Projects funded by the NCN
Molecular determinants of interactions between GW182 and CNOT1, proteins involved in miRNA-mediated silencing - identification of key protein fragments and their conformational changes determining molecular recognition.
2013/11/N/NZ1/02387
Keywords:
miRNA regulation of gene expression GW182 TNRC6C silencing domain CNOT1 protein-protein interactions mass spectrometry natively unstructured proteins hydrogen-deuterium exchange conformational changes
Descriptors:
- NZ1_1: Molecular biology
- NZ1_3: Biophysics
- NZ1_2: Biochemistry
Panel:
NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology
Host institution :
Uniwersytet Warszawski, Wydział Fizyki
woj. mazowieckie
Principal investigator (from the host institution):
Number of co-investigators in the project: 3
Call: PRELUDIUM 6 - announced on 2013-09-16
Amount awarded: 99 970 PLN
Project start date (Y-m-d): 2014-09-10
Project end date (Y-m-d): 2017-09-09
Project duration:: 36 months (the same as in the proposal)
Project status: Project settled
Equipment purchased [PL]
- oprogramowanie GraphPad Prism 6.
Information in the final report
- Publication in academic press/journals (1)
- Structural dynamics of the GW182 silencing domain including its RNA recognition motif (RRM) revealed by hydrogen-deuterium exchange mass spectrometryAuthors:Maja K. Cieplak-Rotowska, Krzysztof Tarnowski, Marcin Rubin, Marc R. Fabian, Nahum Sonenberg, Michal Dadlez, Anna NiedzwieckaAcademic press:Journal of The American Society for Mass Spectrometry (rok: 2018, tom: 29, strony: 158-173), Wydawca: SpringerStatus:PublishedDOI:10.1007/s13361-017-1830-9 - link to the publication