Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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New mechanisms of CRISPR/Cas-based site-directed mutagenesis as a tool in functional analysis of genes and genome modification in barley.

2015/17/D/NZ9/02020

Keywords:

CRISPR/Cas genome editing mutagenesis transgenesis barley Arabidopsis

Descriptors:

  • NZ9_1: Agronomy
  • NZ2_2: Genomics, transcriptomics, epigenomics
  • NZ1_9: Biotechnology

Panel:

NZ9 - Fundamentals of applied life sciences and biotechnology: agricultural, forestry, horticulture, animal production and fishery, food and nutrition sciences, industrial biosciences, environmental biotechnology and remediation

Host institution :

Instytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Sebastian Gasparis 

Number of co-investigators in the project: 3

Call: SONATA 9 - announced on 2015-03-16

Amount awarded: 515 080 PLN

Project start date (Y-m-d): 2016-01-25

Project end date (Y-m-d): 2019-08-24

Project duration:: 43 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Wirówka próżniowa z podgrzewaniem i pompą podciśnieniową (36 000 PLN)
  2. Inkubator mikrobiologiczny.
  3. Inkubator mikrobiologiczny (12 000 PLN)
  4. Zestaw do filtracji z pompą prózniową (10 000 PLN)
  5. Komora z laminarnym przepływem powietrza (30 000 PLN)

Information in the final report

  • Publication in academic press/journals (2)
  • Book publications / chapters in book publications (1)
  1. A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)
    Authors:
    Sebastian Gasparis, Maciej Kała, Mateusz Przyborowski, Leszek A. Łyznik, Wacław Orczyk, Anna Nadolska-Orczyk
    Academic press:
    Plant Methods (rok: 2018, tom: 14, strony: 45305), Wydawca: BioMed Central
    Status:
    Published
    DOI:
    10.1186/s13007-018-0382-8 - link to the publication
  2. Knockout of the HvCKX1 or HvCKX3 Gene in Barley (Hordeum vulgare L.) by RNA-Guided Cas9 Nuclease Affects the Regulation of Cytokinin Metabolism and Root Morphology
    Authors:
    Sebastian Gasparis, Mateusz Przyborowski, Maciej Kała, Anna Nadolska-Orczyk
    Academic press:
    Cells (rok: 2019, tom: 8, strony: 45315), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/cells8080782 - link to the publication
  1. An optimized RNA-guided Cas9 system for efficient simplex and multiplex genome editing in barley (Hordeum vulgare L.)
    Authors:
    Sebastian Gasparis, Mateusz Przyborowski
    Book:
    CRISPR-Cas Methods (rok: 2020, tom: 1, strony: 117-142), Wydawca: Springer US
    Status:
    Published

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