The role of antimicrobial protein-chemerin in skin pathophysiology.

2014/12/W/NZ6/00454

Keywords

Descriptors

  • NZ6_4: Bakteriologia
  • NZ4_4: Patofizjologia ogólna

Panel

NZ6 – Human and animal immunology and infection: immunity, immune disorders, immunotherapy, infectious and invasive diseases, microbiology, transplantology, allergology

Host institution

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

Voivodeship: małopolskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Joanna Cichy

Number of co-investigators in the project: 18

Call

SYMFONIA 2 – announced on 2013-12-16

Amount awarded
6 589 560 PLN
Project start date
2014-11-21
Project end date
2021-02-20
Project duration
75 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Aparat do kawitacji.
  2. Komputer stacjonarny. Cost: 3 676 PLN
  3. Komputer stacjonarny.
  4. Mikroskop biologiczny z kamerą cyfrową. Cost: 16 728 PLN
  5. Platforma PCR do analiz w czasie rzeczywistym.. Cost: 180 000 PLN
  6. Pompa strzykawkowe i robot do nanoszenia próbek.. Cost: 60 000 PLN
  7. Przyrząd do mierzenia wilgotości skóry.. Cost: 75 000 PLN
  8. Rozbudowa stacji roboczej (hardware i software).
  9. Stacja robocza i podajnik płytek do cytometru przepływowego LSRII.. Cost: 140 000 PLN
  10. Stacja robocza i rozbudowa cytometru o nowe lasery.
  11. System do analizy obrazów.. Cost: 170 000 PLN
  12. System do detekcji produktów PCR w czasie rzeczywistym.. Cost: 120 000 PLN
  13. Wielodetekcyjny czytnik mikropłytkowy. Cost: 100 000 PLN
  14. Zamrażarka. Cost: 849 PLN
  15. dwustanowiskowa komora laminarna.
  16. komputer stacjonarny - 2 szt..
  17. komputer stacjonarny z monitorem LCD.
  18. Łaźnia wodna. Cost: 5 227 PLN

Information in the final report

Publication in academic press/journals (32)

  1. Dodecylresorufin (C12R) outperforms resorufin in micro-droplet bacterial assays

    Authors:
    O. Scheler, T.S. Kaminski, A. Ruszczak and P. Garstecki
    Academic press:
    Applied Materials and Interfaces (year: 2016, volume: 8, pages: 11318-11325), Publisher: AMER CHEMICAL SOC
    Status:
    Published
    DOI:
    10.1021/acsami.6b02360 – link to the publication
  2. Droplet microfluidics for microbiology: techniques, applications and challenges

    Authors:
    T.S. Kaminski, O. Scheler and P. Garstecki
    Academic press:
    Lab on a Chip (year: 2016, volume: 16, pages: 2168-2187), Publisher: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C6LC00367B – link to the publication
  3. The expression and regulation of chemerin in the epidermis.

    Authors:
    Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel BA, Cichy J.
    Academic press:
    PLos One (year: 2015, volume: 10(2):e011783, pages: 46041), Publisher: Plos.org
    Status:
    Published
    DOI:
    10.1371/journal.pone.0117830 – link to the publication
  4. The methylation status of the chemerin promoter region located from - 252 to + 258 bp regulates constitutive but not acute-phase cytokine-inducible chemerin expression levels

    Authors:
    Kwiecien K, Brzoza P, Bak M, Majewski P, Skulimowska I, Bednarczyk K, Cichy J, Kwitniewski M.
    Academic press:
    Scientific Reports (year: 2020, volume: 10, pages: 13702 (1-12)), Publisher: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-020-70625-7 – link to the publication
  5. Bacteria Modify Their Sensitivity to Chemerin-Derived Peptides by Hindering Peptide Association With the Cell Surface and Peptide Oxidation

    Authors:
    Godlewska U, Bilska B, Majewski P, Pyza E, Zabel BA, Cichy J.
    Academic press:
    Frontiers in Microbiology (year: 2020, volume: 11, pages: 1819 (1-11)), Publisher: Frontiers
    Status:
    Published
    DOI:
    10.3389/fmicb.2020.01819 – link to the publication
  6. Expression of antimicrobial peptide genes oscillates along day/night rhythm protecting mice skin from bacteria

    Authors:
    Bilska B, Zegar A, Slominski AT, Kleszczyński K, Cichy J, Pyza E.
    Academic press:
    Experimental Dermatology (year: 2020, volume: 0, pages: 46032), Publisher: John Wiley & Sons Ltd
    Status:
    Published
    DOI:
    10.1111/exd.14229 – link to the publication
  7. Metagenomic Studies in Inflammatory Skin Diseases

    Authors:
    Godlewska U, Brzoza P, Kwiecień K, Kwitniewski M, Cichy J.
    Academic press:
    Current Microbiology (year: 2020, volume: 77, pages: 3201–3212), Publisher: Springer nature
    Status:
    Published
    DOI:
    10.1007/s00284-020-02163-4 – link to the publication
  8. Direct droplet digital PCR (dddPCR) for species specific, accurate and precise quantification of bacteria in mixed samples

    Authors:
    Natalia Pacocha, Ott Scheler, Mikołaj Marcin Nowak, Ladislav Derzsi, Joanna Cichy, Piotr Garstecki
    Academic press:
    Analytical Methods (year: 2019, volume: 11, pages: 5730-5735), Publisher: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C9AY01874C – link to the publication
  9. Non-wetting droplets in capillaries of circular cross-section: scaling function

    Authors:
    Karol Makuch, Jean-Baptiste Gorce, and Piotr Garstecki
    Academic press:
    Physics of Fluids (year: 2019, volume: 31, pages: 43102), Publisher: AIP Publishing
    Status:
    Published
    DOI:
    10.1063/1.5087722 – link to the publication
  10. The role of chemerin and its antibacterial derivatives in maintaining epithelial barrier function

    Authors:
    Godlewska U, Zegar A, Cichy J.
    Academic press:
    Postepy Biochemii (year: 2020, volume: 66 (2), pages: 151-159), Publisher: Polskie Towarzystwo Biochemiczne
    Status:
    Published
    DOI:
    10.18388/pb.2020_323 – link to the publication
  11. Architecture of antimicrobial skin defense.

    Authors:
    Kwiecien K, Zegar A, Jung J, Brzoza P, Kwitniewski M, Godlewska U, Grygier B, Kwiecinska P, Morytko A, Cichy J.
    Academic press:
    Cytokine & Growth Factor Reviews (year: 2019, volume: 49, pages: 70-84), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.cytogfr.2019.08.001 – link to the publication
  12. Redox Active Antimicrobial Peptides in Controlling Growth of Microorganisms at Body Barriers

    Authors:
    Brzoza, P.; Godlewska, U.; Borek, A.; Morytko, A.; Zegar, A.; Kwiecinska, P.; Zabel, B.A.; Osyczka, A.; Kwitniewski, M.; Cichy, J.
    Academic press:
    Antioxidants (year: 2021, volume: 10(3), pages: 446 (1-18)), Publisher: MDPI
    Status:
    Published
    DOI:
    link to the publication
  13. High-Throughput Monitoring of Bacterial Cell Density in Nanoliter Droplets: Label-Free Detection of Unmodified Gram-Positive and Gram-Negative Bacteria

    Authors:
    Natalia Pacocha, Jakub Bogusławski, Michał Horka, Karol Makuch,Kamil Liżewski, Maciej Wojtkowski, Piotr Garsteck
    Academic press:
    Analytical Chemistr (year: 2021, volume: 93, 2, pages: 843–850), Publisher: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.analchem.0c03408 – link to the publication
  14. Antimicrobial Properties of a Peptide Derived from the Male Fertility Factor kl2 Protein of Drosophila melanogaster

    Authors:
    Bilska, B.; Godlewska, U.; Damulewicz, M.; Murzyn, K.; Kwitniewski, M.; Cichy, J.; Pyza, E.
    Academic press:
    Current Issues in Molecular Biology (year: 2022, volume: 44, pages: 1169-1181), Publisher: MDPI
    Status:
    Published
    DOI:
    10.3390/cimb44030076 – link to the publication
  15. Chemerin-derived peptide Val66-Pro85 is effective in limiting methicillin-resistant S. aureus skin infection.

    Authors:
    Aneta Zegar, Urszula Godlewska, Dorota Kozłowska-Chmielewska, Pawel Majewski, Brian A Zabel, Joanna Cichy
    Academic press:
    Frontiers in Microbiology (year: 2021, volume: 12, pages: 46030), Publisher: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fmicb.2021.742610 – link to the publication
  16. Droplet-based digital antibiotic susceptibility screen reveals single-cell clonal heteroresistance in an isogenic bacterial population

    Authors:
    Ott Scheler, Karol Makuch, Pawel R Debski, Michal Horka, Artur Ruszczak, Natalia Pacocha, Krzysztof Sozanski, Olli-Pekka Smolander, Witold Postek, Piotr Garstecki
    Academic press:
    Scientific Reports (year: 2020, volume: 10, pages: 3282), Publisher: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41598-020-60381-z – link to the publication
  17. Gravity-driven microfluidic assay for digital enumeration of bacteria and for antibiotic susceptibility testing

    Authors:
    Yu-Ting Kao, Tomasz S. Kaminski, Witold Postek, Jan Guzowski, Karol Makuch, Artur Ruszczak, Felix von Stetten, Roland Zengerle, Piotr Garstecki
    Academic press:
    Lab on a Chip (year: 2020, volume: 20, pages: 54-63), Publisher: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C9LC00684B – link to the publication
  18. A method for simultaneous polishing and hydrophobization of polycarbonate for microfluidic applications

    Authors:
    Dominika Ogończyk, Paweł Jankowski, Piotr Garstecki
    Academic press:
    Polymers (year: 2020, volume: 12(11), pages: 2490), Publisher: MDPI
    Status:
    Published
    DOI:
    10.3390/polym12112490 – link to the publication
  19. Split or slip - passive generation of monodisperse double emulsions with cores of varying viscosity in microfluidic tandem step emulsification system

    Authors:
    Adam S. Opalski, Karol Makuch, Ladislav Derzsi and Piotr Garstecki
    Academic press:
    RSC Advances (year: 2020, volume: 10, pages: 23058 - 23065), Publisher: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/D0RA03007D – link to the publication
  20. Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over dynamic range of 6 logs and beyond

    Authors:
    O. Scheler, N. Pacocha, P. R. Debski, A. Ruszczak, T. S. Kaminski and P. Garstecki
    Academic press:
    Lab on a Chip (year: 2017, volume: 17, pages: 1980 - 1987), Publisher: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c7lc00206h – link to the publication
  21. Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions

    Authors:
    Adam S. Opalski, Tomasz S. Kaminski, Piotr Garstecki
    Academic press:
    KONA Powder and Particle Journal (year: 2018, volume: 46098, pages: 46044), Publisher: Hosokawa Powder Technology Foundation
    Status:
    Published
    DOI:
    10.14356/kona.2019004 – link to the publication
  22. Recent developments of microfluidics as a tool for biotechnology and microbiology

    Authors:
    Ott Scheler, Witold Postek, Piotr Garstecki
    Academic press:
    Current Opinion in Biotechnology (year: 2019, volume: 55, pages: 60-67), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.copbio.2018.08.004 – link to the publication
  23. Controlled droplet microfluidic systems for multistep chemical and biological assays

    Authors:
    Tomasz S Kaminski and Piotr Garstecki
    Academic press:
    Chemical Society Reviews (year: 2017, volume: 46, pages: 6210 - 6226), Publisher: ROYALSOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C5CS00717H – link to the publication
  24. A passive microfluidic system based on step emulsification allows the generation of libraries of nanoliter-sized droplets from microliter droplets of varying and known concentrations of a sample

    Authors:
    W. Postek, T. Kamiński, P. Garstecki
    Academic press:
    Lab on a Chip (year: 2017, volume: 17, pages: 1323-1331), Publisher: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C7LC00014F – link to the publication
  25. Antimicrobial and attractant roles for chemerin in the oral cavity during inflammatory gum disease

    Authors:
    U.Godlewska, P.Brzoza, A.Sroka, P.Majewski, H.Jentsch, M.Eckert, S.Eick, J.Potempa, B.Zabel and J.Cichy
    Academic press:
    Frontiers in Immunology (year: 2017, volume: 0,578472222222222, pages: 46033), Publisher: Frontiers
    Status:
    Published
    DOI:
    10.3389/fimmu.2017.00353 – link to the publication
  26. Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette

    Authors:
    L. Derzsi, T.S. Kaminski and P. Garstecki
    Academic press:
    Lab on a Chip (year: 2016, volume: 16, pages: 893-901), Publisher: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C5LC01151E – link to the publication
  27. Microfluidic screening of antibiotic susceptibility at a single-cell level shows inoculum effect of cefotaxime in E. coli

    Authors:
    Witold Postek, Pawel Gargulinski, Ott Scheler, Tomasz S. Kaminski and Piotr Garstecki
    Academic press:
    Lab on a Chip (year: 2018, volume: 18, pages: 3668–3677), Publisher: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C8LC00916C – link to the publication
  28. Oscillating droplet trains in microfluidic networks and their suppression in blood flow

    Authors:
    Bartosz Grzybowski, Olgierd Cybulski, Piotr Garstecki
    Academic press:
    Nature Physics (year: 2019, volume: 15, pages: 706-713), Publisher: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41567-019-0486-8 – link to the publication
  29. Passive and parallel microfluidic formation of droplet interface bilayers (DIBs) for measurement of leakage of small molecules through artificial phospholipid membranes

    Authors:
    Magdalena A. Czekalska, Tomasz S. Kaminski, Karol Makuch and Piotr Garstecki
    Academic press:
    Sensors and Actuators: B Chemical (year: 2019, volume: 286, pages: 258-265), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.snb.2019.01.143 – link to the publication
  30. A precise and accurate microfluidic droplet dilutor

    Authors:
    Witold Postek, Tomasz S Kaminski and Piotr Garstecki
    Academic press:
    Analyst (year: 2017, volume: 142, pages: 2901-2911), Publisher: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C7AN00679A – link to the publication
  31. Grooved step emulsification systems optimize throughput of passive generation of monodisperse emulsions

    Authors:
    Adam S. Opalski, Karol Makuch, Yu-Kai Lai, Ladislav Derzsi and Piotr Garstecki
    Academic press:
    Lab on a Chip (year: 2019, volume: 19, pages: 1183-1192), Publisher: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C8LC01096J – link to the publication
  32. The antimicrobial activity of chemerin-derived peptide p4 requires oxidative conditions.

    Authors:
    Urszula Godlewska, Bernadetta Bilska, Aneta Zegar, Piotr Brzoza, Arkadiusz Borek, Krzysztof Murzyn, Oliwia Bochenska, Agnieszka Morytko, Patryk Kuleta, Andrzej Kozik, Elzbieta Pyza, Artur Osyczka, Brian A Zabel and Joanna Cichy
    Academic press:
    Journal of Biological Chemistry (year: 2019, volume: 294, pages: 1267-1278), Publisher: American Society for Biochemistry and Molecular Biology Privacy Policy
    Status:
    Published
    DOI:
    10.1074/jbc.RA118.005495 – link to the publication

« New search

Graphic design and implementation: Jakub Piotrowski