Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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From selective detection of cellular oxidants and small molecule signaling agents towards better understanding of their biological chemistry

2015/18/E/ST4/00235

Keywords:

fluorescent probes peroxynitrite hydrogen peroxide superoxide nitroxyl

Descriptors:

  • ST4_3:
  • ST4_14:
  • ST4_16:

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Politechnika Łódzka, Wydział Chemiczny

woj. łódzkie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Adam Bartłomiej Sikora 

Number of co-investigators in the project: 6

Call: SONATA BIS 5 - announced on 2015-06-15

Amount awarded: 1 956 784 PLN

Project start date (Y-m-d): 2016-04-11

Project end date (Y-m-d): 2022-04-10

Project duration:: 72 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Inkubator CO2 (taki jak NU-5810E) (35 000 PLN)
  2. Pojemnik z ciekłwym azotem do przechowywania linii komórkowych (taki jak LS 3000) (20 000 PLN)
  3. Komora laminarna z recyrkulacją (taka jak Biohazard NU-480-400) (50 000 PLN)
  4. Mikroskopu odwrócony do obserwacji w technice jasnego pola (Olympus CKX41) (40 000 PLN)
  5. Wirówka wielofunkcyjna z mrożeniem (taka jak Eppendors 5430 R) (30 000 PLN)
  6. Autoklaw poziomy pojemności ok. 65 litrów (taki jak Tuttnauer 3850 EL - CPVG) (75 000 PLN)
  7. Czytnik mikropłytek (taki jak POLARstar Omega) (300 000 PLN)
  8. Chłodziarka (taka jak LR 500) (15 000 PLN)

Information in the final report

  • Publication in academic press/journals (16)
  • Book publications / chapters in book publications (2)
  1. Kinetics of Azanone (HNO) Reactions with Thiols: Effect of pH
    Authors:
    Renata Smulik-Izydorczyk, Karolina Dębowska, Michał Rostkowski, Jan Adamus, Radosław Michalski, Adam Sikora
    Academic press:
    Cell biochemistry and biophysics (rok: 2021, tom: 79, strony: 845-856), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s12013-021-00986-x - link to the publication
  2. Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases.
    Authors:
    Zielonka J, Hardy M, Michalski R, Sikora A, Zielonka M, Cheng G, Ouari O, Podsiadły R, Kalyanaraman B.
    Academic press:
    Cell Biochemistry and Biophysics (rok: 2017, tom: 75, strony: 335-349), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s12013-017-0813-6 - link to the publication
  3. Kinetic Study on the Reactivity of Azanone (HNO) toward Cyclic C-Nucleophiles
    Authors:
    Angelika Artelska, Monika Rola, Michał Rostkowski, Marlena Pięta, Jakub Pięta, Radosław Michalski, Adam Bartłomiej Sikora
    Academic press:
    International journal of molecular sciences (rok: 2021, tom: 22, strony: 12982), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms222312982 - link to the publication
  4. Decomposition of Piloty's acid derivatives - Toward the understanding of factors controlling HNO release.
    Authors:
    Smulik-Izydorczyk Renata, Rostkowski Michał, Gerbich Angelika, Jarmoc Dominika, Adamus Jan, Leszczyńska Agnieszka, Michalski Radosław, Marcinek Andrzej, Kramkowski Karol, Sikora Adam
    Academic press:
    Archives of Biochemistry and Biophysics (rok: 2019, tom: 661, strony: 132-144), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.abb.2018.11.012 - link to the publication
  5. On the chemical reactivity of tricyanofuran(TCF)-based near-infrared fluorescent redox probes – Effects of glutathione on the probe response and product fluorescence
    Authors:
    Przemysław Siarkiewicz, Radosław Michalski, Adam Sikora, Renata Smulik-Izydorczyk, Marcin Szala, Aleksandra Grzelakowska, Julia Modrzejewska, Asha Bailey, Jacek E. Nycz, Balaraman Kalyanaraman, Jan Grzegorz Malecki, Jacek Zielonka,Radosław Podsiadły
    Academic press:
    Dyes and Pigments (rok: 2021, tom: 192, strony: 109405), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.dyepig.2021.109405 - link to the publication
  6. Oxidation of ethidium-based probes by biological radicals: mechanism, kinetics and implications for the detection of superoxide
    Authors:
    Michalski R, Thiebaut D, Michałowski B, Ayhan MM, Hardy M, Ouari O, Rostkowski M, Smulik-Izydorczyk R, Artelska A, Marcinek A, Zielonka J, Kalyanaraman B, Sikora A.
    Academic press:
    Scientific Reports (rok: 2020, tom: 10, strony: 18626), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-020-75373-2 - link to the publication
  7. Fluorescent probes for the detection of nitroxyl (HNO).
    Authors:
    Smulik-Izydorczyk Renata, Dębowska Karolina, Pięta Jakub, Michalski Radosław, Marcinek Andrzej, Sikora Adam
    Academic press:
    Free Radical Biology and Medicine (rok: 2018, tom: 128, strony: 69-83), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.freeradbiomed.2018.04.564 - link to the publication
  8. Modified Metformin as a More Potent Anticancer Drug: Mitochondrial Inhibition, Redox Signaling, Antiproliferative Effects and Future EPR Studies.
    Authors:
    Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell MB.
    Academic press:
    Cell Biochemistry and Biophysics (rok: 2017, tom: 75, strony: 311-317), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s12013-017-0796-3 - link to the publication
  9. Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology
    Authors:
    Sikora A, Zielonka J, Dębowska K, Michalski R, Smulik-Izydorczyk R, Pięta J, Podsiadły R, Artelska A, Pierzchała K, Kalyanaraman B.
    Academic press:
    Frontiers in Chemistry (rok: 2020, tom: 8, strony: 580899), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fchem.2020.580899 - link to the publication
  10. Mitochondria-Targeted Triphenylphosphonium-Based Compounds: Syntheses, Mechanisms of Action, and Therapeutic and Diagnostic Applications.
    Authors:
    Zielonka J, Joseph J, Sikora A, Hardy M, Ouari O, Vasquez-Vivar J, Cheng G, Lopez M, Kalyanaraman B.
    Academic press:
    Chemical Reviews (rok: 2017, tom: 117, strony: 10043-10120), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acs.chemrev.7b00042 - link to the publication
  11. A kinetic study on the reactivity of azanone (HNO) toward its selected scavengers: Insight into its chemistry and detection.
    Authors:
    Smulik-Izydorczyk R, Mesjasz A, Gerbich A, Adamus J, Michalski R, Sikora A.
    Academic press:
    Nitric Oxide (rok: 2017, tom: 69, strony: 61-68), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.niox.2017.05.003 - link to the publication
  12. A kinetic study on the reactivity of azanone (HNO) toward its selected scavengers: Insight into its chemistry and detection.
    Authors:
    Smulik-Izydorczyk R, Mesjasz A, Gerbich A, Adamus J, Michalski R, Sikora A.
    Academic press:
    Nitric Oxide (rok: 2017, tom: 69, strony: 61-68), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.niox.2017.05.003 - link to the publication
  13. Detection and Characterization of Reactive Oxygen and Nitrogen Species in Biological Systems by Monitoring Species-Specific Products.
    Authors:
    Hardy M, Zielonka J, Karoui H, Sikora A, Michalski R, Podsiadły R, Lopez M, Vasquez-Vivar J, Kalyanaraman B, Ouari O.
    Academic press:
    Antioxidants & Redox Signaling (rok: 2018, tom: 28, strony: 1416-1432), Wydawca: Mary Ann Liebert, Inc.
    Status:
    Published
    DOI:
    10.1089/ars.2017.7398 - link to the publication
  14. The Chemistry of HNO: Mechanisms and Reaction Kinetics
    Authors:
    Radosław Michalski, Renata Smulik-Izydorczyk, Jakub Pięta, Monika Rola,Angelika Artelska, Karolina Pierzchała, Jacek Zielonka, Balaraman Kalyanaraman Adam Bartłomiej Sikora
    Academic press:
    Frontiers in Chemistry (rok: 2022, tom: 10, strony: 930657), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fchem.2022.930657 - link to the publication
  15. Decomposition of Piloty's acid derivatives - Toward the understanding of factors controlling HNO release.
    Authors:
    Smulik-Izydorczyk Renata, Rostkowski Michał, Gerbich Angelika, Jarmoc Dominika, Adamus Jan, Leszczyńska Agnieszka, Michalski Radosław, Marcinek Andrzej, Kramkowski Karol, Sikora Adam
    Academic press:
    Archives of Biochemistry and Biophysics (rok: 2019, tom: 661, strony: 132-144), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.abb.2018.11.012 - link to the publication
  16. Mitochondria-targeted metformins: anti-tumour and redox signalling mechanisms.
    Authors:
    Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell M.
    Academic press:
    Interface Focus (rok: 2017, tom: 7, strony: 20160109), Wydawca: The Royal Society
    Status:
    Published
    DOI:
    10.1098/rsfs.2016.0109 - link to the publication
  1. Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid
    Authors:
    Zielonka J, Sikora A, Podsiadly R, Hardy M, Kalyanaraman B
    Book:
    Methods in Molecular Biology "Mitochondrial Medicine" (rok: 2021, tom: 2275, strony: 315-327), Wydawca: Springer
    Status:
    Published
  2. Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid
    Authors:
    Zielonka J, Sikora A, Podsiadly R, Hardy M, Kalyanaraman B
    Book:
    Methods in Molecular Biology "Mitochondrial Medicine" (rok: 2021, tom: 2275, strony: 315-327), Wydawca: Springer
    Status:
    Published

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