Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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The effect of low-molecualar-weight antioxidants on photoreactivity of the age pigment lipofuscin and melanolipofuscin and their capacity to induce oxidative stress in retinal pigment epithelium

2013/08/A/NZ1/00194

Keywords:

age pigments lipofuscin melanolipofuscin retinal pigment epithelium (RPE) photoreactivity phototoxicity reactive oxygen species (ROS) antioxidants nanomechanical properties atomic force microscopy (AFM) age-related macular degeneration (AMD)

Descriptors:

  • NZ1_3: Biophysics
  • NZ3_4: Ageing
  • NZ3_1: Cell biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

UNIWERSYTET JAGIELLOŃSKI, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Tadeusz Sarna 

Number of co-investigators in the project: 4

Call: MAESTRO 4 - announced on 2012-12-15

Amount awarded: 2 996 000 PLN

Project start date (Y-m-d): 2013-08-28

Project end date (Y-m-d): 2019-08-27

Project duration:: 72 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. MINI-PROTEAN TETRA Cell.
  2. Mikroskop sił atomowych ze specjalistycznym oprogramowaniem do nanomanipulacji oraz nanolitografii (498 800 PLN)
  3. Głowica rotora/swing-out rotor wraz z koszykami i wkładami.
  4. Moduł Fotopowielacza typ H10330C-45 wraz z zasilaczem liniowym Voltcraft.
  5. Worteks MSV-3500 komplet z platformami (2 159 PLN)
  6. Zamrażarka niskotemperaturowa do -86C MDF-DU502VH (52 790 PLN)
  7. Komora inkubacyjna z przepływem cieczy z dedykowanym oprogramowaniem do monitorowania parametrów mechanicznych komórek (313 200 PLN)
  8. Oprogramowanie NOVA do potencjostatu PGSTAT 12/AUT 72406.
  9. Chłodziarka.
  10. Waga precyzyjna PS 200/2000.R1- zakr. 200/2000 g - dokł. 1/10mg.

Information in the final report

  • Publication in academic press/journals (14)
  1. Lipofuscin-mediated photic stress inhibits phagocytic activity of ARPE-19 cells; effect of donors' age and antioxidants.
    Authors:
    Olchawa Magdalena, Furso Justyna, Szewczyk Grzegorz, Sarna Tadeusz.
    Academic press:
    Free Radical Research (rok: 2017, tom: 51(9-10), strony: 799-811), Wydawca: TAYLOR & FRANCIS LTD
    Status:
    Published
    DOI:
    10.1080/10715762.2017.1380307. - link to the publication
  2. Photic Injury to Cultured RPE Varies Among Individual Cells in Proportion to Their Endogenous Lipofuscin Content as Modulated ny Their Melanosome Content
    Authors:
    Mariusz Zareba, Christine M.B. Skumatz, Tadeusz J. Sarna, and Janice M. Burke
    Academic press:
    Investigative Ophthalmology and Visual Science (rok: 2014, tom: 55, strony: 4982-4990), Wydawca: The Association for Research in Vision and Ophthalmology
    Status:
    Published
    DOI:
    10.1167iovs.14-14310 - link to the publication
  3. Photoaging of retinal pigment epithelial melanosomes: The effect of photobleaching on morphology, and reactivity of the pigment granules
    Authors:
    Zadlo Andrzej, Szewczyk Grzegorz, Sarna Michal, Kozinska Anna, Pilat Anna, Kaczara Patrycja, Sarna Tadeusz
    Academic press:
    Free Radical Biology and Medicine (rok: 2016, tom: 97, strony: 320-329), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.freeradbiomed.2016.06.012 - link to the publication
  4. Zeaxanthin and α-tocopherol reduce the inhibitory effects of photodynamic stress on phagocytosis by ARPE-19 cells
    Authors:
    Olchawa MM, Herrnreiter AM, Pilat AK, Skumatz CM, Niziolek-Kierecka M, Burke JM, Sarna TJ.
    Academic press:
    Free Radical Bioogy andl Medicine (rok: 2015, tom: 89, strony: 873-882), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.411 - link to the publication
  5. Photobleaching of pheomelanin increases its phototoxic potential: Physicochemical studies of synthetic pheomelanin subjected to aerobic photolysis.
    Authors:
    Zadlo Andrzej, Szewczyk Grzegorz, Sarna Michał, Camenisch TG, Sidabras JW, Ito S, Wakamatsu K, Sagan F, Mitoraj M, Sarna Tadeusz.
    Academic press:
    Pigment Cell and Melanoma Research (rok: 2018, tom: -, strony: -), Wydawca: John Wiley & Sons Ltd
    Status:
    Published
    DOI:
    10.1111/pcmr.12752 - link to the publication
  6. Photodegradation of Eumelanin and Pheomelanin and Its Pathophysiological Implications.
    Authors:
    Ito S, Wakamatsu K, Sarna Tadeusz
    Academic press:
    Photochemistry and Photobiology (rok: 2017, tom: -, strony: -), Wydawca: WILEY-BLACKWELL
    Status:
    Published
    DOI:
    10.1111/php.12837 - link to the publication
  7. The nanomechanical role of melanin granules in the retinal pigment epithelium.
    Authors:
    Sarna Michał, Olchawa Magdalena, Zadlo Andrzej, Wnuk Dawid, Sarna Tadeusz.
    Academic press:
    Nanomedicine: Nanotechnology Biology and Medicine (rok: 2017, tom: 13(3), strony: 801-807), Wydawca: ELSEVIER SCIENCE BV
    Status:
    Published
    DOI:
    10.1016/j.nano.2016.11.020. - link to the publication
  8. Aerobic photoreactivity of synthetic eumelanins and pheomelanins: generation of singlet oxygen and superoxide anion.
    Authors:
    Szewczyk Grzegorz, Zadlo Andrzej, Sarna Michal, Ito S., Wakamatsu K., Sarna Tadeusz
    Academic press:
    Pigment Cell and Melanoma Research (rok: 2016, tom: 29(6), strony: 669-678), Wydawca: John Wiley & Sons Ltd
    Status:
    Published
    DOI:
    10.1111/pcmr.12514 - link to the publication
  9. Inhibition of phagocytic activity of ARPE-19 cells by free radical mediated oxidative stress
    Authors:
    Olchawa Magdalena, Pilat Anna, Szewczyk Grzegorz, Sarna Tadeusz
    Academic press:
    Free Radical Research (rok: 2016, tom: 50(8), strony: 887-897), Wydawca: Taylor & Francis Online
    Status:
    Published
    DOI:
    10.1080/10715762.2016.1194519. - link to the publication
  10. Lipofuscin-mediated photodynamic stress induces adverse changes in nanomechanical properties of retinal pigment epithelium cells.
    Authors:
    Wiktor Anna, Sarna Michal, Wnuk D, Sarna Tadeusz.
    Academic press:
    Scientific Reports - Nature (rok: 2018, tom: 18, strony: -), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-018-36322-2 - link to the publication
  11. Photoreactivity of Bis-retinoid A2E Complexed with a Model Protein in Selected Model Systems
    Authors:
    Furso Justyna, Zadlo Andrzej, Szewczyk Grzegorz, Sarna Tadeusz J
    Academic press:
    Cell Biochemistry and Biophysics (rok: 2020, tom: 78, strony: 415-427), Wydawca: HUMANA PRESS INC 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA
    Status:
    Published
    DOI:
    10.1007/s12013-020-00942-1 - link to the publication
  12. The Effect of Antioxidants on Photoreactivity and Phototoxic Potential of RPE Melanolipofuscin Granules from Human Donors of Different Age
    Authors:
    Olchawa Magdalena M, Szewczyk Grzegorz M, Zadlo Andrzej C,Sarna Michal W, Wnuk Dawid, Sarna Tadeusz J
    Academic press:
    Antioxidants (Basel) (rok: 2020, tom: 9, strony: 45313), Wydawca: MDPI ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
    Status:
    Published
    DOI:
    10.3390/antiox9111044 - link to the publication
  13. Roles of reactive oxygen species in UVA-induced oxidation of 5,6-dihydroxyindole-2-carboxylic acid-melanin as studied by differential spectrophotometric method
    Authors:
    Ito S., Kikuta M., Koike S., Szewczyk Grzegorz, Sarna Michal, Zadlo Andrzej, Sarna Tadeusz, Wakamatsu K.
    Academic press:
    Pigment Cell and Melanoma Research (rok: 2016, tom: 29(3), strony: 340-351), Wydawca: John Wiley & Sons Ltd
    Status:
    Published
    DOI:
    10.1111/pcmr.12469 - link to the publication
  14. The effect of aging and antioxidants on photoreactivity and phototoxicity of human melanosomes: An in vitro study
    Authors:
    Olchawa Magdalena M, Szewczyk Grzegorz M, Zadlo Andrzej C,Krzysztynska-Kuleta Olga I, Sarna Tadeusz J
    Academic press:
    Pigment Cell and Melanoma Research (rok: 2020, tom: -, strony: 45305), Wydawca: WILEY 111 RIVER ST, HOBOKEN 07030-5774, NJ Denmark
    Status:
    Published
    DOI:
    10.1111/pcmr.12914. - link to the publication

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