press:
    Analytical Biochemistry (rok: 2019, tom: 585, strony: 113407), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ab.2019.113407 - link to the publication
  14. The effect of biosilver nanoparticles on different bacterial strains' metabolism reflected in their VOCs profiles
    Authors:
    B. Buszewski, I.A. Rațiu, M. Milanowski, P. Pomastowski, T. Ligor
    Academic press:
    Journal of Breath Research (rok: 2018, tom: 12 (2), strony: 45299), Wydawca: Institute of Physics Publishing
    Status:
    Published
    DOI:
    10.1088/1752-7163/aa820f - link to the publication
  1. APPLICATION OF LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY FOR ELECTROCHEMICAL OXIDATION OF ANTIBIOTICS IN COMPARISON TO IN VITRO AND IN VIVO METHODS
    Authors:
    M. Szultka-Młyńska, K. Pauter, W. Kupczyk, B. Buszewski
    Conference:
    48TH International Symposium on High-Performance Liquid Phase Separations and Related Techniques (rok: 2019, ), Wydawca: University of Milano-Bicocca, Milan
    Data:
    konferencja 16-20.VI.2019
    Status:
    Published
  2. Development of HPLC-DAD method for determination and identification of selected antibiotics and their metabolites for biomedical purposes
    Authors:
    K. Pauter, M.Szultka Młyńska, J. Walczak, B.Buszewski
    Conference:
    11. Kongres Societas Humboldtiana Polonorum (rok: 2019, ), Wydawca: Organizator konferencji
    Data:
    konferencja 12-15.IX.2019
    Status:
    Published
  3. Identification of salivary bacteria using MALDI TOF MS and 16s DNA PCR approach
    Authors:
    A. Rodzik, P. Pomastowski, M. Złoch, M. Szultka-Młyńska, B. Buszewski
    Conference:
    Analytical methods to study oxidative damage, antioxidants and drugs (rok: 2018, ), Wydawca: Medical University of Białystok
    Data:
    konferencja 24-26.V.2018
    Status:
    Published
  4. APPLICATION OF EC LC-MS/MS AS A TOOL IN METABOLISM STUDIES
    Authors:
    Szultka-Młyńska M., Buszewski B.
    Conference:
    Metabolomics Circle 2019 (rok: 2019, ), Wydawca: Organizator konferencji
    Data:
    konferencja 15-16.XI.2019
    Status:
    Published
  5. CAPILLARY ELECTROPHORESIS AS A PROMISING APPROACH FOR ANTIBIOTICS AND THEIR METABOLITES DETERMINATION IN URINE SAMPLES
    Authors:
    Katarzyna Pauter, Michał Szumski, Anna Król-Górniak, Paweł Pomastowski, Małgorzata Szultka-Młyńska, Bogusław Buszewski
    Conference:
    Quo Vadis Life Sciences Conference, 2021 (rok: 2021, ), Wydawca: Organizator konferencji
    Data:
    konferencja 23-27.VI.2021
    Status:
    Published
  6. Complementary approach on bacteria and antibiotics investgation
    Authors:
    K. Pauter, M. Szultka-Młyńska, P. Pomastowski, M. Złoch, B. Buszewski
    Conference:
    15th International Students Conference "Modern Analytical Chemistry" (rok: 2019, ), Wydawca: Charles University, Faculty of Science
    Data:
    konferencja 19-20.IX.2019
    Status:
    Published
  7. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of bacteria profiles in a clinical samples
    Authors:
    M. Szultka-Młyńka, K. Pauter, D. Błońska, W. Kupczyk, B. Buszewski
    Conference:
    25th International Symposium on Separation Sciences (rok: 2019, ), Wydawca: Organizator konferencji
    Data:
    konferencja 15-18.IX.2019
    Status:
    Published
  8. The influence of pH on the electrophoretic behaviour of yeast modified by calcium ions
    Authors:
    A. Rogowska, P. Pomastowski, M. Złoch, V. Railean-Plugaru, A. Król, K. Rafińska, M. Szultka-Młyńska, B. Buszewski
    Conference:
    ISC Modern Analytical Chemistry (rok: 2018, ), Wydawca: Charles University, Faculty of Science
    Data:
    konferencja 20-21.IX.2018
    Status:
    Published
  9. Application of MALDI TOF MS technique for examination of clinical specimens on example of salivary bacteria analysis
    Authors:
    M. Złoch, O. Pryshchepa, P. Pomastowski, M. Szultka-Młyńska, B. Buszewski
    Conference:
    Metabolomics Circle 2018 (rok: 2018, ), Wydawca: Organizator konferencji
    Data:
    konferencja 26-28.X.2018
    Status:
    Published
  10. Separation of bacteria by capillary zone electrophoresis. Effect of charge distribution
    Authors:
    B. Buszewski
    Conference:
    Międzynarodowa Konferencja Chromatografia Jonowa i techniki pokrewne 2018 (rok: 2018, ), Wydawca: Organizator konferencji
    Data:
    konferencja 17-18.IV.2018
    Status:
    Published
  11. Determination of biocolloids uncontrolled clumping mechanism by capillary electrophoresis
    Authors:
    Król A., Pomastowski P., Railean-Plugaru V., Pauter K., Szultka-Młyńska M., Buszewski B.
    Conference:
    6. Konferencja Naukowa, Monitoring i analiza wody. Chromatograficzne metody oznaczania substancji o charakterze jonowym (rok: 2019, ), Wydawca: Organizator konferencji
    Data:
    konferencja 10-12.III.2019
    Status:
    Published
  12. Analysis of Lactococcus lactis modified with zinc ions by capillary electrophoresis
    Authors:
    A. Król, P. Pomastowski, V. Railean-Plugaru, B. Buszewski
    Conference:
    ISC Modern Analytical Chemistry (rok: 2018, ), Wydawca: Charles University, Faculty of Science
    Data:
    konferencja 20-21.IX.2018
    Status:
    Published
  13. ELECTROCHEMICAL SIMULATION OF BIOTRANSFORMATION REACTIONS OF DRUGS FROM DIFFERENT THERAPEUTIC GROUPS BY EC-HPLC/ESI-MS/MS
    Authors:
    M. Szultka-Młyńska, B. Buszewski
    Conference:
    Applied Natural Sciences 2019 (rok: 2019, ), Wydawca: University of SS. Cyril and Methodius in Trnava
    Data:
    konferencja 25-27.IX.2019
    Status:
    Published
  14. Effect of different types of divalent metal ions on Lactobacillus paracasei electrophoretic determination
    Authors:
    Król A., Pomastowski P., Railean-Plugaru V., Buszewski B., Szultka-Młyńska M.
    Conference:
    15th International Students Conference "Modern Analytical Chemistry" (rok: 2019, ), Wydawca: Charles University, Faculty of Science
    Data:
    konferencja 19-20.IX.2019
    Status:
    Published
  15. Electrochemistry-Mass Spectrometry for Determination of Selected Drugs and Their Electrochemical Products in Comparison to In-Vitro and In-Vivo Approaches
    Authors:
    M. Szultka-Młyńska, B. Buszewski
    Conference:
    25th International Symposium on Separation Sciences (rok: 2019, ), Wydawca: Organizator konferencji
    Data:
    konferencja 15-18.IX.2019
    Status:
    Published
  16. How capillary zone electrophoresis and related techniques can be used for identification of pathogens?
    Authors:
    P. P. Pomastowski, A. Król, M. Szultka-Młyńska, B. Buszewski
    Conference:
    42nd ISCC and 15th GC×GC Symposium (rok: 2018, ), Wydawca: Chromaleont S.r.L. Via Leonardo Sciascia snc Coop. Fede pal. B Ex Via Torrente San Licandro 98168 Messina, Italy
    Data:
    konferencja 13-18.V.2018
    Status:
    Published
  17. Salivary microbiome profiling with the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry
    Authors:
    Katarzyna Pauter, Małgorzata Szultka-Młyńska, Dominika Błońska, Wojciech Kupczyk, Bogusław Buszewski
    Conference:
    Metabolomics Circle 2019 (rok: 2019, ), Wydawca: Organizator konferencji
    Data:
    konferencja 15-16.XI.2019
    Status:
    Published
  18. Selection of experimental conditions for the identification of bacteria by MALDI-TOF MS
    Authors:
    O. Pryshchepa, M. Złoch, K. Pauter, M. Szultka-Młyńska, P. Pomastowski, B. Buszewski
    Conference:
    15th International Students Conference "Modern Analytical Chemistry" (rok: 2019, ), Wydawca: Charles University, Faculty of Science
    Data:
    konferencja 19-20.IX.2019
    Status:
    Published
  19. Separation of Lactococcus lactis modified with zinc by capillary electrophoresis
    Authors:
    A. Król, P. Pomastowski, V. Railean-Plugaru, M. Szultka-Młyńska, B. Buszewski
    Conference:
    Metabolomics Circle 2018 (rok: 2018, ), Wydawca: Organizator konferencji
    Data:
    konferencja 26-28.X.2018
    Status:
    Published
  20. What's a new in sample preparation for bioanalysis
    Authors:
    B. Buszewski, R. Gadzała-Kopciuch, M. Szultka-Młyńska
    Conference:
    Analytical methods to study oxidative damage, antioxidants and drugs (rok: 2018, ), Wydawca: Medical University of Białystok
    Data:
    konferencja 24-26.V.2018
    Status:
    Published

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