Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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New mechanizm of regulation of gene expression of MboII restriction-modification system from Moraxella bovis

2012/07/B/NZ2/01782

Keywords:

regulation of gene expression DNA restriction-modification translational frameshifting genetic stability

Descriptors:

  • NZ2_1: Molecular genetics
  • NZ1_1: Molecular biology
  • NZ1_10: Microbiology

Panel:

NZ2 - Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology

Host institution :

Uniwersytet Gdański, Wydział Biologii

woj. pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Marian Sęktas 

Number of co-investigators in the project: 4

Call: OPUS 4 - announced on 2012-09-15

Amount awarded: 369 100 PLN

Project start date (Y-m-d): 2013-07-05

Project end date (Y-m-d): 2016-12-04

Project duration:: 41 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. wodna wytrząsarka stołowa na kolby Erlenmayera (50 000 PLN)
  2. mikrowirówka na probówki Eppendorfa z chłodzeniem (18 000 PLN)
  3. mikrowirówka.
  4. termoblok (4 000 PLN)
  5. aparat do mini-żeli poliakryloamidowych.
  6. aparat do półsuchego transferu białek.
  7. kolektor frakcji wraz z pompą perystaltyczną nikskocisnieniową.
  8. suchy blok grzejny 2 sztuki (2 349 PLN)
  9. termocycler (15 000 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  1. Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression
    Authors:
    Wons E., Koscielniak D., Szadkowska M., Sektas M.
    Academic press:
    Microbial Cell Factories (rok: 2018, tom: 17(1):150, strony: 45301), Wydawca: BMC
    Status:
    Published
    DOI:
    10.1186/s12934-018-0999-3 - link to the publication
  2. RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes
    Authors:
    Wons E., B. Furmanek-Blaszk, M. Sektas
    Academic press:
    Nucleic Acids Research (rok: 2015, tom: 43, strony: 3950-3963), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkv269 - link to the publication
  3. Improvement in the visual discrimination of recombinant clones by size reduction of non-recombinant colonies
    Authors:
    Sektas M, Furmanek-Blaszk B.
    Academic press:
    J Microbiol Methods (rok: 2013, tom: 95, strony: 302-303), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.mimet.2013.09.003. - link to the publication
  4. Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent
    Authors:
    Dawid Koscielniak, Ewa Wons, Karolina Wilkowska, Marian Sektas
    Academic press:
    Microbial Cell Factories (rok: 2018, tom: MICF-D-18-00268, strony: ), Wydawca: BMC
    Status:
    Submitted
    DOI:
    MICF-D-18-00268 - link to the publication

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