Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Application of hyphenated separation techniques for fractionation of nanoparticles and metabolites of antibiotic nature produced by actinomycetes of extreme environments

2013/08/W/NZ8/00701

Keywords:

actinomycetes antibiotic metabolites silver nanoparticles in-vitro studies mass spectrometry

Descriptors:

  • NZ8_10: Taxonomy and phylogenetic
  • NZ8_4: Biodiversity
  • NZ8_13: Human biology and ecology

Panel:

NZ8 - Evolutionary and environmental biology: evolution, ecology, population biology, biodiversity, biogeography

Host institution :

Uniwersytet Mikołaja Kopernika, Wydział Chemii

woj. kujawsko-pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Bogusław Buszewski 

Number of co-investigators in the project: 12

Call: SYMFONIA 1 - announced on 2012-12-15

Amount awarded: 2 945 136 PLN

Project start date (Y-m-d): 2013-12-09

Project end date (Y-m-d): 2016-12-08

Project duration:: 36 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Wytrząsarka z inkubatorem wraz z platformami i uchwytami do mocowania kolb, MaxQ5000, Thermo Scientific (2 szt.) (100 000 PLN)
  2. waga analityczna (20 000 PLN)
  3. Laboratoryjna myjka ultradźwiękowa (6 500 PLN)
  4. agregat chłodzący do wyparki próżniowej (30 000 PLN)
  5. lodówka (2 000 PLN)
  6. Automatyczny wielokrotny inokulator wraz z głowicą i igłami inokulacyjnymi, Jencons PLS (60 000 PLN)
  7. Homogenizator mechaniczny - FastPrep -24 instrument, Thermoscientific (50 000 PLN)
  8. Klimatyzator (6 765 PLN)
  9. Niskotemperaturowa zamrażarka Forma 88000 Series, thermo Scientific (50 000 PLN)
  10. chromatograf cieczowy z detektorem uniwersalnym UV-Vis oraz detektorem fluorymetrycznym (250 000 PLN)
  11. koncentrator blokowy, do odparowania próbek (25 000 PLN)
  12. mieszadło obrotowe, plus uchwyty, Noria RD NR100DE (8 000 PLN)
  13. liofilizator (2 szt.) (170 000 PLN)
  14. pompa laboratoryjna próżniowa (3 500 PLN)
  15. termostat do automatycznego podajnika (30 000 PLN)
  16. urządzenie do termostatowania, przechowywania i transportowania próbek biologicznych (6 500 PLN)

Information in the final report

  • Publication in academic press/journals (31)
  • Book publications / chapters in book publications (1)
  1. Antimicrobial activity of biosilver nanoparticles produced by novel Streptacidiphilus durhamensis strain
    Authors:
    B. Buszewski, V. Railean-Pugaru, P. Pomastowski, K. Rafińska, M. Szultka-Młyńska, P. Golińska, M. Wypij, D. Laskowski, H. Dahm
    Academic press:
    Journal of Microbiology, Immunology and Infection (rok: 2018, tom: 51, strony: 45-54), Wydawca: ScienceDirect
    Status:
    Published
    DOI:
    10.1016/j.jmii.2016.03.002 - link to the publication
  2. Endophytic actinobacteria of medicinal plants: diversity and bioactivity
    Authors:
    P. Golińska, M. Wypij, G. Agarkar, D. Rathod, H. Dahm, M. Rai
    Academic press:
    Antonie van Leeuwenhoek (rok: 2015, tom: 108, strony: 267-289), Wydawca: Springer
    Status:
    Published
    DOI:
    10. - link to the publication
  3. HPLC separation of casein components on a diol-bonded silica column with MALDI TOF/TOF MS identification
    Authors:
    P. Pomastowski, J. Walczak, M. Gawin, S. Bocian, W. Piekoszewski, B. Buszewski
    Academic press:
    Analytical Methods (rok: 2014, tom: 6, strony: 5236-5244), Wydawca: RSC Publishing
    Status:
    Published
    DOI:
    10.1039/c5ay90054a - link to the publication
  4. Microextraction sample preparation techniques in biomedical analysis
    Authors:
    M. Szultka, P. Pomastowski, V. Railean-Plugaru, B. Buszewski
    Academic press:
    J. Sep. Sci. (rok: 2014, tom: 37, strony: 3094-3105), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/jssc.201400621 - link to the publication
  5. Preparation and evaluation of dual-enzyme microreactor with co-immobilized trypsyn and chymotrypsin
    Authors:
    K. Meller, P. Pomastowski, D. Grzywiński, M. Szumski, B. Buszewski
    Academic press:
    Journal of Chromatography A (rok: 2016, tom: 1440, strony: 45-54), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.chroma.2016.02.070 - link to the publication
  6. Study of in-vitro metabolism of selected antibiotic drugs in human liver microsomes by liquid chromatography coupled with tandem mass spectrometry
    Authors:
    M. Szultka-Młyńska, B. Buszewski
    Academic press:
    Analytical and Bioanalytical Chemistry (rok: 2016, tom: 408(29), strony: 8273-8287), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-016-9929-6 - link to the publication
  7. Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil
    Authors:
    V. Railean-Plugaru, P. Pomastowski, M Wypij, M Szultka-Młyńska, K. Rafińska, P. Golińska, H. Dahm, B. Buszewski
    Academic press:
    Journal of Applied Microbiology (rok: 2016, tom: 120 (5), strony: 1250-1263), Wydawca: The Society for Applied Microbiology
    Status:
    Published
    DOI:
    10,1111/jam.13093 - link to the publication
  8. Synthesis of silver nanoparticles from two acidophilic strains of Pilimelia columellifera subsp. pallida and their antibacterial activities
    Authors:
    P. Golinska, M. Wypij, D. Rathod, T. Sagar, H. Dahm, M. Rai
    Academic press:
    Journal of Basic Microbiology (rok: 2015, tom: 55, strony: 45307), Wydawca: Wiley-VCh Verlag GmbH & Co. KGaA, Wejnheim
    Status:
    Published
    DOI:
    10.1002/jobm.201500516 - link to the publication
  9. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity
    Authors:
    D. Rathod, P. Golińska, M. Wypij, H. Dahm, M. Rai
    Academic press:
    Medical Microbiology and Immunology (rok: 2016, tom: 205, strony: 435-447), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00430-016-0462-1 - link to the publication
  10. Antimicrobial activity of biosilver nanoparticles produced by novel Streptacidiphilus durhamensis strain
    Authors:
    B. Buszewski, V. Railean-Pugaru, P. Pomastowski, K. Rafińska, M. Szultka-Młyńska, P. Golińska, M. Wypij, D. Laskowski, H. Dahm
    Academic press:
    Journal of Microbiology, Immunology and Infection (rok: 2016, tom: --, strony: 45301), Wydawca: Taiwan Society of Microbiology, the Chinese Society of Immunology, The Infectious Diseases Society of Taiwan and the Taiwan Society of Parasitology
    Status:
    Published
    DOI:
    10.1016/j.jmii.2016.03.002 - link to the publication
  11. Evaluation of intact cell matrix-assisted laser desorption/ionization time-of-flight mass spektrometry for capillary electrophoresis detection of controlled bacterial clumping
    Authors:
    P. Pomastowski, M. Szultka-Młyńska, W. Kupczyk, M. Jackowski, B. Buszewski
    Academic press:
    Journal of Analytical & Bioanalytical Techniques (rok: 2015, tom: 13, strony: 45298), Wydawca: OMICS International
    Status:
    Published
    DOI:
    10.4172/2155-9872.S13-008 - link to the publication
  12. Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry
    Authors:
    K .Rafińska, P. Pomastowski, O. Wrona, R. Górecki, B. Buszewski
    Academic press:
    Phytochemistry Letters (rok: 2017, tom: 20, strony: 520-539), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.phytol.2016.12.006 - link to the publication
  13. Silver and gold nanoparticles synthesized from Streptomyces sp. isolated from acid forest soil with special reference to its antibacterial activity against pathogens
    Authors:
    M. Składanowski, M. Wypij, D. Laskowski, P. Golińska, H. Dahm, M. Rai
    Academic press:
    Journal of Cluster Science (rok: 2017, tom: 28, strony: 59-79), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10876-016-1043-6 - link to the publication
  14. Silver-lactoferrin nanocomplexes as a potent antimicrobial agent
    Authors:
    P. Pomastowski, M. Sprynskyy, P. Žuvela, K. Rafińska, M. Milanowski, J.J. Liu, M. Yi, B. Buszewski
    Academic press:
    Journal of the American Chemical Society (rok: 2016, tom: 138, strony: 7899–7909), Wydawca: ACS Publications
    Status:
    Published
    DOI:
    10.1021/jacs.6b02699 - link to the publication
  15. Electrochemistry-mass spectrometry for in-vitro determination of selected chemotherapeutics and their electrochemical products in comparison to in-vivo approach
    Authors:
    M. Szultka-Młyńska, B. Buszewski
    Academic press:
    Talanta (rok: 2016, tom: 160, strony: 694-703), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.talanta.2016.07.054 - link to the publication
  16. Novel aspects of silver nanoparticles functionalization
    Authors:
    B. Buszewski, K. Rafińska, P. Pomastowski, J. Walczak, A. Rogowska
    Academic press:
    Colloids and Surfaces A: Physicochemical and Engineering Aspects (rok: 2016, tom: 506, strony: 170-178), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.colsurfa.2016.05.058 - link to the publication
  17. Preparation of an improved hydrophilic monolith to make trypsin-immobilized microreactors
    Authors:
    K. Meller, P. Pomastowski, M. Szumski, B. Buszewski
    Academic press:
    Journal of Chromatography B (rok: 2017, tom: 1043, strony: 128-137), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jchromb.2016.08.032 - link to the publication
  18. Acidophilic actinobacteria synthesized silver nanoparticles showed remarkable activity against fungi-causing superficial mycoses in humans
    Authors:
    N. Anasane, P. Golińska, M. Wypij, D. Rathod, H. Dahm, M. Rai
    Academic press:
    Mycoses (rok: 2015, tom: 59(3), strony: 157-166), Wydawca: Blacwell Verlag GmbH
    Status:
    Published
    DOI:
    10.1111/myc.12445 - link to the publication
  19. Actinobacterial-mediated synthesis of silver nanoparticles and their activity against pathogenic bacteria
    Authors:
    M.Wypij, P. Golińska, H. Dahm, M. Rai
    Academic press:
    IET Nanobiotechnol (rok: 2016, tom: --, strony: 45298), Wydawca: --
    Status:
    Published
    DOI:
    10.1049/iet-nbt.2016.0112 - link to the publication
  20. Assignment of functional groups in Gram-positive bacteria
    Authors:
    B. Buszewski, E. Dziubakiewicz, P. Pomastowski, K. Hrynkiewicz, J. Płoszaj-Pyrek, E. Talik, M. Kramer, K. Albert
    Academic press:
    Journal of Analytical & Bioanalytical Techniques (rok: 2015, tom: 6(1), strony: 45299), Wydawca: OMICS International
    Status:
    Published
    DOI:
    10.4172/2155-9872.1000232 - link to the publication
  21. Nanoporous Conducting Polymer–Based Coatings in Microextraction Techniques for Environmental and Biomedical Applications
    Authors:
    M. Szultka-Młyńska, P. Olszowy, B. Buszewski
    Academic press:
    Critical Reviews in Analytical Chemistry (rok: 2016, tom: 46(3), strony: 236-247), Wydawca: Taylor&Francis
    Status:
    Published
    DOI:
    10.1080/10408347.2015.1081051 - link to the publication
  22. Synteza nanokompleksów srebrowo-kazeinowych/Synthesis of silver-casein nanocomplex
    Authors:
    P. Pomastowski, M. Sprynskyy, M. Słodowska, B. Buszewski
    Academic press:
    Przemysł Chemiczny (rok: 2016, tom: 95(7), strony: 1420-1424), Wydawca: NOT-SIGMA
    Status:
    Published
    DOI:
    10.15199/62.2016.7.29 - link to the publication
  23. Wpływ heterogeniczności powierzchni biokoloidów na ich rozdzielanie elektroforetyczne
    Authors:
    B. Buszewski, P. Pomastowski
    Academic press:
    Wiadomości Chemiczne (rok: 2015, tom: 69 (9-10), strony: 823-846), Wydawca: Polskie Towarzystwo Chemiczne
    Status:
    Published
    DOI:
    brak - link to the publication
  24. Actinospica durhamensis sp. nov., isolated from a spruce forest soil
    Authors:
    P. Golinska, T.D. Zucchi, L. Silva, H. Dahm, M. Goodfellow
    Academic press:
    Antonie van Leeuwenhoek (rok: 2015, tom: 108, strony: 435-442), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10482-015-0496-1 - link to the publication
  25. Actinospica durhamensis sp. nov., isolated from a spruce forest soil
    Authors:
    P. Golińska, T.D. Zucchi, L. Silva, H. Dahm, M. Goodfellow
    Academic press:
    Antonie van Leeuwenhoek (rok: 2015, tom: 108, strony: 435-442), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10482-015-0496-1 - link to the publication
  26. Antimicrobial effectiveness of synthesized bioactive silver nanoparticles by the Actinomycetes HGG16n strain
    Authors:
    B. Buszewski, V. Railean-Plugaru, P. Pomastowski, K. Rafińska, M. Szultka-Mlynska, T. Kowalkowski
    Academic press:
    Current Pharmaceutical Biotechnology (rok: 2017, tom: 18, strony: 168-176), Wydawca: Bentham Science Publishers
    Status:
    Published
    DOI:
    10.2174/13892010186661701041124 - link to the publication
  27. Antimicrobial properties of biosynthesized silver nanoparticles studies by flow cytometry and related techniques
    Authors:
    V. Railean-Plugaru, P. Pomastowski, K. Rafińska, M. Wypij, W. Kupczyk, H. Dahm, M. Jackowski, B. Buszewski
    Academic press:
    Electrophoresis (rok: 2016, tom: 37, strony: 752-761), Wydawca: Wiley Online Library
    Status:
    Published
    DOI:
    10.1002/elps.201500507 - link to the publication
  28. Biogenic synthesis of metal nanoparticles from actinomycetes: biomedical applications and cytoxicity
    Authors:
    P. Golińska, M. Wypij, A. P. Ingle, I. Gupta, H. Dahm, M. Rai
    Academic press:
    Applied Microbiology & Biotechnology (rok: 2014, tom: 98, strony: 8083-8097), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00253-014-5953-7 - link to the publication
  29. Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles
    Authors:
    M. Składanowski, P. Golińska, K. Rudnicka, H. Dahm, M. Rai
    Academic press:
    Medical Microbiology and Immunology (rok: 2016, tom: 205, strony: 603-613), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00430-016-0477-7 - link to the publication
  30. Mycoendophytes as efficient synthesizers of bionanoparticles: nanoantimicrobials, mechanism, and cytotoxicity
    Authors:
    P. Golińska, D. Rathod, M. Wypij, I. Gupta, M. Składanowski, P. Paralikar, H. Dahm, M. Rai
    Academic press:
    Critical Review in Biotechnology (rok: 2017, tom: 37, strony: 765-778), Wydawca: Taylor&Francis
    Status:
    Published
    DOI:
    10.1080/07388551.2016.1235011 - link to the publication
  31. Streptacidiphilus toruniensis sp. nov., isolated from a pine forest soil
    Authors:
    P. Golińska, H. Dahm, M. Goodfellow
    Academic press:
    Antonie van Leeuwenhoek (rok: 2016, tom: 109, strony: 1583-1591), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10482-016-0759-5 - link to the publication
  1. Microbial analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae using capillary electrophoresis approach
    Authors:
    P. Pomastowski, V. Railean-Plugaru, B. Buszewski
    Book:
    Capillary electrophoresis: methods and protocols / eds.: Philippe Schmitt-Kopplin (rok: 2016, tom: 1483, strony: 393-406), Wydawca: New York: Springer Science+Business Media
    Status:
    Published

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