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Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Factors modyfying cultured pig embryos: Cytological and biotechnological implications.

2012/07/B/NZ9/01326

Keywords:

pig blastocysts culture osmotic stress oxidative sress high hydrostatic pressure stress hypoxia hyaluronic acid

Descriptors:

  • NZ9_2: Animal production
  • NZ1_9: Biotechnology
  • NZ3_13: Developmental biology in animals

Panel:

NZ9 - Fundamentals of applied life sciences and biotechnology: agricultural, forestry, horticulture, animal production and fishery, food and nutrition sciences, industrial biosciences, environmental biotechnology and remediation

Host institution :

Uniwersytet Jagielloński w Krakowie, Wydział Biologii i Nauk o Ziemi

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Marek Romek 

Number of co-investigators in the project: 8

Call: OPUS 4 - announced on 2012-09-15

Amount awarded: 865 730 PLN

Project start date (Y-m-d): 2013-07-18

Project end date (Y-m-d): 2017-07-17

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Chłodziarko-zamrażarka.
  2. Komputer stacjonarny (3 495 PLN)
  3. Nóż diamentowy do krojenia półcienkich skrawków (18 000 PLN)
  4. Nóż diamentowy do trymowania bloczków (18 000 PLN)
  5. Laminar flow, 1,8 m ULPA, II klasy bezpieczeństwa (60 000 PLN)
  6. Wirówka stołowa (947 PLN)
  7. 2 cieplarki laboratoryjne.
  8. Inkubator CO2/O2/N2 (65 000 PLN)
  9. Naczynie Dewara.
  10. Nóż diamentowy do krojenia półcienkich skrawków (20 700 PLN)
  11. Przenośny inkubator do przewozu zarodków (30 000 PLN)

Information in the final report

  • Publication in academic press/journals (2)
  • Articles in post-conference publications (14)
  1. Improved quality of porcine embryos cultured with hyaluronan due to the modification of the mitochondrial membrane potential and reactive oxygen species level
    Authors:
    Romek M, Gajda B, Krzysztofowicz E, Kucia M, Użarowska A, Smorag Z
    Academic press:
    Theriogenology (rok: 2017, tom: 102, strony: 45300), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.theriogenology.2017.06.026 - link to the publication
  2. Effect of high hydrostatic pressure on mitochondrial activity, reactive oxygen species level and developmental competence of cultured pig embryos
    Authors:
    Marek Romek, Marcin Kucia, Barbara Gajda, Ewa Krzysztofowicz, Zdzislaw Smorag
    Academic press:
    Theriogenology (rok: 2019, tom: 140, strony: 99-108), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.theriogenology.2019.08.013 - link to the publication
  1. Efect of hyaluronic acid on the inner mitochondrial membrane potential in cultured pig embryos
    Authors:
    Romek M, Gajda B, Kucia M, Smorąg Z
    Conference:
    Progress in Cell Biology: Mitochondria and Chloroplasts (rok: 2015, ), Wydawca: The Commitetee of Embryology and Morphology of the Polish Academy of Arts and Sciences
    Data:
    konferencja 29 maja
    Status:
    Published
  2. Effect of low oxygen tension on mitochondrial activity in cultured pig embrryos
    Authors:
    Romek M, Gajda B, Smorąg Z
    Conference:
    31st Meeting of the European Embryo Transfer Association (rok: 2015, ), Wydawca: Colégio Brasileiro de Reprodução Animal
    Data:
    konferencja 20-23 września
    Status:
    Published
  3. Impact of osmotic stress on mitochondrial activity in porcine blastocysts
    Authors:
    Romek M, Gajda B, Krzysztofowicz E, Smorąg Z
    Conference:
    Progress in Cell Biology: Mitochondria and Chloroplasts (rok: 2015, ), Wydawca: The Commitetee of Embryology and Morphology of the Polish Academy of Arts and Sciences
    Data:
    konferencja 29 maja
    Status:
    Published
  4. Reactive oxygen species level in pig embryos cultured in presence of hyaluronan
    Authors:
    Gajda B., Kucia M., Smorąg Z., Romek M.
    Conference:
    42nd Annual Conference of the International Embryo Transfer Society (rok: 2016, ), Wydawca: CSIRO Publishing
    Data:
    konferencja 23-26 stycznia
    Status:
    Published
    DOI:
    link to the publication
  5. Volume density of mitochondria in cultured porcine blastocysts
    Authors:
    Romek M, Gajda B, Smorąg Z
    Conference:
    Progress in Developmental Biology (rok: 2017, ), Wydawca: The Commitetee of Embryology and Morphology of the Polish Academy of Arts and Sciences
    Data:
    konferencja 26 maja
    Status:
    Published
  6. Wpływ kwasu hialuronowego oraz wysokiego ciśnienia hydrostatycznego (HHP) na rozwój in vitro zarodków świni
    Authors:
    Romek M, Krzysztofowicz E, Gajda B, Smorąg Z
    Conference:
    VII Zjazd Towarzystwa Biologii Rozrodu (rok: 2014, ), Wydawca: Towarzystwo Biologii Rozrodu
    Data:
    konferencja 11-13 września 2014 155
    Status:
    Published
  7. Content of lipid droplets in pig embryos after high hydrostatic pressure stress
    Authors:
    Romek M, Kucia M, Waligórski Z, Gajda B, Smorąg Z
    Conference:
    Progress in Developmental Biology (rok: 2017, ), Wydawca: The Commitetee of Embryology and Morphology of the Polish Academy of Arts and Sciences
    Data:
    konferencja 26 maja
    Status:
    Published
  8. The role of mitochondria during early development of pig embryos
    Authors:
    Kucia M, Gajda B, Romek M
    Conference:
    Progress in Cell Biology: Mitochondria and Chloroplasts (rok: 2015, ), Wydawca: The Commitetee of Embryology and Morphology of the Polish Academy of Arts and Sciences
    Data:
    konferencja 29 maja
    Status:
    Published
  9. Lipidy w oocytach i zarodkach świni; implikaje dla biotechnologii
    Authors:
    Romek M
    Conference:
    VII Zjazd Towarzystwa Biologii Rozrodu (rok: 2014, ), Wydawca: Towarzystwo Biologii Rozrodu
    Data:
    konferencja 11-13 września
    Status:
    Published
  10. Influence of sublethal, high hydrostatic pressure stress on embryo quality and long-term response of mitochondrial activity in porcine blastocyst
    Authors:
    Kucia M, Gajda B, Krzysztofowicz E, Smorąg Z, Romek M
    Conference:
    Progress in Cell Biology: Mitochondria and Chloroplasts (rok: 2015, ), Wydawca: The Commitetee of Embryology and Morphology of the Polish Academy of Arts and Sciences
    Data:
    konferencja 29 maja
    Status:
    Published
  11. Reactive oxygen species level in cultured embryos after high hydrostatic pressure
    Authors:
    Romek M., Kucia M., Gajda B., Smorąg Z.
    Conference:
    42nd Annual Conference of the International Embryo Transfer Society (rok: 2016, ), Wydawca: CSIRO Publishing
    Data:
    konferencja 23-26 stycznia
    Status:
    Published
    DOI:
    link to the publication
  12. Fluorescent method for quantitative analysis of lipid composition in single mammalian oocytes and embryos
    Authors:
    Romek M
    Conference:
    XLIX Sympozjum Polskiego Towarzystwa Histochemików i Cytochemików. Współczesne techniki mikroskopowe i molekularne w biologii i medycynie (rok: 2015, ), Wydawca: Polskie Towarzystwo Histochemików i Cytochemików
    Data:
    konferencja 9-12 września
    Status:
    Published
  13. Impact of different culture systems on lipid droplet content in pig blastocysts
    Authors:
    Romek M, Gajda B, Smorąg Z
    Conference:
    Progress in Developmental Biology (rok: 2017, ), Wydawca: The Commitetee of Embryology and Morphology of the Polish Academy of Arts and Sciences
    Data:
    konferencja 26 maja
    Status:
    Published
  14. Reactive oxygen species level in pig embryos cultured in hypoxic condition
    Authors:
    Romek M., Gajda B., Rajska I., Smorąg Z.
    Conference:
    32nd Meeting of the European Embryo Transfer Association (rok: 2016, ), Wydawca: Colégio Brasileiro de Reprodução Animal
    Data:
    konferencja 9-10 wrzesień
    Status:
    Published

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