Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

Interactions of the potassium channel ROMK2 with mitochondrial proteins

2015/19/B/NZ1/02794

Keywords:

mitochondria potassium channels ATP

Descriptors:

  • NZ1_3: Biophysics
  • NZ1_2: Biochemistry
  • NZ1_1: Molecular biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Biologii Doświadczalnej im. M. Nenckiego Polskiej Akademii Nauk

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Piotr Koprowski 

Number of co-investigators in the project: 3

Call: OPUS 10 - announced on 2015-09-15

Amount awarded: 875 800 PLN

Project start date (Y-m-d): 2016-07-12

Project end date (Y-m-d): 2020-07-11

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. -.
  2. Inkubator CO2 do hodowli komórkowych.
  3. System do pionowej elektroforezy z chłodząco-grzejącą łaźnią cyrkulacyjną (30 000 PLN)
  4. Termomikser (25 000 PLN)

Information in the final report

  • Publication in academic press/journals (7)
  • Articles in post-conference publications (9)
  • Book publications / chapters in book publications (1)
  1. Mitochondrial potassium channels – an overview
    Authors:
    Szewczyk A, Bednarczyk P, Jędraszko J, Kampa RP, Koprowski P, Krajewska M, Kucman S, Kulawiak B, Laskowski M, Rotko D, Sęk A, Walewska A, Żochowska M, Wrzosek A.
    Academic press:
    Postepy Biochemii (rok: 2018, tom: 64(3), strony: 196-212), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
    DOI:
    10.18388/pb.2018_132 - link to the publication
  2. Solubilization, purification, and functional reconstitution of human ROMK potassium channel in copolymer styrene-maleic acid (SMA) nanodiscs
    Authors:
    Krajewska M., Koprowski P.
    Academic press:
    Biochimica et Biophysica Acta - Biomembranes (rok: 2021, tom: 1863(4), strony: -), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbamem.2021.183555 - link to the publication
  3. Interaction of ROMK2 channel with lipid kinases DGKE and AGK: potential channel activation by localized anionic lipid synthesis
    Authors:
    Milena Krajewska, Mariusz Możajew, Sławomir Filipek, Piotr Koprowski
    Academic press:
    Journal of Molecular Biology (rok: 2023, tom: brak, strony: brak), Wydawca: Elsevier
    Status:
    Submitted
    DOI:
    brak - link to the publication
  4. Mechanosensitivity of mitochondrial largeconductance calcium-activated potassium channels
    Authors:
    Agnieszka Walewska, Bogusz Kulawiak, Adam Szewczyk, Piotr Koprowski
    Academic press:
    Biochimica et Biophysica Acta (BBA) - Bioenergetics (rok: 2018, tom: 9, strony: 797-805), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbabio.2018.05.006 - link to the publication
  5. Pharmacological Characterization of a Recombinant Mitochondrial ROMK2 Potassium Channel Expressed in Bacteria and Reconstituted in Planar Lipid Bilayers
    Authors:
    Milena Krajewska, Adam Szewczyk, Bogusz Kulawiak, Piotr Koprowski
    Academic press:
    Membranes (rok: 2023, tom: 13, strony: 360), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/membranes13030360 - link to the publication
  6. Electrochemical studies of the mitochondrial ROMK2 potassium channel activity reconstituted into the free-standing and tethered bilayer lipid membranes
    Authors:
    Aleksandra Stefanowska, Piotr Koprowski, Piotr Bednarczyk, Adam Szewczyk, Pawel Krysinski
    Academic press:
    Bioelectrochemistry (rok: 2023, tom: 151, strony: 108372), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bioelechem.2023.108372 - link to the publication
  7. Gas Signaling Molecules and Mitochondrial Potassium Channels
    Authors:
    Agnieszka Walewska, Adam Szewczyk, Piotr Koprowski
    Academic press:
    International Journal of Molecular Sciences (rok: 2018, tom: 19(10), strony: 3227-3247), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms19103227 - link to the publication
  1. Characterization of ROMK2 protein activity
    Authors:
    Milena Krajewska, Piotr Bednarczyk, Piotr Koprowski, Adam Szewczyk
    Conference:
    Tools for Structural Biology of Membrane – EMBO Wookshop (rok: 2019, ), Wydawca: EMBO/EMBL
    Data:
    konferencja 43745
    Status:
    Published
  2. Regulation of activity of mitochondrial potassium channels
    Authors:
    Piotr Koprowski, Milena Krajewska, Agnieszka Walewska, Adam Szewczyk
    Conference:
    Mitoest 2018 (rok: 2018, ), Wydawca: National Institute of Chemical Physics and Biophysics
    Data:
    konferencja 43214
    Status:
    Published
  3. Synthesis of ROMK1/2 protein in Eschericha coli for functional studies
    Authors:
    Krajewska M, Koprowski P, Szewczyk A
    Conference:
    7th MITOCHONDRION 2018 "Mitochondria in the spotlight" (rok: 2018, ), Wydawca: Uniwersytet Wrocławski
    Data:
    konferencja 18-19 październik
    Status:
    Published
  4. Synthesis of Romk1/2 Protein in E.Coli
    Authors:
    Milena Krajewska, Piotr Koprowski, Adam Szewczyk
    Conference:
    63rd Annual Meeting of the Biophysical Society (rok: 2019, ), Wydawca: Biophysical Society
    Data:
    konferencja 43526
    Status:
    Published
  5. Production of ROMK1/2 protein in E. coli for functional studies
    Authors:
    Milena Krajewska, Piotr Koprowski, Adam Szewczyk
    Conference:
    20th European Bioenergetics Conference, EBEC 2018 (rok: 2018, ), Wydawca: Elsevier B. V.
    Data:
    konferencja 25-30 sierpnia
    Status:
    Published
  6. An attempt to isolate mitochondrial membrane protein complexes in membrane nanodiscs
    Authors:
    M. Krajewska, A. Kiełbasa, P. Koprowski, B. Kulawiak, A. Szewczyk
    Conference:
    42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) on From Molecules to Cells and Back (rok: 2017, ), Wydawca: FEBS Press
    Data:
    konferencja 10-14 września
    Status:
    Published
  7. Bio-ID as a method to search for partners of mitochondrial channels
    Authors:
    Krajewska M., Żochowska M., Koprowski P., Szewczyk A.
    Conference:
    6th Mitochondrion 2017 'Bioenergetics Update", 8.12.2017 (rok: 2017, ), Wydawca: Uniwersytet Adama Mickiewicza w Poznaniu
    Data:
    konferencja 08.12.2017
    Status:
    Published
  8. Recording channel activity of the ROMK1/2 protein produced in E. coli
    Authors:
    Piotr Koprowski, Milena Krajewska, Adam Szewczyk
    Conference:
    20th European Bioenergetics Conference, EBEC 2018 (rok: 2018, ), Wydawca: Elsievier B. V.
    Data:
    konferencja 25-30 sierpnia
    Status:
    Published
  9. Mitochondrial regulation of mitochondrial potassium channels
    Authors:
    Adam Szewczyk, Piotr Koprowski, Bogusz Kulawiak, Piotr Bednarczyk
    Conference:
    20th European Bioenergetics Conference, EBEC 2018 (rok: 2018, ), Wydawca: Elsevier B. V.
    Data:
    konferencja 25-30 sierpnia
    Status:
    Published
  1. Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane
    Authors:
    Bednarczyk P., Kampa RP., Kucman S., Sęk A.,Walewska A., Koprowski P.
    Book:
    Weissig V., Edeas M. (eds) Mitochondrial Medicine. Methods in Molecular Biology (rok: 2021, tom: 2276, strony: -), Wydawca: V. Weissig, M. Edeas, Editors. Humana Press, Springer New York Heidelberg Dordrecht London
    Status:
    Published

O Narodowym Centrum Nauki

Finansowanie nauki

Współpraca zagraniczna

Centrum prasowe

Deklaracja dostępności
Przetwarzanie danych osobowych
Polityka cookies

Partnerzy