Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Construction of recombinant strain of African swine fever (ASFV) using CRISPR/Cas9 technique and examination of its biological features in vitro and in pig model.

2016/21/D/NZ6/00974

Keywords:

African swine fever recombinant ASFV strain CRISPR/Cas9 biological features

Descriptors:

  • NZ6_5: Virology
  • NZ7_1: Epidemiology
  • NZ2_1: Molecular genetics

Panel:

NZ6 - Human and animal immunology and infection: immunity, immune disorders, immunotherapy, infectious and invasive diseases, microbiology, transplantology, allergology

Host institution :

Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy

woj. lubelskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Grzegorz Woźniakowski 

Number of co-investigators in the project: 3

Call: SONATA 11 - announced on 2016-03-15

Amount awarded: 597 547 PLN

Project start date (Y-m-d): 2017-01-13

Project end date (Y-m-d): 2021-01-12

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Inkubator z dopływem dwutlenku węgla (CO2) do prowadzenia hodowli komórek. (25 000 PLN)
  2. Laboratoryjna zamrażarka niskotemperaturowa. (37 000 PLN)
  3. Mikroskop holograficzny do obrazowania i analizy komórek adherentnych bez znakowania (HoloMonitor M4) (73 800 PLN)
  4. Komputer przenośny z oprogramowaniem (Mac OS X, Microsoft Office). (4 500 PLN)

Information in the final report

  • Publication in academic press/journals (13)
  • Articles in post-conference publications (1)
  1. Blood Counts, Biochemical Parameters, Inflammatory, and Immune Responses in Pigs Infected Experimentally with the African Swine Fever Virus Isolate Pol18_28298_O111.
    Authors:
    Walczak Marek, Wasiak Magdalena, Dudek Katarzyna, Kycko Anna, Szacawa Ewelina, Olech Małgorzata, Woźniakowski Grzegorz, Szczotka-Bochniarz Anna
    Academic press:
    Viruses (rok: 2021, tom: 13 (3), strony: 521), Wydawca: MDPI, Basel, Switzerland
    Status:
    Published
    DOI:
    10.3390/v13030521 - link to the publication
  2. African Swine Fever Virus - Persistence in Different Environmental Conditions and the Possibility of its Indirect Transmission.
    Authors:
    Mazur-Panasiuk Natalia, Żmudzki Jacek, Woźniakowski Grzegorz
    Academic press:
    Journal of Veterinary Research (rok: 2019, tom: 63, strony: 303-310), Wydawca: Sciendo, National Veterinary Research Institute in Pulawy
    Status:
    Published
    DOI:
    10.2478/jvetres-2019-0058 - link to the publication
  3. African swine fever virus - The possible role of flies and other insects in virus transmission
    Authors:
    Fila Mateusz, Woźniakowski Grzegorz
    Academic press:
    Journal of Veterinary Research (rok: 2020, tom: 64, strony: 45298), Wydawca: Sciendo, National Veterinary Research Institute in Pulawy
    Status:
    Published
    DOI:
    10.2478/jvetres-2020-0001 - link to the publication
  4. Attempts at the development of a recombinant African swine fever virus strain with abrogated EP402R, 9GL, and A238L gene structure using the CRISPR/Cas9 system.
    Authors:
    Woźniakowski Grzegorz, Mazur-Panasiuk Natalia, Walczak Marek, Juszkiewicz Małgorzata, Frant Maciej, Krzysztof Niemczuk
    Academic press:
    Journal of Veterinary Research (rok: 2020, tom: 64, strony: 197-205), Wydawca: Sciendo, National Veterinary Research Institute in Pulawy
    Status:
    Published
    DOI:
    10.2478/jvetres-2020-0039 - link to the publication
  5. Vertical transmission of anti-ASFV antibodies as one of potential causes of seropositive results among young wild boar population in Poland
    Authors:
    Walczak Marek, Frant Maciej, Juszkiewicz Małgorzata, Mazur-Panasiuk Natalia, Szymankiewicz Krzesimir, Bruczyńska Małgorzata, Woźniakowski Grzegorz
    Academic press:
    Polish Journal of Veterinary Sciences (rok: 2020, tom: 23, strony: 21-25), Wydawca: Polish Academy of Sciences Committee of Veterinary Sciences and the University of Warmia and Mazury in Olsztyn.
    Status:
    Published
    DOI:
    10.24425/pjvs.2019.131415 - link to the publication
  6. Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid.
    Authors:
    Ann Sofie Olesen, Louise Lohse, Marlene Danner Dalgaard, Grzegorz Woźniakowski, Graham J.Belsham, Anette Bøtner, Thomas Bruun Rasmussen
    Academic press:
    Journal of Virological Methods (rok: 2018, tom: 261, strony: 14-16), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jviromet.2018.07.015. - link to the publication
  7. The spillover of African swine fever in western Poland revealed its estimated origin on the basis of O174L, K145R, MGF 505-5R and IGR I73R/I329L genomic sequences
    Authors:
    Mazur-Panasiuk Natalia, Walczak Marek, Juszkiewicz Małgorzata, Woźniakowski Grzegorz.
    Academic press:
    Viruses (rok: 2020, tom: 12, strony: 1094), Wydawca: MDPI, Basel, Switzerland
    Status:
    Published
    DOI:
    10.3390/v12101094 - link to the publication
  8. The unique genetic variation within the O174L gene of Polish strains of African swine fever virus facilitates tracking virus origin.
    Authors:
    Natalia Mazur-Panasiuk, Grzegorz Woźniakowski
    Academic press:
    Archives of Virology (rok: 2019, tom: 164, strony: 1667-1672), Wydawca: Springer Link
    Status:
    Published
    DOI:
    10.1007/s00705-019-04224-x - link to the publication
  9. African swine fever (ASF) and ticks. No risk of tick-mediated ASF spread in Poland and Baltic states.
    Authors:
    Frant Maciej, Woźniakowski Grzegorz, Pejsak Zygmunt
    Academic press:
    Journal of Veterinary Research (rok: 2017, tom: 61, strony: 375-380), Wydawca: De Gruyter, National Veterinary Research Institute in Pulawy
    Status:
    Published
    DOI:
    10.1515/jvetres-2017-0055 - link to the publication
  10. African swine fever virus (ASFV) in Poland: Prevalence in a wild boar population (2017–2018)
    Authors:
    Frant Maciej, Lyjak Magdalena, Bocian Łukasz, Barszcz Anna, Niemczuk Krzysztof, Wozniakowski Grzegorz
    Academic press:
    Veterinarni Medicina (rok: 2020, tom: 65, strony: 143-158), Wydawca: Open Access CAAS Agricultural Journals
    Status:
    Published
    DOI:
    10.17221/105/2019-VETMED - link to the publication
  11. Analysis of the clinical course of experimental infection with highly pathogenic African swine fever strain, isolated from an outbreak in Poland. Aspects related to the disease suspicion at the farm level.
    Authors:
    Walczak Marek, Żmudzki Jacek, Mazur-Panasiuk Natalia, Juszkiewicz Małgorzata, Woźniakowski Grzegorz.
    Academic press:
    Pathogens (rok: 2020, tom: 9, strony: 237), Wydawca: MDPI, Basel, Switzerland
    Status:
    Published
    DOI:
    10.3390/pathogens9030237 - link to the publication
  12. Four years of African swine fever in Poland. New insights into epidemiology and prognosis of future disease spread.
    Authors:
    Pejsak Zygmunt, Niemczuk Krzysztof, Frant Maciej, Mazur Natalia, Pomorska-Mól Małgorzata, Ziętek-Barszcz Anna, Bocian Łukasz, Łyjak Magdalena, Borowska Dominika, Woźniakowski Grzegorz.
    Academic press:
    Polish Journal of Veterinary Sciences (rok: 2018, tom: 21, strony: 835-841), Wydawca: Polish Academy of Sciences Committee of Veterinary Sciences and the University of Warmia and Mazury in Olsztyn.
    Status:
    Published
    DOI:
    10.24425/pjvs.2018.125598. - link to the publication
  13. The first complete genomic sequences of the African swine fever virus isolated in Poland
    Authors:
    Natalia Mazur-Panasiuk, Grzegorz Woźniakowski, Krzysztof Niemczuk
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 4556), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41598-018-36823-0 - link to the publication
  1. ISOLATION, MOLECULAR ANALYSIS AND PREPARATION OF AFRICAN SWINE FEVER STRAINS FOR CRISPR/CAS9 MODIFICATION.
    Authors:
    Grzegorz Woźniakowski, Natalia Mazur
    Conference:
    GENOME ENGINEERING: THE CRISPR-CAS REVOLUTION (rok: 2017, ), Wydawca: Cold Spring Harbor Laboratory
    Data:
    konferencja 21-24.07.2017
    Status:
    Published

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