Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Comperative analysis of the developmental stages of Trichinella britovi using proteomic tools and indication of parasite proteins with immunodiagnostic potential

2015/18/E/NZ6/00502

Keywords:

Trichinella britovi antigens 2D DIGE Western Blot immunogenicity

Descriptors:

  • NZ6_6: Parasitology

Panel:

NZ6 - Human and animal immunology and infection: immunity, immune disorders, immunotherapy, infectious and invasive diseases, microbiology, transplantology, allergology

Host institution :

Instytut Parazytologii im. Witolda Stefańskiego PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Justyna Bień-Kalinowska 

Number of co-investigators in the project: 5

Call: SONATA BIS 5 - announced on 2015-06-15

Amount awarded: 1 240 800 PLN

Project start date (Y-m-d): 2016-04-21

Project end date (Y-m-d): 2020-04-20

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Aparat do pionowej elektroforezy i western blottingu (6 000 PLN)
  2. Komora laminarna (48 000 PLN)
  3. Urzadzenie do dokumentacji, wizualizacji, zapisu i analizy próbek znakowanych fluorescencyjnie, chemiluminescencyjnie oraz kolorymetrycznie. (270 000 PLN)

Information in the final report

  • Publication in academic press/journals (6)
  • Articles in post-conference publications (8)
  1. Exploiting the potential of 2D DIGE and 2DE immunoblotting for comparative analysis of crude extract of Trichinella britovi and Trichinella spiralis muscle larvae proteomes
    Authors:
    Sylwia Grzelak, Anna Stachyra, Bożena Moskwa , Justyna Bień-Kalinowska
    Academic press:
    Veterinary Parasitology (rok: 2021, tom: 289:109323., strony: 15), Wydawca: ELSEVIER
    Status:
    Published
    DOI:
    10.1016/j.vetpar.2020.109323. - link to the publication
  2. Immunoproteomic analysis of Trichinella spiralis and Trichinella britovi excretory-secretory muscle larvae proteins recognized by sera from humans infected with Trichinella
    Authors:
    Sylwia Grzelak , Anna Stachyra, Jerzy Stefaniak, Karolina Mrówka, Bożena Moskwa, Justyna Bień-Kalinowska
    Academic press:
    Plos ONE (rok: 2020, tom: 15(11):e0241918, strony: 19), Wydawca: Plos One PMC
    Status:
    Published
    DOI:
    10.1371/journal.pone.0241918 - link to the publication
  3. The first analysis of Trichinella spiralis and Trichinella britovi adult worm excretory-secretory proteins by twodimensional electrophoresis coupled with LC-MS/MS
    Authors:
    Sylwia Grzelak, Anna Stachyra, Justyna BieńKalinowska
    Academic press:
    Veterinary Parasitology (rok: 2021, tom: 109096, strony: 7), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.vetpar.2020.109096 - link to the publication
  4. Trichinella britovi muscle larvae and adult worms: stage-specific and common antigens detected by two-dimensional gel electrophoresis-based immunoblotting
    Authors:
    Sylwia Grzelak, Bożena Moskwa,Justyna Bień
    Academic press:
    Parasites and Vectors (rok: 2018, tom: 11(1):584, strony: 17), Wydawca: Bio Med Central (part of Springer Nature)
    Status:
    Published
    DOI:
    10.1186/s13071-018-3177-x - link to the publication
  5. The Immunological Properties of Recombinant Multi-Cystatin-Like Domain Protein From Trichinella Britovi Produced in Yeast.
    Authors:
    Stachyra A, ZawistowskaDeniziak A, Basałaj K, Grzelak S, Gondek M, Bień-Kalinowska J.
    Academic press:
    Frontiers in Immunology (rok: 2019, tom: 10,242, strony: 11), Wydawca: Frontiers Media S.A
    Status:
    Published
    DOI:
    10.3389/fim - link to the publication
  6. Immunization with a Recombinant Protein of Trichinella britovi 14-3-3 Triggers an Immune Response but No Protection in Mice
    Authors:
    Anna Stachyra, Sylwia Grzelak, Katarzyna Basałaj, Anna Zawistowska-Deniziak , Justyna Bień-Kalinowska
    Academic press:
    Vaccines (Basel) (rok: 2020, tom: 8(3):515, strony: 14), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/vaccines8030515. - link to the publication
  1. Immunogenicity studies of recombinant multi-cystatin-like domain protein from Trichinella britovi produced in yeast
    Authors:
    Anna Stachyra, Katarzyna Basałaj, Anna ZawistowskaDeniziak, Sylwia Grzelak, Justyna Bień-Kalinowska
    Conference:
    The XXV Congress of the Polish Parasitological Society, 9-12 September 2019, Warsaw (rok: 2019, ), Wydawca: Annals of Parasitology, Polish Parasitological Society
    Data:
    konferencja 9-12 September 2019, Warsaw
    Status:
    Published
  2. Immuno-proteomic analysis of Trichinella spiralis and Trichinella britovi excretory-secretory antigens recognized by human Trichinella-infected sera
    Authors:
    Justyna Bień-Kalinowska, Sylwia Grzelak, Anna Stachyra, Jerzy Stefaniak, Bożena Moskwa
    Conference:
    The XXV Congress of the Polish Parasitological Society, 9-12 September 2019, Warsaw (rok: 2019, ), Wydawca: Annals of Parasitology, Polish Parasitological Society
    Data:
    konferencja 9-12 September 2019
    Status:
    Published
  3. Trichinella britovi muscle larvae and adult worms: Stage-specific and common antigens detected by two dimensional gel electrophoresis (2-DE) based immunoblotting
    Authors:
    Grzelak Sylwia, Bień Justyna
    Conference:
    14th International Congress of Parasitology, Exco, Daegu, Korea, (rok: 2018, ), Wydawca: ICOPA 2018
    Data:
    konferencja 19-24.08.2018
    Status:
    Published
  4. Cloning, expression and identification of Trichinella britovi 14-3-3-like protein 2 (14- 3-3-LP2)
    Authors:
    Anna Stachyra, Anna Zawistowska-Deniziak, Katarzyna Basałaj, Justyna Bień
    Conference:
    14th International Congress of Parasitology, Exco, Daegu, Korea, (rok: 2018, ), Wydawca: ICOPA 2018
    Data:
    konferencja 19-24.08.2018
    Status:
    Published
  5. Cloning, expression and identification of Trichinella britovi multi-cystatin-like domain protein (CLP)
    Authors:
    Anna Stachyra, Katarzyna Basałaj, Anna Zawistowska-Deniziak, Justyna Bień
    Conference:
    14th International Congress of Parasitology, Exco, Daegu, Korea, (rok: 2018, ), Wydawca: ICOPA 2018
    Data:
    konferencja 19-24.08.2018
    Status:
    Published
  6. Immunogenicity of recombinant multi-cystatin-like domain protein from T. britovi: In Vivo study in mouse model.
    Authors:
    Anna Stachyra, Katarzyna Basałaj, Anna ZawistowskaDeniziak, Sylwia Grzelak, Justyna Bień-Kalinowska
    Conference:
    15th International Conference on Trichinellosis (rok: 2019, ), Wydawca: Fundatia Scientia Parasitologica Pro Vita
    Data:
    konferencja 26-30 August 2019, Cluj-Napoca, Romania
    Status:
    Published
  7. Comparative analysis of excretorysecretory antigens of Trichinella spiralis and Trichinella britovi adult worm by two-dimensional gel electrophoresis coupled with immunoblotting.
    Authors:
    Sylwia Grzelak, Justyna Bień-Kalinowska
    Conference:
    15th International Conference on Trichinellosis (rok: 2019, ), Wydawca: Fundatia Scientia Parasitologica Pro Vita
    Data:
    konferencja 26-30 August 2019, Cluj Napoca, Romania
    Status:
    Published
  8. Proteomic analysis and immnunodetection of antigens from early developmental stages of Trichinella britov
    Authors:
    Sylwia Grzelak, Anna Stachyra, Justyna Bień-Kalinowska
    Conference:
    The XXV Congress of the Polish Parasitological Society, 9-12 September 2019, Warsaw (rok: 2019, ), Wydawca: Annals of Parasitology, Polish Parasitological Society
    Data:
    konferencja 9-12 September 2019, Warsaw
    Status:
    Published

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