The role of antimicrobial protein-chemerin in skin pathophysiology.
2014/12/W/NZ6/00454
Keywords
Descriptors
- NZ6_4: Bakteriologia
- NZ4_4: Patofizjologia ogólna
Panel
NZ6 – Human and animal immunology and infection: immunity, immune disorders, immunotherapy, infectious and invasive diseases, microbiology, transplantology, allergology
Host institution
Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii
Voivodeship: małopolskie
Principal investigator (from the host institution)
Number of co-investigators in the project: 18
Call
SYMFONIA 2 – announced on 2013-12-16
- Amount awarded
- 6 589 560 PLN
- Project start date
- 2014-11-21
- Project end date
- 2021-02-20
- Project duration
- 75 months (the same as in the proposal)
- Project status
- Project settled
Equipment purchased [PL]
- Aparat do kawitacji.
- Komputer stacjonarny. Cost: 3 676 PLN
- Komputer stacjonarny.
- Mikroskop biologiczny z kamerą cyfrową. Cost: 16 728 PLN
- Platforma PCR do analiz w czasie rzeczywistym.. Cost: 180 000 PLN
- Pompa strzykawkowe i robot do nanoszenia próbek.. Cost: 60 000 PLN
- Przyrząd do mierzenia wilgotości skóry.. Cost: 75 000 PLN
- Rozbudowa stacji roboczej (hardware i software).
- Stacja robocza i podajnik płytek do cytometru przepływowego LSRII.. Cost: 140 000 PLN
- Stacja robocza i rozbudowa cytometru o nowe lasery.
- System do analizy obrazów.. Cost: 170 000 PLN
- System do detekcji produktów PCR w czasie rzeczywistym.. Cost: 120 000 PLN
- Wielodetekcyjny czytnik mikropłytkowy. Cost: 100 000 PLN
- Zamrażarka. Cost: 849 PLN
- dwustanowiskowa komora laminarna.
- komputer stacjonarny - 2 szt..
- komputer stacjonarny z monitorem LCD.
- Łaźnia wodna. Cost: 5 227 PLN
Information in the final report
Publication in academic press/journals (32)
-
Dodecylresorufin (C12R) outperforms resorufin in micro-droplet bacterial assays
- Authors:
- O. Scheler, T.S. Kaminski, A. Ruszczak and P. Garstecki
- Academic press:
- Applied Materials and Interfaces (year: 2016, volume: 8, pages: 11318-11325), Publisher: AMER CHEMICAL SOC
- Status:
- Published
- DOI:
- 10.1021/acsami.6b02360 – link to the publication
-
Droplet microfluidics for microbiology: techniques, applications and challenges
- Authors:
- T.S. Kaminski, O. Scheler and P. Garstecki
- Academic press:
- Lab on a Chip (year: 2016, volume: 16, pages: 2168-2187), Publisher: ROYAL SOC CHEMISTRY
- Status:
- Published
- DOI:
- 10.1039/C6LC00367B – link to the publication
-
The expression and regulation of chemerin in the epidermis.
- Authors:
- Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel BA, Cichy J.
- Academic press:
- PLos One (year: 2015, volume: 10(2):e011783, pages: 46041), Publisher: Plos.org
- Status:
- Published
- DOI:
- 10.1371/journal.pone.0117830 – link to the publication
-
The methylation status of the chemerin promoter region located from - 252 to + 258 bp regulates constitutive but not acute-phase cytokine-inducible chemerin expression levels
- Authors:
- Kwiecien K, Brzoza P, Bak M, Majewski P, Skulimowska I, Bednarczyk K, Cichy J, Kwitniewski M.
- Academic press:
- Scientific Reports (year: 2020, volume: 10, pages: 13702 (1-12)), Publisher: Nature
- Status:
- Published
- DOI:
- 10.1038/s41598-020-70625-7 – link to the publication
-
Bacteria Modify Their Sensitivity to Chemerin-Derived Peptides by Hindering Peptide Association With the Cell Surface and Peptide Oxidation
- Authors:
- Godlewska U, Bilska B, Majewski P, Pyza E, Zabel BA, Cichy J.
- Academic press:
- Frontiers in Microbiology (year: 2020, volume: 11, pages: 1819 (1-11)), Publisher: Frontiers
- Status:
- Published
- DOI:
- 10.3389/fmicb.2020.01819 – link to the publication
-
Expression of antimicrobial peptide genes oscillates along day/night rhythm protecting mice skin from bacteria
- Authors:
- Bilska B, Zegar A, Slominski AT, Kleszczyński K, Cichy J, Pyza E.
- Academic press:
- Experimental Dermatology (year: 2020, volume: 0, pages: 46032), Publisher: John Wiley & Sons Ltd
- Status:
- Published
- DOI:
- 10.1111/exd.14229 – link to the publication
-
Metagenomic Studies in Inflammatory Skin Diseases
- Authors:
- Godlewska U, Brzoza P, Kwiecień K, Kwitniewski M, Cichy J.
- Academic press:
- Current Microbiology (year: 2020, volume: 77, pages: 3201–3212), Publisher: Springer nature
- Status:
- Published
- DOI:
- 10.1007/s00284-020-02163-4 – link to the publication
-
Direct droplet digital PCR (dddPCR) for species specific, accurate and precise quantification of bacteria in mixed samples
- Authors:
- Natalia Pacocha, Ott Scheler, Mikołaj Marcin Nowak, Ladislav Derzsi, Joanna Cichy, Piotr Garstecki
- Academic press:
- Analytical Methods (year: 2019, volume: 11, pages: 5730-5735), Publisher: ROYAL SOC CHEMISTRY
- Status:
- Published
- DOI:
- 10.1039/C9AY01874C – link to the publication
-
Non-wetting droplets in capillaries of circular cross-section: scaling function
- Authors:
- Karol Makuch, Jean-Baptiste Gorce, and Piotr Garstecki
- Academic press:
- Physics of Fluids (year: 2019, volume: 31, pages: 43102), Publisher: AIP Publishing
- Status:
- Published
- DOI:
- 10.1063/1.5087722 – link to the publication
-
The role of chemerin and its antibacterial derivatives in maintaining epithelial barrier function
- Authors:
- Godlewska U, Zegar A, Cichy J.
- Academic press:
- Postepy Biochemii (year: 2020, volume: 66 (2), pages: 151-159), Publisher: Polskie Towarzystwo Biochemiczne
- Status:
- Published
- DOI:
- 10.18388/pb.2020_323 – link to the publication
-
Architecture of antimicrobial skin defense.
- Authors:
- Kwiecien K, Zegar A, Jung J, Brzoza P, Kwitniewski M, Godlewska U, Grygier B, Kwiecinska P, Morytko A, Cichy J.
- Academic press:
- Cytokine & Growth Factor Reviews (year: 2019, volume: 49, pages: 70-84), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.cytogfr.2019.08.001 – link to the publication
-
Redox Active Antimicrobial Peptides in Controlling Growth of Microorganisms at Body Barriers
- Authors:
- Brzoza, P.; Godlewska, U.; Borek, A.; Morytko, A.; Zegar, A.; Kwiecinska, P.; Zabel, B.A.; Osyczka, A.; Kwitniewski, M.; Cichy, J.
- Academic press:
- Antioxidants (year: 2021, volume: 10(3), pages: 446 (1-18)), Publisher: MDPI
- Status:
- Published
- DOI:
- link to the publication
-
High-Throughput Monitoring of Bacterial Cell Density in Nanoliter Droplets: Label-Free Detection of Unmodified Gram-Positive and Gram-Negative Bacteria
- Authors:
- Natalia Pacocha, Jakub Bogusławski, Michał Horka, Karol Makuch,Kamil Liżewski, Maciej Wojtkowski, Piotr Garsteck
- Academic press:
- Analytical Chemistr (year: 2021, volume: 93, 2, pages: 843–850), Publisher: American Chemical Society
- Status:
- Published
- DOI:
- 10.1021/acs.analchem.0c03408 – link to the publication
-
Antimicrobial Properties of a Peptide Derived from the Male Fertility Factor kl2 Protein of Drosophila melanogaster
- Authors:
- Bilska, B.; Godlewska, U.; Damulewicz, M.; Murzyn, K.; Kwitniewski, M.; Cichy, J.; Pyza, E.
- Academic press:
- Current Issues in Molecular Biology (year: 2022, volume: 44, pages: 1169-1181), Publisher: MDPI
- Status:
- Published
- DOI:
- 10.3390/cimb44030076 – link to the publication
-
Chemerin-derived peptide Val66-Pro85 is effective in limiting methicillin-resistant S. aureus skin infection.
- Authors:
- Aneta Zegar, Urszula Godlewska, Dorota Kozłowska-Chmielewska, Pawel Majewski, Brian A Zabel, Joanna Cichy
- Academic press:
- Frontiers in Microbiology (year: 2021, volume: 12, pages: 46030), Publisher: Frontiers Media SA
- Status:
- Published
- DOI:
- 10.3389/fmicb.2021.742610 – link to the publication
-
Droplet-based digital antibiotic susceptibility screen reveals single-cell clonal heteroresistance in an isogenic bacterial population
- Authors:
- Ott Scheler, Karol Makuch, Pawel R Debski, Michal Horka, Artur Ruszczak, Natalia Pacocha, Krzysztof Sozanski, Olli-Pekka Smolander, Witold Postek, Piotr Garstecki
- Academic press:
- Scientific Reports (year: 2020, volume: 10, pages: 3282), Publisher: Springer Nature
- Status:
- Published
- DOI:
- 10.1038/s41598-020-60381-z – link to the publication
-
Gravity-driven microfluidic assay for digital enumeration of bacteria and for antibiotic susceptibility testing
- Authors:
- Yu-Ting Kao, Tomasz S. Kaminski, Witold Postek, Jan Guzowski, Karol Makuch, Artur Ruszczak, Felix von Stetten, Roland Zengerle, Piotr Garstecki
- Academic press:
- Lab on a Chip (year: 2020, volume: 20, pages: 54-63), Publisher: The Royal Society of Chemistry
- Status:
- Published
- DOI:
- 10.1039/C9LC00684B – link to the publication
-
A method for simultaneous polishing and hydrophobization of polycarbonate for microfluidic applications
- Authors:
- Dominika Ogończyk, Paweł Jankowski, Piotr Garstecki
- Academic press:
- Polymers (year: 2020, volume: 12(11), pages: 2490), Publisher: MDPI
- Status:
- Published
- DOI:
- 10.3390/polym12112490 – link to the publication
-
Split or slip - passive generation of monodisperse double emulsions with cores of varying viscosity in microfluidic tandem step emulsification system
- Authors:
- Adam S. Opalski, Karol Makuch, Ladislav Derzsi and Piotr Garstecki
- Academic press:
- RSC Advances (year: 2020, volume: 10, pages: 23058 - 23065), Publisher: The Royal Society of Chemistry
- Status:
- Published
- DOI:
- 10.1039/D0RA03007D – link to the publication
-
Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over dynamic range of 6 logs and beyond
- Authors:
- O. Scheler, N. Pacocha, P. R. Debski, A. Ruszczak, T. S. Kaminski and P. Garstecki
- Academic press:
- Lab on a Chip (year: 2017, volume: 17, pages: 1980 - 1987), Publisher: ROYAL SOC CHEMISTRY
- Status:
- Published
- DOI:
- 10.1039/c7lc00206h – link to the publication
-
Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions
- Authors:
- Adam S. Opalski, Tomasz S. Kaminski, Piotr Garstecki
- Academic press:
- KONA Powder and Particle Journal (year: 2018, volume: 46098, pages: 46044), Publisher: Hosokawa Powder Technology Foundation
- Status:
- Published
- DOI:
- 10.14356/kona.2019004 – link to the publication
-
Recent developments of microfluidics as a tool for biotechnology and microbiology
- Authors:
- Ott Scheler, Witold Postek, Piotr Garstecki
- Academic press:
- Current Opinion in Biotechnology (year: 2019, volume: 55, pages: 60-67), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.copbio.2018.08.004 – link to the publication
-
Controlled droplet microfluidic systems for multistep chemical and biological assays
- Authors:
- Tomasz S Kaminski and Piotr Garstecki
- Academic press:
- Chemical Society Reviews (year: 2017, volume: 46, pages: 6210 - 6226), Publisher: ROYALSOC CHEMISTRY
- Status:
- Published
- DOI:
- 10.1039/C5CS00717H – link to the publication
-
A passive microfluidic system based on step emulsification allows the generation of libraries of nanoliter-sized droplets from microliter droplets of varying and known concentrations of a sample
- Authors:
- W. Postek, T. Kamiński, P. Garstecki
- Academic press:
- Lab on a Chip (year: 2017, volume: 17, pages: 1323-1331), Publisher: ROYAL SOC CHEMISTRY
- Status:
- Published
- DOI:
- 10.1039/C7LC00014F – link to the publication
-
Antimicrobial and attractant roles for chemerin in the oral cavity during inflammatory gum disease
- Authors:
- U.Godlewska, P.Brzoza, A.Sroka, P.Majewski, H.Jentsch, M.Eckert, S.Eick, J.Potempa, B.Zabel and J.Cichy
- Academic press:
- Frontiers in Immunology (year: 2017, volume: 0,578472222222222, pages: 46033), Publisher: Frontiers
- Status:
- Published
- DOI:
- 10.3389/fimmu.2017.00353 – link to the publication
-
Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette
- Authors:
- L. Derzsi, T.S. Kaminski and P. Garstecki
- Academic press:
- Lab on a Chip (year: 2016, volume: 16, pages: 893-901), Publisher: ROYAL SOC CHEMISTRY
- Status:
- Published
- DOI:
- 10.1039/C5LC01151E – link to the publication
-
Microfluidic screening of antibiotic susceptibility at a single-cell level shows inoculum effect of cefotaxime in E. coli
- Authors:
- Witold Postek, Pawel Gargulinski, Ott Scheler, Tomasz S. Kaminski and Piotr Garstecki
- Academic press:
- Lab on a Chip (year: 2018, volume: 18, pages: 3668–3677), Publisher: Royal Society of Chemistry
- Status:
- Published
- DOI:
- 10.1039/C8LC00916C – link to the publication
-
Oscillating droplet trains in microfluidic networks and their suppression in blood flow
- Authors:
- Bartosz Grzybowski, Olgierd Cybulski, Piotr Garstecki
- Academic press:
- Nature Physics (year: 2019, volume: 15, pages: 706-713), Publisher: Springer Nature
- Status:
- Published
- DOI:
- 10.1038/s41567-019-0486-8 – link to the publication
-
Passive and parallel microfluidic formation of droplet interface bilayers (DIBs) for measurement of leakage of small molecules through artificial phospholipid membranes
- Authors:
- Magdalena A. Czekalska, Tomasz S. Kaminski, Karol Makuch and Piotr Garstecki
- Academic press:
- Sensors and Actuators: B Chemical (year: 2019, volume: 286, pages: 258-265), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.snb.2019.01.143 – link to the publication
-
A precise and accurate microfluidic droplet dilutor
- Authors:
- Witold Postek, Tomasz S Kaminski and Piotr Garstecki
- Academic press:
- Analyst (year: 2017, volume: 142, pages: 2901-2911), Publisher: ROYAL SOC CHEMISTRY
- Status:
- Published
- DOI:
- 10.1039/C7AN00679A – link to the publication
-
Grooved step emulsification systems optimize throughput of passive generation of monodisperse emulsions
- Authors:
- Adam S. Opalski, Karol Makuch, Yu-Kai Lai, Ladislav Derzsi and Piotr Garstecki
- Academic press:
- Lab on a Chip (year: 2019, volume: 19, pages: 1183-1192), Publisher: Royal Society of Chemistry
- Status:
- Published
- DOI:
- 10.1039/C8LC01096J – link to the publication
-
The antimicrobial activity of chemerin-derived peptide p4 requires oxidative conditions.
- Authors:
- Urszula Godlewska, Bernadetta Bilska, Aneta Zegar, Piotr Brzoza, Arkadiusz Borek, Krzysztof Murzyn, Oliwia Bochenska, Agnieszka Morytko, Patryk Kuleta, Andrzej Kozik, Elzbieta Pyza, Artur Osyczka, Brian A Zabel and Joanna Cichy
- Academic press:
- Journal of Biological Chemistry (year: 2019, volume: 294, pages: 1267-1278), Publisher: American Society for Biochemistry and Molecular Biology Privacy Policy
- Status:
- Published
- DOI:
- 10.1074/jbc.RA118.005495 – link to the publication