Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Spatiotemporal dynamics of Rho GTPase activity during electrotaxis of cells representing different strategy of cell migration

2012/07/B/NZ3/02909

Keywords:

electrotaxis cell migration strategies Rho proteins reactive oxigen species calcium ions

Descriptors:

  • NZ3_1: Cell biology
  • NZ3_2: Cell physiology
  • NZ3_7: Signal transduction

Panel:

NZ3 - Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution :

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Zbigniew Madeja 

Number of co-investigators in the project: 6

Call: OPUS 4 - announced on 2012-09-15

Amount awarded: 435 400 PLN

Project start date (Y-m-d): 2013-07-10

Project end date (Y-m-d): 2017-07-09

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Kamera CCD.
  2. komputer.
  3. inkubator do hodowli komórek (35 000 PLN)

Information in the final report

  • Publication in academic press/journals (8)
  • Articles in post-conference publications (6)
  • Book publications / chapters in book publications (1)
  1. 9-AAA inhibits growth and induces apoptosis in human melanoma A375 and rat prostate adenocarcinoma AT-2 and Mat-LyLu cell lines but does not affect the growth and viability of normal fibroblasts
    Authors:
    Włodzimierz Korohoda, Anna Hapek, Monika Pietrzak, Damian Ryszawy, Zbigniew Madeja
    Academic press:
    Oncology Letters (rok: 2016, tom: 12(5), strony: 4125-4132), Wydawca: Spandidos Publication
    Status:
    Published
    DOI:
    10.3892/ol.2016.5201 - link to the publication
  2. Efficacy of Local Anesthetics in Detachment of Normal 3T3Mouse Fibroblasts and Prostate Cancer AT-2 Cells from Substrata, in Maintenance of Viable Cells in a Non-Adherent State, and in Preservation of Cell Surface Markers Detected with FlowSight Image Cytometry
    Authors:
    Włodzimierz KOROHODA, Jarosław OCZKOWICZ, Kamil ROLSKI, Damian RYSZAWY, Zbigniew MADEJA
    Academic press:
    Folia Biologica(Kraków) (rok: 2015, tom: 63,No 4, strony: 249-255), Wydawca: Institute of Systematics and Evolution of Animals,PAS,Kraków
    Status:
    Published
    DOI:
    10.3409/fb63_4.249 - link to the publication
  3. Migration-related protein activity in cell electrotaxis_
    Authors:
    Zbigniew Baster, Slawomir Lasota, Tomasz Witko, Eliza Zimolaag, Jolanta Sroka, Zbigniew Madeja, Zenon Rajfur
    Academic press:
    Acta Physica Polonica B (rok: 2017, tom: 48, strony: 1727-1730), Wydawca: Jagiellonian University, Cracow, Poland in cooperation with the Polish Academy of Arts and Sciences
    Status:
    Published
    DOI:
    10.5506/APhysPolB.48.1727 - link to the publication
  4. Zastosowanie biosensorów typu FRET w badaniach mikroskopowych procesu migracji komórkowej
    Authors:
    Lasota S, Baster Z, Witko T, Zimoląg E, Sroka J, Rajfur Z, Madeja Z
    Academic press:
    Postępy Biochemii (rok: 2017, tom: 63(1), strony: 16-33), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
    DOI:
    brak - link to the publication
  5. Avoiding the side effects of electric current pulse application to electroporated cells in disposable small volume cuvettes assures good cell survival
    Authors:
    Grys M, Madeja Z, Korohoda W
    Academic press:
    Cellular and Molecular Biology Letters (rok: 2017, tom: 13, strony: 45304), Wydawca: BioMed Central System
    Status:
    Published
    DOI:
    10.1186/s11658-016-0030-0 - link to the publication
  6. Lamellipodia and Membrane Blebs Drive Efficient Electrotactic Migration of Rat Walker Carcinosarcoma Cells WC 256
    Authors:
    Jolanta Sroka , Izabela Krecioch, Eliza Zimolag, Slawomir Lasota, Monika Rak, Sylwia Kedracka-Krok, Pawel Borowicz, Marta Gajek, Zbigniew Madeja
    Academic press:
    Plos One (rok: 2016, tom: 10;11(2), strony: e0149133), Wydawca: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0149133
    Status:
    Published
    DOI:
    10.1371/journal.pone.0149133 - link to the publication
  7. The role of microtubules in electrotaxis of rat Walker carcinosarcoma WC256 cells.
    Authors:
    Krecioch I, Madeja Z, Lasota S, Zimolag E, Sroka J
    Academic press:
    Acta Biochimica Polonica (rok: 2015, tom: 62(3), strony: 401-406), Wydawca: The Journal of the Polish Biochemical Society and of the Committee of Biochemistry and Biophysics Polish Academy of Sciences
    Status:
    Published
    DOI:
    10.18388/abp.2015_1019 - link to the publication
  8. Electric field as a potential directional cue in homing of bone marrow-derived mesenchymal stem cells to cutaneous wounds
    Authors:
    Eliza Zimolag, Julia Borowczyk-Michalowska, Sylwia Kedracka-Krok, Bozena Skupien-Rabian, Elzbieta Karnas, Slawomir Lasota, Jolanta Sroka, Justyna Drukala, Zbigniew Madeja
    Academic press:
    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (rok: 2017, tom: 1864(2), strony: 267-279 Epub 2016 Nov15), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbamcr.2016.11.011 - link to the publication
  1. Dynamics of mouse embryonic fibroblasts respose to direct current electric field
    Authors:
    Lasota S, Sroka J, Zimoląg E, Madeja Z
    Conference:
    International Student Conference of Cell Biology (rok: 2016, ), Wydawca: Uniwersytet Jagielloński
    Data:
    konferencja 1-2 kwietnia
    Status:
    Published
  2. The involvement of ATP release and purinergic receptors in electrotaxis of mouse bone marrow-derived mesenchymal stem cells and macrophages
    Authors:
    E. Zimolag, J. Borowczyk-Michalczewska, S. Lasota, J. Sroka, J. Drukała, Z. Madeja
    Conference:
    "No stress-no life"XLIV Winter School Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University (rok: 2017, ), Wydawca: Jagiellonian University
    Data:
    konferencja 14-18 February 2017, Zakopane
    Status:
    Published
    DOI:
    link to the publication
  3. Protein activation dynamics measurements in migrating cells using FRET in time domain
    Authors:
    Z. Baster, S. Lasota, T. Witko, E. Zimolag, J. Sroka. Z. Madeja, L. Hodgson, Z. Rajfur
    Conference:
    III International Conference of Cell Biology (rok: 2017, ), Wydawca: Jagiellonian University
    Data:
    konferencja 26-27 May
    Status:
    Published
    DOI:
    link to the publication
  4. L3417-Pos/LB72-migration-related protein activity in cell electrotaxis
    Authors:
    Z. Baster, S. Lasota, T. Witko, E. Zimolag, J. Sroka, Z. Madeja, Z. Rajfur
    Conference:
    Biophysical Society 61st Annual Meeting (rok: 2017, ), Wydawca: Biohysical Society
    Data:
    konferencja 11-15 February, New Orlean, United States
    Status:
    Published
    DOI:
    link to the publication
  5. Protein activation dynamics measurements in migrating cells using FRET in time domain
    Authors:
    Baster Z, Lasota S, Witko T, Zimoląg E, Sroka J, Madeja Z, Hodgson L, Rajfur Z
    Conference:
    11th Workshop and Conference on Advanced Multhiphoton and Fluorescence Lifetime Imaging Techniques (rok: 2016, ), Wydawca: Biocev
    Data:
    konferencja 13-15 czerwca
    Status:
    Published
  6. Lamellipodia and membrane blebs utilize different signalling pathways to induce directional movement of Walker Carcinosarcoma WC 256 cells in a physiological electric field
    Authors:
    Sroka J, Zimolag E, Piwowarczyk, K, Lasota S, Kedracka-Krok S, Jankowska U, Madeja Z
    Conference:
    FEBS (rok: 2016, ), Wydawca: Wiley Online Library
    Data:
    konferencja została odwołana ze względu na sytuację wewnętrzną w Turcji
    Status:
    Published
    DOI:
    link to the publication
  1. Electrotaxis: cell directional movement in electric fields
    Authors:
    Jolanta Sroka, Eliza Zimolag, Slawomir Lasota, Wlodzimierz Korohoda, Zbigniew Madeja
    Book:
    Methods in Molecular Biology (rok: 2018, tom: 1749, strony: 325-340), Wydawca: Springer
    Status:
    Published
    DOI:
    link to the publication

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