Application of hyphenated separation techniques for fractionation of nanoparticles and metabolites of antibiotic nature produced by actinomycetes of extreme environments
2013/08/W/NZ8/00701
Keywords
Descriptors
- NZ8_10: Taksonomia i filogenetyka
- NZ8_4: Różnorodność biologiczna
- NZ8_13: Inne zagadnienia pokrewne
Panel
NZ8 – Evolutionary and environmental biology: evolution, ecology, population biology, biodiversity, biogeography
Host institution
Uniwersytet Mikołaja Kopernika, Wydział Chemii
Voivodeship: kujawsko-pomorskie
Principal investigator (from the host institution)
prof. Bogusław Andrzej Buszewski
Number of co-investigators in the project: 12
Call
SYMFONIA 1 – announced on 2012-12-15
- Amount awarded
- 2 945 136 PLN
- Project start date
- 2013-12-09
- Project end date
- 2016-12-08
- Project duration
- 36 months (the same as in the proposal)
- Project status
- Project settled
Equipment purchased [PL]
- Automatyczny wielokrotny inokulator wraz z głowicą i igłami inokulacyjnymi, Jencons PLS. Cost: 60 000 PLN
- Homogenizator mechaniczny - FastPrep -24 instrument, Thermoscientific. Cost: 50 000 PLN
- Klimatyzator. Cost: 6 765 PLN
- Laboratoryjna myjka ultradźwiękowa. Cost: 6 500 PLN
- Niskotemperaturowa zamrażarka Forma 88000 Series, thermo Scientific. Cost: 50 000 PLN
- Wytrząsarka z inkubatorem wraz z platformami i uchwytami do mocowania kolb, MaxQ5000, Thermo Scientific (2 pcs). Cost: 100 000 PLN
- agregat chłodzący do wyparki próżniowej. Cost: 30 000 PLN
- chromatograf cieczowy z detektorem uniwersalnym UV-Vis oraz detektorem fluorymetrycznym. Cost: 250 000 PLN
- koncentrator blokowy, do odparowania próbek. Cost: 25 000 PLN
- liofilizator (2 pcs). Cost: 170 000 PLN
- lodówka. Cost: 2 000 PLN
- mieszadło obrotowe, plus uchwyty, Noria RD NR100DE. Cost: 8 000 PLN
- pompa laboratoryjna próżniowa. Cost: 3 500 PLN
- termostat do automatycznego podajnika. Cost: 30 000 PLN
- urządzenie do termostatowania, przechowywania i transportowania próbek biologicznych. Cost: 6 500 PLN
- waga analityczna. Cost: 20 000 PLN
Information in the final report
Publication in academic press/journals (30)
-
Evaluation of cytotoxicity, immune compatibility and antibacterial activity of biogenic silver nanoparticles
- Authors:
- M. Składanowski, P. Golińska, K. Rudnicka, H. Dahm, M. Rai
- Academic press:
- Medical Microbiology and Immunology (year: 2016, volume: 205, pages: 603-613), Publisher: Springer
- Status:
- Published
- DOI:
- 10.1007/s00430-016-0477-7 – link to the publication
-
Antimicrobial effectiveness of synthesized bioactive silver nanoparticles by the Actinomycetes HGG16n strain
- Authors:
- B. Buszewski, V. Railean-Plugaru, P. Pomastowski, K. Rafińska, M. Szultka-Mlynska, T. Kowalkowski
- Academic press:
- Current Pharmaceutical Biotechnology (year: 2017, volume: 18, pages: 168-176), Publisher: Bentham Science Publishers
- Status:
- Published
- DOI:
- 10.2174/13892010186661701041124 – link to the publication
-
Mycoendophytes as efficient synthesizers of bionanoparticles: nanoantimicrobials, mechanism, and cytotoxicity
- Authors:
- P. Golińska, D. Rathod, M. Wypij, I. Gupta, M. Składanowski, P. Paralikar, H. Dahm, M. Rai
- Academic press:
- Critical Review in Biotechnology (year: 2017, volume: 37, pages: 765-778), Publisher: Taylor&Francis
- Status:
- Published
- DOI:
- 10.1080/07388551.2016.1235011 – link to the publication
-
Electrochemistry-mass spectrometry for in-vitro determination of selected chemotherapeutics and their electrochemical products in comparison to in-vivo approach
- Authors:
- M. Szultka-Młyńska, B. Buszewski
- Academic press:
- Talanta (year: 2016, volume: 160, pages: 694-703), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.talanta.2016.07.054 – link to the publication
-
Actinospica durhamensis sp. nov., isolated from a spruce forest soil
- Authors:
- P. Golińska, T.D. Zucchi, L. Silva, H. Dahm, M. Goodfellow
- Academic press:
- Antonie van Leeuwenhoek (year: 2015, volume: 108, pages: 435-442), Publisher: Springer
- Status:
- Published
- DOI:
- 10.1007/s10482-015-0496-1 – link to the publication
-
Streptacidiphilus toruniensis sp. nov., isolated from a pine forest soil
- Authors:
- P. Golińska, H. Dahm, M. Goodfellow
- Academic press:
- Antonie van Leeuwenhoek (year: 2016, volume: 109, pages: 1583-1591), Publisher: Springer
- Status:
- Published
- DOI:
- 10.1007/s10482-016-0759-5 – link to the publication
-
Actinobacterial-mediated synthesis of silver nanoparticles and their activity against pathogenic bacteria
- Authors:
- M.Wypij, P. Golińska, H. Dahm, M. Rai
- Academic press:
- IET Nanobiotechnol (year: 2016, volume: --, pages: 46029), Publisher: --
- Status:
- Published
- DOI:
- 10.1049/iet-nbt.2016.0112 – link to the publication
-
Silver and gold nanoparticles synthesized from Streptomyces sp. isolated from acid forest soil with special reference to its antibacterial activity against pathogens
- Authors:
- M. Składanowski, M. Wypij, D. Laskowski, P. Golińska, H. Dahm, M. Rai
- Academic press:
- Journal of Cluster Science (year: 2017, volume: 28, pages: 59-79), Publisher: Springer
- Status:
- Published
- DOI:
- 10.1007/s10876-016-1043-6 – link to the publication
-
Silver-lactoferrin nanocomplexes as a potent antimicrobial agent
- Authors:
- P. Pomastowski, M. Sprynskyy, P. Žuvela, K. Rafińska, M. Milanowski, J.J. Liu, M. Yi, B. Buszewski
- Academic press:
- Journal of the American Chemical Society (year: 2016, volume: 138, pages: 7899–7909), Publisher: ACS Publications
- Status:
- Published
- DOI:
- 10.1021/jacs.6b02699 – link to the publication
-
Synteza nanokompleksów srebrowo-kazeinowych/Synthesis of silver-casein nanocomplex
- Authors:
- P. Pomastowski, M. Sprynskyy, M. Słodowska, B. Buszewski
- Academic press:
- Przemysł Chemiczny (year: 2016, volume: 95(7), pages: 1420-1424), Publisher: NOT-SIGMA
- Status:
- Published
- DOI:
- 10.15199/62.2016.7.29 – link to the publication
-
Novel aspects of silver nanoparticles functionalization
- Authors:
- B. Buszewski, K. Rafińska, P. Pomastowski, J. Walczak, A. Rogowska
- Academic press:
- Colloids and Surfaces A: Physicochemical and Engineering Aspects (year: 2016, volume: 506, pages: 170-178), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.colsurfa.2016.05.058 – link to the publication
-
A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity
- Authors:
- D. Rathod, P. Golińska, M. Wypij, H. Dahm, M. Rai
- Academic press:
- Medical Microbiology and Immunology (year: 2016, volume: 205, pages: 435-447), Publisher: Springer
- Status:
- Published
- DOI:
- 10.1007/s00430-016-0462-1 – link to the publication
-
Preparation of an improved hydrophilic monolith to make trypsin-immobilized microreactors
- Authors:
- K. Meller, P. Pomastowski, M. Szumski, B. Buszewski
- Academic press:
- Journal of Chromatography B (year: 2017, volume: 1043, pages: 128-137), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.jchromb.2016.08.032 – link to the publication
-
Acidophilic actinobacteria synthesized silver nanoparticles showed remarkable activity against fungi-causing superficial mycoses in humans
- Authors:
- N. Anasane, P. Golińska, M. Wypij, D. Rathod, H. Dahm, M. Rai
- Academic press:
- Mycoses (year: 2015, volume: 59(3), pages: 157-166), Publisher: Blacwell Verlag GmbH
- Status:
- Published
- DOI:
- 10.1111/myc.12445 – link to the publication
-
Endophytic actinobacteria of medicinal plants: diversity and bioactivity
- Authors:
- P. Golińska, M. Wypij, G. Agarkar, D. Rathod, H. Dahm, M. Rai
- Academic press:
- Antonie van Leeuwenhoek (year: 2015, volume: 108, pages: 267-289), Publisher: Springer
- Status:
- Published
-
Assignment of functional groups in Gram-positive bacteria
- Authors:
- B. Buszewski, E. Dziubakiewicz, P. Pomastowski, K. Hrynkiewicz, J. Płoszaj-Pyrek, E. Talik, M. Kramer, K. Albert
- Academic press:
- Journal of Analytical & Bioanalytical Techniques (year: 2015, volume: 6(1), pages: 46030), Publisher: OMICS International
- Status:
- Published
- DOI:
- 10.4172/2155-9872.1000232 – link to the publication
-
Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry
- Authors:
- K .Rafińska, P. Pomastowski, O. Wrona, R. Górecki, B. Buszewski
- Academic press:
- Phytochemistry Letters (year: 2017, volume: 20, pages: 520-539), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.phytol.2016.12.006 – link to the publication
-
Evaluation of intact cell matrix-assisted laser desorption/ionization time-of-flight mass spektrometry for capillary electrophoresis detection of controlled bacterial clumping
- Authors:
- P. Pomastowski, M. Szultka-Młyńska, W. Kupczyk, M. Jackowski, B. Buszewski
- Academic press:
- Journal of Analytical & Bioanalytical Techniques (year: 2015, volume: 13, pages: 46029), Publisher: OMICS International
- Status:
- Published
- DOI:
- 10.4172/2155-9872.S13-008 – link to the publication
-
Microextraction sample preparation techniques in biomedical analysis
- Authors:
- M. Szultka, P. Pomastowski, V. Railean-Plugaru, B. Buszewski
- Academic press:
- J. Sep. Sci. (year: 2014, volume: 37, pages: 3094-3105), Publisher: Wiley
- Status:
- Published
- DOI:
- 10.1002/jssc.201400621 – link to the publication
-
HPLC separation of casein components on a diol-bonded silica column with MALDI TOF/TOF MS identification
- Authors:
- P. Pomastowski, J. Walczak, M. Gawin, S. Bocian, W. Piekoszewski, B. Buszewski
- Academic press:
- Analytical Methods (year: 2014, volume: 6, pages: 5236-5244), Publisher: RSC Publishing
- Status:
- Published
- DOI:
- 10.1039/c5ay90054a – link to the publication
-
Biogenic synthesis of metal nanoparticles from actinomycetes: biomedical applications and cytoxicity
- Authors:
- P. Golińska, M. Wypij, A. P. Ingle, I. Gupta, H. Dahm, M. Rai
- Academic press:
- Applied Microbiology & Biotechnology (year: 2014, volume: 98, pages: 8083-8097), Publisher: Springer
- Status:
- Published
- DOI:
- 10.1007/s00253-014-5953-7 – link to the publication
-
Synthesis of silver nanoparticles from two acidophilic strains of Pilimelia columellifera subsp. pallida and their antibacterial activities
- Authors:
- P. Golinska, M. Wypij, D. Rathod, T. Sagar, H. Dahm, M. Rai
- Academic press:
- Journal of Basic Microbiology (year: 2015, volume: 55, pages: 46038), Publisher: Wiley-VCh Verlag GmbH & Co. KGaA, Wejnheim
- Status:
- Published
- DOI:
- 10.1002/jobm.201500516 – link to the publication
-
Actinospica durhamensis sp. nov., isolated from a spruce forest soil
- Authors:
- P. Golinska, T.D. Zucchi, L. Silva, H. Dahm, M. Goodfellow
- Academic press:
- Antonie van Leeuwenhoek (year: 2015, volume: 108, pages: 435-442), Publisher: Springer
- Status:
- Published
- DOI:
- 10.1007/s10482-015-0496-1 – link to the publication
-
Study of in-vitro metabolism of selected antibiotic drugs in human liver microsomes by liquid chromatography coupled with tandem mass spectrometry
- Authors:
- M. Szultka-Młyńska, B. Buszewski
- Academic press:
- Analytical and Bioanalytical Chemistry (year: 2016, volume: 408(29), pages: 8273-8287), Publisher: Springer
- Status:
- Published
- DOI:
- 10.1007/s00216-016-9929-6 – link to the publication
-
Antimicrobial activity of biosilver nanoparticles produced by novel Streptacidiphilus durhamensis strain
- Authors:
- B. Buszewski, V. Railean-Pugaru, P. Pomastowski, K. Rafińska, M. Szultka-Młyńska, P. Golińska, M. Wypij, D. Laskowski, H. Dahm
- Academic press:
- Journal of Microbiology, Immunology and Infection (year: 2018, volume: 51, pages: 45-54), Publisher: ScienceDirect
- Status:
- Published
- DOI:
- 10.1016/j.jmii.2016.03.002 – link to the publication
-
Nanoporous Conducting Polymer–Based Coatings in Microextraction Techniques for Environmental and Biomedical Applications
- Authors:
- M. Szultka-Młyńska, P. Olszowy, B. Buszewski
- Academic press:
- Critical Reviews in Analytical Chemistry (year: 2016, volume: 46(3), pages: 236-247), Publisher: Taylor&Francis
- Status:
- Published
- DOI:
- 10.1080/10408347.2015.1081051 – link to the publication
-
Preparation and evaluation of dual-enzyme microreactor with co-immobilized trypsyn and chymotrypsin
- Authors:
- K. Meller, P. Pomastowski, D. Grzywiński, M. Szumski, B. Buszewski
- Academic press:
- Journal of Chromatography A (year: 2016, volume: 1440, pages: 45-54), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.chroma.2016.02.070 – link to the publication
-
Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil
- Authors:
- V. Railean-Plugaru, P. Pomastowski, M Wypij, M Szultka-Młyńska, K. Rafińska, P. Golińska, H. Dahm, B. Buszewski
- Academic press:
- Journal of Applied Microbiology (year: 2016, volume: 120 (5), pages: 1250-1263), Publisher: The Society for Applied Microbiology
- Status:
- Published
-
Antimicrobial properties of biosynthesized silver nanoparticles studies by flow cytometry and related techniques
- Authors:
- V. Railean-Plugaru, P. Pomastowski, K. Rafińska, M. Wypij, W. Kupczyk, H. Dahm, M. Jackowski, B. Buszewski
- Academic press:
- Electrophoresis (year: 2016, volume: 37, pages: 752-761), Publisher: Wiley Online Library
- Status:
- Published
- DOI:
- 10.1002/elps.201500507 – link to the publication
-
Wpływ heterogeniczności powierzchni biokoloidów na ich rozdzielanie elektroforetyczne
- Authors:
- B. Buszewski, P. Pomastowski
- Academic press:
- Wiadomości Chemiczne (year: 2015, volume: 69 (9-10), pages: 823-846), Publisher: Polskie Towarzystwo Chemiczne
- Status:
- Published
Book publications / chapters in book publications (1)
-
Microbial analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae using capillary electrophoresis approach
- Authors:
- P. Pomastowski, V. Railean-Plugaru, B. Buszewski
- Book:
- Capillary electrophoresis: methods and protocols / eds.: Philippe Schmitt-Kopplin (year: 2016, volume: 1483, pages: 393-406), Publisher: New York: Springer Science+Business Media
- Status:
- Published