Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The reorganization of the spinal neuronal network caused by electrical stimulation of the peripheral nerve in spinal rats

2013/09/B/NZ4/03306

Keywords:

spinal cord injury extensor and flexor motoneurons prioprioception neurotrophins

Descriptors:

  • NZ4_7: Neurophysiology
  • NZ4_9: Systems neurobiology
  • NZ4_10: Neuroimaging and computational neuroscience

Panel:

NZ4 - Biology of tissues, organs and organisms: morphology and functions of animal's and human's systems, organs and organisms, experimental medicine, basics of neurology

Host institution :

Instytut Biologii Doświadczalnej im. M. Nenckiego PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Małgorzata Skup 

Number of co-investigators in the project: 6

Call: OPUS 5 - announced on 2013-03-15

Amount awarded: 985 500 PLN

Project start date (Y-m-d): 2014-03-12

Project end date (Y-m-d): 2018-03-11

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Aparat do ilościowego pomiaru ekspresji mRNA w czasie rzeczywistym Real Time PCR System - ekonomiczna wersja aparatu Roche LightCycler® 480, z komputerem i systemem operacyjnym (120 000 PLN)
  2. Spektrofotometr BioPhotometer plus firmy Eppendorf (20 000 PLN)
  3. komputer do pracy z oprogramowaniem do obróbki i analizy obrazu.
  4. Mikrowirówka z chłodzeniem Laboratory Eppendorf 5430R i rotorami wymiennymi na probówki o obj. 1, 5, 15 i 50 ml (30 000 PLN)
  5. Wzmacniacz różnicowy Differential AC Amplifier, 220 V, 50 Hz, Model 1700 (8 400 PLN)
  6. oprogramowanie do analizy obróbki i analizy obrazu i analizy danych elektrofizjologicznych.
  7. Stymulator Model 2100 Isolated Pulse Stimulator o parametrach: 220V, 50Hz A-M System (9 600 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  • Articles in post-conference publications (15)
  1. Early pre- and postsynaptic decrease in glutamatergic and cholinergic signaling after spinalization is not modified when stimulating proprioceptive input to the ankle extensor alpha-motoneurons: anatomical and neurochemical study
    Authors:
    Kamil Grycz, Anna Glowacka, Benjun Ji, Julita Czarkowska-Bauch, Olga Gajewska-Wozniak, Malgorzata Skup
    Academic press:
    PLOS ONE (rok: 2019, tom: 14(9), strony: 45312), Wydawca: San Francisco, California and Cambridge, UnitedKingdom.
    Status:
    Published
    DOI:
    10.1371/journal.pone.0222849 - link to the publication
  2. Electrical stimulation of low-threshold proprioceptive fibers in the adult rat increases density of glutamatergic and cholinergic boutons on the ankle extensor α-motoneurons
    Authors:
    Olga Gajewska-Woźniak, Kamil Grycz, Julita Czarkowska-Bauch and Małgorzata Skup
    Academic press:
    PLOS ONE (rok: 2016, tom: 11, strony: 45312), Wydawca: San Francisco, California, and Cambridge, United Kingdom.
    Status:
    Published
    DOI:
    10.1371/journal.pone.0161614 - link to the publication
  3. The impact of training and neurotrophins on functional recovery after complete spinal cord transection: cellular and molecular mechanisms contributing to motor improvement.
    Authors:
    Małgorzata Skup, Ewelina Ziemlińska, Olga Gajewska-Woźniak, Rafał Płatek, Anna Maciejewska, and Julita Czarkowska-Bauch
    Academic press:
    Acta Neurobiologiae Experimentalis (rok: 2014, tom: 74, strony: 121-141), Wydawca: Nencki Institute of Experimental Biology, Polish Neuroscience Society
    Status:
    Published
    DOI:
    brak - link to the publication
  4. Spinalization and locomotor training differentially affect muscarinic acetylcholine receptor type 2 abutting on α-motoneurons innervating the ankle extensor and flexor muscles
    Authors:
    Więckowska Angelika, Gajewska-Woźniak Olga, Głowacka Anna, Ji Benjun, Grycz Kamil, Czarkowska-Bauch Julita and Skup Malgorzata
    Academic press:
    Journal of Neurochemistry (rok: 2018, tom: 147, strony: 361-379), Wydawca: WILEY-BLACKWELL
    Status:
    Published
    DOI:
    10.1111/jnc.14567 - link to the publication
  1. Early changes in gene expression of spinal perineuronal nets components in rats after complete spinal cord transection
    Authors:
    Kamil Grycz, Olga Gajewska‑Woźniak, Benjun Ji, Julita Czarkowska‑Bauch, Małgorzata Skup
    Conference:
    13 International Congress of the Polish Neuroscience Society (rok: 2017, ), Wydawca: Acta Neurobiologiae Experimentalis Vol. 77 No. S1
    Data:
    konferencja 28-31 August
    Status:
    Published
    DOI:
    link to the publication
  2. Low-threshold stimulation of proprioceptive fibers in the adult rat is a feasible tool to increase density of glutamatergic and cholinergic boutons on the ankle extensor motoneurons.
    Authors:
    Małgorzata Skup, Olga Gajewska-Woźniak., Kamil Grycz, Julita Czarkowska-Bauch
    Conference:
    FENS FORUM (rok: 2016, ), Wydawca: Federation of European Neuroscience Societies
    Data:
    konferencja 2-6 lipca
    Status:
    Published
  3. Low-treshold stimulation of the tibial nerve enriches cholinergic innervation of the ankle extensors motoneurons
    Authors:
    Olga Gajewska-Woźniak, Julita Czarkowska-Bauch, Małgorzata Skup
    Conference:
    9th FENS Forum of Neuroscience (rok: 2014, ), Wydawca: FENS
    Data:
    konferencja July 5th - 9th
    Status:
    Published
    DOI:
    link to the publication
  4. Spinal BDNF overexpression leads to opposite effects on glutamatergic AMPAR and NMDAR than on muscarinic acetylcholine receptor M2 transcript level in rats with complete spinal cord transection
    Authors:
    Benjun Ji, Anna Głowacka, Olga Gajewska‑Woźniak, Julita Czarkowska‑Bauch, Małgorzata Skup
    Conference:
    13 International Congress of the Polish Neuroscience Society (rok: 2017, ), Wydawca: Acta Neurobiologiae Experimentalis Vol. 77 No. S1
    Data:
    konferencja 28-31 August
    Status:
    Published
    DOI:
    link to the publication
  5. Spinal cord transection and stimulation of low-threshold proprioceptive fibers in the tibial nerve change expression of AMPA, NMDA and M2 receptors in GL and TA motoneurons
    Authors:
    Benjun Ji, Kamil Grycz, Anna Głowacka, Angelika Więckowska, Olga Gajewska-Woźniak, Julita Czarkowska-Bauch, Małgorzata Skup
    Conference:
    FENS FORUM (rok: 2018, ), Wydawca: Federation of European Neuroscience Societies
    Data:
    konferencja 7-11 lipca
    Status:
    Published
  6. Differential changes of expression level of Crtl1/Hapln1 link protein in ankle extensor and flexor α-motoneurons in rats after complete spinal cord transection
    Authors:
    Grycz K., Gajewska-Woźniak O., Ji B., Czarkowska-Bauch J., Skup M.
    Conference:
    FENS Forum of Neuroscience (rok: 2018, ), Wydawca: Federation of European Neuroscience Societies
    Data:
    konferencja 7-11 lipca
    Status:
    Published
    DOI:
    link to the publication
  7. Enrichment in glutamatergic and cholinergic boutons of ankle extensor α-motoneurons after low-threshold stimulation of proprioceptive fibers in the adult rat
    Authors:
    Olga Gajewska-Woźniak, Małgorzata Skup, Julita Czarkowska-Bauch
    Conference:
    12 International Congress of the Polish Neuroscience Society (rok: 2015, ), Wydawca: Acta Neurobiologiae Experimentalis 75 (3)
    Data:
    konferencja 6-8 September
    Status:
    Published
  8. Reduction in cholinergic and glutamatergic innervation of ankle extensor butnotflexormotoneuronsafterspinalizationcallsforselectivetherapies
    Authors:
    OlgaGajewska-Woźniak and Julita Czarkowska-Bauch
    Conference:
    13 International Congress of the Polish Neuroscience Society (rok: 2017, ), Wydawca: Acta Neurobiologiae Experimentalis Vol. 77 No. S1
    Data:
    konferencja 28-31 August
    Status:
    Published
    DOI:
    link to the publication
  9. The effect of low-threshold stimulation of proprioceptive fibers on glutamatergic and cholinergic innervation of the ankle extensor motoneurons after complete transection of the spinal cord.
    Authors:
    Kamil Grycz ,Anna Głowacka A., Olga Gajewska-Woźniak, Benjun Ji, Julita Czarkowka-Bauch, Małgorzata Skup.
    Conference:
    FENS FORUM (rok: 2016, ), Wydawca: Federation of European Neuroscience Societies
    Data:
    konferencja 2-6 lipca
    Status:
    Published
  10. The effect of one week of low-treshold stimulation of prioprioceptive fibers on glutamatergic and cholinergic innervation of the ankle extensor a-motoneurons on early phase after complete transection of the spinal cord
    Authors:
    Kamil Grycz, Anna Głowacka, Olga Gajewska-Woźniak, Julita Czarkowska-Bauch, Małgorzata Skup.
    Conference:
    XIII Międzynarodowe Sympozjum Neurochemiczne" Molecular basis of pathology and therapy in neurological disorders. (rok: 2016, ), Wydawca: Instytut im. M. Mossakowskiego PAN Warszawa; https://www.termedia.pl/Journal/Folia_Neuropathologica-20/Numer-3-2016
    Data:
    konferencja 17-18 listopada
    Status:
    Published
  11. Electrical stimulation can increase the density of glutamatergic and cholinergic terminals on motoneurons
    Authors:
    M.SKUP, O.GAJEWSKA-WOŻNIAK, K.GRYCZ, A.GŁOWACKA, B.JI, A.WIĘCKOWSKA, J.CZARKOWSKA-BAUCH
    Conference:
    International Motoneuron Society Conference (rok: 2018, ), Wydawca: International Motoneuron Society
    Data:
    konferencja 11-14 czerwca
    Status:
    Published
  12. Locomotor training of the spinal rats decreases abundance of GlyR anchoring gephyrin in the ankle extensor and flexor motoneurons mildly reducing perineuronal nets encapsulating them
    Authors:
    Małgorzata Skup, Anna Głowacka, Olga Gajewska-Woźniak, Julita Czarkowska-Bauch
    Conference:
    12 International Congress of the Polish Neuroscience Society (rok: 2015, ), Wydawca: Acta Neurobiologiae Experimentalis 75 (3)
    Data:
    konferencja 6-8 September
    Status:
    Published
  13. Spinal BDNF overexpression partially protects synaptic machinery of neuromuscular junction from disintegration after complete spinal cord transection in adult rats: neurochemical and morphological changes evaluated by histochemical techniques
    Authors:
    Głowacka A, Gajewska-Woźniak O, Bernadzki KM, Czarkowska-Bauch J, Skup M
    Conference:
    13 International Congress of the Polish Neuroscience Society (rok: 2017, ), Wydawca: Acta Neurobiologiae Experimentalis Vol. 77 No. S1
    Data:
    konferencja 28-31 August
    Status:
    Published
    DOI:
    link to the publication
  14. Spinalization and locomotor training affect muscarininc acetylcholine receptor type 2 differently in motoneurons innervating antagonistically acting Soleus and Tibialis ant. muscles.
    Authors:
    Angelika Więckowska, Olga Gajewska-Woźniak, Julita Czarkowska-Bauch, Małgorzata Skup
    Conference:
    FENS FORUM (rok: 2016, ), Wydawca: Federation of European Neuroscience Societies
    Data:
    konferencja 2-6 lipca
    Status:
    Published
    DOI:
    link to the publication
  15. Stimulation of low-threshold proprioceptive fibers increases density of glutamatergic and cholinergic boutons on the ankle extensor alpha-motoneurons in the adult rat.
    Authors:
    Olga Gajewska-Woźniak, Julita Czarkowska-Bauch, Kamil Grycz, Małgorzata Skup
    Conference:
    Annual Meeting of the Society for Neuroscience (rok: 2016, ), Wydawca: American Society for Neuroscience (SfN)
    Data:
    konferencja 12-16 listopada
    Status:
    Published

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