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Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Mechanism and consequences of protein N-homocysteinylation in humans

2013/11/B/NZ1/00091

Keywords:

homocysteine S-nitrosohomocysteine protein biosynthesis post translational modification stroke

Descriptors:

  • NZ5_3: Pathogenesis of human diseases
  • NZ5_1: Etiology of human diseases
  • NZ1_2: Biochemistry

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Przyrodniczy w Poznaniu, Wydział Rolnictwa i Bioinżynierii

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Hieronim Jakubowski 

Number of co-investigators in the project: 7

Call: OPUS 6 - announced on 2013-09-16

Amount awarded: 1 150 060 PLN

Project start date (Y-m-d): 2014-07-28

Project end date (Y-m-d): 2019-04-27

Project duration:: 57 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Jednokanalowy spektrofotometr plytkowy - zakres 200-999 nm, funkcja inkubacji i wytrzasania, notebook do kontroli (69 000 PLN)
  2. Komputery (2 szt.) (10 000 PLN)
  3. System dokumentacji chemiluminescencji.
  4. Spektrofotometr do bezposredniej detekcji (75 000 PLN)
  5. Aspirator próżniowy.
  6. Inkubator CO2.

Information in the final report

  • Publication in academic press/journals (21)
  • Articles in post-conference publications (20)
  1. Dysregulation of Epigenetic Mechanisms of Gene Expression in the Pathologies of Hyperhomocysteinemia
    Authors:
    Perła-Kaján J, Jakubowski H.
    Academic press:
    Int J Mol Sci (rok: 2019, tom: 20(13), strony: 3140), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms20133140. - link to the publication
  2. Homocysteine modification in protein structure/function and human disease.
    Authors:
    Jakubowski H.
    Academic press:
    Physiological Reviews (rok: 2019, tom: 99(1), strony: 555-604), Wydawca: American Physiological Society
    Status:
    Published
    DOI:
    10.006 - link to the publication
  3. l-Arginine and vitamin C attenuate pro-atherogenic effects of high-fat diet on biomarkers of endothelial dysfunction in rats
    Authors:
    Bogdański P, Suliburska J, Szulińska M, Sikora M, Walkowiak J, Jakubowski H.
    Academic press:
    Biomedicine and Pharmacotherapy (rok: 2015, tom: 76, strony: 100-106), Wydawca: Elsevier Science
    Status:
    Published
    DOI:
    10.001 - link to the publication
  4. Mutations in Homocysteine Metabolism Genes Increase N-Homocysteinylation and Keratin Damage in Mice
    Authors:
    Borowczyk K, Wróblewski J, Suliburska JM, Akahoshi N, Ishii I, Jakubowski H.
    Academic press:
    International Journal of Genomics (rok: 2018, tom: Article 7570850, strony: 7 pages), Wydawca: Hindawi
    Status:
    Published
    DOI:
    10.1155/2018/7570850 - link to the publication
  5. Serum Proteome Alterations in Human Cystathionine β-Synthase Deficiency and Ischemic Stroke Subtypes
    Authors:
    Sikora M, Lewandowska I, Kupc M, Kubalska J, Graban A, Marczak Ł, Kaźmierski R, Jakubowski H.
    Academic press:
    Int J Mol Sci (rok: 2019, tom: 20(12), strony: E3096), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms20123096 - link to the publication
  6. Homocysteine Editing, Thioester Chemistry, Coenzyme A, and the Origin of Coded Peptide Synthesis
    Authors:
    Jakubowski Hieronim
    Academic press:
    Life (rok: 2017, tom: 7, strony: 45316), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/life7010006 - link to the publication
  7. The amino acid metabolite homocysteine activates mTORC1 to inhibit autophagy and form abnormal proteins in human neurons and mice
    Authors:
    Khoosheh Khayati, Henri Antikainen, Edward M. Bonder, Gregory F. Weber, Warren D. Kruger, Hieronim Jakubowski, Radek Dobrowolski,
    Academic press:
    FASEB Journal (rok: 2017, tom: 31, strony: 598-609), Wydawca: FASEB
    Status:
    Published
    DOI:
    10.1096/fj.201600915R - link to the publication
  8. Aminoacyl-tRNA synthetases and the evolution of coded protein synthesis: the Thioester World.
    Authors:
    Jakubowski H.
    Academic press:
    FEBS Lett. (rok: 2016, tom: 590, strony: 469-81), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/1873-3468.12085 - link to the publication
  9. Demethylation of methionine and keratin damage in human hair
    Authors:
    Borowczyk K, Suliburska J, Jakubowski H.
    Academic press:
    Amino Acids (rok: 2018, tom: 50, strony: 537-546), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00726-018-2545-3 - link to the publication
  10. Effects of endurance and endurance and endurance-strenght exercise on biochemical parameters of liver function in women with abdominal obesity
    Authors:
    Skrypnik D., Ratajczak. M., Karolkiewicz I., Madry E., Pupek-Musialik D., Hansdorfer-Korzon R., Walkowiak. I. Jakubowski H., Bogdanski P.
    Academic press:
    Biomedicine and Pharmacotherapy (rok: 2016, tom: 80, strony: 45298), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.biopha.2016.02.017 - link to the publication
  11. Garlic extract favorably modifies markers of endothelial function in obese patients – randomized double blind placebo-controlled nutritional intervention.
    Authors:
    Szulinska M, Kręgielska-NarożnaM, ŚwiątekJ, Styś P, Kuźniar-Kamińska B, Jakubowski H*, Walkowiak J, Bogdański P.
    Academic press:
    Biomedicine and Pharmacotherapy (rok: 2018, tom: 102, strony: 792-797), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.biopha.2018.03.131 - link to the publication
  12. Quantification of homocysteine and cysteine by derivatization with pyridoxal 5'-phosphate and hydrophilic interaction liquid chromatography.
    Authors:
    Glowacki R, Stachniuk J, Borowczyk K, Jakubowski H.
    Academic press:
    Analytical Bioanalytical Chemistry (rok: 2016, tom: 408, strony: 1935-41), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-016-9308-3 - link to the publication
  13. Quantification of urinary S- and N- homocysteinylated protein and homocysteine-thiolactone in mice
    Authors:
    Jakubowski H
    Academic press:
    Analytical Biochemistry (rok: 2016, tom: 508, strony: 118-123), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ab.2016.06.002 - link to the publication
  14. Sex affects N-homocysteinylation at lysine residue 212 of albumin in mice.
    Authors:
    Sikora M, Marczak Ł, Perła-Kajan J, Jakubowski H.
    Academic press:
    Scientific Reports (rok: 2019, tom: 9(1), strony: 2669), Wydawca: Nature Springer
    Status:
    Published
    DOI:
    10.1038/s41598-019-38784-4 - link to the publication
  15. Urinary Excretion of Homocysteine Thiolactone and the Risk of Acute Myocardial Infarction in Coronary Artery Disease Patients; the WENBIT trial.
    Authors:
    Borowczyk K, Piechocka J, Głowacki R, Dahr I, Midttun Ø, Tell G, Ueland PM, Nygård O, Jakubowski H.
    Academic press:
    Journal of Internal Medicine (rok: 2019, tom: 285, strony: 232-244), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1111/joim.12834 - link to the publication
  16. Filaggrin expression and processing deficiencies impair corneocyte surface texture and stiffness in mice
    Authors:
    Thyssen JP, Jakasa I, Riethmüller C, Schön MP, Braun A, Haftek M, Fallon PG, Wróblewski J, Jakubowski H, Eckhart L, Declercq W, Koppes S, Engebretsen KA, Bonefeld C, Irvine AD, Keita-Alassane S, Simon M, Kawasaki H, Kubo A, Amagai M, Matsui T, Kezic S.
    Academic press:
    J Invest Dermatol (rok: 2020, tom: 140(3), strony: 615-623), Wydawca: ELSEVIER
    Status:
    Published
    DOI:
    10.1016/j.jid.2019.07.716. - link to the publication
  17. Homocysteine-Thiolactone and N-Homocysteinylated Protein Induce Pro-atherogenic Changes in Gene Expression in Human Vascular Endothelial Cells
    Authors:
    Dorota Gurda, Luisa Handschuh, Weronika Kotkowiak, Hieronim Jakubowski
    Academic press:
    Amino Acids (rok: 2015, tom: 47 (7), strony: 1319-39), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00726-015-1956-7 - link to the publication
  18. N-Homocysteinylation impairs collagen cross-linking in cystathionine β-synthase-deficient mice: a novel mechanism of connective tissue abnormalities
    Authors:
    Perła-Kajan J., Utyro O.,Rusek M., Malinowska A. , Sitkiewicz E. , Jakubowski H.
    Academic press:
    FASEB Journal (rok: 2016, tom: 30, strony: 3810-3821), Wydawca: FASEB
    Status:
    Published
    DOI:
    10.1096/fj.201600539 - link to the publication
  19. Paraoxonase 1 Q192R genotype and activity affect homocysteine thiolactone levels in humans.
    Authors:
    Perła-Kaján J, Borowczyk K, Głowacki R, Nygård O, Jakubowski H.
    Academic press:
    FASEB Journal (rok: 2018, tom: 32, strony: 6019-6024), Wydawca: Federation of American Societies for Experimental Biology
    Status:
    Published
    DOI:
    10.1096/fj.201800346R - link to the publication
  20. Protein N-Homocysteinylation and Colorectal Cancer.
    Authors:
    Jakubowski H.
    Academic press:
    Trends in Cancer (rok: 2019, tom: 5(1), strony: 45483), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.trecan.2018.10 - link to the publication
  21. Simultaneous Determination of Methionine and Homocysteine by on-column derivatization with o-phtaldialdehyde
    Authors:
    Borowczyk K, Chwatko G, Kubalczyk P, Jakubowski H, Kubalska J, Głowacki R
    Academic press:
    Talanta (rok: 2016, tom: 161, strony: 917-924), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.talanta.2016.09.039 - link to the publication
  1. Correlations between sulfur-containing amino acids and albumin in human plasma
    Authors:
    Borowczyk K, Jakubowski H, Głowacki R.
    Conference:
    10th Intl Conference on One Carbon Metabolism, Vitamins B and Homocysteine, Nancy, France (rok: 2015, ), Wydawca: Medical School, University of Lorraine
    Data:
    konferencja 7-11.07
    Status:
    Published
  2. Identification of 2-pyridoxyl-1,3-thiazolidine-4-carboxylic acid, novel metabolite of vitamin B6 and cysteine in human plasma
    Authors:
    Rafał Głowacki, Monika Wyszczelska-Rokiel, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochimica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  3. N-homocysteinylation as a mechanism of connective tissue impairment in hyperhomocysteinemia due to cystathionine β-synthase deficiency
    Authors:
    Joanna Perła-Kajan, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  4. N-homocysteinylation impairs collagen cross-linking in cystathionine β-synthasedeficient mice: a novel mechanism of connective tissue abnormalities
    Authors:
    Perła-Kajan J., Rusek M., Malinowska A. , Sitkiewicz E., Utyro O., Jakubowski H.
    Conference:
    2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics BIO2016 Wrocław 2016 (rok: 2016, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 13-16.09.
    Status:
    Published
  5. Paraoxonase 1 (Pon1) regulates water homeostasis by controlling the expression of Fxr and Aqp2 proteins in mice.
    Authors:
    Wieloch M, Jakubowski H.
    Conference:
    29th Annual Symposium of the Protein-Society, Barcelona (rok: 2015, ), Wydawca: Protein Science
    Data:
    konferencja 22-25.07
    Status:
    Published
  6. Paraoxonase 1 protects against homocysteine-thiolactone accumulation in humans
    Authors:
    Perła-Kajan J, Borowczyk K, Głowacki R, Nygård O, Ueland PM, Jakubowski H.
    Conference:
    11th International Conference on Homocysteine and One-Carbon Metabolism, Aarhus, Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  7. Cystathionine-β synthase deficiency changes expression of major urinary proteins in mice
    Authors:
    Jacek Wróblewski, Joanna Perła-Kaján, Monika Wyszczelska-Rokiel, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  8. Dietary hyperhomocysteinemia upregulates mTOR signaling and downregulates autophagy in the mouse brain
    Authors:
    Suszyńska-Zajczyk J. Jakubowski H.
    Conference:
    2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics BIO2016 Wrocław 2016 (rok: 2016, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 13-16.09.
    Status:
    Published
  9. Identification of N-homocysteinylation sites in mouse plasma albumin and hemoglobin from cystathionine β-synthase deficient mice.
    Authors:
    Sikora M, Marczak L, Jakubowski H.
    Conference:
    10th Intl Conference on One Carbon Metabolism, Vitamins B and Homocysteine", Nancy, France (rok: 2015, ), Wydawca: Medical School, University of Lorraine
    Data:
    konferencja 7-11.07
    Status:
    Published
  10. N - homocysteinylation impairs collagen cross - linking in cystathionine β -synthase -deficient mice: a novel mechanism of connective tissue abnormalities
    Authors:
    Joanna Perła - Kajan, Marta Rusek, Agata Malinowska, Ewa Sitkiewicz, Olga Utyro , and Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One-Carbon Metabolism, Aarhus, Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  11. Does hyperhomocysteinemia affect collagen and elastin cross-links in a mouse model of cystathionine β-synthase deficiency
    Authors:
    Perła-Kajan J, Jakubowski H.
    Conference:
    10th Intl Conference on One Carbon Metabolism, Vitamins B and Homocysteine", Nancy, France (rok: 2015, ), Wydawca: Medical School, University of Lorraine
    Data:
    konferencja 7-11.07
    Status:
    Published
  12. Label-free proteomic analysis reveals changes in red-ox and lipid homeostasis in the liver of cystathionine β-synthase deficient mice
    Authors:
    Bielińska I., Marczak Ł., Jakubowski H.
    Conference:
    2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics BIO2016 Wrocław 2016 (rok: 2016, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 13-16.09.
    Status:
    Published
  13. Methionine demethylation and protein damage: time - dependent accumulation of homocysteine in human hair keratin
    Authors:
    Kamila Borowczyk, Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One-Carbon Metabolism, Aarhus, Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  14. Cystathionine ß-synthase deficiency induces changes in the mouse brain proteome associated with neurodegenerative disease
    Authors:
    Izabela Bielińska, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One Carbon Metabolism, Aarhus , Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18. 05
    Status:
    Published
    DOI:
    link to the publication
  15. Cystathionine β-synthase deficiency elevates N-homocysteinlation at specyfic lysine residues in fibrinogen and serum albumin in mice
    Authors:
    Sikora M., Marczak Ł., Jakubowski H.
    Conference:
    2nd Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics BIO2016 Wrocław 2016 (rok: 2016, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 13-16.09.
    Status:
    Published
  16. Histones are targeted for N-homocysteinylation in human endothelial cells
    Authors:
    Dorota Gurda, Łukasz Marczak, Hieronim Jakubowski
    Conference:
    1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics "BIO 2014" Warsaw 2014 (rok: 2014, ), Wydawca: Acta Biochmica Polonica
    Data:
    konferencja 9-12.09
    Status:
    Published
    DOI:
    link to the publication
  17. Homocysteine-thiolactone and cardiovascular disease in B-vitamin trials
    Authors:
    Borowczyk K, Głowacki R, Nygård O, Jakubowski H.
    Conference:
    10th Intl Conference on One Carbon Metabolism, Vitamins B and Homocysteine", Nancy, France (rok: 2015, ), Wydawca: Medical School, University of Lorraine
    Data:
    konferencja 7-11.07
    Status:
    Published
  18. Label-free proteomic analysis of plasma of cystathionine β-synthase deficient mice and humans reveals changes in blood coagulation, hemostasis and antioxidant activity
    Authors:
    Marta Sikora,Izabela Bielińska ,Łukasz Marczak, Hieronim Jakubowski
    Conference:
    11th International Conference on Homocysteine and One Carbon Metabolism, Aarhus , Dania (rok: 2017, ), Wydawca: Aarhus University
    Data:
    konferencja 14-18.05
    Status:
    Published
    DOI:
    link to the publication
  19. Tissue-specific effect of hyperhomocysteinemia on collagen and elastin cross-links in a cystathionine β-synthase-deficient mouse.
    Authors:
    Perła-Kajan J, Ogrodna K, Jakubowski H.
    Conference:
    14th Intl Congress on Amino Acids, Peptides and Proteins. Vienna, Austria (rok: 2015, ), Wydawca: Amino Acids 2016;48: 635-635.
    Data:
    konferencja 3-7.08
    Status:
    Published
  20. Analysis of liver proteome in cystathionine beta-synthase deficient mice using 2D IEF/SDS-PAGE gel electrophoresis, MALDI-TOF mass spectrometry, and label-free based relative quantitative proteomics.
    Authors:
    Bielinska I, Marczak L, Jakubowski H.
    Conference:
    29th Annual Symposium of the Protein-Society, Barcelona (rok: 2015, ), Wydawca: Protein Science
    Data:
    konferencja 22-25.07
    Status:
    Published

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