Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Conformational transition pathways and molecular mechanisms of aggregation of the amyloidogenic H-fragment of insulin

2015/17/B/NZ1/00832

Keywords:

amyloid proteolysis insulin conformational transitions spectroscopy molecular dynamics

Descriptors:

  • NZ1_3: Biophysics
  • NZ1_4: Structural biology
  • ST4_13: Solid-state chemistry, surface physicochemistry

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Warszawski, Wydział Chemii

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Wojciech Dzwolak 

Number of co-investigators in the project: 9

Call: OPUS 9 - announced on 2015-03-16

Amount awarded: 1 386 000 PLN

Project start date (Y-m-d): 2016-02-18

Project end date (Y-m-d): 2020-01-17

Project duration:: 47 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Czytnik płytkowy fluorescencji z funkcją pomiarów anizotropii fluorescencji oraz dozowania reagentów wraz z dedykowanym stanowiskiem do procesowania danych. (290 000 PLN)
  2. Aparat do produkcji ultraczystej wody (16 000 PLN)
  3. Liofilizator laboratoryjny.
  4. Zestaw do elektroforezy białek.

Information in the final report

  • Publication in academic press/journals (7)
  1. Beyond amino acid sequence: disulfide bonds and the origins of the extreme amyloidogenic properties of insulin's H-fragment
    Authors:
    Robert Dec R, Michał Koliński M, Wojciech Dzwolak
    Academic press:
    The FEBS Journal (rok: 2019, tom: 286, strony: 3194-3205), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1111/febs.14849 - link to the publication
  2. Docking interactions determine early cleavage events in insulin proteolysis by pepsin: Experiment and simulation
    Authors:
    Michał Koliński, Sebastian Kmiecik, Robert Dec, Marcin Piejko, Paweł Mak, Wojciech Dzwolak
    Academic press:
    International Journal of Biological Macromolecules (rok: 2020, tom: 149, strony: 1151-1160), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ijbiomac.2020.01.253 - link to the publication
  3. Multiscale modeling of amyloid fibril formed by aggregating peptide derived from amyloidogenic fragment of A-chain of insulin
    Authors:
    Michał Koliński, Robert Dec, Wojciech Dzwolak
    Academic press:
    International Journal of Molecular Sciences (rok: 2021, tom: 22, strony: 12325), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms222212325 - link to the publication
  4. A tale of two tails: Self-assembling properties of A- and B-chain parts of insulin's highly amyloidogenic H-fragment
    Authors:
    Robert Dec, Wojciech Dzwolak
    Academic press:
    International Journal of Biological Macromolecules (rok: 2021, tom: 186, strony: 510–518), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ijbiomac.2021.07.057 - link to the publication
  5. Extremely Amyloidogenic Single-Chain Analogues of Insulin's H-Fragment: Structural Adaptability of an Amyloid Stretch
    Authors:
    Robert Dec, Wojciech Dzwolak
    Academic press:
    Langmuir (rok: 2020, tom: 36, strony: 12150-12159), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.langmuir.0c01747 - link to the publication
  6. Reduction of a disulfide-constrained oligo-glutamate peptide triggers self-assembly of β2-type amyloid fibrils with the chiroptical properties determined by supramolecular chirality
    Authors:
    Robert Dec, Marcin Guza, Wojciech Dzwolak
    Academic press:
    International Journal of Biological Macromolecules (rok: 2020, tom: 162, strony: 866–872), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ijbiomac.2020.06.221 - link to the publication
  7. Rapid self-association of highly amyloidogenic H-fragments of insulin: Experiment and molecular dynamics simulations
    Authors:
    Robert Dec, Michał Koliński, Maksim Kouza, Wojciech Dzwolak
    Academic press:
    International Journal of Biological Macromolecules (rok: 2020, tom: 150, strony: 894-903), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ijbiomac.2020.02.153 - link to the publication

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