Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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"Role of mobile genetic elements in bacteria metabolism. Dynamic alpha-helical polymers of Kfr-type proteins in organization of prokaryotic mitotic spindle".

2015/17/B/NZ2/01160

Keywords:

alpha-helical proteins self-assembly DNA segregation plasmid stability

Descriptors:

  • NZ2_4: Metabolomics
  • NZ2_9: Systems biology
  • NZ2_12: Other related subjects

Panel:

NZ2 - Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology

Host institution :

Politechnika Warszawska, Wydział Chemiczny

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Małgorzata Adamczyk 

Number of co-investigators in the project: 10

Call: OPUS 9 - announced on 2015-03-16

Amount awarded: 1 384 200 PLN

Project start date (Y-m-d): 2016-05-06

Project end date (Y-m-d): 2020-11-05

Project duration:: 54 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Termostatowana wytrzasarka firmy Eppendorf na różne typy probówek (18 000 PLN)
  2. Urządzenie wielofunkcyjne.
  3. drukarka (1 500 PLN)
  4. komputer przenośny typu laptop (4 000 PLN)
  5. pipety automatyczne i elektroniczne (10 000 PLN)
  6. skaner biurowy (500 PLN)
  7. Zestaw horyzontalny do elektroforezy DNA z zasilaczem (12 000 PLN)
  8. personal komputer 2TB (7 000 PLN)
  9. Termomixer z wymiennym blokiem grzewczym.
  10. mikrowirówka stołowa (4 500 PLN)
  11. Zestaw do elektroforezy DNA (2 szt.) (7 000 PLN)
  12. zestaw do elektroforezy białkowej (10 500 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  • Articles in post-conference publications (2)
  1. Alpha-Helical Protein KfrC Acts as a Switch between the Lateral and Vertical Modes of Dissemination of Broad-Host-Range RA3 Plasmid from IncU (IncP-6) Incompatibility Group
    Authors:
    Monika Mitura, Ewa Lewicka, Jolanta Godziszewska, Malgorzata Adamczyk, Grazyna Jagura-Burdzy
    Academic press:
    Int J Mol Sci (rok: 2021, tom: 22(9), strony: 4880), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms22094880 - link to the publication
  2. Transcriptional organization of the stability module of Broad-Host-Range plasmid RA3, from the IncU group
    Authors:
    Lewicka E, Dolowy P, Godziszewska J, Litwin E, Ludwiczak M, Jagura-Burdzy G
    Academic press:
    Applied and Enviromental Microbiology (rok: 2020, tom: 86, strony: e00847-20), Wydawca: American Society for Microbiology
    Status:
    Published
    DOI:
    10.1128/AEM.00847-20 - link to the publication
  3. Unique properties of alpha-helical DNA-binding KfrA protein of RA3 plasmid from IncU incompatibility group and its host-dependent role in plasmid maintenance
    Authors:
    Lewicak E, Mitura M, Steczkiewicz K, Kieracinska J, Skrzynska K, Adamczyk M, Jagura-Burdzy G
    Academic press:
    Applied and Enviromental Microbiology (rok: 2021, tom: 87(2), strony: e01771-20), Wydawca: American Society for Microbiology
    Status:
    Published
    DOI:
    10.1128/AEM.01771-20 - link to the publication
  4. Revealing biophysical properties of KfrA-type proteins as a novel class of cytoskeletal, coiled-coil plasmid-encoded proteins
    Authors:
    Malgorzata Adamczyk, Ewa Lewicka, Roza Szatkowska, Hanna Nieznanska, Jan Ludwiczak, Ewa Sitkiewicz, Stanislaw Dunin-Horkawicz, Maciej Jasinski, Bianka Swiderska, Grazyna Goch, Grazyna Jagura-Burdzy
    Academic press:
    BMC Microbiology (rok: 2021, tom: 21(1):32, strony: e), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1186/s12866-020-02079-w. - link to the publication
  1. Revealing dynamic alpha-helical polymers of KfrAR751 plasmidic protein and mapping of its DNA-binding domain via biophysical measurements
    Authors:
    M. Kołodziejczyk, R. Szatkowska, T. Kobiela, H. Nieznanska, M. Adamczyk
    Conference:
    Talking moelecules: the networks that shape the living world (rok: 2018, ), Wydawca: COST action CA 15126 ARBRE MOBIEU Between Atom and Cell
    Data:
    konferencja 19-21 Marca
    Status:
    Published
  2. Revealing properties of the alpha-helical KfrA type proteins via biophysical measurements
    Authors:
    Kobiela T, Kołodziejczyk M, Nieznańska H, Adamczyk M
    Conference:
    Building Bridges in Biophysics (rok: 2017, ), Wydawca: COST action CA 15126 ARBRE MOBIEU Between Atom and Cell
    Data:
    konferencja 22-24 March, Porto, Portugal
    Status:
    Published

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