Application of hyphenated separation techniques for fractionation of nanoparticles and metabolites of antibiotic nature produced by actinomycetes of extreme environments

2013/08/W/NZ8/00701

Keywords

Descriptors

  • NZ8_10: Taksonomia i filogenetyka
  • NZ8_4: Różnorodność biologiczna
  • NZ8_13: Inne zagadnienia pokrewne

Panel

NZ8 – Evolutionary and environmental biology: evolution, ecology, population biology, biodiversity, biogeography

Host institution

Uniwersytet Mikołaja Kopernika, Wydział Chemii

Voivodeship: kujawsko-pomorskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Bogusław Andrzej Buszewski

Number of co-investigators in the project: 12

Call

SYMFONIA 1 – announced on 2012-12-15

Amount awarded
2 945 136 PLN
Project start date
2013-12-09
Project end date
2016-12-08
Project duration
36 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Automatyczny wielokrotny inokulator wraz z głowicą i igłami inokulacyjnymi, Jencons PLS. Cost: 60 000 PLN
  2. Homogenizator mechaniczny - FastPrep -24 instrument, Thermoscientific. Cost: 50 000 PLN
  3. Klimatyzator. Cost: 6 765 PLN
  4. Laboratoryjna myjka ultradźwiękowa. Cost: 6 500 PLN
  5. Niskotemperaturowa zamrażarka Forma 88000 Series, thermo Scientific. Cost: 50 000 PLN
  6. Wytrząsarka z inkubatorem wraz z platformami i uchwytami do mocowania kolb, MaxQ5000, Thermo Scientific (2 pcs). Cost: 100 000 PLN
  7. agregat chłodzący do wyparki próżniowej. Cost: 30 000 PLN
  8. chromatograf cieczowy z detektorem uniwersalnym UV-Vis oraz detektorem fluorymetrycznym. Cost: 250 000 PLN
  9. koncentrator blokowy, do odparowania próbek. Cost: 25 000 PLN
  10. liofilizator (2 pcs). Cost: 170 000 PLN
  11. lodówka. Cost: 2 000 PLN
  12. mieszadło obrotowe, plus uchwyty, Noria RD NR100DE. Cost: 8 000 PLN
  13. pompa laboratoryjna próżniowa. Cost: 3 500 PLN
  14. termostat do automatycznego podajnika. Cost: 30 000 PLN
  15. urządzenie do termostatowania, przechowywania i transportowania próbek biologicznych. Cost: 6 500 PLN
  16. waga analityczna. Cost: 20 000 PLN

Information in the final report

Publication in academic press/journals (30)

  1. Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles

    Authors:
    M. Składanowski, P. Golińska, K. Rudnicka, H. Dahm, M. Rai
    Academic press:
    Medical Microbiology and Immunology (year: 2016, volume: 205, pages: 603-613), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s00430-016-0477-7 – link to the publication
  2. Antimicrobial effectiveness of synthesized bioactive silver nanoparticles by the Actinomycetes HGG16n strain

    Authors:
    B. Buszewski, V. Railean-Plugaru, P. Pomastowski, K. Rafińska, M. Szultka-Mlynska, T. Kowalkowski
    Academic press:
    Current Pharmaceutical Biotechnology (year: 2017, volume: 18, pages: 168-176), Publisher: Bentham Science Publishers
    Status:
    Published
    DOI:
    10.2174/13892010186661701041124 – link to the publication
  3. Mycoendophytes as efficient synthesizers of bionanoparticles: nanoantimicrobials, mechanism, and cytotoxicity

    Authors:
    P. Golińska, D. Rathod, M. Wypij, I. Gupta, M. Składanowski, P. Paralikar, H. Dahm, M. Rai
    Academic press:
    Critical Review in Biotechnology (year: 2017, volume: 37, pages: 765-778), Publisher: Taylor&Francis
    Status:
    Published
    DOI:
    10.1080/07388551.2016.1235011 – link to the publication
  4. Electrochemistry-mass spectrometry for in-vitro determination of selected chemotherapeutics and their electrochemical products in comparison to in-vivo approach

    Authors:
    M. Szultka-Młyńska, B. Buszewski
    Academic press:
    Talanta (year: 2016, volume: 160, pages: 694-703), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.talanta.2016.07.054 – link to the publication
  5. Actinospica durhamensis sp. nov., isolated from a spruce forest soil

    Authors:
    P. Golińska, T.D. Zucchi, L. Silva, H. Dahm, M. Goodfellow
    Academic press:
    Antonie van Leeuwenhoek (year: 2015, volume: 108, pages: 435-442), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s10482-015-0496-1 – link to the publication
  6. Streptacidiphilus toruniensis sp. nov., isolated from a pine forest soil

    Authors:
    P. Golińska, H. Dahm, M. Goodfellow
    Academic press:
    Antonie van Leeuwenhoek (year: 2016, volume: 109, pages: 1583-1591), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s10482-016-0759-5 – link to the publication
  7. Actinobacterial-mediated synthesis of silver nanoparticles and their activity against pathogenic bacteria

    Authors:
    M.Wypij, P. Golińska, H. Dahm, M. Rai
    Academic press:
    IET Nanobiotechnol (year: 2016, volume: --, pages: 46029), Publisher: --
    Status:
    Published
    DOI:
    10.1049/iet-nbt.2016.0112 – link to the publication
  8. Silver and gold nanoparticles synthesized from Streptomyces sp. isolated from acid forest soil with special reference to its antibacterial activity against pathogens

    Authors:
    M. Składanowski, M. Wypij, D. Laskowski, P. Golińska, H. Dahm, M. Rai
    Academic press:
    Journal of Cluster Science (year: 2017, volume: 28, pages: 59-79), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s10876-016-1043-6 – link to the publication
  9. Silver-lactoferrin nanocomplexes as a potent antimicrobial agent

    Authors:
    P. Pomastowski, M. Sprynskyy, P. Žuvela, K. Rafińska, M. Milanowski, J.J. Liu, M. Yi, B. Buszewski
    Academic press:
    Journal of the American Chemical Society (year: 2016, volume: 138, pages: 7899–7909), Publisher: ACS Publications
    Status:
    Published
    DOI:
    10.1021/jacs.6b02699 – link to the publication
  10. Synteza nanokompleksów srebrowo-kazeinowych/Synthesis of silver-casein nanocomplex

    Authors:
    P. Pomastowski, M. Sprynskyy, M. Słodowska, B. Buszewski
    Academic press:
    Przemysł Chemiczny (year: 2016, volume: 95(7), pages: 1420-1424), Publisher: NOT-SIGMA
    Status:
    Published
    DOI:
    10.15199/62.2016.7.29 – link to the publication
  11. Novel aspects of silver nanoparticles functionalization

    Authors:
    B. Buszewski, K. Rafińska, P. Pomastowski, J. Walczak, A. Rogowska
    Academic press:
    Colloids and Surfaces A: Physicochemical and Engineering Aspects (year: 2016, volume: 506, pages: 170-178), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.colsurfa.2016.05.058 – link to the publication
  12. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity

    Authors:
    D. Rathod, P. Golińska, M. Wypij, H. Dahm, M. Rai
    Academic press:
    Medical Microbiology and Immunology (year: 2016, volume: 205, pages: 435-447), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s00430-016-0462-1 – link to the publication
  13. Preparation of an improved hydrophilic monolith to make trypsin-immobilized microreactors

    Authors:
    K. Meller, P. Pomastowski, M. Szumski, B. Buszewski
    Academic press:
    Journal of Chromatography B (year: 2017, volume: 1043, pages: 128-137), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jchromb.2016.08.032 – link to the publication
  14. Acidophilic actinobacteria synthesized silver nanoparticles showed remarkable activity against fungi-causing superficial mycoses in humans

    Authors:
    N. Anasane, P. Golińska, M. Wypij, D. Rathod, H. Dahm, M. Rai
    Academic press:
    Mycoses (year: 2015, volume: 59(3), pages: 157-166), Publisher: Blacwell Verlag GmbH
    Status:
    Published
    DOI:
    10.1111/myc.12445 – link to the publication
  15. Endophytic actinobacteria of medicinal plants: diversity and bioactivity

    Authors:
    P. Golińska, M. Wypij, G. Agarkar, D. Rathod, H. Dahm, M. Rai
    Academic press:
    Antonie van Leeuwenhoek (year: 2015, volume: 108, pages: 267-289), Publisher: Springer
    Status:
    Published
  16. Assignment of functional groups in Gram-positive bacteria

    Authors:
    B. Buszewski, E. Dziubakiewicz, P. Pomastowski, K. Hrynkiewicz, J. Płoszaj-Pyrek, E. Talik, M. Kramer, K. Albert
    Academic press:
    Journal of Analytical & Bioanalytical Techniques (year: 2015, volume: 6(1), pages: 46030), Publisher: OMICS International
    Status:
    Published
    DOI:
    10.4172/2155-9872.1000232 – link to the publication
  17. Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry

    Authors:
    K .Rafińska, P. Pomastowski, O. Wrona, R. Górecki, B. Buszewski
    Academic press:
    Phytochemistry Letters (year: 2017, volume: 20, pages: 520-539), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.phytol.2016.12.006 – link to the publication
  18. Evaluation of intact cell matrix-assisted laser desorption/ionization time-of-flight mass spektrometry for capillary electrophoresis detection of controlled bacterial clumping

    Authors:
    P. Pomastowski, M. Szultka-Młyńska, W. Kupczyk, M. Jackowski, B. Buszewski
    Academic press:
    Journal of Analytical & Bioanalytical Techniques (year: 2015, volume: 13, pages: 46029), Publisher: OMICS International
    Status:
    Published
    DOI:
    10.4172/2155-9872.S13-008 – link to the publication
  19. Microextraction sample preparation techniques in biomedical analysis

    Authors:
    M. Szultka, P. Pomastowski, V. Railean-Plugaru, B. Buszewski
    Academic press:
    J. Sep. Sci. (year: 2014, volume: 37, pages: 3094-3105), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/jssc.201400621 – link to the publication
  20. HPLC separation of casein components on a diol-bonded silica column with MALDI TOF/TOF MS identification

    Authors:
    P. Pomastowski, J. Walczak, M. Gawin, S. Bocian, W. Piekoszewski, B. Buszewski
    Academic press:
    Analytical Methods (year: 2014, volume: 6, pages: 5236-5244), Publisher: RSC Publishing
    Status:
    Published
    DOI:
    10.1039/c5ay90054a – link to the publication
  21. Biogenic synthesis of metal nanoparticles from actinomycetes: biomedical applications and cytoxicity

    Authors:
    P. Golińska, M. Wypij, A. P. Ingle, I. Gupta, H. Dahm, M. Rai
    Academic press:
    Applied Microbiology & Biotechnology (year: 2014, volume: 98, pages: 8083-8097), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s00253-014-5953-7 – link to the publication
  22. Synthesis of silver nanoparticles from two acidophilic strains of Pilimelia columellifera subsp. pallida and their antibacterial activities

    Authors:
    P. Golinska, M. Wypij, D. Rathod, T. Sagar, H. Dahm, M. Rai
    Academic press:
    Journal of Basic Microbiology (year: 2015, volume: 55, pages: 46038), Publisher: Wiley-VCh Verlag GmbH & Co. KGaA, Wejnheim
    Status:
    Published
    DOI:
    10.1002/jobm.201500516 – link to the publication
  23. Actinospica durhamensis sp. nov., isolated from a spruce forest soil

    Authors:
    P. Golinska, T.D. Zucchi, L. Silva, H. Dahm, M. Goodfellow
    Academic press:
    Antonie van Leeuwenhoek (year: 2015, volume: 108, pages: 435-442), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s10482-015-0496-1 – link to the publication
  24. Study of in-vitro metabolism of selected antibiotic drugs in human liver microsomes by liquid chromatography coupled with tandem mass spectrometry

    Authors:
    M. Szultka-Młyńska, B. Buszewski
    Academic press:
    Analytical and Bioanalytical Chemistry (year: 2016, volume: 408(29), pages: 8273-8287), Publisher: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-016-9929-6 – link to the publication
  25. Antimicrobial activity of biosilver nanoparticles produced by novel Streptacidiphilus durhamensis strain

    Authors:
    B. Buszewski, V. Railean-Pugaru, P. Pomastowski, K. Rafińska, M. Szultka-Młyńska, P. Golińska, M. Wypij, D. Laskowski, H. Dahm
    Academic press:
    Journal of Microbiology, Immunology and Infection (year: 2018, volume: 51, pages: 45-54), Publisher: ScienceDirect
    Status:
    Published
    DOI:
    10.1016/j.jmii.2016.03.002 – link to the publication
  26. Nanoporous Conducting Polymer–Based Coatings in Microextraction Techniques for Environmental and Biomedical Applications

    Authors:
    M. Szultka-Młyńska, P. Olszowy, B. Buszewski
    Academic press:
    Critical Reviews in Analytical Chemistry (year: 2016, volume: 46(3), pages: 236-247), Publisher: Taylor&Francis
    Status:
    Published
    DOI:
    10.1080/10408347.2015.1081051 – link to the publication
  27. Preparation and evaluation of dual-enzyme microreactor with co-immobilized trypsyn and chymotrypsin

    Authors:
    K. Meller, P. Pomastowski, D. Grzywiński, M. Szumski, B. Buszewski
    Academic press:
    Journal of Chromatography A (year: 2016, volume: 1440, pages: 45-54), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.chroma.2016.02.070 – link to the publication
  28. Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil

    Authors:
    V. Railean-Plugaru, P. Pomastowski, M Wypij, M Szultka-Młyńska, K. Rafińska, P. Golińska, H. Dahm, B. Buszewski
    Academic press:
    Journal of Applied Microbiology (year: 2016, volume: 120 (5), pages: 1250-1263), Publisher: The Society for Applied Microbiology
    Status:
    Published
  29. Antimicrobial properties of biosynthesized silver nanoparticles studies by flow cytometry and related techniques

    Authors:
    V. Railean-Plugaru, P. Pomastowski, K. Rafińska, M. Wypij, W. Kupczyk, H. Dahm, M. Jackowski, B. Buszewski
    Academic press:
    Electrophoresis (year: 2016, volume: 37, pages: 752-761), Publisher: Wiley Online Library
    Status:
    Published
    DOI:
    10.1002/elps.201500507 – link to the publication
  30. Wpływ heterogeniczności powierzchni biokoloidów na ich rozdzielanie elektroforetyczne

    Authors:
    B. Buszewski, P. Pomastowski
    Academic press:
    Wiadomości Chemiczne (year: 2015, volume: 69 (9-10), pages: 823-846), Publisher: Polskie Towarzystwo Chemiczne
    Status:
    Published

Book publications / chapters in book publications (1)

  1. Microbial analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae using capillary electrophoresis approach

    Authors:
    P. Pomastowski, V. Railean-Plugaru, B. Buszewski
    Book:
    Capillary electrophoresis: methods and protocols / eds.: Philippe Schmitt-Kopplin (year: 2016, volume: 1483, pages: 393-406), Publisher: New York: Springer Science+Business Media
    Status:
    Published

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Graphic design and implementation: Jakub Piotrowski