Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

Comperative analysis of the developmental stages of Trichinella britovi using proteomic tools and indication of parasite proteins with immunodiagnostic potential

2015/18/E/NZ6/00502

Keywords:

Trichinella britovi antigens 2D DIGE Western Blot immunogenicity

Descriptors:

  • NZ6_6: Parasitology

Panel:

NZ6 - Human and animal immunology and infection: immunity, immune disorders, immunotherapy, infectious and invasive diseases, microbiology, transplantology, allergology

Host institution :

Instytut Parazytologii im. Witolda Stefańskiego PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Justyna Bień-Kalinowska 

Number of co-investigators in the project: 5

Call: SONATA BIS 5 - announced on 2015-06-15

Amount awarded: 1 240 800 PLN

Project start date (Y-m-d): 2016-04-21

Project end date (Y-m-d): 2020-04-20

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Aparat do pionowej elektroforezy i western blottingu (6 000 PLN)
  2. Komora laminarna (48 000 PLN)
  3. Urzadzenie do dokumentacji, wizualizacji, zapisu i analizy próbek znakowanych fluorescencyjnie, chemiluminescencyjnie oraz kolorymetrycznie. (270 000 PLN)

Information in the final report

  • Publication in academic press/journals (6)
  • Articles in post-conference publications (8)
  1. Trichinella britovi muscle larvae and adult worms: stage-specific and common antigens detected by two-dimensional gel electrophoresis-based immunoblotting
    Authors:
    Sylwia Grzelak, Bożena Moskwa,Justyna Bień
    Academic press:
    Parasites and Vectors (rok: 2018, tom: 11(1):584, strony: 17), Wydawca: Bio Med Central (part of Springer Nature)
    Status:
    Published
    DOI:
    10.1186/s13071-018-3177-x - link to the publication
  2. Exploiting the potential of 2D DIGE and 2DE immunoblotting for comparative analysis of crude extract of Trichinella britovi and Trichinella spiralis muscle larvae proteomes
    Authors:
    Sylwia Grzelak, Anna Stachyra, Bożena Moskwa , Justyna Bień-Kalinowska
    Academic press:
    Veterinary Parasitology (rok: 2021, tom: 289:109323., strony: 15), Wydawca: ELSEVIER
    Status:
    Published
    DOI:
    10.1016/j.vetpar.2020.109323. - link to the publication
  3. Immunization with a Recombinant Protein of Trichinella britovi 14-3-3 Triggers an Immune Response but No Protection in Mice
    Authors:
    Anna Stachyra, Sylwia Grzelak, Katarzyna Basałaj, Anna Zawistowska-Deniziak , Justyna Bień-Kalinowska
    Academic press:
    Vaccines (Basel) (rok: 2020, tom: 8(3):515, strony: 14), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/vaccines8030515. - link to the publication
  4. Immunoproteomic analysis of Trichinella spiralis and Trichinella britovi excretory-secretory muscle larvae proteins recognized by sera from humans infected with Trichinella
    Authors:
    Sylwia Grzelak , Anna Stachyra, Jerzy Stefaniak, Karolina Mrówka, Bożena Moskwa, Justyna Bień-Kalinowska
    Academic press:
    Plos ONE (rok: 2020, tom: 15(11):e0241918, strony: 19), Wydawca: Plos One PMC
    Status:
    Published
    DOI:
    10.1371/journal.pone.0241918 - link to the publication
  5. The first analysis of Trichinella spiralis and Trichinella britovi adult worm excretory-secretory proteins by twodimensional electrophoresis coupled with LC-MS/MS
    Authors:
    Sylwia Grzelak, Anna Stachyra, Justyna BieńKalinowska
    Academic press:
    Veterinary Parasitology (rok: 2021, tom: 109096, strony: 7), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.vetpar.2020.109096 - link to the publication
  6. The Immunological Properties of Recombinant Multi-Cystatin-Like Domain Protein From Trichinella Britovi Produced in Yeast.
    Authors:
    Stachyra A, ZawistowskaDeniziak A, Basałaj K, Grzelak S, Gondek M, Bień-Kalinowska J.
    Academic press:
    Frontiers in Immunology (rok: 2019, tom: 10,242, strony: 11), Wydawca: Frontiers Media S.A
    Status:
    Published
    DOI:
    10.3389/fim - link to the publication
  1. Cloning, expression and identification of Trichinella britovi 14-3-3-like protein 2 (14- 3-3-LP2)
    Authors:
    Anna Stachyra, Anna Zawistowska-Deniziak, Katarzyna Basałaj, Justyna Bień
    Conference:
    14th International Congress of Parasitology, Exco, Daegu, Korea, (rok: 2018, ), Wydawca: ICOPA 2018
    Data:
    konferencja 19-24.08.2018
    Status:
    Published
  2. Cloning, expression and identification of Trichinella britovi multi-cystatin-like domain protein (CLP)
    Authors:
    Anna Stachyra, Katarzyna Basałaj, Anna Zawistowska-Deniziak, Justyna Bień
    Conference:
    14th International Congress of Parasitology, Exco, Daegu, Korea, (rok: 2018, ), Wydawca: ICOPA 2018
    Data:
    konferencja 19-24.08.2018
    Status:
    Published
  3. Immuno-proteomic analysis of Trichinella spiralis and Trichinella britovi excretory-secretory antigens recognized by human Trichinella-infected sera
    Authors:
    Justyna Bień-Kalinowska, Sylwia Grzelak, Anna Stachyra, Jerzy Stefaniak, Bożena Moskwa
    Conference:
    The XXV Congress of the Polish Parasitological Society, 9-12 September 2019, Warsaw (rok: 2019, ), Wydawca: Annals of Parasitology, Polish Parasitological Society
    Data:
    konferencja 9-12 September 2019
    Status:
    Published
  4. Immunogenicity of recombinant multi-cystatin-like domain protein from T. britovi: In Vivo study in mouse model.
    Authors:
    Anna Stachyra, Katarzyna Basałaj, Anna ZawistowskaDeniziak, Sylwia Grzelak, Justyna Bień-Kalinowska
    Conference:
    15th International Conference on Trichinellosis (rok: 2019, ), Wydawca: Fundatia Scientia Parasitologica Pro Vita
    Data:
    konferencja 26-30 August 2019, Cluj-Napoca, Romania
    Status:
    Published
  5. Comparative analysis of excretorysecretory antigens of Trichinella spiralis and Trichinella britovi adult worm by two-dimensional gel electrophoresis coupled with immunoblotting.
    Authors:
    Sylwia Grzelak, Justyna Bień-Kalinowska
    Conference:
    15th International Conference on Trichinellosis (rok: 2019, ), Wydawca: Fundatia Scientia Parasitologica Pro Vita
    Data:
    konferencja 26-30 August 2019, Cluj Napoca, Romania
    Status:
    Published
  6. Immunogenicity studies of recombinant multi-cystatin-like domain protein from Trichinella britovi produced in yeast
    Authors:
    Anna Stachyra, Katarzyna Basałaj, Anna ZawistowskaDeniziak, Sylwia Grzelak, Justyna Bień-Kalinowska
    Conference:
    The XXV Congress of the Polish Parasitological Society, 9-12 September 2019, Warsaw (rok: 2019, ), Wydawca: Annals of Parasitology, Polish Parasitological Society
    Data:
    konferencja 9-12 September 2019, Warsaw
    Status:
    Published
  7. Proteomic analysis and immnunodetection of antigens from early developmental stages of Trichinella britov
    Authors:
    Sylwia Grzelak, Anna Stachyra, Justyna Bień-Kalinowska
    Conference:
    The XXV Congress of the Polish Parasitological Society, 9-12 September 2019, Warsaw (rok: 2019, ), Wydawca: Annals of Parasitology, Polish Parasitological Society
    Data:
    konferencja 9-12 September 2019, Warsaw
    Status:
    Published
  8. Trichinella britovi muscle larvae and adult worms: Stage-specific and common antigens detected by two dimensional gel electrophoresis (2-DE) based immunoblotting
    Authors:
    Grzelak Sylwia, Bień Justyna
    Conference:
    14th International Congress of Parasitology, Exco, Daegu, Korea, (rok: 2018, ), Wydawca: ICOPA 2018
    Data:
    konferencja 19-24.08.2018
    Status:
    Published

O Narodowym Centrum Nauki

Finansowanie nauki

Współpraca zagraniczna

Centrum prasowe

Deklaracja dostępności
Przetwarzanie danych osobowych
Polityka cookies

Partnerzy